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XAANTAL2 （AGL14） Is an Important Component of the Complex Gene Regulatory Network that Underlies Arabidopsis Shoot Apical Meristem Transitions

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Molecular Plant 2015年第5期8卷 796-813页

作者： Rigoberto V. Perez-R.u.iz Berenice Garcia-Ponce Nayelli Marsch-Martinez Yamel Ugartechea-Chirino Mitzi Villajuana-Bonequi Stefan de Folter Eugenio Azpeitia Jose Davila-Velderrain David Cruz-Sanchez Adriana Garay-Arroyo Maria de la Paz Sanchez Juan M. Estevez-Palmas Elena R. Alvarez-Buylla Instituto de Ecologia Universidad Nacionat Autonoma de Moxico 3er Circuito Exterior s/no Junto al Jardin Bot~nico and Centro de Ciencias de la ComplejidadCiudad Universitaria Coyoacan 04510 Mexico D.F. Mexico Laboratorio Nacional de Genemica para la Biodiversidad (Langebio) Centro de Investigaci6n y de Estudios Avanzados del Instituto Polit~cnico Nacional(CINVESTAV-IPN) Km. 9.6 Carretera Irapuato - Leen AP 629 36821 Irapuato Guanajuato Mexico University of California 431 Koshland Hall Berkeley CA 94720 USA These authors contributed equally to this article. present address: Departamento de Biotecnologia y Biquimica Centro de )nvestigaci6n y de Estudios Avanzados del Instituto Polit6cnico Nacional(CINVESTAV-IPN) Km. 9.6 Libramiento Norte Carr. Irapuato-Leen 36821 Irapuato Guanajuato Mexico present address: Max Planck Institute for Plant Breeding Research D-50829 Cologne Germany Present address: INRIA Project-Team Virtual Plants/CIRAD/INRA UMR AGAP Campus St Priest - BAT 5 CC 05018 860 rue de St Priest 34095 Montpellier Cedex5 France

In Arabidopsis thaliana, multiple genes involved in shoot apical meristem （SAM） transitions have been char- acterized, but the mechanisms required for the dynamic attainment of vegetative, inflorescence, and floral meristem （VM, IM, FM） cell fates during SAM transitions are not well understood. Here we show that a MADS-box gene, XAANTAL2 （XAL2/AGL14）, is necessary and sufficient to induce flowering, and its regula- tion is important in FM maintenance and determinacy, xal2 mutants are late flowering, particularly under short-day （SD） condition, while XAL2 overexpressing plants are early flowering, but their flowers have vege- tative traits. Interestingly, inflorescences of the latter plants have higher expression levels of LFY, AP1, and TFL1 than wild-type plants. In addition we found that XAL2 is able to bind the TFL1 regulatory regions. On the other hand, the basipetal carpels of the 35S：：XAL2 lines lose determinacy and maintain high levels of WUS expression under SD condition. To provide a mechanistic explanation for the complex roles of XAL2 in SAM transitions and the apparently paradoxical phenotypes of XAL2 and other MADS-box （SOCl, AGL24） over- expressors, we conducted dynamic gene regulatory network （GRN） and epigenetic landscape modeling. We uncovered a GRN module that underlies VM, IM, and FM gene configurations and transition patterns in wild- type plants as well as loss and gain of function lines characterized here and previously. Our approach thus provides a novel mechanistic framework for understanding the complex basis of SAM development.

关键词： XAL2/AGL14 MADS-box TFL1 SAM transitions floral reversion gene regulatory networks,epigenetic landscape modeling

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基于情景模拟游戏的家居能源消耗反弹效应测度研究

基于情景模拟游戏的家居能源消耗反弹效应测度研究

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作者：奥斯卡（Oscar garay）哈尔滨工业大学

学位级别：硕士

在过去的几十年中,全世界的制定者和管理者都开始制定政策以控制不断增长的能源消费问题。基于对能源耗尽和化石燃料价格上涨的考量,以及日益增强的大众环保意识,决策者们开始认真对待这个问题。为了解决这个问题,最常见的政策之一是提... 详细信息

