HsfAld, a Protein Identified via FOX Hunting Using Thellungiella salsuginea cDNAs Improves Heat Tolerance by Regulating Heat-Stress-Responsive Gene Expression

作     者：Yukari Higashi Naohiko Ohama Tomoko Ishikawa Taku Katori Ayaka Shimura Kazuya Kusakabe Kazuko Yamaguchi-Shinozaki Junko Ishida Maho Tanaka Motoaki Seki Kazuo Shinozaki Yoichi Sakata Takahisa Hayashi Teruaki Taji 

作者机构：Department of Bio-Science Tokyo University of Agriculture 1-1-1 Sakuragaoka Setagaya Tokyo 156-8502 Japan Graduate School of Agriculture and Life Sciences University of Tokyo Tokyo 113-8657 Japan RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsururni-ku Yokohama 230-0045 Japan 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第2期

页      面：411-422页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 071010[理学-生物化学与分子生物学] 07[理学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by a Grant-in-Aid for Scientific Research on Innovative Areas(to T.T.) supported by for Scientific Research (C)(to T.T.) supported by from the Ministry of Education, Culture, Sports, Science and Technology of Japan 

主　　题：Thellungiella salsuginea heat-stress tolerance HsfA1 FOX hunting. 

摘      要：Thellungiella salsuginea （formerly T. halophila）, a species closely related to Arabidopsis （Arabidopsis thali-ana）, is tolerant not only to high salt levels, but also to chilling, freezing, and ozone. Here, we report that T. salsuginea also shows greater heat tolerance than Arabidopsis. We identified T. salsuginea HsfAld （TsHsfAld） as a gene that can confer marked heat tolerance on Arabidopsis. TsHsfAld was identified via Full-length cDNA Over-eXpressing gene （FOX） hunt-ing from among a collection of heat-stress-related T. salsuginea cDNAs. Transgenic Arabidopsis overexpressing TsHsfAld showed constitutive up-regulation of many genes in the Arabidopsis AtHsfA1 regulon under normal growth tempera-ture. in Arabidopsis mesophyll protoplasts, TsHsfAld was localized in both the nucleus and the cytoplasm. TsHsfAld also interacted with AtHSP90, which negatively regulates AtHsfAls by forming HsfA1-HSP90 complexes in the cytoplasm. It is likely that the partial nuclear localization of TsHsfAld induced the expression of the AtHsfAld regulon in the transgenic plants at normal temperature. We also discovered that transgenic Arabidopsis plants overexpressing AtHsfAldwere more heat-tolerant than wild-type plants and up-regulated the expression of the HsfAld regulon, as was observed in TsHsfAld-overexpressing plants. We propose that the products of both TsHsfAld and AtHsfAld function as positive regulators of Arabidopsis heat-stress response and would be useful for the improvement of heat-stress tolerance in other plants.