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Encapsulation of Immature Somatic Embryos of Coffea arabica *** in Vitro Preservation

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Phyton-International Journal of Experimental Botany 2021年第6期90卷 1741-1748页

作者： Eliana Arias-Pérez Carlos Alberto Lecona-Guzmán Federico Antonio Gutiérrez-Miceli Joaquín adolfo Montes-Molina Nancy Ruiz-Lau Laboratorio de Biotecnología Vegetal Instituto Tecnológico de Tuxtla GutiérrezTecnológico Nacional de MéxicoTuxtla GutiérrezChiapas29050México CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez Tuxtla GutiérrezChiapas29050México

The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos(Coffea arabica L.),for the conservation of genotypes with characteristics of commercial *** embryos were induced from leaf explants in Murashige and Skoog medium(MS)supplemented with 1 mg·L^(−1) of 2,4-dichlorophenoxiacetic acid(2,4-D)combined with 2 mg·L^(−1) of benzyladenine(BA).Somatic embryos(SE)at the globular stage were encapsulated in a sodium alginate matrix;two treatments were tested:MS+5 mg·L^(−1) BA+1 mg·L^(−1) NAA+3%(w/v)alginate,and MS+7 mg·L^(−1) BA+5.7 mg·L^(−1) indoleacetic acid(IAA)+3%(w/v)*** was complexed with 100 mM calcium chloride(CaCl_(2)).Viability of the encapsulated SE was determined by staining with 0.01%fluorescein diacetate(FDA)after 0,15,30,and 45 days of storage at 4℃.Embryo viability was 100%in both treatments.

关键词： Coffee explants embryo artificial seeds

Massive Vesical Hernias and Its Management: Case Series and Literature Review

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Open Journal of Urology 2022年第3期12卷 193-202页

作者： adolfo Castro-Rosas Juan Carlos Angulo-Lozano Nezahualcoyotl Gonzaga-Carlos Maria Fernanda Virgen-Rivera Mariel Gonzalez-Calatayud Jose Francisco Virgen-Gutiérrez Jorge Jaspersen-Gastelum Urology Department of Mexico General Hospital “Dr. Eduardo Liceaga” Mexico City Mexico School of Medicine Anahuac University Mexico City Mexico

Background: Bladder hernias are infrequent and should be treated by the urologist, or an experienced surgeon. An inguinoscrotal hernia of the bladder should be suspected in male patients with voiding symptoms and a fluctuant mass in the scrotum that changes its volume during urination. The most common characteristics found on the series were patients with advanced age, sedentary, and overweight. Case Presentation: We present a case series of 4 patients with inguinoscrotal herniation of the bladder, the surgical management, and outcome. Conclusion: The surgical technique used was inguinal plasty with Lichtenstein technique and either laparoscopic or open surgery mesh-plug placement. None had surgical complications, and none had recurrence at their follow-up.

关键词： Inguinal Plasty Bladder Herniation Lichtenstein Technique Functional Urology

Synchronous isolated splenic metastasis from colon carcinoma and concomitant splenic abscess:A case report and review of the literature

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World Journal of Gastroenterology 2007年第41期13卷 5516-5520页

作者： adolfo Pisanu Alberto Ravarino Riccardo Nieddu Alessandro Uccheddu Department of Surgery Department of Pathology University of CagliariClinica ChirurgicaCagliariItaly

