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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji Umezawa Osamu Tsuzukibashi akira Fukatsu Mana Fuchigami Chiaki Komine Hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato Nomoto Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

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Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer

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World Journal of Gastroenterology 2010年第15期16卷 1854-1858页

作者： Minoru Tomizawa Fuminobu shinozaki Takao Sugiyama Shigenori Yamamoto Makoto Sueishi Takanobu Yoshida Department of Gastroenterology National Hospital Organization Shimoshizu Hospital Department of Radiology National Hospital Organization Shimoshizu Hospital Department of Rheu-matology National Hospital Organization Shimoshizu Hospital Department of Pediatrics National Hospital Organization Shimoshizu Hospital Department of Internal Medicine National Hospital Organization Shimoshizu Hospital

AIM:To develop a molecular therapy for pancreatic cancer, the insulin-like growth factor-I (IGF-I) signaling pathway was ***: Pancreatic cancer cell lines (MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4) were cultured in media with 10 mL/L fetal bovine serum. Western blotting analysis was performed to clarify the expression of IGF-I receptor (IGF-IR). Picropodophyllin (PPP), a specific inhibitor of IGF-IR, LY294002, a specific inhibitor of phosphatidylinositol3 kinase (PI3K), and PD98059, a specific inhibitor of mitogen-activated protein kinase, were added to the media. After 72 h, a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay was performed to analyze cell proliferation. A wound assay was performed to analyze cell motility with hematoxylin and eosin (HE) staining 48 h after addition of each inhibitor. RESULTS: All cell lines clearly expressed not only IGF-IR but also phosphorylated IGF-IR. PPP significantly suppressed proliferation of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 36.9% ± 2.4% (mean ± SD), 30.9% ± 5.5%, 23.8% ± 3.9%, 37.1% ± 5.3%, 10.4% ± 4.5%, 52.5% ± 4.5% and 22.6% ± 0.4%, at 2 μmol/L, respectively (P < 0.05). LY294002 significantly suppressed proliferation of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 44.4% ± 7.6%, 32.9% ± 8.2%, 53.9% ± 8.0%, 52.8% ± 4.0%, 32.3% ± 4.2%, 51.8% ± 4.5%, and 30.6% ± 9.4%, at 50 μmol/L, respectively (P < 0.05). PD98059 did not significantly suppress cell proliferation. PPP at 2 μmol/L suppressed motility of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 3.0% ± 0.2%, 0%, 0%, 2.0% ± 0.1%, 5.0% ± 0.2%, 3.0% ± 0.1%, and 5.0% ± 0.2%, respectively (P < 0.05). LY294002 at 50 μmol/L suppressed motility of MIA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 to 3.0% ± 0.2%, 0%, 3.0% ± 0.2%, 0%, 0%, 0% and 3% ± 0.1%, respectively (P < 0.05). PD980509 at 20 μmol/L did not suppress

关键词： Insulin-like growth factor-I receptor Phosphatidylinositol 3 kinase Pancreatic neoplasms

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Petrological study of the western Iratsu mass from the Sambagawa metamorphic belt, central Shikoku, Japan

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2005年第7期6卷 649-655页

作者：厉子龙 TAKASU akira Department of Earth Science Zhejiang University Department of Earth Science Shimane University

The western Iratsu mass, the largest tectonic body in the Sambagawa metamorphic belt, central Shikoku, is mainly composed of epidote amphibolite with minor amounts of eclogite. Systematically, a majority of garnets show bell-shaped chemical zoning of pyrope contents and Mg/(Mg+Fe2+) monotonously increasing outward. The grossular component in zonal garnet increases outwards, maximizes at an intermediate part, and then decreases towards the outermost rim, reflecting a process from increasing to decreasing pressure conditions during the prograde metamorphism. Jadeite contents of omphacite range from 25～20mole% within the cores to 15～10 mole% at the rims, implying a pressure-decreasing process (from 11 × 105 Pa to 8 × 105 Pa). The peak pressure-temperature (P-T) condition of 630～680 ℃ and ca. 15× 105 Pa in the western Iratsu mass is much higher than that of (610±25) ℃ and (10± 1)× 105 Pa of the Sambagawa oligoclase-biotite zone schists. The authors suggest a clockwise P-T-t path for the western Iratsu mass.

