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Molecular characterization of an outbreak-involved Bacillus anthracis strain confirms the spillover of anthrax from West Africa

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Infectious Diseases of Poverty 2024年第1期13卷 93-94页

作者： Shuchao Wang Roland Suluku Mohamed B.Jalloh ahmed f.samba Baogui Jiang Yubiao Xie Doris Harding Mengyao Zhang foday Sahr Mahmud E.Sesay James S.Squire Mohamed A.Vandi Moinina N.Kallon Shoufeng Zhang Rongliang Hu Yuee Zhao Zhiqiang Mi Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China Department of Animal Sciences School of Agriculture and Food Sciences Njala University Njala Sierra Leone Department of Microbiology College of Medicine and Allied Health Sciences University of Sierra Leone Freetown Sierra Leone Ministry of Agriculture and food Sciences Freetown Sierra Leone Beijing Institute of Microbiology and Epidemiology Beijing China Ministry of Health and Sanitation Freetown Sierra Leone Beijing Institute of Biotechnology Beijing China

Background Anthrax,a zoonotic disease caused by the spore-forming bacterium Bacillus anthracis,remains a major global public health concern,especially in countries with limited *** Leone,a West African country historically plagued by anthrax,has almost been out of report on this disease in recent *** this study,we described a large-scale anthrax outbreak affecting both animals and humans and attempted to characterize the *** The causative agent of the animal outbreak in Port Loko District,Sierra Leone,between March and May 2022 was identified using the nanopore sequencing technique.A nationwide active surveillance was implemented from May 2022 to June 2023 to monitor the occurrence of anthrax-specific symptoms in *** cases were subsequently verified using quantitative polymerase chain ***-genome sequencing was accomplished by combining long-read and short-read sequencing *** phylogenetic analysis was performed based on the full-chromosome single nucleotide *** The outbreak in Port Loko District,Sierra Leone,led to the death of 233 animals between March 26th and May 16th,*** ruled out the initial suspicion of Anaplasma species and successfully identified *** as the causative agent of the *** a result of the government's prompt response,out of the 49 suspected human cases identifed during the one-year active surveillance,only 6 human cases tested positive,all within the frst month after the official declaration of the *** phylogenetic analysis indicated that the BaSL2022 isolate responsible for the outbreak was positioned in the ***.153 clade within the TransEuroAsian group of *** We successfully identifed a large-scale anthrax outbreak in Sierra *** causative isolate of ***,BaSL2022,phylogenetically bridged other lineages in ***.153 clade and neighboring genetic groups,***.144 and ***.148,

关键词： Molecular identification Anthrax Bacillus anthracis Sierra Leone Phylogeny Single nucleotide polymorphism

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Classical taxonomy,molecular phylogeny and genetic analysis of the genus Exitianus Ball,1929(Hemiptera:Cicadellidae:Deltocephalinae)from Egypt

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Zoological Systematics 2024年第2期49卷 143-157页

作者： Rabab M.El-Hady ahmed f.El-Sayed Survey&Taxonomy Department Plant Protection Research InstituteAgriculture Research CentreGizaEgypt Microbial Genetics Department Biotechnology Research InstituteNational Research CentreGizaEgypt Egypt Center for Research and Regenerative Medicine(ECRRM) CairoEgypt

Egyptian species of the leafhopper genus Exitianus Ball,1929,***(St?l,1855),***(Distant,1908),and *** Ross,1968 are ***,morphological descriptions,and a key for their identification are *** this study,we used molecular techniques to confirm morphological identification and detect phylogeny among the three Exitianus *** partial nucleotide sequences of the amplified products obtained were determined by Macrogen *** nucleotide sequences of 28S rDNA and COX genes of the EGY-ARC-9,EGY-ARC-4,and EGY-ARC-5 were determined by Macrogen Korea,blasted into BLAST at the National Center for Biotechnology Information website(NCBI)and compared with those deposited in the GenBank DNA *** results represented the homology percentage between the partial sequences of the 28S rRNA and COX genes from each species and related species obtained from GenBank DNA *** morphological identifications of the three Exitianus species were confirmed by molecular characterization and sequencing of 28S rDNA and COX genes and identified as ***,***,and ***,their sequences of 28srDNA and COX genes were deposited in GenBank with an accession number(LC670610,LC670607,and OQ196105)for 28srDNA gene and(LC775357,LC775358,and LC775359)for the COX gene.