This study aimed to describe a case in which an isolated splenic metastasis was synchronous with the colonic primary and a concomitant splenic abscess was associated. A wide review of the literature was also performed. A 54-year-old woman with abdominal pain and fever was admitted to our department. Abdominal CT revealed two low-density areas in the spleen and wall-thickening of the left colonic flexure,which was indistinguishable from the spleen parenchyma. The patient underwent emergency celiotomy,with the presumptive diagnosis of obstructing colon carcinoma of the splenic flexure,and concomitant splenic abscess. Subtotal colectomy and splenectomy were performed. Pathological findings were consistent with mucinous colonic carcinoma,synchronous isolated splenic metastasis and concomitant splenic abscess. This paper is also a review of the existing literature on the association between colorectal cancer and splenic metastasis. Only 41 cases of isolated splenic metastasis from colon carcinoma have been reported in the literature. This report is the third described case of synchronous isolated splenic metastasis from colon carcinoma. Only one case with concomitant splenic abscess has been previously reported. When obstructing left-sided colorectal cancer is suspected,careful CT examination can allow early diagnosis of splenic involvement by the tumor. The literature review suggests that there might be a significant improvement in survival following splenectomy for a metachronous isolated splenic metastasis from colon carcinoma. Prognosis for synchronous splenic metastasis seems to be related to the advanced stage of the disease. Nevertheless,no definitive conclusions can be drawn because of the small number of cases.

关键词：结肠癌脾肿脾转移症状

Recent advances in bird sperm morphometric analysis and its role in male gamete characterization and reproduction technologies

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Asian Journal of Andrology 2016年第6期18卷 882-888页

作者： Julian Santiago-Moreno Milagros Cristina Esteso Silvia Villaverde-Morcillo adolfo Toledano-Diaz Cristina Castafio Rosario Velazquez Antonio Lopez-Sebastian Agustin Lopez Goya Javier Gimeno Martinez Department of Animal Reproduction INIA 28040 Madrid Spain Veterinary Clinical and Research Services Fieb Foundation 28760 Tres Cantos Madrid Spain FAUNIA 28032 Madrid Spain

Postcopulatory sexual selection through sperm competition may be an important evolutionary force affecting many reproductive traits, including sperm morphometrics. Environmental factors such as pollutants, pesticides, and climate change may affect different sperm traits, and thus reproduction, in sensitive bird species. Many sperm-handling processes used in assisted reproductive techniques may also affect the size of sperm cells. The accurately measured dimensions of sperm cell structures （especially the head） can thus be used as indicators of environmental influences, in improving our understanding of reproductive and evolutionary strategies, and for optimizing assisted reproductive techniques （e.g., sperm cryopreservation） for use with birds. Computer-assisted sperm morphometry analysis （CASA-Morph） provides an accurate and reliable method for assessing sperm morphometry, reducing the problem of subjectivity associated with human visual assessment. Computerized systems have been standardized for use with semen from different mammalian species. Avian spermatozoa, however, are filiform, limiting their analysis with such systems, which were developed to examine the approximately spherical heads of mammalian sperm cells. To help overcome this, the standardization of staining techniques to be used in compute^-assessed light microscopical methods is a priority. The present review discusses these points and describes the sperm morphometric characteristics of several wild and domestic bird species.

关键词： avian computer-assisted assay morphometry spermatozoa

Cardiopulmonary Stability on a Patient with Hip Fracture and Severe Pulmonary Hypertension, Anesthetized with Lumbar-Sacral Plexus Block and Non-Invasive Ventilation: Case Report

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Open Journal of Anesthesiology 2022年第10期12卷 301-314页

作者： Uribe Campo Giselle Andrea Perales Caldera Eduardo Prol Carreiro adolfo Velazco González Jose Gamaliel Díaz Borjón Efraín Morales Maldonado Rubén Alejandro Department of Anesthesiology Hospital ángeles Lomas México City Mexico Department of Intensive Care Hospital ángeles Lomas México City Mexico Department of Orthopaedics Hospital ángeles Lomas México City Mexico

