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Role of <i>Helicobacter pylori</i>Infection in Recurrent Abdominal Pain of the Child in brazzaville

Open Journal of Pediatrics 2020年第4期10卷 587-599页

作者： Ollandzobo Ikobo Lucie Charlotte ahombo niotsebe lydie bénédicte Mongo-Onkouo Arnaud Ahoui Apendi Clausina Rochelvie Itoua ngaporo ngala Akoa Mimiesse Mounanou Jile Florent Tchidjo ngamo Lynda Déby Gassaye Mabiala babela Jean Robert Atipo Ibara blaise Irénée Ibara Jean-Rosaire Faculty of Health Sciences Marien Ngouabi University of Brazzaville Brazzaville Congo Infants Pediatric ward Brazzaville University Teaching Hospital Brazzaville Congo Gastroentérology and Internal Medicine Ward Brazzaville University Teaching Hospital Brazzaville Congo

The Helicobacter pylori (Hp) infection is a public health problem, especially in developing countries. However, the responsibility of Hp for the occurrence of recurrent abdominal pain is still unclear. Objectives: To ... 详细信息

The n>Helicobacter pylori (Hp) infection is a public health problem, especially in developing countries. However, the responsibility of Hp for the occurrence of recurrent abdominal pain is still unclear. b>Objectivesb>: To determine the prevalence of Helicobacter pylori (Hp) infection in children with recurrent abdominal pain (RAP) in Brazzaville and to establish the relationsn>hip between Hp infection and RAP. b>Patientsb> b>andb> b>Methods:b> We conducted a cross-sectional case-control study, between March and September 2018, including 106 children with RAP and 218 children without RAP, aged 2 to 17 years old, received in health centres in Brazzaville. All the children were subjected to detection of Hp fecal antigen by qualitative immuno-chromatography in the faeces. b>Results:b>n style="font-family:Verdana;"> The prevalence of Hp infection among children with RAP in brazzaville was 43.4%. The female sex was most represented in both populations, with a sex ratio of 0.84 in the infected population. Compared with controls, these were children with a mean age of 8.13 ± 4.57 years. Infected children with RAP were unschooled in 66.7% (n = 31) of cases (p ng potty were 63% (n = 29). Handwashing before meals and after using the toilet was not done in 66.7% (n = 31) and 56.5%

关键词： Helicobacter pylori Recurrent Abdominal Pain Fecal Antigen Child brazzaville

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Antibiotic Resistance Phenotypes of <i>Enterobacteriaceae</i>Isolated from Household Wastewater in brazzaville, Republic of Congo

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Advances in Microbiology 2021年第1期11卷 27-36页

作者： Rachel Moyen Tarcisse baloki ngoulou Etienne nguimbi Gabriel ahombo Laboratory of Cellular and Molecular biology Sciences and Techniques Faculty University Marien NGOUABI Brazzaville Congo

Household wastewater is a source of pollution and can present health risks when discharged into the environment. Thus, samples of household wastewater from a few neighborhoods in brazzaville were analyzed for microbiological quality. The various samples were cultured for isolation on solid media using conventional microbiological methods. The bacteria isolated were identified by the Enterobacter System gallery. Sensitivity tests were performed using the standard antibiotic susceptibility test by diffusion on Mueller Hinton medium. At the end of the analysis, 51 Enterobacteriaceae were isolated and identified. They included: 8 (15.68%) Escherichia coli, 8 (15.68%) Salmonella spp., 8 (15.68%) Shigella spp., 8 (15.68%) Klebsiella spp., 5 (9.80%) Enterobacter aerogenes, 8 (15.68%) Enterobacter cloacae, 3 (5.90%) Arizona spp., 3 (5.90%) Proteus spp. The results obtained show that the bacteria tested showed total resistance to the following antibiotics: amoxicillin, amoxicillin + clavulanic acid, cloxacillin and nalidixic acid. On the other hand, imipenem, cefuroxime, cefotaxime, cefftriazone and kanamycin were the most active antibiotics with low levels of resistance. The low resistance rates observed for imipenem, cefotaxime, cefuroxime and cefftriazone show that these antibiotics can be used for the treatment of infections caused by household wastewater bacteria.

关键词： Antibiotic Therapy Enterobacteriaceae Sewage Households

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Cardiovascular Disorders in Cirrhosis at the brazzaville Teaching Hospital in 2022

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Open Journal of Gastroenterology 2023年第11期13卷 369-376页

作者： Rody Stéphane ngami Philestine Clausina Mikolele Ahoui Apendi Relda Itoko Okombi Christian Kouala Landa Marlyse ngalessami Mouakosso bénédicte ahombo niotsombe ngala Akoa Itoua-ngaporo Pérès Mardochée Motoula Latou Jile Florient Mimiesse Monamou Arnaud Mongo-Onkouo Deby Gassaye blaise Irénée Atipo Ibara Department of Gastroenterology and Internal Medicine University Teaching Hospital Brazzaville Republic of the Congo Faculty of Health Sciences Marien Ngouabi University Brazzaville Republic of the Congo Department of Cardiology and Internal Medicine University Teaching Hospital Brazzaville Republic of the Congo

