检索结果-南通市图书馆

Immunomodulatory activity of a lupane triterpenoid ester isolated from the eastern Nigeria mistletoe,Loranthus micranthus(Linn)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 514-522页

作者： Omeje Edwin Ogechukwu Osadebe Patience Ogoamaka Nworu Chukwuemeka Sylvester akira Kawamura Peter Proksch Department of Pharmaceutical and Medicinal Chemistry Faculty of Pharmaceutical SciencesUniversity of Nigeria410001NsukkaNigeria Department of Pharmacology and Toxicology Faculty of Pharmaceutical SciencesUniversity of Nigeria410001NsukkaNigeria Department of Chemistry Hunter College of CUNYThe City University of New YorkU.S.A Department of Pharmaceutical Biology Heinrich-Hein UniversityDusseldorfGermany

Objective:To provide further evidence for the ethnomedicinal use of the Eastern Nigeria mistletoe,Loranthus micranthus(***),as an ***:Solvent fractions from the crude extract of the mistletoe plant was obtained and screened by the cell mediated delayed type hypersensitivity reaction(DTHR) model in *** the immunomodulatory potentials of a major lupane triterpenoid ester isolated from an active hexane fraction of the Eastern Nigeria mistletoe was *** lupeol-based triterpenoid esters: 7β15α-dihydroxyl-lup-20(29)-ene-3β-palmitate(Ⅰ),7β,15α-dihydroxyl-lup-20(29)- ene-3β-stearate(Ⅱ) and 7β,15α-dihydroxyl-lup-20(29)-ene-3β-decadecanoate(Ⅲ) were isolated from the plant leaves epiphyting on a local kola nut tree and were characterized. Compound 1 was subjected to cell proliferation studies using C57H1/6 splenocvtes at three dose levels(5.25 and 100μg/mL) in presence of ***,the effect of this compound on IL-8 receptor expression was evaluated at three doses(1,5 and 10μg/mL) using the real time polymerase chain reaction ***:This triterpenoid ester produced some enhancement of the splenocytes at the tested doses but at doses higher than 5μg/mL caused inhibition of the IL-8 receptor ***:The present findings support the ethnomedicinal use of the Eastern Nigeria Mistletoe in the management of diseases affecting the immune *** trilerpenoid(s) have some immunomodulatory abilities on splenocytes and IL-8 receptors and may partly account lor the overall immunomodulatory activity of this plant.

关键词： Lupane triterpenoid Loranthus micranthus Eastern Nigeria mistletoe Interleukin-8 Cell proliferation Anti-inflammatory

Inverse correlation between reactive oxygen species in unwashed semen and sperm motion parameters as measured by a computer-assisted semen analyzer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2017年第3期19卷 350-354页

作者： Teppei Takeshima Yasushi Yumura Kengo Yasuda Hiroyuki Sanjo Shinnosuke Kuroda Hiroyuki Yamanaka akira Iwasaki Department of Urology Reproductive Center Yokohama City University Medical Center Yokohama Kanagawa Japan YMRAC Urology Office Yokohama Kanagawa Japan.

This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient＇s first consultation, semen parameters were measured using SMASTM or CellSoft 3000TM, and production of reactive oxygen species was measured using a computer-driven LKB Wallac Luminometer 1251 Analyzer. The patients were divided into two groups： reactive oxygen species - positive and negative. The semen parameters within each group were measured using one of the two computer-assisted semen analyzer systems and then compared. Correlations between reactive oxygen species levels and sperm motion parameters in semen from the reactive oxygen species - positive group were also investigated. Reactive oxygen species were detected in semen samples of 282 cases （33.3%）. Sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.01; P 〈 0.05）, and progressive motility （P 〈 0.01; P 〈 0.01） were markedly lower in the reactive oxygen species - positive group than in the reactive oxygen species - negative group. Among the sperm motion parameters in the reactive oxygen species - positive group, sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.05; P 〈 0.01）, mALH （P 〈 0.05; P 〈 0.01）, and progressive motility （P 〈 0.05; P 〈 0.01） also showed inverse correlations with the logarithmic transformed reactive oxygen species levels. Therefore, this study demonstrated that excessive reactive oxygen species in semen damage sperm concentration, motility, and other sperm motion parameters.

