检索结果-南通市图书馆

Developmental quantitative genetic analysis of body weights and morphological traits in the turbot, Scophthalmus maximus

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2015年第2期34卷 55-62页

作者： WANG Xin'an ma aijun ma Deyou Yellow Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences/Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture/Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology

In order to elucidate the genetic mechanism of growth traits in turbot during ontogeny, developmental genetic analysis of the body weights, total lengths, standard lengths and body heights of turbots was conducted by mixed genetic models with additive-dominance effects, based on complete diallel crosses with four different strains of Scophthalmus maximus from Denmark, Norway, Britain, and France. Unconditional genetic analysis revealed that the unconditional additive effects for the four traits were more significant than unconditional dominance effects, meanwhile, the alternative expressions were also observed between the additive and dominant effects for body weights, total lengths and standard lengths. Conditional analysis showed that the developmental periods with active gene expression for body weights, total lengths, standard lengths and body heights were 15–18, 15 and 21–24, 15 and 24, and 21 and 27 months of age, respectively. The proportions of unconditional/conditional variances indicated that the narrow-sense heritabilities of body weights, total lengths and standard lengths were all increased systematically. The accumulative effects of genes controlling the four quantitative traits were mainly additive effects, suggesting that the selection is more efficient for the genetic improvement of turbots. The conditional genetic procedure is a useful tool to understand the expression of genes controlling developmental quantitative traits at a specific developmental period（t-1→t） during ontogeny. It is also important to determine the appropriate developmental period（t-1→t） for trait measurement in developmental quantitative genetic analysis in fish.

关键词： Turbot(Scophthalmus maximus L.) growth developmental genetics genetic effect

Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2020年第1期38卷 294-305页

作者： SHANG Xiaomei ma aijun WANG Xin’an XIA Dandan ZHUANG Jiao Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShandong Key Laboratory of Marine Fisheries Biotechnology and Genetic BreedingQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyQingdao 266071China Laboratory for marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China College of marine and Environmental Sciences Tianjin University of Science and TechnologyTianjin 300457China

The transient receptor potential vanilloid 4(TRPV4),another Ca^2+entry channel,belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in ***,our understanding of the TRPV4 in fish remains ***,we studied the TRPV4 gene from Cynoglossus semilaevis,named CsTRPV4 that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4,including the domains of ANK repeats,six TM,TRP domain,and *** CsTRPV4 was expressed ubiquitously in examined tissues:higher expression in the heart,spleen,testis,and eye,but lower expression in kidney and ***,the expression of CsTRPV4 in lateral line was significantly higher than in many other tissues as the CsTRPV4 was expressed significantly in the free *** addition,CsTRPV4 in the free neuromast from the larval fish was significantly expressed in the hair cells of the free ***,the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in ***,which lays a foundation for further study of the functions of the free neuromasts.

关键词： transient receptor potential vanilloid 4 (TRPV4) Cynoglossus semilaevis gene expression free neuromasts in situ hybridization

Genetic parameters estimation for growth traits in cultured tiger pufferfish(fugu),Takifugu rubripes

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2022年第12期41卷 73-79页

作者： Xin’an Wang aijun ma Zhifeng Liu Zhibin Sun Liguang Zhu Haowen Chang Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China Key Laboratory of Sustainable Development of marine Fisheries Ministry of Agriculture and Rural AffairsQingdao 266071China Qingdao Key Laboratory for marine Fish Breeding and Biotechnology Qingdao 266071China Laboratory for marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China

The aim of this study was to evaluate the genetic parameters of the growth performance of Takifugu *** and genetic correlations were estimated for body weight(BW),body length(BL),total length(TL),chest measurement(CM)and trunk length(TKL)of *** from measurements of progeny at 6 months and 12 *** results showed that the heritability was 0.37 for BW6,0.19 for BL6,0.35 for TL6,0.29 for CM6,0.26 for TKL6,0.36 for BW12,0.26 for BL12,0.25 for TL12,0.31 for CM12 and 0.15 for *** range of genetic correlations estimated at 6 months was 0.025–0.725 and−0.002–0.706 at 12 *** results indicated that genetic improvement for faster growth rate or increased body weight in cultured *** was *** on selection theory,the selection strategy for traits with medium heritability is *** that these growth traits do not reach the high level of heritability,family selection should be *** positive genetic correlations among BW,BL,TL,CM,and TKL at 6 months,the five traits could be improved simultaneously through selective *** there was high genetic correlation only between BW12,BL12 and TL12,and negative correlations between TKL12 and BL12 as well as between CM12 and TL12,and only BW,BL and TL at 12 months could be improved simultaneously.