Cirrhosis is a liver disease that can lead to cardiovascular lesions that are often asymptomatic but potentially fatal. Objective: The aim of this study was to evaluate electrical and echographic cardiovascular abnormalities during cirrhosis at the brazzaville University Hospital. Patients and Methods: We conducted a cross-sectional analytical study over a 2-year period at the brazzaville Universitary Hospital. Cirrhotic patients at least 18 years of age who had undergone electrocardiogram and echocardiography were included. Patients with cardiac cirrhosis, hepatocellular carcinoma or spontaneous bacterial peritonitis were excluded. The outcome variables were cardiac electrical and echographic abnormalities. Data were analyzed using Epi info 7.2 software. Pearson’s chi-square, Fisher’s exact and Student’s exact tests were used to compare proportions and means at a significance level of 0.05. Results: A total of 76 cirrhotic patients were recruited out of 186 patients, i.e. 40.9% of cirrhotic patients. There were 48 men and 28 women, for a sex ratio of 1.7. The median age was 56 (IQR 41.5 - 69.0). QTc prolongation, microvoltage and sinus tachycardia were the most frequent electrical disorders, respectively in 19 (29.7%), 11 (17.2%) and 11 (17.2%) cases. Electrical disorders were statistically related to cirrhosis stage (p = 0.0364). The most frequent ultrasound disorders were left ventricular systolic failure 11 (28.9%) and dilated cardiomyopathy 8 (21.1%). Seven (9.2%) patients had high pulmonary arterial pressure. Death, observed in 12 cases (15.8%), was statistically linked to the presence of echocardiographic disorders (p = 0.0089) and congestive heart failure (p = 0.0001). Conclusion: The search for cardiac disorders should be systematic during decompensated cirrhosis to detect potentially fatal abnormalities.

关键词： Cirrhosis ECG Echocardiography Cardiovascular Disorders

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Tumor necrosis Factor-Alpha (TnF)-308G/A and Interleukin 8(IL-8)-251C/T Polymorphisms in Pulmonary Tuberculosis Patients from Congo

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Open Journal of Immunology 2023年第1期13卷 1-13页

作者： Faust René Okamba Prudence Spinelie Koumba Pambou Mandingha Kosso Etoka-beka brave nzoussi Regis Gothard bopaka Cyr Jonas Morabandza Gabriel ahombo Faculty of Health Sciences Marien Ngouabi University Brazzaville Republic of Congo Laboratory of Immunology National Institute of Health Science and Research (IRSSA) Brazzaville Republic of Congo Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of Congo

background: Tuberculosis (Tb) is one of the world’s deadliest infectious diseases. Tumor necrosis factor-Alpha (TnF-α) and Interleukin 8 (IL-8) are involved in the pathogenesis of pulmonary Tb (PTb). However, the contribution of polymorphisms of these cytokines to PTb susceptibility needed more investigation across geographic regions and ethnic groups. Purpose: The aim of this study was to investigate the association of the TnF-α-308 G/A and IL-8-251T/A polymorphisms with PTb risk in the Congolese population. Methods: This case-control study included 150 PTb patients and 160 control subjects. blood samples were collected from all participants and were used for the TnF-α-308 G/A and IL-8-251T/A genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Odds ratios (OR) were calculated to estimate the potential polymorphism associations. A P level of Results: A significant difference was found between PTb patients and controls regarding the TnF-α-308AA genotype (P = 0.035) distribution. Moreover, this genotype was associated with risk to Tb (OR = 7.19, 95% CI = 0.85 - 60.65, P = 0.035). The A allele was significantly more frequent in PTb patients than in controls, and was associated with risk to PTb (OR = 1.68, 95% CI = 1.05 - 2.68, P = 0.014). Regarding the IL-8-251T/A gene, TA and AA genotypes were significantly more frequent in PTb patients compared to controls, and were associated with increased risk to PTb (OR = 2.64, 95% CI = 0.97 - 7.18, P = 0.031 and OR = 3.0, 95% CI = 1.13 - 7.98, P = 0.014, respectively). However, the IL-8-251 A allele was not associated to PTb susceptibility (OR = 0.27, 95% CI = 0.15 - 0.44). Conclusion: TnF-α-308G/A and IL-8-251T/A polymorphisms may be associated to PTb susceptibility in the Congolese population, and the AA genotype of both cytokines could be a risk factor.

关键词： Pulmonary Tuberculosis Cytokine Polymorphism Tumor necrosis Factor-Alpha Interleukin-8 PCR-RFLP

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Antibiotic Resistance Profile of Pathogenic bacteria Isolated from “Mabokés” Smothered Fish in brazzaville, Congo

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Journal of biosciences and Medicines 2020年第8期8卷 138-148页

作者： Morabandza Cyr Jonas nguimbi Etienne baloki ngoulou Tarcisse Moyen Rachel Moussounda Milongo Lucien Jolivet Soloka Mabika Armel Faly ngo-Itsouhou Christian ahombo Gabriel normal Superior School Marien NGOUABI University Brazzaville Republic of Congo Cellular and Molecular biology Laboratory Faculty of Sciences and Techniques Marien NGOUABI University Brazzaville Republic of Congo

