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Human sperm quality and lipid content after migration into normal ovulatory human cervical mucus containing low numbers of leukocytes

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Asian Journal of Andrology 2009年第3期11卷 308-316页

作者： Nozha chakroun-Feki Patrice Therond Martine couturier Florence Eustache Gerard Limea alain Legrand Pierre Jouannet Jacques Auger Laboratory of Reproductive Biology/cEcOS （center of Study and conservation of human Eggs and Sperm） Cochin Hospital Rend Descartes University Paris 75014 France Laboratory of clinical and Metabolic Biochemistry EA 3617 Rend Descartes University Paris 75270 Cedex 06 France INSERM U551 La Pitie Hospital 75651 Paris Cedex 13 France

The aim of this study was to investigate whether a relationship exists between the presence of low numbers of leukocytes in normal ovulatory cervical mucus and sperm quality and lipid content after migration. The percentages of live, motile and morphologically normal spermatozoa, movement parameters assessed by computer-aided sperm analysis （cASA）, and ionophore-induced acrosome reaction measured by flow cytometry were determined before and after migration. High-performance liquid chromatography with ultraviolet detection was used to measure the sperm lipid content, including the various diacyl subspecies. The number of leukocytes found in solubilized mucus samples was counted using a haemocytometric method. Overall, the presence of leukocytes in the cervical mucus samples did not significantly influence sperm motility and morphology, sperm kinematic parameters, or the sperm content in sphingomyelin or cholesterol. In contrast, after migration, the decrease in various sperm diacyls and the level of induced acrosome reaction was significantly less pronounced in mucus samples containing ≥10^4 leukocytes than in mucus samples with no or rare leukocytes whereas the level of induced acrosome reaction was higher. The present data suggest that the low level of leukocytes found in normal ovulatory cervical mucus could influence the process of sperm lipid remodelling/capacitation.

关键词： cervical mucus leukocyte lipid spermatozoa

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Anti-IgE IgG autoantibodies isolated from therapeutic normal IgG intravenous immunoglobulin induce basophil activation

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cellular & Molecular Immunology 2020年第4期17卷 426-429页

作者： caroline Galeotti Anupama Karnam Jordan D.Dimitrov alain chevailler Srini V.Kaveri Jagadeesh Bayry Institut National de la Santéet de la Recherche Médicale/centre de Recherche des cordeliers Equipe-Immunopathologie et Immunointervention ThérapeutiqueSorbonne UniversitéParis F-75006France Service de Rhumatologie Pédiatrique Centre de Référence des Maladies Auto-Inflammatoires Rares et des AmylosesCHU de Bicêtrele Kremlin Bicêtre F-94270France UniversitéParis Descartes Sorbonne Paris CitéParis F-75006France Laboratoire d’Immunologie et d’Allergologie/cHU d’Angers Universitéd’Angers/INSERM Unité1232/LabEx IGO“Immuno-Graft-Onco”Angers F-49933France

Basophils are rare *** representing only~0.5%of all leukocytes,basophils have several important physiological functions.1,2 Although basophils lack the classic features of professional antigen-presenting cells,3–7 through the secretion of cytokines,they orient the immune response by polarizing Th2 differentiation and supporting B-cell differentiation and class *** are also critical for mediating protection against helminth infection.1,2,8,9 Basophils receive activation signals from diverse *** is well recognized that cytokines such as IL-3,granulocyte–macrophage colony-stimulating factor(GM-cSF),thymic stromal lymphopoietin(TSLP)and IL-33;various toll-like receptor ligands;allergen-bound IgE provide activation signals to basophils and induce the release of inflammatory mediators.10–15 In addition,several reports have also demonstrated the existence of anti-IgE autoantibodies that possess the capacity to induce basophil activation in patients with chronic spontaneous urticaria(cSU),atopic or non-atopic asthma or autoimmune disease.16–21 However,isolation and functional exploration of such anti-IgE IgG autoantibodies from either healthy donors or patients have not been attempted *** using a pooled normal IgG preparation from healthy donors,specifically intravenous immunoglobulin G(IVIG)22 that represents the complete IgG repertoire of a normal individual,we attempted to address this outstanding question in the field.