关键词： family genetic evaluation heritability genetic correlation

Estimation of genetic parameters for upper thermal tolerance and growth-related traits in turbot Scophthalmus maximus using the Bayesian method based on Gibbs sampling

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2018年第6期37卷 40-46页

作者： ma aijun WANG Xin'an HUANG Zhihui LIU Zhifeng CUI Wenxiao QU Jiangbo Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of marine Fisheries Ministry of Agriculture Qingdao Key Laboratory for marine Fish Breeding and Biotechnology Qingdao 266071 China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Yantai Tianyuan Aquatic Limited Corporation Yantai 264003 China

In order to carry out the genetic improvement of turbot upper thermal tolerance, it is necessary to estimate the genetic parameters of UTT（upper thermal tolerance） and growth-related traits. The objective of this study was to estimate genetic parameters for BW（body weight） and UTT in a two-generational turbot（Scophthalmus maximus L.） pedigree derived from four imported turbot stocks（England, France, Denmark and Norway）. A total of 42 families including 20 families from G1 generation and 22 families from G2 generation were used to test upper thermal tolerance（40–50 animals per family） in this study and the body weight of individuals were measured. The heritability of BW and UTT and the correlation between these two traits were estimated based on an individual animal model using Bayesian method based on two types of animal models with and without maternal *** results showed that the heritabilities for BW and UTT and phenotypic and genetic correlations between the two traits estimated from model without maternal effects were 0.239±0.141, 0.111±0.080, 0.075±0.026 and–0.019±0.011, respectively. The corresponding values from model with maternal effects were 0.203±0.115,0.055±0.026, 0.047±0.034 and –0.024±0.028, respectively. The maternal effects of BW and UTT were 0.050±0.017 and 0.013±0.004, respectively. The maternal effects had a certain influence on the genetic evaluation of the two traits. The findings of this paper provided the necessary background to determine the best selection strategy to be adopted in the genetic improvement program.

关键词： heritability genetic correlation thermotolerance body weight Turbot Bayesian inference

Estimation of genetic parameters for upper thermal tolerances and growth-related traits in turbot Scophthalmus maximus

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2019年第5期37卷 1736-1745页

作者： WANG Xin′an ma aijun HUANG Zhihui SUN Zhibin CUI Wenxiao QU Jiangbo YU Hong Shandong Key Laboratory of marine Fisheries Biotechnology and Genetic Breeding Qingdao Key Laboratory for Marine Fish Breeding and BiotechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Yantai Tianyuan Aquatic Limited Corporation Yantai 264006China

An abnormally high temperature produces a stress response in turbot causing large economic losses in the turbot aquaculture industry of China. A genetic improvement of the upper thermal tolerance (UTT) of turbot could allow cultured fi sh to adapt. A genetic evaluation of UTT is required for determining the practicability of including this trait into a breeding program. In this study, data were recorded from a temperature tolerance test conducted on 3 200 individual turbots from 32 full-sib groups. A cross-sectional linear model and a cross-sectional threshold probit model were used to analyze the test-period survival and a cross-sectional threshold logit model was used to analyze the test-day survival. In addition, phenotypic and genetic correlations between body weight and survival data were estimated. The estimated heritability values obtained from the cross-sectional linear model (CSL), the cross-sectional threshold (probit) model (THRp), and the cross-sectional threshold (logit) model (THRl) were 0.247 9±0.108 3, 0.288 3±0.161 2, and 0.106 9±0.045 2, respectively. The correlation coeffi cients among the full-sib family estimated breeding values (EBVs) obtained from the three models were greater than 0.998 6 and all models produced an almost identical family ranking. The accuracies of selection obtained with the CSL, THRp, and THRl model were 0.773 8, 0.775 4, and 0.784 4, respectively, the greatest from the THRl model. The genetic correlations between body weight and survival data EBVs from the CSL, THRp, and THRl models were 0.020 1,-6.201 1×10^-4 , and -3.115 4×10^-4 , respectively, and the phenotypic correlations between the two traits were -0.837 1 and -0.667 1, respectively. The findings of this study provide background information to determine the best strategy of selection for the genetic improvement of UTT in turbot.

关键词： Scophthalmus maximus upper thermal tolerance genetic parameters cross-sectional models

Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2020年第2期38卷 467-479页

作者： CUI Wenxiao ma aijun HUANG Zhihui WANG Xin’an SUN Zhibin LIU Zhifeng ZHANG Wei YANG Jingkun ZHANG Jinsheng QU Jiangbo College of Fisheries and Life Science Shanghai Ocean UniversityMinistry of EducationShanghai 201306China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShandong Key Laboratory of Marine Fisheries Biotechnology and Genetic BreedingQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyQingdao 266071China Yantai Tianyuan Aquatic Limited Corporation Yantai 264003China

Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory *** kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5)and seawater(salinity 30)was sequenced and *** silico analysis indicated that all unigenes had significant hits in seven *** functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion,absorption,and metabolism played an important role in the osmoregulation of turbot in response to *** of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic ***,we analyzed and predicted the mechanisms of canonical signaling *** processes involved in renin secretion,ECM-receptor interaction,adherens junction,and focal adhesion played an important role in the plasticity phenotype in hypo-stress,while the signal transduction network composed of the maPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse,worked in hypoosmoregulation signal transduction in the *** addition,analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried *** results showed that the kidney,gill,and spleen were vital regulating organs of osmotic pressure,and the osmoregulation pattern of euryhaline fish dif fered among species.