在过去的几十年中,全世界的制定者和管理者都开始制定政策以控制不断增长的能源消费问题。基于对能源耗尽和化石燃料价格上涨的考量,以及日益增强的大众环保意识,决策者们开始认真对待这个问题。为了解决这个问题,最常见的政策之一是提高能源使用效率,例如,发电厂,输电和配电网以及各种家用电器。如果我们过分专注于这些政策,旨在减少家庭能源消耗,提高家用电器的能源效率,我们会遇到了一个问题:反弹效应。反弹效应是指随着能源效率的改善,但由于一些人为原因,节能效果明显低于预期的现象。当消费者认识到提高的能源效率可以降低自己日常的能源使用开销（即每月的能源费用）,消费者可能会不自觉地增加能量消耗。这种现象将极大程度上减少节能效果,最终导致节能策略达不到政策制定者的预期和要求。如果假定,提高能源效率可以降低能源价格,且能源的消耗量是与能源价格有弹性关系。那么合乎逻辑的结论是,由于反弹效应,提高能源使用效率有可能反而导致更高的消费。反弹效应已受到越来越多专家学者的关注和研究。虽然整个科学界一致认为,反弹效应是真实存在且可测的,但一些反弹效应的概念分歧一直存在。首先,如何衡量反弹效应的影响是一个问题。目前仍然没有一个被广泛接受的测量标准,不同专家学者对如何计算和测量反弹效应的效果仍存在分歧。不同的测量方法,标准和指标对反弹效应的研究过程造成影响。另一方面,一些其他学者声称,即使由于反弹效应的影响造成了一些能源节约损失,政策制定计划仍应尽可能多的调高能源消耗效率,以增加节约的能源消耗。这些反弹效应问题的发生主要有三个原因。首先,之前对于反弹效应影响的测量缺乏一个可靠有效的方法,在实际测量过程中经常出现测量方法错误。第二,不同的专家学者使用不同的测量标准和单位来衡量反弹效应。第三,以前对于反弹效应的分析采用了相对模糊和不同的研究范围。本论文研究的主要目的是寻找一种新的可靠方法来评估反弹效应的影响,以改善之前研究反弹效应过程中的相关问题。在处理这些问题的同时,反弹效应本身也会在一定程度上被缓解。在这项研究中,我们相信用一个新的方法情景模拟游戏来评估反弹的影响,可以解决一些产生争议的原因。情景模拟游戏是“一种以教育为主要目标而不是娱乐的游戏（游戏的形式多种多样）”。情景模拟游戏有很多优点。这些研究分析了之前尝试中出现的一些问题,过去的反弹效应的方法论问题也可以被解决。在这个复杂多变的背景下,本研究的总体目标是测试情景模拟游戏作为一种新的方法来评估和分析反弹效应的有效性,以改善以前评估方法的局限性。具体来说,本研究使用一个特定的情景模拟游戏进行一种全新的反弹效应评估,即“NRG游戏”（NRG是“能量”在英语中的缩写）。NRG游戏由荷兰代尔夫特理工大学的科技,政策与管理学院开发设计的。该游戏本来是用来测试消费者在不同外界干涉和条件下的行为（例如,能源价格下降,补贴可再生能源,电器折扣等）。在本研究中,NRG的游戏被定义为评估反弹效应在不同条件下对被测试者的影响。反弹效应评估模拟在自然环境中。在这种环境下,人们做出相应的能源消耗决定。例如:支付能源账单,买电器,卖电器,产生能量等。此外,NRG的游戏把主要注意力都放在对玩家行为的研究。让他们自由地来决定他们想要什么又需要什么。通过这种方式,NRG游戏研究了反弹效应的核心概念。其中包括了人的欲望,愿望,心理以及他们的能量消耗。根据这一评估的结果,情景模拟游戏在研究反弹效应的方面的有效性可以被测试出来。利用情景模拟游戏评估反弹效应是一个创新的方法。这种方法是第一次用于这种全新领域,可以给决策者一个新的角度来看待反弹效应。NRG游戏提供了一种新的反弹效应评估方法。具体来讲,50个被测试者被分为两个不同的组来执行前后行为分析:一组测试对象25人使用一个低效率的房子而另外一组25人使用一个高效率的房子。该游戏的样本量为50人,主要是生活在荷兰的成年人。研究样本的平均年龄为27岁,其中64%男,36%女。82%的被测对象至少是硕士学历以上。其中64%是在校学生其余36%已经工作。鉴于以上事实,该样本的选择在一定程度上限制了统计结果的代表性。但总体来讲,这两个群体至少具有一定可比性的,而结果具有较好的启示作用。通过进行测试前问卷调查的方式,每个测试对象要回答一些问题,以测试群体之间的可比性。结果显示这两个群体并没有表现出任何显着的差异,包括他们的环保态度,玩视频游戏的能力和频率,以及在现实中在家居能耗问题上的角色。如果两组之间在这四个标准检测中存在巨大差异,他们之间的可比性可能会被破坏。这种可比性的丧失将影响测试结果的最终质量。值得注意的是还有许多其他的标准可以被定义以检查这两个群体的可比性。但这四个标准被广泛认为是最重要的,以测试这种可比性。在实验中,每个被测试者会被随机分配到一个高或低效率的房子。他们有机会根据自己的意愿来管理他们的房子的能源消耗。被测试者主要需要考虑两个关键指式:1)房子的设计应尽可能符合自身兴趣,2)尽可