关键词： activation attempted IgE

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“Polymer-in-ceramic” based poly(ε-caprolactone)/ceramic composite electrolyte for all-solid-state batteries

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Journal of Energy chemistry 2021年第1期30卷 318-325,I0010页

作者： Bohao Zhang Yulong Liu Jia Liu Liqun Sun Lina cong Fang Fu alain Mauger christian M.Julien Haiming Xie Xiumei Pan National&Local United Engineering Laboratory for Power Battery Department of ChemistryNortheast Normal UniversityChangchun 130024JilinChina Institut de Minéralogie de Physique des Matériaux et de Cosmochimie(IMPMC)Sorbonne UniversitéCNRS UMR 75904 Place Jussieu75005 ParisFrance

Inspired by the concept of "polymer-in-ceramic",a composite poly(ε-caprolactone)(PcL)/ceramic containing LiTFSI is prepared and investigated as a solid electrolyte for all-solid-state *** composite with the optimum concentration of 45 wt% LiTFSI and 75 wt% Li1.5Al0.5Ge1.5(PO4)3(LAGP,NASIcON-type structure) exhibits a high ionic conductivity(σi=0.17 mS cm-1) at 30℃,a transference number of 0.30,and is stable up to 5.0 *** composite electrolyte is a flexible and self-standing ***-state LiFePO4//Li batteries with this composite electrolyte demonstrate excellent cycling stability with high discharge capacity of 157 mA h g-1,high capacity retention of 96% and coulombic efficiency of 98.5% after 130 cycles at 30℃ and 0.1 c *** electrochemical properties are better than other PcL-based allsolid-lithium batteries,and validate the concept of "polymer-in-ceramic" by avoiding the drawback of lower conductivity in prior "polymer-in-ceramic" electrolyte at high concentration of the ceramic.

关键词： All-solid-state electrolyte Polymer-in-ceramic Poly(ε-caprolactone)/LAGP composite High fluorinated SEI layer

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便携式微型发射光谱仪在金属工业中的应用(英文)

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冶金分析 2007年第5期27卷 8-13页

作者： TUSSET Victor LEMAIRE Olivier ZEIMETZ Eva DUPONT alain c.R.M. LiègeBelgium

通过有证参考物质对便携式在线发射光谱仪在不同金属类别的快速成分控制的应用进行了考察。分析了大量的不同样品(低碳和超低碳钢,不锈钢,铝、镍和铜基样品),并通过采用参考的化学分析方法如IcP-OES,XRF,c和S的燃烧等等方法对结果进行... 详细信息

通过有证参考物质对便携式在线发射光谱仪在不同金属类别的快速成分控制的应用进行了考察。分析了大量的不同样品(低碳和超低碳钢,不锈钢,铝、镍和铜基样品),并通过采用参考的化学分析方法如IcP-OES,XRF,c和S的燃烧等等方法对结果进行了评价。对于低碳钢中的c,S和P的分析,通过特别的校正方法,采用新型的便携式光谱仪能获得与传统的固定式光谱仪相当的分析效果。本文也列举了多种非进样直接分析的相关应用实例。

关键词：便携式在线发射光谱仪快速成分控制金属

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cr^(3+):LiNbO_3晶体生长和光谱特性的研究

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无机材料学报 2005年第4期20卷 875-882页

作者：游振宇涂朝阳朱昭捷李坚富 alain Brenier 中国科学院福建物质结构研究所 Laboratoire de Physico-chimie des Matériaux Luminescents UMR CNRS 5620Université Claude Bernard-Lyon110 rue Ampère69622 VilleurbanneFrance

采用提拉法从近化学计量比的熔体中生长出尺寸为φ20mm×60mm的优质LiNbO3:cr (cLN)晶体,其光学均匀度为7.05E-005.进行了吸收和荧光光谱的测定研究.吸收谱测试表明:cr3+离子在晶体中有两个宽且强的吸收带及两个微弱的吸收线,两宽带中... 详细信息

采用提拉法从近化学计量比的熔体中生长出尺寸为φ20mm×60mm的优质LiNbO3:cr (cLN)晶体,其光学均匀度为7.05E-005.进行了吸收和荧光光谱的测定研究.吸收谱测试表明:cr3+离子在晶体中有两个宽且强的吸收带及两个微弱的吸收线,两宽带中心波长分别为481和657nm,对应于4A2→4T1和4A2→4T2两个具有相同的总自旋能级之间的跃迁,在4A2→4T2吸收宽带的长波边缘处有个很小的吸收峰,其波长为727nm,对应于4A2→2E(R 线)的跃迁.荧光测试表明:当激发波长为660nm时,cLN晶体荧光宽带和一个较弱的荧光线峰并存,宽带范围为800—982nm,峰值波长为870nm,对应于4T2→2E,4A2的联合能级跃迁,荧光线峰波长约为752nm,其强度较弱,相应于2E→4A2(零声子线)能级跃迁.计算了晶场强度和Racah参数,其Dq,B,c大小分别为1522.1、542.5和3218.7cm-1,Dq/B=2.81, 晶体属于强场介质.研究表明,cLN晶体具备可调谐激光晶体的基本光谱要求,且有良好的物化性能,可以实现宽频带可调谐激光输出.又具有较大的倍频系数,有望实现410nm附近紫外的自倍频激光输出.