关键词： Auchenorrhyncha Chaismini taxonomic 28S rDNA phylogenetic

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A Comprehensive Review on Charging Topologies and Power Electronic Converter Solutions for Electric Vehicles

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 675-694页

作者： Abdelfatah Ali Hossam H.H.Mousa Mostafa f.Shaaban Maher A.Azzouz ahmed S.A.Awad the Department of Electrical Engineering American University of Sharjah26666 SharjahUnited Arab Emirates the Department of Electrical Engineering South Valley University83523 QenaEgypt the Department of Electrical and Computer Engineering University of WindsorWindsorON N9B 3P4 Canada Electrical Engineering Department Qatar UniversityDoha 2713Qatar Department of Electrical and Computer Engineering Sultan Qaboos UniversityMuscat123Oman

Electric vehicles(EVs)are becoming more popular worldwide due to environmental concerns,fuel security,and price *** performance of EVs relies on the energy stored in their batteries,which can be charged using either AC(slow)or DC(fast)***,EVs can also be used as mobile power storage devices using vehicle-to-grid(V2G)*** electronic converters(PECs)have a constructive role in EV applications,both in charging EVs and in ***,this paper comprehensively investigates the state of the art of EV charging topologies and PEC solutions for EV *** examines PECs from the point of view of their classifications,configurations,control approaches,and future research prospects and their impacts on power *** can be classified into various topologies:DC-DC converters,AC-DC converters,DC-AC converters,and AC-AC *** address the limitations of traditional DC-DC converters such as switching losses,size,and high-electromagnetic interference(EMI),resonant converters and multiport converters are being used in high-voltage EV ***,power-train converters have been modified for high-efficiency and reliability in EV *** paper offers an overview of charging topologies,PECs,challenges with solutions,and future trends in the field of the EV charging station applications.

关键词： Charging station charging topology electric vehicle power electronic converter vehicle-to-grid

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Study of scalar particles through the Klein–Gordon equation under rainbow gravity effects in Bonnor–Melvin-Lambda space-time

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Communications in Theoretical Physics 2024年第4期76卷 113-125页

作者： faizuddin ahmed Abdelmalek Bouzenada Department of Physics University of Science&Technology MeghalayaRi-Bhoi793101India Laboratory of theoretical and applied Physics Echahid Cheikh Larbi Tebessi UniversityAlgeria

In our investigation,we explore the quantum dynamics of charge-free scalar particles through the Klein–Gordon equation within the framework of rainbow gravity,considering the Bonnor–Melvin-Lambda(BML)space-time *** BML solution is characterized by the magnetic field strength along the axis of the symmetry direction which is related to the cosmological constantΛand the topological parameterαof the *** behavior of charge-free scalar particles described by the Klein–Gordon equation is investigated,utilizing two sets of rainbow functions:(i)f(χ)=■,h(χ)=1 and(ii)f(χ)=1,h(χ)=1+βХ/*** 0for the scalar particles and conduct a thorough analysis of the obtained ***,we study the quantum dynamics of quantum oscillator fields within this BML space-time,employing the Klein–Gordon *** also,we choose the same sets of rainbow functions and obtain the approximate eigenvalue solution for the oscillator ***,we demonstrate that the relativistic approximate energy profiles of charge-free scalar particles and oscillator fields get influenced by the topology of the geometry and the cosmological ***,we show that the energy profiles of scalar particles receive modifications from the rainbow parameter and the quantum oscillator fields by both the rainbow parameter and the frequency of oscillation.

关键词： quantum fields in curved space-time relativistic wave equations solutions of wave equations bound-state special functions

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Diagnostic accuracy of ePOS score in predicting DNR labeling after ICU admission:A prospective observational study(ePOS-DNR)

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Journal of Intensive Medicine 2024年第2期4卷 216-221页

作者： Omar E.Ramadan ahmed f.Mady Mohammed A.Al-Odat ahmed N.Balshi ahmed W.Aletreby Taisy J.Stephen Sheena R.Diolaso Jennifer Q.Gano Waleed Th.Aletreby Department of Critical Care King Saud Medical CityRiyadhSaudi Arabia Anesthesia Department Faculty of MedicineAin Shams UniversityCairoEgypt Anesthesia Department Faculty of MedicineTanta UniversityTantaEgypt faculty of Medicine Alexandria UniversityAlexandriaEgypt Nursing Department King Saud Medical CityRiyadhSaudi Arabia