关键词：反弹效应能源使用效率情景模拟游戏前后行为分析家居节能

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Photocatalytic performance of K2Ti6O13 whiskers to H2 evolution and CO2 photo-reduction

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Journal of Energy Chemistry 2019年第10期28卷 18-28页

作者： L. F. garay-Rodríguez L.M.Torres-Martínez E.Moctezuma Facultad de Ingeniería Civil-Departamento de Ecomateriales y Energía Universidad Autónoma de Nuevo LeónAv. Universidad S/N Cd. UniversitariaC. P. 66455 San Nicolás de los GarzaNLMéxico Facultad de Ciencias Químicas Universidad Autónoma de San Luis PotosíAv. Manuel Nava#6C. P.78290 San Luis PotosíSLPMéxico

K2Ti6O13 whiskers were synthesized by conventional sol-gel method, sono-chemical assisted and microwave assisted sol-gel method in order to obtain catalysts with different particle sizes and to modify their optical, textural and electrochemical properties. These modifications improved their photocatalytic activity for H2 evolution and CO2 photo-reduction. Long K2Ti6O13 whiskers prepared by ultrasound assisted sol-gel method are the most active photocatalysts for the hydrogen evolution reaction using pure water as reactant (U-SG, 10,065 μmol g^-1). In contrast, an opposite behavior was observed using a mixture of ethanol-water, where the highest activity was achieved by the shortest and less crystalline K2Ti6O13 whiskers (C-SG, 3,2871 μmol g^-1). In case of CO2 photo-reduction, long whiskers that were also prepared by the sono-chemical assisted sol-gel method were the most active to transform CO2 to formaldehyde, methane, methanol and hydrogen. The EFB value of this catalyst is located very close to the potential for formaldehyde production and favors the selectivity to this organic product.

关键词： K2Ti6O13 Sol-gel Whiskers Hydrogen evolution CO2 reduction

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Bilateral diffuse grade 5 radiation pneumonitis after intensity modulated radiation therapy for localized lung cancer

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World Journal of Clinical Oncology 2017年第3期8卷 285-288页

作者： Virginia W Osborn Andrea Leaf Anna Lee Elizabeth garay Joseph Safdieh David Schwartz David Schreiber Veterans Affairs New York Harbor Healthcare System SUNY Downstate Medical Center

We are reporting a case of fatal radiation pneumonitis that developed six months following chemoradiation for limited stage small cell lung cancer.The patient was a 67-year-old man with a past medical history of Hashimoto's thyroiditis and remote suspicion for CREST,neither of which were active in the years leading up to treatment.He received 6600 cG y delivered in 200 cG y daily fractions via intensity modulated radiation therapy with concurrent cisplatin/etoposide followed by additional chemotherapy with dosereduced cisplatin/etoposide and carboplatin/etoposide and then received prophylactic cranial irradiation.The subsequent months were notable for progressively worsening episodes of respiratory compromise despite administration of prolonged steroids and he ultimately expired.Imaging demonstrated bilateral interstitial and airspace opacities.Autopsy findings were consistent with pneumonitis secondary to chemoradiation as well as lymphangitic spread of small cell carcinoma.The process was diffuse bilaterally although his radiation was delivered focally to the right lung and mediastinum.