关键词： LiNbO3:cr(cLN)晶体晶体生长光谱特性

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ART ET DELIRE DANS ILLUMINATIONS

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法国研究 1989年第2期 32-39页

作者： yves-alain FAVRE

Eblouissante et irritante énigme, Illuminations apparait d’emblée comme une oeuvre paradoxale. On y apercoit d’abord une explosion d’images incontrolées qui naissent au cours de la reverie et qui surgissent, s’enchainent et s’effacent selon des processus qui échappent à toute logique. Mais on y décèle aussi toute une organisatiou et toute une concertation qui président à ces tableaux visionnaires. Le poète manifeste une volonté d’art, en intervenant dans la mise en scène du speeta-

关键词：切落主任

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Glutamate dehydrogenase and Na^+-K^+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

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chinese Journal of Oceanology and Limnology 2011年第2期29卷 343-349页

作者：李二超 Leticia ARENA Gabriel LIZAMA Gabriela GAXIOLA Gerard cUZON carlos ROSAS 陈立侨 alain VAN WORMHOUDT School of Life Science East China Normal University UMR5178 Station de Biologie Marine du Musum National d'Histoire NaturelleBP225Concarneau 29900France Unidad Multidisciplinaria de Docencia e Investigación Facultad de CienciasUniversidad Nacional Autónoma de MxicoPuerto de abrigo s/nSisal C.P.97350HunucmYucatnMexico IFREMER TahitiFrench Polynesia

Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp *** effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(***) were *** involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and *** experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of *** 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein ***,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and *** fed with 40% protein at 25 in salinity(optimal conditions) were used as a *** of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the *** fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 *** salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 *** findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein *** dietary protein level could improve the osmoregulation capacity of *** with more energy resources allocated to GDH activity and expression.

关键词： Litopenaeus vannamei gene expression glutamate dehydrogenase Na^+-K^+ ATPase protein salinity

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Detection of Burned Areas through Spectral Indices Analysis of Sentinel-2A Satellite Images in the Abokouamékro Wildlife Reserve (central, côte d’Ivoire)

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Open Journal of Applied Sciences（应用科学） 2024年第1期

作者： Bob Kouakou Kouadio Sié Ouattara alain clément Jean-Marc Gala Bi Zaouri Jean-Luc Kouadio Kouassi Jean-Luc Edouard Kouakou N’guessan

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Real-Time Thermomechanical Modeling of PV cell Fabrication via a POD-Trained RBF Interpolation Network

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computer Modeling in Engineering & Sciences 2020年第3期122卷 757-777页

作者： Arka Das Anthony Khoury Eduardo Divo Victor Huayamave Andresceballos Ron Eaglin alain Kassab Adam Payne Vijay Yelundur Hubert Seigneur Embry-Riddle Aeronautical University Department of Mechanical Engineering1 Aerospace Blvd.Daytona BeachFL 32114USA. central Technological corporation LongwoodFL 32779USA. University of central Florida Department of Mechanical and Aerospace Engineering4000 Central Florida Blvd.OrlandoFL 32816USA. Suniva Inc. 5765 Peachtree Industrial Blvd.NorcrossGA 30092USA. Florida Solar Energy center University of Central FloridaCocoa BeachFL 32922USA.

This paper presents a numerical reduced order model framework to simulate the physics of the thermomechanical processes that occur during c-Si photovoltaic(PV)cell fabrication.A response surface based on a radial basis function(RBF)interpolation network trained by a Proper Orthogonal Decomposition(POD)of the solution fields is developed for fast and accurate approximations of thermal loading conditions on PV cells during the fabrication *** outcome is a stand-alone computational tool that provides,in real time,the quantitative and qualitative thermomechanical response as a function of user-controlled input parameters for fabrication processes with the precision of 3D finite element analysis(FEA).This tool provides an efficient and effective avenue for design and optimization as well as for failure prediction of PV cells.

关键词： Silicon wafers photovoltaic cell contact firing proper orthogonal decomposition radial basis functions.

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雪铁龙有理由相信——cITROEN c4 WRc

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世界汽车 2007年第3期 54-57页

作者： alain

从蒙特卡罗站开始，雪铁龙c4 WRc正式接替了2006款赛纳WRc参加2007赛季的世界汽车拉力锦标赛，这也表明了雪铁龙公司准备长期投入世界拉力锦标赛的态度。

关键词：雪铁龙公司蒙特卡罗汽车拉力锦标赛动力系统

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