关键词： Petrology P-T-t path Western Iratsu mass Sambagawa belt

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HsfAld, a Protein Identified via FOX Hunting Using Thellungiella salsuginea cDNAs Improves Heat Tolerance by Regulating Heat-Stress-Responsive Gene Expression

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Molecular Plant 2013年第2期6卷 411-422页

作者： Yukari Higashi Naohiko Ohama Tomoko Ishikawa Taku Katori Ayaka Shimura Kazuya Kusakabe Kazuko Yamaguchi-shinozaki Junko Ishida Maho Tanaka Motoaki Seki Kazuo shinozaki Yoichi Sakata Takahisa Hayashi Teruaki Taji Department of Bio-Science Tokyo University of Agriculture 1-1-1 Sakuragaoka Setagaya Tokyo 156-8502 Japan Graduate School of Agriculture and Life Sciences University of Tokyo Tokyo 113-8657 Japan RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsururni-ku Yokohama 230-0045 Japan

Thellungiella salsuginea （formerly T. halophila）, a species closely related to Arabidopsis （Arabidopsis thali-ana）, is tolerant not only to high salt levels, but also to chilling, freezing, and ozone. Here, we report that T. salsuginea also shows greater heat tolerance than Arabidopsis. We identified T. salsuginea HsfAld （TsHsfAld） as a gene that can confer marked heat tolerance on Arabidopsis. TsHsfAld was identified via Full-length cDNA Over-eXpressing gene （FOX） hunt-ing from among a collection of heat-stress-related T. salsuginea cDNAs. Transgenic Arabidopsis overexpressing TsHsfAld showed constitutive up-regulation of many genes in the Arabidopsis AtHsfA1 regulon under normal growth tempera-ture. in Arabidopsis mesophyll protoplasts, TsHsfAld was localized in both the nucleus and the cytoplasm. TsHsfAld also interacted with AtHSP90, which negatively regulates AtHsfAls by forming HsfA1-HSP90 complexes in the cytoplasm. It is likely that the partial nuclear localization of TsHsfAld induced the expression of the AtHsfAld regulon in the transgenic plants at normal temperature. We also discovered that transgenic Arabidopsis plants overexpressing AtHsfAldwere more heat-tolerant than wild-type plants and up-regulated the expression of the HsfAld regulon, as was observed in TsHsfAld-overexpressing plants. We propose that the products of both TsHsfAld and AtHsfAld function as positive regulators of Arabidopsis heat-stress response and would be useful for the improvement of heat-stress tolerance in other plants.

关键词： Thellungiella salsuginea heat-stress tolerance HsfA1 FOX hunting.

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Pediatric interfacility transport effects on mortality and length of stay

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World Journal of Pediatrics 2021年第4期17卷 400-408页

作者： Rod M.shinozaki Andreas Schwingshackl Neeraj Srivastava Tristan Grogan Robert B.Kelly David Geffen School of Medicine at UCLA Los AngelesCAUSA University of California IrvineIrvineCAUSA Children’s Hospital of Orange County 1201 West La Veta AvenueOrangeCA92868USA

Background We aimed to evaluate the effects of interfacility pediatric critical care transport response time,physician presence during transport,and mode of transport on mortality and length of stay(LOS)among pediatric *** hypothesized that a shorter response time and helicopter transports,but not physician presence,are associated with lower mortality and a shorter *** Retrospective,single-center,cohort study of 841 patients(<19 years)transported to a quaternary pediatric intensive care unit and cardiovascular intensive care unit between 2014 and 2018 utilizing patient charts and transport *** linear and logistic regression analyses adjusted for age,diagnosis,mode of transport,response time,stabilization time,return duration,mortality risk(pediatric index of mortality-2 and pediatric risk of mortality-3),and inotrope,vasopressor,or mechanical ventilation presence on *** Four hundred and twenty-eight(50.9%)patients were transported by helicopter,and 413(49.1%)were transported by *** accompanied 239(28.4%)*** median response time was 2.0(interquartile range 1.4–2.9)*** physician presence increased the median response time by 0.26 hours(P=0.020),neither physician presence nor response time significantly affected mortality,ICU length of stay(ILOS)or hospital length of stay(HLOS).Helicopter transports were not significantly associated with mortality or ILOS,but were associated with a longer HLOS(3.24 days,95%confidence interval 0.59–5.90)than ambulance transports(P=0.017).Conclusions These results suggest response time and physician presence do not significantly affect mortality or *** may reflect the quality of pre-transport care and medical control *** transports were only associated with a longer *** analysis provides a framework for examining transport workforce needs and associated costs.