The smothered fish samples were taken from 3 markets. They were grown on different selective and differentiated culture media to target groups of bacteria associated with food poisoning. Isolates were identified on the basis of cellular and colonial morphologies on selective and differentiated culture media, followed by susceptibility testing to certain families of antibiotics, in particular beta-lactams. This study showed that S. aureus and b. cereus had high levels of beta-lactam resistance. However, these strains were sensitive to kanamycin, tobramycin, ciprofloxacin and norfloxacin. The characteristic penicillinase phenotype was dominant in Gram-positive bacteria. Shigella spp, Salmonella spp and E. coli were resistant to beta-lactam antibiotics. Tobramycin and meropenem retained their activity on all strains. Despite the increased rates of resistance observed, vancomycin, kanamycin, tobramycin, gentamicin, ciprofloxacin, and norfloxacin can be used in the treatment of community-acquired infections caused by Gram-positive bacteria while meropenem and tobramycin for Shigella spp, Salmonella spp and E. coli infections.

关键词： Resistance Antibiotics bacteria Smothered Fish

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DR Congo develops with bRI

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一带一路报道(中英文) 2023年第1期 19-19页

作者： Liliane ahombo the Center for Strategic Studies and International Security in DR Congo

The year 2022 is especially significant for two reasons: it marks the 50th anniversary of the normalization of diplomatic relations between China and the Democratic Republic of the Congo and witnessed the convening of...

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Giving More benefits to biosurfactants Secreted by Lactic Acid bacteria Isolated from Plantain Wine by Using Multiplex PCR Identification

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Advances in Microbiology 2019年第11期9卷 917-930页

作者： Michaelle bokamba Moukala Christian Aimé Kayath Gabriel ahombo nicole Prisca Makaya Dangui Duchel Jeanedvi Kinouani Kinavouidi Effort Concentré nguimbi Mouélé Martin Diatewa Laboratoire de biologie Cellulaire et Moléculaire (bCM) Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville Congo Institut national de Recherche en Sciences Exactes et naturelles (IRSEn) Brazzaville Congo Ecole normale Supérieure Université Marien Ngouabi Brazzaville Congo

Fermented beverages have continued to give more surprises in terms of the presence of biomolecules and the diversity of microorganisms that may be contained. Republic of Congo is home to a panoply of fermented foods and beverages that are still not yet studied. This is the case of plantain wine fluently called banana wine. Within this framework, this work aims to study the role of biosurfactant-like biomolecules secreted during fermentation of plantain wine. Using MRS medium, 15 isolates bacteria have been found. 100% are able to secrete biosurfactant and 66.66% are extractible biosurfactants. 33% of isolates have been associated to Lactobacillus plantarum (Is2, Is9, Is12 and Is13) by using a one-step multi-plex PCR that targets genes encoding for bacteriocins. biosurfactants secreted by L. plantarum play an important role in the preservation of banana wine. The biosurfactants extracted with chloroform and ammonium sulphate are able to inhibit the growth of pathogenic bacteria including Shigella flexneri, Salmonella spp., Pseudomonas aeruginosa and Staphy-lococcus aureus.

关键词： Plantain Wine Fermented beverage Lactobacillus spp. Multiplexe PCR biosurfactants Pathogenic Inhibition

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Prevalence of Hepatitis C Virus Infection and Risk Factors among Pregnant Women in Pointe noire, Republic of Congo

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Journal of biosciences and Medicines 2019年第8期7卷 84-93页

作者： brunel M. Angounda Serge O. Mokono ngala Itoua-ngaporo Jourdain b. nziengue M’vouala Jile F. Mimiesse Clausina M. Ahoui Apendi Gabriel ahombo blaise I. Atipo Ibara Jean Rosaire Ibara Faculty of Science and Technology Marien NGOUABI University Brazzaville Congo national Center of blood Transfusion Brazzaville Congo Faculty of Health Sciences Marien NGOUABI University Brazzaville Congo Hospital and University Center of brazzaville Brazzaville Congo

background: Hepatitis C virus (HCV) is a public health problem and is highly endemic in Sub-Saharan Africa. This study was carried out to determine the prevalence and potential risk factors of hepatitis C virus in pregnant women. Methods: This was a cross-sectional study involving pregnant women who attended at seven antenatal clinics in Pointe noire, from June to november 2018. Structured questionnaires were used to obtain participants data. Samples were screened for HCV infection by using 4th ELISA methods and data analysis was done by Epi-info version 7.0 statistical software. Results: A total of 150 pregnant women were included, out of which 11 (7.3%) were positive for HCV. The high proportion of HCV was found in age group 25 - 34 years (45.4%), educated (81.1%), professional (36.4%) and 54.6% were in second trimester gestational stage. Moreover, 54.6% of HCV positive patients had scarification and 45.5% had a history of surgery. There was not a statistically significant association between demographics characteristics, risks factors and HCV seropositivity (p > 0.05). Conclusion: This study showed a high prevalence of HCV infection and confirmed that this infection is a public health problem in pregnant women in Pointe noire.

关键词： Pregnancy HCV Risk Factors

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