关键词： osmoregulation mechanisms Scophthalmus maximus hypo-saline signal transduction cell volume regulation gene expression

Molecular characterization and expression analysis of Lily- type lectin (SmLTL) in turbot Scophthalmus maximus, and its response to Vibrio anguillarum

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2018年第2期36卷 508-518页

作者： XIA Dandan ma aijun HUANG Zhihui SHANG Xiaomei CUI Wenxiao YANG Zhi QU Jiangbo Key Laboratory of Sustainable Development of marine Fisheries Ministry of Agriculture Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Ocean University of Shanghai Shanghai 201306 China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Yantai Tianyuan Aquatic Limited Corporation Yantai 264006 China

A full-length lily-type lectin （SmLTL） was identified from turbot （Scophthalmus maximus） in this study. By searching database for protein identification and fimction prediction, SmLTL were confirmed. The full-length cDNA of SmLTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The SmLTL peptide is characterized by a specific J3-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30-99; two of the repeats share the classical mannose binding domain （QxDxNxVxY） while the third binding site was similar to other fish-specific binding motifs （TxTxGxRxV）. The primary, secondary, and tertiary structures of SmLTL were predicted and analyzed, indicating that the SmLTL protein was hydrophilic, contained 5.36% a-helices, 39.29% extended strands, 16.07% [3-folds, and 39.29% random coils, and three [3-folds. Quantitative real- time polymerase chain reaction （qPCR） analysis revealed that the SmLTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of SmLTL expression were observed in other tissues. The expression of SmLTL in gill, skin and intestine increased at mRNA level after stimulation of Hbrio anguillarum, our results suggest that SmLTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that SmLTL is a member of the lily- type lectin family and the information reported here will provide an important foundation for future research on the role of this protein.

关键词： lily-type lectin full-length of cDNA quantitative real-time polymerase chain reaction protein structure Vibrio anguillarum

利用内部骨重建理论和有限元法计算骨密度的变化

在线全文

学校读者我要写书评

暂无评论

生物医学工程学杂志 2006年第1期23卷 60-63页

作者：安梅岩马爱军航天医学工程研究所北京100094

采用内部骨重建理论和有限元算法相结合的方法,探讨了外载荷对骨重建的作用。从一张骨的X线断层片提取出边界数据,处理后建立骨的二维模型;应用有限元软件A nsys进行计算,得到骨重建后的结果。计算结果表明内部重建理论预测结果与骨的... 详细信息

采用内部骨重建理论和有限元算法相结合的方法,探讨了外载荷对骨重建的作用。从一张骨的X线断层片提取出边界数据,处理后建立骨的二维模型;应用有限元软件A nsys进行计算,得到骨重建后的结果。计算结果表明内部重建理论预测结果与骨的实际结构有相当程度的一致性,证明了此种方法的可行性。

关键词：骨重建内部骨重建模型有限元

基于C-R模型比较大菱鲆快速生长品系和普通养殖群体的生长特征

在线全文

学校读者我要写书评

暂无评论

水产学报 2018年第6期42卷 932-940页

作者：王新安马爱军中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室青岛市海水鱼类种子工程与生物技术重点实验室青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室

大规模家系选育技术也称为现代育种技术,具有选择效率高、可抑制遗传衰退等优点,是进行分子辅助育种技术的基础。基于此,采用这项技术开展了大菱鲆快速生长性状遗传改良研究。为了系统评估大菱鲆快速生长选育的育种成效,运用C-R模型分... 详细信息

大规模家系选育技术也称为现代育种技术,具有选择效率高、可抑制遗传衰退等优点,是进行分子辅助育种技术的基础。基于此,采用这项技术开展了大菱鲆快速生长性状遗传改良研究。为了系统评估大菱鲆快速生长选育的育种成效,运用C-R模型分别对选育出的大菱鲆快速生长品系和普通养殖群体的非线性生长曲线进行了拟合和比较分析,深入揭示选育出的大菱鲆快速生长品系和普通养殖群体的生长差异。研究结果显示,快速生长品系和普通养殖群体C-R模型的拟合度、拐点月龄、拐点体质量、最大月增体质量分别为0.999和0.997、15.228和16.858月龄、716.712和619.862 g、111.711和78.061 g;快速生长品系的瞬时生长率、瞬时生长加速率、相对生长率分别在4.5~25月龄、3~13.8月龄、3~15月龄,高于普通养殖群体,在其他相应阶段,快速生长品系的瞬时生长率和瞬时生长加速率低于普通养殖群体,而相对生长率无显著差异;快速生长品系的始速点、拐点和终速点均比普通养殖群体提前,并且快速生长期时间区间长度(12.35月龄)小于普通养殖群体(15.28月龄),其对应的体质量区间长度(1 187.680 g)大于普通养殖群体(1 027.120 g),即基于家系选育技术获得的大菱鲆快速生长品系在较短的快速生长时间区间范围内,获得了更大的净增体质量,取得了更大的遗传进展。

关键词：大菱鲆 C-R模型快速生长品系生长特征