关键词： Radiation Pneumonitis Small cell lung cancer Intensity modulated radiation therapy

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ALFREDO HARP HELú体育场

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当代建筑 2020年第6期 92-101页

作者： Francisco Gonzalez Pulido Alonso de garay Rafael Gamo(摄) 无不详 FGP Atelier and Taller ADG事务所

作为墨西哥首屈一指的棒球队,Los Diablos Rojos del Mexico在获得了16个锦标赛冠军和拥有70多年的历史之后,即将迎来震撼人心的、拥有13000多个遮阳座位和7000个护栏座位的新主场。2014年9月,团队负责人Alfredo HarpHelú先生委托建筑... 详细信息

作为墨西哥首屈一指的棒球队,Los Diablos Rojos del Mexico在获得了16个锦标赛冠军和拥有70多年的历史之后,即将迎来震撼人心的、拥有13000多个遮阳座位和7000个护栏座位的新主场。2014年9月,团队负责人Alfredo HarpHelú先生委托建筑师Alonso de garay(Taller ADG事务所)在Magdalena Mixhuca体育综合体内设计新的体育场。后者随即又邀请了建筑师Gonzàlez-Pulido(FGP Atelier事务所)进行五五分的设计合作,双方都认为这将是Diablos Rojos队所拥有的世界一流的体育设施。

关键词：体育设施体育场遮阳团队负责人事务所

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Efficient Synthesis of PbTe Nanoparticle Networks

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Nano Research 2010年第10期3卷 685-693页

作者： Qiangfeng Xiao Ding Weng Zhenglong Yang Javier garay Minjuan Zhang Yunfeng Lu Chemical and Biomolecular Engineering Department University of CaliforniaLos AngelesCA 90095USA Mechanical Engineering Department University of CaliforniaRiversideCA 92521USA Materials Research Department Toyota Motor Engineering and Manufacturing North AmericaInc.1555 Woodridge Ave.Ann ArborMI 48105USA

The synthesis of semiconductor nanocrystalline networks using weak capping ligands in aqueous media has been demonstrated.Carbohydrates,includingβ-cyclodextrin,D-(+)-glucose,D-glucosamine,lactobionic acid,sucrose,and starch were chosen as weak ligands to facilitate the formation of PbTe nanoparticle networks.The nanoparticle size,ranging from 5 nm to 30 nm,can be tuned by manipulating the temperature and concentration.Through a similar strategy,more complicated nanostructures including carbohydrate spheres@PbTe core-shell structures and Te@carbohydrate@PbTe multilayered submicron cables have been fabricated.This is a general approach which can be easily extended to the fabrication of other semiconductor networks,including PbSe and Bi2Te3 using carbohydrates and ethylenediaminetetraacetic acid(EDTA),respectively,as ligands.

关键词： Lead telluride nanoparticle network core shell structures thermoelectric carbohydrate

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STATISTICAL DETECTION OF SPATIAL PLANT PATTERNS UNDER THE EFFECT OF FOREST USE

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International Journal of Biomathematics 2012年第6期5卷 105-119页

作者： I. LoPEZ T. STANDOVAR J. garay Z. VARGA M. GAMEZ Department of Statistics and Applied Mathematics University of Almerla La Canada de San Urbano 04120 Almerla Spain Department of Plant Taxonomy and Ecology L. Eotvos University H-1117 Budapest Pazmany Peter setany 1lie. Hungary Research Group of Theoretical Biologyand Ecology of the Hungarian Academy of Sciences and Department of Plant Taxonomy and Ecology L. Eotvos University H-1117 Budapest Pazmany Peter setany 1//c. Hungary Institute of Mathematics and Informatics Szent Istvdn UniversityPdter K. u. 1 H-2103 Godollo Hungary Department of Statistics and Applied Mathematics University of AlmerlaLa Canada de San Urbano 05120 Almer~a Spain