关键词： oxidative stress reactive oxygen species semen analysis sperm motility

GROWTH AND MORPHOLOGY OF POLYMER-ACTIN COMPLEXES

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2007年第1期25卷 47-55页

作者： Hyuck Joon Kwon Kazuhiro Shikinaka akira Kakugo Hidemitsu Furukawa Yoshihito Osada 龚剑萍 Graduate School of Science Hokkaido UniversitySapporo 060-0810Japan Graduate School of Science Hokkaido UniversitySapporo 060-0810Japan SORSTJSTSapporo 060-0810Japan

F-actins are semi-flexible polyelectrolytes and can be assembled into large polymer-actin complex with polymorphism through electrostatic interaction with polycations. This study investigates the structural phase behavior and the growth of polymer-actin complexes in terms of its longitudinal and lateral sizes. Our results show that formation of polymer-actin complexes is cooperative, and morphology and growth of polymer-actin complexes depend on polycation species and concentrations of polycation and salt in a constant actin concentration. We found that the longitudinal growth and lateral growth of polymer-actin complexes are dominated by different factors. This induces the structural polymorphism of polymer-actin complexes. Major factors to influence the polymorphism of polymer-actin complexes in polyelectrolytc system have been discussed. Our results indicate that the semi-flexible polyelectrolyte nature of F-actins is important for controlling the morphology and growth ofactin architectures in cell.

关键词： F-actin Polymer-actin complex Polycation concentration (Cp) KCl concentration (Cs) Fluorescence microscopy Transmission electron microscopy (TEM) Rod-like polyelectrolyte.

Nitrogen resorption and fractionation during leaf senescence in typical tree species in Japan

在线全文

学校读者我要写书评

暂无评论

Journal of Forestry Research 2020年第6期31卷 2053-2062页

作者： Ayako Enta Mika Hayashi Maximo Larry Lopez Caceres Lei Fujiyoshi Toshiro Yamanaka akira Oikawa Felix Seidel Faculty of Agriculture Yamagata UniversityWakabamachi 1-23Tsuruoka 997-8555Japan Research Institute for Humanity and Nature Motoyama 457-4KamigamoKita-kuKyoto 603-8047Japan Department of Ocean and Environmental Sciences Tokyo University of Marine Science and Technology4-5-7 Konan Minato-kuTokyo 108-8477Japan

In northeastern Japan,an area of high precipitation and mountains,beech(Fagus creanata Blume),larch(Larix kaempferi Lamb.),cedar(Cryptomeria japonica ***)and black locust(Robinia pseudoacacia L.)were evaluated for N resorption and N isotope fractionation in preand post-abscission leaves in comparison to green *** highest leaf N concentration in summer corresponded to the N-fi xing black locust,followed in decreasing order by the deciduous beech and larch and evergreen *** the other hand,the lowest N resorption effi ciency corresponded to black locust and the highest to beech,in increasing order by larch and *** tree species returned signifi cant amounts of N before leaf abscission;however,N isotope fractionation during leaf N resorption was only found for beech,with a depleted N isotope value from green to pre-abscission *** most N,however,was resorbed from pre-abscission to *** result may indicate thatδ15 N fractionation took place during N transformation processes,such as protein hydrolysis,when the concentration of free amino acids increased *** diff erence in the type of amino acid produced by each species could have infl uenced the N isotope ratio in beech but not in the other tree *** results of this study showed that it is possible to infer the type and timing of processes relevant to N resorption by analyzing leafδ15 N variation during senescence.