Background Resuscitation can sometimes be futile and making a do-not-resuscitate(DNR)decision is in the best interest of the *** electronic poor outcome screening(ePOS)score was developed to predict 6-month poor outcomes of critically ill *** explored the diagnostic accuracy of the ePOS score in predicting DNR decisions in the intensive care unit(ICU).Methods This study was conducted at the ICU of a tertiary referral hospital in Saudi Arabia between March and May ***,we calculated ePOS scores for all eligible consecutive admissions after 48 h in the ICU and recorded the DNR *** ability of the score to predict DNR was explored using logistic ***'s ideal cut-off value was calculated using the DeLong method,and different diagnostic accuracy measures were generated with corresponding 95%confidence intervals(CIs).Results We enrolled 857 patients,125 received a DNR order and 732 did *** average ePOS score of DNR and non-DNR patients was 28.2±10.7 and 15.2±9.7,*** score,as a predictor of DNR order,had an area under receiver operator characteristic(AUROC)curve of 81.8%(95%CI:79.0 to 84.3,Pff value>17 was associated with a sensitivity of 87.2(95%CI:80.0 to 92.5,Pficity of 63.9(95%CI:60.3 to 67.4,Pf 29.2(95%CI:24.6 to 33.8,Pf 96.7(95%CI:95.1 to 98.3,Pformed well as a diagnostic test for patients who will be labeled as DNR during their ICU stay.A cut-off score>17 may help guide clinical decisions to withhold or commence resuscitative measures.

关键词： Do-not-resuscitate(DNR) Resuscitation Intensive care unit(ICU) Diagnostic accuracy Sensitivity Specificity

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A Hybrid Classification and Identification of Pneumonia Using African Buffalo Optimization and CNN from Chest X-Ray Images

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Computer Modeling in Engineering & Sciences 2024年第3期138卷 2497-2517页

作者： Nasser Alalwan ahmed I.Taloba Amr Abozeid ahmed Ibrahim Alzahrani Ali H.Al-Bayatti Computer Science Department Community CollegeKing Saud UniversityRiyadh11437Saudi Arabia Information System Department Faculty of Computers and InformationAssiut UniversityAssiutEgypt Mathematics Department Faculty of ScienceAl-Azhar UniversityCairoEgypt Cyber Technology Institute(CTI) De Montfort UniversityLeicesterUK

An illness known as pneumonia causes inflammation in the *** there is so much information available fromvarious X-ray images,diagnosing pneumonia has typically proven *** improve image quality and speed up the diagnosis of pneumonia,numerous approaches have been *** date,several methods have been employed to identify *** Convolutional Neural Network(CNN)has achieved outstanding success in identifying and diagnosing diseases in the fields of medicine and ***,these methods are complex,inefficient,and imprecise to analyze a big number of *** this paper,a new hybrid method for the automatic classification and identification of Pneumonia from chest X-ray images is *** proposed method(ABOCNN)utilized theAfrican BuffaloOptimization(ABO)algorithmto enhanceCNNperformance and *** Weinmed filter is employed for pre-processing to eliminate unwanted noises from chest X-ray images,followed by feature extraction using the Grey Level Co-Occurrence Matrix(GLCM)*** features are then selected from the dataset using the ABO algorithm,and ultimately,high-performance deep learning using the CNN approach is introduced for the classification and identification of *** results on various datasets showed that,when contrasted to other approaches,the ABO-CNN outperforms them all for the classification *** proposed method exhibits superior values like 96.95%,88%,86%,and 86%for accuracy,precision,recall,and f1-score,respectively.

关键词： African buffalo optimization convolutional neural network pneumonia X-ray

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A review:the mechanism of plant-derived polysaccharides on osteoblasts and osteoclasts

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未来食品学报(英文) 2024年第3期4卷 183-192页

作者： Mengjie Ren Adel f.ahmed Meng Li Menghan Li Zhiruo Yan Jinmei Wang National R&D Center for Edible fungus Processing Technology Henan UniversityKaifeng 475004China Joint International Research Laboratory of food&Medicine Resource function Henan ProvinceKaifeng 475004China Medicinal and Aromatic Plants Researches Department Horticulture Research InstituteAgricultural Research CenterGiza 12511Egypt