The analysis of the consequences of land use (in particular forest use) may be considered as a partial step towards an integrated modeling of a land system. In the paper a forest territory is considered, where a gap-cut is made, and after a given time period the eventual change in the spatial distribution of undergrowth plants and tree seedlings is to be detected. Floristic data are collected along a line transect. A method for the detection of the change in the plant distributions along the transect is proposed to see whether this occurs at the geometric frontier of the human intervention. Since in the considered case the distribution of the change-point estimate is not known, as a substitute of its confidence interval, the so-called change-interval is calculated, using an adaptation of the bootstrap method. As an illustration, for a concrete plant species, the maximum likelihood estimation of the change-point and the calculation of the above mentioned change-interval is presented. Finally, the validation of the proposed method against some typical ecological situations is also presented, which provides a justification of the used algorithms. [ABSTRACT FROM AUTHOR]

关键词：统计检测森林利用空间分布种植模式最大似然估计林下植物建模分析土地制度

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Depression Intensity Classification from Tweets Using Fast Text Based Weighted Soft Voting Ensemble

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Computers, Materials & Continua 2024年第2期78卷 2047-2066页

作者： Muhammad Rizwan Muhammad Faheem Mushtaq Maryam Rafiq Arif Mehmood Isabel de la Torre Diez Monica Gracia Villar Helena garay Imran Ashraf Department of Artificial Intelligence The Islamia University of BahawalpurBahawalnagar63100Pakistan Department of Information Technology Khwaja Fareed University of Engineering and ITRYKhan64200Pakistan Department of Computer Science&Information Technology The Islamia University of BahawalpurBahawalnagar63100Pakistan Department of Signal Theory and Communications and Telematic Engineering Unviersity of ValladolidPaseo de Belén 15Valladolid47011Spain Faculty of Social Science and Humanities Universidad Europea del Atlántico Isabel Torres 21Santander39011Spain Department of Project Management Universidad Internacional IberoamericanaCampeche24560México Fundación Universitaria Internacional de Colombia Bogotá111611Colombia Universidad Internacional Iberoamericana AreciboPuerto Rico00613USA Universidade Internacional do Cuanza Cuito46703Angola Department of Information and Communication Engineering Yeungnam UniversityGyeongsan38541South Korea

Predicting depression intensity from microblogs and social media posts has numerous benefits and applications,including predicting early psychological disorders and stress in individuals or the general public.A major challenge in predicting depression using social media posts is that the existing studies do not focus on predicting the intensity of depression in social media texts but rather only perform the binary classification of depression and moreover noisy data makes it difficult to predict the true depression in the social media text.This study intends to begin by collecting relevant Tweets and generating a corpus of 210000 public tweets using Twitter public application programming interfaces(APIs).A strategy is devised to filter out only depression-related tweets by creating a list of relevant hashtags to reduce noise in the corpus.Furthermore,an algorithm is developed to annotate the data into three depression classes:‘Mild,’‘Moderate,’and‘Severe,’based on International Classification of Diseases-10(ICD-10)depression diagnostic criteria.Different baseline classifiers are applied to the annotated dataset to get a preliminary idea of classification performance on the corpus.Further FastText-based model is applied and fine-tuned with different preprocessing techniques and hyperparameter tuning to produce the tuned model,which significantly increases the depression classification performance to an 84%F1 score and 90%accuracy compared to baselines.Finally,a FastText-based weighted soft voting ensemble(WSVE)is proposed to boost the model’s performance by combining several other classifiers and assigning weights to individual models according to their individual performances.The proposed WSVE outperformed all baselines as well as FastText alone,with an F1 of 89%,5%higher than FastText alone,and an accuracy of 93%,3%higher than FastText alone.The proposed model better captures the contextual features of the relatively small sample class and aids in the detection of early depression intensity prediction from tweet

关键词： Depression classification deep learning FastText machine learning

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In silico Analysis of the Entire P. glaucum Genome Identifies Regulatory Genes of the bZIP Family Modulated in Response Pathways to Water Stress