关键词： Helicopter Hospital length of stay Pediatric critical care Pediatric intensive care unit Transport medicine

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Slow and fast light in quantum-well and quantum-dot semiconductor optical amplifiers Invited Paper

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Chinese Optics Letters 2008年第10期6卷 736-742页

作者： Piotr Konrad Kondratko akira Matsudaira Department of Electrical and Computer Engineering University of Illinois at Urbana-ChampaignUrbana IL61801USA

Slow and fast light in quantum-well （QW） and quantum-dot （QD） semiconductor optical amplifiers （SOAs） using nonlinear quantum optical effects are presented. We demonstrate electrical and optical controls of fast light using the coherent population oscillation （CPO） and four wave mixing （FWM） in the gain regime of QW SOAs. We then consider the dependence on the wavelength and modal gain of the pump in QW SOAs. To enhance the tunable photonic delay of a single QW SOA, we explore a serial cascade of multiple amplifiers. A model for the number of QW SOAs in series with variable optical attenuation is developed and matched to the experimental data. We demonstrate the scaling law and the bandwidth control by using the serial cascade of multiple QW SOAs. Experimentally, we achieve a phase change of 160^o and a scaling factor of four at 1 GHz using the cascade of four QW SOAs. Finally, we investigate CPO and FWM slow and fast light of QD SOAs. The experiment shows that the bandwidth of the time delay as a function of the modulation frequency changes in the absorption and gain regimes due to the carrier-lifetime variation. The tunable phase shift in QD SOA is compared between the ground- and first excited-state transitions with different modal gains.

关键词： SOA CPO Slow and fast light in quantum-well and quantum-dot semiconductor optical amplifiers Invited Paper FWM well

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Robust Stability of Nonlinear Plants with a Non-symmetric Prandtl-Ishlinskii Hysteresis Model

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International Journal of Automation and computing 2010年第2期7卷 213-218页

作者： akira Inoue Graduate School of Natural Science and Technology Okayama University

In this paper, robust stability of nonlinear plants represented by non-symmetric Prandtl-Ishlinskii （PI） hysteresis model is studied. In general, PI hysteresis model is the weighted superposition of play or stop hysteresis operators, and the slopes of the operators are considered to be the same. In order to make a hysteresis model, a modified form of non-symmetric play hysteresis operator with unknown slopes is given. The hysteresis model is described by a generalized Lipschitz operator term and a bounded parasitic term. Since the generalized Lipschitz operator is unknown, a new condition using robust right coprime factorization is proposed to guarantee robust stability of the controlled plant with the hysteresis nonlinearity. As a result, based on the proposed robust condition, a stabilized plant is obtained. A numerical example is presented to validate the effectiveness of the proposed method.

关键词： Prandtl-Ishlinskii (PI) hysteresis model robust stability right coprime factorization non-symmetric play hysteresis operator nonlinear plants.