关键词： Amino acids Cool temperate forest Fresh litter N resorption δ15 N

Activities of nicotinic acetylcholine receptors modulate neurotransmission and synaptic architecture

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2014年第24期9卷 2128-2131页

作者： akira Oda Hidekazu Tanaka CNS Drug Discovery Unit Pharmaceutical Research Division Takeda Pharmaceutical Company Limited Laboratory of Pharmacology Department of Biomedical Sciences College of Life Sciences Ritsumeikan University

The cholinergic system is involved in a broad spectrum of brain function, and its failure has been implicated in Alzheimer＇s disease. Acetylcholine transduces signals through muscarinic and nicotinic acetylcholine receptors, both of which influence synaptic plasticity and cognition. However, the mechanisms that relate the rapid gating of nicotinic acetylcholine receptors to persistent changes in brain function have remained elusive. Recent evidence indicates that nicotinic acetylcholine receptors activities affect synaptic morphology and density, which result in persistent rearrangements of neural connectivity. Further investigations of the relationships between nicotinic acetylcholine receptors and rearrangements of neural circuitry in the central nervous system may help understand the pathogenesis of Alzheimer＇s disease.

关键词： cholinergic system nicotinic acetylcholine receptors (nAChRs) Alzheimer's disease (AD) synaptic transmission synaptic plasticity synaptic morphology dendritic spine remodeling cognition

Two-year clinical outcome of denosumab treatment alone and in combination with teriparatide in Japanese treatment-naive postmenopausal osteoporotic women

在线全文

学校读者我要写书评

暂无评论

Bone Research 2017年第2期5卷 152-158页

作者： Yukio Nakamura Takako Suzuki Mikio Kamimura Shota Ikegami Kohei Murakami Shigeharu Uchiyama akira Taguchi Hiroyuki Kato Department of Orthopedic Surgery Shinshu University School of Medicine Matsumoto Japan Department of Orthopedic Surgery Showa-Inan General Hospital Komagane Japan Center of Osteoporosis and Spinal Disorders Kamimura Orthopaedic Clinic Matsumoto Japan and Department of Oral and Maxillofacial Radiology Matsumoto Dental University Shiojiri Japan

This randomized prospective study aimed to evaluate the clinical outcome of denosumab treatment alone and in combination with teriparatide in treatment-naive postmenopausal Japanese female patients with osteoporosis. Thirty patients were randomly assigned to two groups:(1) denosumab group(denosumab alone, n=13); and(2) combination group(denosumab+teriparatide, n=17). Serum bone-specific alkaline phosphatase(BAP), serum tartrate-resistant acid phosphatase(TRACP)-5b, urinary cross-linked N-terminal telopeptides of type I collagen(NTX), and bone mineral density(BMD) of L1–4 lumbar vertebrae(L-BMD)and bilateral total hips(H-BMD) were determined at the first visit and at various time points up to 24 months post-treatment to determine percentage changes. Serum TRACP-5b and urinary NTX were equally suppressed in both groups and maintained at low levels, with slight increases at 12, 18 and 24 months. BAP was significantly decreased in both groups from 4 to 24 months, with significant differences between the groups at 4, 8 and 15 months(P<0.05). L-BMD was significantly increased at most time points in both groups, with a significant difference between the combination group and denosumab group at 24 months(17.2% increase versus 9.6% increase; P<0.05). There was no significant difference in H-BMD between the two groups, although the levels tended to be higher in the combination group than in the denosumab group(9.5% increase versus 5.6% increase). These findings suggest that denosumab+teriparatide combination therapy may represent an important treatment for primary osteoporotic patients at high risk of vertebral fracture.

关键词： teriparatide denosumab treatment

Growth and luminescence properties of Eu-doped HfO2/α-Al2O3 eutectic scintillator

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2016年第8期34卷 796-801页

作者： Kei Kamada Kosuke Hishinuma Shunsuke Kurosawa Akihiro Yamaji Yasuhiro Shoji Jan Pejchal Yuji Ohashi Yuui Yokota akira Yoshikawa New Industry Creation Hatchery Center Tohoku University6-6-10 AobaAramakiAoba-kuSendaiMiyagi980-8579Japan C&A Corporation T-Biz 6-6-40 AobaAramakiAoba-kuSendaiMiyagi 980-8579Japan Institute for Material Reseach Tohoku University2-1-1 Katahira Aoba-kuSendal Miyagi980-8577Japan Institute of Physics AS CR Cukrovarnicka 1016253 PragueCzech Republic

HfO2/a-A12O3 eutectics were grown by the micro-pulling down （μ-PD） method at the composition of 63.2 mol.% of-A1203, 35.512 mol.% HfO2, 0.92 mol.% Eu203 and 0.368 tool.% Y2O3. Rod phases with around 3-4 μm diameter were observed on the transverse cross-section. The phase-separated scintillator fibers （PSSFs） structure with fibers of around 3-4 μm diameter and 10-20 μm length was observed. The electron back scattering patterns indicated crystal orientation of [101] for monoclinic HfO2 rod phase and [0001] for μt-A1203 matrix in the transverse cross-section. Eu3＋ 4f-4f emission was observed at 550-750 nm.