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American Journal of Plant Sciences 2022年第2期13卷 277-293页

作者： Laura Beatriz garay Farías Sergio Litwiniuk Cristian Antonio Rojas Federal University of Latin American Integration (UNILA) Foz do Igua&#962 u Brazil National Council for Scientific and Technical Research of Argentina Posadas Argentina

The literature reviewed places P. glaucum as a cereal characterized by its nutritional quality and high tolerance to drought stress. However, very little is known about the fine mechanism it uses in response to water stress. To try to clarify this point, we carried out an analysis of the modulation of the expression of regulatory genes of the FT bZIP family. A full genome screening of P. glaucum identified 52 putative FT bZIPs, identifying 9 FT PgbZIP differentially expressed under water stress conditions filtered from RNA-seq data from a Transcriptome deposited at the NCBI. The promoter regions of these genes presented multiple elements or cis ABREs and DRE motifs, thus suggesting their double modulated participation in the slow or adaptive response and in the rapid response of this cereal to water stress. The findings of this study provide complementary data for the understanding of the mechanism behind the adaptation of P. glaucum under water stress, and may be relevant for molecular applications of potential crops.

关键词： P. glaucum Water Stress RNA-seq cis Elements FT PgBZIP

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Commitment of human mesenchymal stromal cells to skeletal lineages is independent of their morphogenetic capacity

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World Journal of Stem Cells 2023年第7期15卷 701-712页

作者： Jessica Cristina Marín-Llera Damián García-García Estefania garay-Pacheco Victor Adrian Cortes-Morales Juan Jose Montesinos-Montesinos Jesus Chimal-Monroy Medicina Genómica y Toxicología Ambiental Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCoyoacan 04510Mexico Laboratorio de Células Troncales Mesenquimales Unidad de Investigación Médica en Enfermedades OncológicasHospital de Oncología Centro Medico Nacional Siglo XXIInstituto Mexicano del Seguro SocialMexico City 06720Mexico Departamento de Medicina Genómica y Toxicología Ambiental Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCoyoacan 04510Mexico

BACKGROUND Mesenchymal stromal cells(MSCs)are multipotent cell populations obtained from fetal and adult tissues.They share some characteristics with limb bud mesodermal cells such as differentiation potential into osteogenic,chondrogenic,and tenogenic lineages and an embryonic mesodermal origin.Although MSCs differentiate into skeletal-related lineages in vitro,they have not been shown to selforganize into complex skeletal structures or connective tissues,as in the limb.In this work,we demonstrate that the expression of molecular markers to commit MSCs to skeletal lineages is not sufficient to generate skeletal elements in vivo.AIM To evaluate the potential of MSCs to differentiate into skeletal lineages and generate complex skeletal structures using the recombinant limb(RL)system.METHODS We used the experimental system of RLs from dissociated-reaggregated human placenta(PL)and umbilical cord blood(UCB)MSCs.After being harvested and reaggregated in a pellet,cultured cells were introduced into an ectodermal cover obtained from an early chicken limb bud.Next,this filled ectoderm was grafted into the back of a donor chick embryo.Under these conditions,the cells received and responded to the ectoderm’s embryonic signals in a spatiotemporal manner to differentiate and pattern into skeletal elements.Their response to differentiation and morphogenetic signals was evaluated by quantitative poly-merase chain reaction,histology,immunofluorescence,scanning electron microscopy,and in situ hybridization.RESULTS We found that human PL-MSCs and UCB-MSCs constituting the RLs expressed chondrogenic,osteogenic,and tenogenic molecular markers while differentially committing into limb lineages but could not generate complex structures in vivo.MSCs-RL from PL or UCB were committed early to chondrogenic lineage.Nevertheless,the UCB-RL osteogenic commitment was favored,although preferentially to a tenogenic cell fate.These findings suggest that the commitment of MSCs to differentiate into skeletal lineages differs according to the sou

关键词： Human mesenchymal stromal cells Recombinant limbs Mesenchymal stromal cell morphogene-sis Mesenchymal stromal cell in vivo differentiation Skeletal tissues

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