Bone loss and deterioration of bone microarchitecture would increase the bone fragility and fracture risk,leading to the *** and more evidences proved that plant-derived polysaccharides could have a remarkable influence on osteoblasts and osteoclasts,exerting anti-osteoporosis *** to the previous research,the extract of Cibotium barumoz,Achyranthes bidentata,Curculigo orchioides,Epimedium brevicornum,Angelica sinensis,Polygonatum sibiricum,Dendrobium officinale,Morinda officinalis,Nelumbo mucifera,Diospyros kaki,Hordeum vulgare,Cistanche deserticola,Commiphora Myrrha and other plant-derived polysaccharides could benefit to the osteoblasts and *** essential mechanisms are mainly related to the activation or inhibition of many factors,including runt-related transcription factor 2(Runx2),B-catenin,osterix(Osx),activator protein-1(AP-1),osteocalcin(OCN/BGP),alkaline phosphatase(ALP),osteopontin(OPN),bone morphogenetic protein(BMP),phosphatidylinositol 3-kinase(PI3K)/C-Jun N-terminal kinase(JNK)/extracellular regulated protein kinase(ERK),osteoprotegerin(OPG),receptor activator of nuclear factor-kB(RANK),monocyte/macrophage colony-stimulating factor(M-CSf),tumor necrosis factor receptor-associated factor 6(TRAf-6),receptor activator of nuclear factor(Nf)-KB ligand(RANKL),nuclear factor of activated T cells 1(NfATc1),c-fos,matrix metallopeptidase-9(MMP-9),glycogen synthase kinase 3β(GSK3B)/B-catenin,nuclear factor E2-related factor 2(Nf2),as well as these related pathways,such as Wnt/p-catenin,BMP-2/SMAD1/5/8,PI3K/AKT,OPG/RANKL/RANK,Nf-κB,MAPKs,*** plant-derived polysaccharides could improve the dynamic balance of bone formation and resorption through promoting the differentiation and maturation of osteoblast or inhibiting its *** reviewed plant-derived polysaccharides and their regulating mechanisms on the osteoclasts and osteoblasts provide the evidences for the development of osteoporosis therapeutics.

关键词： Osteoporosis Plant-derived polysaccharides Osteogenesis promotion Osteoclasts inhibition

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Analysis of b→cτν¯_(τ)anomalies using weak effective Hamiltonian with complex couplings and their impact on various physical observables

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Chinese Physics C 2024年第8期48卷 56-75页

作者： Muhammad Arslan Tahira Yasmeen Saba Shafaq Ishtiaq ahmed Muhammad Jamil Aslam Department of Physics Quaid-i-Azam UniversityIslamabad 45320Pakistan Department of Physics International Islamic UniversityIslamabad 44000Pakistan National Center for Physics Islamabad 44000Pakistan

Recently,the experimental measurements of the branching ratios and different polarization asymmetries for processes occurring through flavor-changing-charged current(b→cτν¯_(τ))transitions by BABAR,Belle,and LHCb have revealed some significant differences from the corresponding Standard Model(SM)*** has triggered an interest to search for physics beyond the SM in the context of various new physics(NP)models and using the model-independent weak effective Hamiltonian(WEH).Assuming left-handed neutrinos,we add the dimension-six vector,(pseudo-)scalar,and tensor operators with complex Wilson coefficients(WCs)to the SM *** 60%,30%,and 10%constraints resulting from the branching ratio of B_(c)→τν¯_(τ),we reassess the parametric space of these new physics WCs accommodating the current anomalies based on the most recent HfLAV data of R_(τ/μ,e)(D)and R_(τ/μ,e)(D^(*))and Belle data of f_(L)(D^(*))and P_(τ)(D^(*)).We find that the allowed parametric region of left-handed scalar couplings strongly depends on the constraints of the B_(c)→τν¯_(τ)branching ratio,and the maximum pull from the SM predictions results from theficantly if we extend the analysis by adding LHCb data of R_(τ/μ)(J/ψ)and R_(τ/ℓ)(Λ_(c)).furthermore,due to the large uncertainties in the measurements of R_(τ/μ)(J/ψ)and R_(τ/ℓ)(X_(c)),we derive the sum rules which complement them with R_(τ/μ,e)(D)and R_(τ/μ,e)(D^(*)).Using the best-fit points of the new complex WCs along with the latest measurements of R_(τ/μ,e)(D(^(*))),we predict the numerical values of the observable R_(τ/ℓ)(Λ_(c)),R_(τ/μ)(J/ψ),and R_(τ/ℓ)(X_(c))from the sum *** simultaneous dependence of abovementioned physical observables on the NP WCs is established by plotting their correlation with R_(D) and R_(D^(*)),which are useful to discriminate between various NP *** find that the most significant impact of NP results from the WC C^(S)_(L)=4C^(T).fi

关键词： B meson anomalies new physics complex coupling HfLAV

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Enhanced Object Detection and Classification via Multi-Method fusion

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Computers, Materials & Continua 2024年第5期79卷 3315-3331页