关键词： eutectic scintillator micro-pulling down method rare earths

Development of FGF-2-loaded electrospun waterborne polyurethane fibrous membranes for bone regeneration

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2021年第1期8卷 34-42页

作者： Chi Zhang Jianxiong Wang Yujie Xie Li Wang Lishi Yang Jihua Yu akira Miyamoto Fuhua Sun Department of Rehabilitation The Afiliated Hospital of Southwest Medical UniversityLuzhou 646000PR.China Department of Oncology The filiated Hospital of Southwest Medical UniversityLuzhou 646000P.R.China Faculty of Rehabilitation Department of Physical TherapyKobe International UniversityKobeJapan

Guided bone regeneration(G BR)membrane has been used to improve functional outcomes for periodontal regener ***,few studies have focused on the biomimetic membrane mim-icking the vascularization of the periodontal *** study aimed to fabricate waterborne polyurethane(WPU)fibrous membranes loaJed fibroblast growth factor-2(FGF-2)via emulsion electrospinning,which can promote regeneration of periodontal tissue via the vascularization of the biomimetic GBR membrane.A biodegradable WPU was synthesized by using lysine and dime-thylpropionic acid as chain extenders according to the rule of green chemical synthesis *** WPU fibers with FGF-2 was fabricated via emulsion *** results confirmed that controlled properties of the fibrous membrane had been achieved with controlled degradation,suitable mech anical properties and sustained release of the *** immunohistochemical expression of angiogenic-related factors was positive,meaning that FGF-2 loaded in fibers can sig-nificantly promote cell vasculariz *** fiber scaffold loaded FGF-2 has the potential to be used as a functional GBR membrane to promote the formation of extraosseous blood vessels during periodontal repairing.

关键词： waterborne polyure thane fibroblast growth fa ctor-2 emulsion electrospinning vascularization GBR membrane

Hydrogen promotes the activation of Cu, Zn superoxide dismutase in a rat corneal alkali-burn model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2020年第8期13卷 1173-1179页

作者： Takeshi Arima Tsutomu Igarashi Masaaki Uchiyama Maika Kobayashi Ikuroh Ohsawa akira Shimizu Hiroshi Takahashi Department of Ophthalmology Nippon Medical SchoolTokyo1138602Japan Department of Analytic Human Pathology Nippon Medical SchoolTokyo1138602Japan Biological Process of Aging Tokyo Metropolitan Institute of GerontologyTokyo173-0015Japan

AIM: To investigate the effects of hydrogen(H2) on Cu, Zn superoxide dismutase(SOD1) activation in a rat model of corneal alkali burn. METHODS: In each rat, one cornea was subjected to alkali exposure. Physiological saline(saline group) or H2-dissolved saline(H2 group) was instilled continuously on the cornea for 5 min before and after alkali exposure. Inflammatory cells, neovascularization, and cytoplasmic SOD1 levels were evaluated immunohistochemically in enucleated eyes from both groups. Three-dimensional ultrastructural tissue changes in the eyes were analyzed using low-vacuum scanning electron ***: The numbers of both inflammatory and vascular endothelial cells were significantly reduced in the corneas of the H2 group(P<0.01). Furthermore, H2 treatment increased both cytoplasmic SOD1 levels(P<0.01) and activity in corneal epithelial cells(P<0.01). Notably, the SOD1 activity level in the H2 group was approximately 2.5-fold greater than that in the saline ***: H2 treatment suppresses inflammation and neovascularization in the injured cornea and indirectly suppresses oxidative insult to the cornea by upregulating the SOD1 enzyme protein level and activity.

关键词： hydrogen alkali burn Cu,Zn superoxide dismutase low-vacuum scanning electron microscopy rats