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Photocatalytic dry reforming of methane by rhodium supported monoclinic TiO_(2)-B nanobelts

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Journal of Energy Chemistry 2022年第8期31卷 562-571,I0015页

作者： Masaru Kushida akira Yamaguchi Masahiro Miyauchi School of Materials and Chemical Technology Tokyo Institute of Technology2-12-1 OokayamaMeguro-kuTokyo 152-8552Japan

The conversion of methane and carbon dioxide into syngas(dry reforming of methane;DRM)has attracted attention owing to the potential to reuse greenhouse *** dioxide(TiO_(2))-based photocatalysts,which have been widely commercialized owing to their high efficiency,non-toxicity,and low cost,are strongly desired in ***,we report a monoclinic-phase TiO_(2)-B nanobelts-supported rhodium(Rh/TiO_(2)-B nanobelts)catalyst that efficiently promotes DRM under ultraviolet light irradiation at low *** holes in the TiO_(2)-B nanobelts were used to oxidize methane,while the electrons were trapped in rhodium to reduce carbon ***/TiO_(2)-B nanobelts exhibited considerably higher durability and activity than Rh-loaded conventional TiO_(2)(anatase and rutile),owing to the lattice and/or surface oxygen reactivity in TiO_(2)-B nanobelts,which was suggested by X-ray photoelectron spectroscopy measurements and photocatalytic performance tests under an atmosphere of methane *** study paves the path for the effective utilization of methane by constructing active TiO_(2)-based nanometal photocatalysts.

关键词： Photocatalysis Dry reforming of methane Greenhouse gas conversion Gas-phase reaction Titanium dioxide TiO_(2)-B

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强震损伤格栅墙处理地基抗液化性能离心模型试验研究

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岩土工程学报 2024年第S1期46卷 11-15页

作者：周燕国曹渊石川明陈云敏浙江大学软弱土与环境土工教育部重点实验室浙江大学岩土工程研究所浙江大学超重力研究中心浙江杭州310058 清水建设技术研究所日本东京135-8530

水泥土格栅墙能有效减缓被围束土体在地震作用下的剪切荷载及变形,已广泛应用为一种高效的抗液化对策。针对其长期服役过程,开展了两组超重力离心模型试验以研究强振历史对水泥土格栅墙内被围束土体液化响应的影响。两组模型均由15 m厚... 详细信息

水泥土格栅墙能有效减缓被围束土体在地震作用下的剪切荷载及变形,已广泛应用为一种高效的抗液化对策。针对其长期服役过程,开展了两组超重力离心模型试验以研究强振历史对水泥土格栅墙内被围束土体液化响应的影响。两组模型均由15 m厚的易液化含黏粒砂土和2.5 m厚的粗砂层组成。其中,一组模型采用水泥土格栅墙对易液化层进行加固;另一组模型则未进行处理,作为自由场地对照组。两组模型均经历振幅为0.15g的振动事件,且在0.15g振动事件前,对两组模型施加振幅为0.4g的振动事件作为强振历史。试验结果表明,格栅墙在强振下主要的损伤模式表现为竖向贯穿型裂缝,并部分伴随有局部斜向裂缝,且外侧格室对于中心格室表现出类似于群桩基础的“遮蔽效应”。强振历史后,格栅墙中心格室内被围束土体的超静孔压显著低于自由场地,损伤后格栅墙仍具有良好的抗液化效果。同时,格栅墙由于强振后整体刚度的削弱,其与下卧粗砂层间的剪切变形显著减小。

关键词：地震液化水泥土格栅墙地基处理离心机模型试验地震历史

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Surface Texturing of TiO_2 Film by Mist Deposition of TiO_2 Nanoparticles

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Nano-Micro Letters 2013年第2期5卷 129-134页

作者： Gang Qin akira Watanabe Institute of Multidisciplinary Research for Advanced Materials Tohoku University School of Materials Science and Engineering Henan Polytechnic University

Unique and various microstructures of titanium oxide(TiO_2 ) film including macroporous structure, chromatic veins and rings, have been easily fabricated by mist deposition method on silicon substrate with mild preparation conditions. Rutile phase TiO_2 nanoparticles were directly used as starting material to prepare film and led to a simple preparation process. It was found that several different microstructures existed in the sample and changed with the varied positions from the center to the edge of the film when the concentration of the TiO_2 suspension is 0.06 mol/l, the deposition time is 30 min, the flow rate is 1 l/min and the temperature is150. The surface texturing shows apparent distinction as the concentration of the TiO_2 suspension decreased to 0.03 mol/l and 0.01 mol/l.

关键词： Mist deposition TiO2 film TiO2 nanoparticle Surface texturing Microstructure

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