作者： Muhammad Waqas ahmed Nouf Abdullah Almujally Abdulwahab Alazeb Asaad Algarni Jeongmin Park Department of Computer Science Air UniversityIslamabad44000Pakistan Department of Information Systems College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia Department of Computer Science College of Computer Science and Information SystemNajran UniversityNajran55461Saudi Arabia Department of Computer Sciences Faculty of Computing and Information TechnologyNorthern Border UniversityRafha91911Saudi Arabia Department of Computer Engineering Tech University of Korea237 Sangidaehak-roSiheung-siGyeonggi-do15073South Korea

Advances in machine vision systems have revolutionized applications such as autonomous driving,robotic navigation,and augmented *** substantial progress,challenges persist,including dynamic backgrounds,occlusion,and limited labeled *** address these challenges,we introduce a comprehensive methodology toenhance image classification and object detection *** proposed approach involves the integration ofmultiple methods in a complementary *** process commences with the application of Gaussian filters tomitigate the impact of noise *** images are then processed for segmentation using fuzzy C-Meanssegmentation in parallel with saliency mapping techniques to find the most prominent *** Binary RobustIndependent Elementary features(BRIEf)characteristics are then extracted fromdata derived fromsaliency mapsand segmented *** precise object separation,Oriented fAST and Rotated BRIEf(ORB)algorithms *** Algorithms(GAs)are used to optimize Random forest classifier parameters which lead toimproved *** method stands out due to its comprehensive approach,adeptly addressing challengessuch as changing backdrops,occlusion,and limited labeled data concurrently.A significant enhancement hasbeen achieved by integrating Genetic Algorithms(GAs)to precisely optimize *** minor adjustmentnot only boosts the uniqueness of our system but also amplifies its overall *** proposed methodologyhas demonstrated notable classification accuracies of 90.9%and 89.0%on the challenging Corel-1k and MSRCdatasets,***,detection accuracies of 87.2%and 86.6%have been *** ourmethod performed well in both datasets it may face difficulties in real-world data especially where datasets havehighly complex *** these limitations,GAintegration for parameter optimization shows a notablestrength in enhancing the overall adaptability and performance of our system.

关键词： BRIEf features saliency map fuzzy c-means object detection object recognition

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Quantitative and Qualitative Responses of Hydroponic Tomato Production to Different Levels of Salinity

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1311-1323页

作者： Khalid A.Al-Gaadi ahmed M.Zeyada ElKamil Tola Abdullah M.Alhamdan Khalid A.M.ahmed Rangaswamy Madugundu Mohamed K.Edrris Department of Agricultural Engineering College of Food and Agriculture SciencesKing Saud UniversityRiyadh11451Saudi Arabia Precision Agriculture Research Chair Deanship of Scientific ResearchKing Saud UniversityRiyadh11451Saudi Arabia Chair of Dates Industry&Technology Deanship of Scientific ResearchKing Saud UniversityRiyadh11451Saudi Arabia Agricultural Research Centre Agricultural Engineering Research Institute(AEnRI)Giza12619Egypt

from economic and nutritional points of view,tomato is,historically,considered one of the most important *** signiﬁcant yield reduction,most commercial cultivars of tomato crops are sensitive to moderate levels of ***,high levels of salt stress can negatively affect the yield and quality of tomato ***,this study was conducted to evaluate the yield and fruit quality of three tomato cultivars(forester,Ghandowra-f1,and feisty-Red)cultivated hydroponically,under three different levels of nutrient solution *** of tomato fruits was performed based on quantity(number and weight of fruits,and total fruit yield),physical quality(color andﬁrmness),and chemical quality(refractometric index“Brix”,pH,EC,K^(+),Na^(+),and NO_(3)-).Experiments were conducted using three salinity levels of the nutrient solution with electrical conductivity values(dSm^(-1))of 2.5(control),6.0,and *** showed that the studied tomato cultivars were signiﬁcantly inﬂuenced by high salinity(9.5 dSm^(-1))in comparison to the low(2.5 dSm^(-1))and medium(6.0 dSm^(-1))levels of *** average,the highest fruit weight per plant of 1944.84 g and total fruit yield of 4.42 kgm^(-2) were observed at the low salinity level;however,no signiﬁcant differences were obtained in the two yield factors(single fruit weight and total fruit yield)for the low and medium salinity *** the other hand,a signiﬁcant reduction in tomato yield(31%)was associated with the high salinity level compared to the yield at low and medium salinity *** of physical quality parameters showed highly signiﬁcant differences among all salinity *** average,the maximum value of color change(1.72)was associated with the medium salinity level,and the maximum value ofﬁrmness(9.61 Ncm^(-1))was recorded at the high salinity *** levels and tomato cultivars introduced signiﬁcant differences in chemical quality parameters;however,no signiﬁcant differences in these

关键词： Quality cultivars yield color ﬁrmness brix

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