Background: Pulmonary hypertension is defined as a mean arterial pressure in the pulmonary artery exceeding 20 mm Hg at rest, measured by means of right heart catheterization. Patients with pulmonary hypertension undergoing surgical procedures such as hemiarthroplasty present a high risk of fatal complications. Nonetheless, there are no widely accepted protocols explaining their perioperative care in great detail. Case presentation: We present a case with an 89-years-old patient, with comorbidities such as GOLD 4D chronic obstructive pulmonary disease (COPD) and, as a consequence of this, severe pulmonary hypertension with signs of right ventricular dysfunction, thus requiring of pulmonary vasodilator, that has suffered a subcapital hip fracture requiring urgent surgery. Surgery is carried out successfully, under regional lumbar-sacral plexus block and sedation assisted by non-invasive ventilation. Conclusion: Multidisciplinary specialized treatment, preoperative optimization, as well as the careful selection of both the surgical and anesthetic techniques to be used, are among the strategies that improve the perioperative outcome in patients with pulmonary hypertension with right ventricle systolic dysfunction. Regional lumbar-sacral plexus block plus sedation is a technique that maintains hemodynamic stability;however, these patients require advance measures and postoperative monitoring under intensive care.

关键词： Hip Fracture Pulmonary Hypertension Lumbar-Sacral Plexus Block Non-Invasive Ventilation Right Ventricular Failure

汇率波动同美国分部门进口贸易流量之间关系研究

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汇率波动同美国分部门进口贸易流量之间关系研究

作者： GARCIA CORTES adolfo 复旦大学

学位级别：硕士

本文研究汇率水平及汇率波动对美国分部门进口贸易流量的影响。本文选取2002年1月至2012年12月之间美国同加拿大、中国、德国、日本及墨西哥等国的相关数据来进行上述研究。本文主要采用协整方法及误差纠正模型来研究美国分部门进口贸... 详细信息

本文研究汇率水平及汇率波动对美国分部门进口贸易流量的影响。本文选取2002年1月至2012年12月之间美国同加拿大、中国、德国、日本及墨西哥等国的相关数据来进行上述研究。本文主要采用协整方法及误差纠正模型来研究美国分部门进口贸易流量与其影响因素之间的关系。在研究过程中所使用的汇率波动为动态标准偏差,且实证过程中所用汇率为实际汇率。本文研究结果表明,汇率波动仅对美国对中国的分部门进口贸易流量产生显著正向影响。

关键词：汇率波动美国协整方法

Priorities and needs for research on urban interventions targeting vector-borne diseases: rapid review of scoping and systematic reviews

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Infectious Diseases of Poverty 2016年第1期5卷 962-967页

作者： Clara Bermudez-Tamayo Olive Mukamana Mabel Carabali Lyda Osorio Florence Fournet Kounbobr Roch Dabiré Celina Turchi Marteli adolfo Contreras Valéry Ridde Andalusian School of Public Health Campus de la Cartuja s/nGranada 18010Spain CIBERESP–Ciber de Epidemiología y Salud Pública Av.Monforte de Lemos3-5.Pabellón 11.Planta 0Madrid 28029Spain CHU Sainte-Justine 3175Chemin de la Côte-Sainte-CatherineH3T 1C4 MontrealQCCanada McGill University 845 Sherbrooke Street WestH3A 0G4 MontrealQCCanada Universidad del Valle(UDV) Cl.13#100-00CaliValle del CaucaColombia IRD Research Unit:MIVEGEC(Maladies infectieuses et vecteurs:écologiegénétiqueévolution et contrôle)911avenue AgropolisMontpellierFrance IRSS Bobo-Dioulasso399Avenue de la LibertéDirection Régionale de l’OuestBobo-DioulassoBurkina Faso Centro de Pesquisa Aggeu Magalhâes–Fiocruz/Pernambuco Av.Professor Moraes Regos/nCampus da UFPECidade UniversitáriaCEP:50.740-465 RecifePEBrazil University of Montreal Public Health Research Institute(IRSPUM) 7101Avenue du Parc.Montreal H3N1X9QuebecCanada Department of Social and Preventive Medicine School of Public HealthUniversity of Montreal7101Avenue du Parc.Montreal H3N1X9QuebecCanada

This paper highlights the critical importance of evidence on vector-borne diseases(VBD)prevention and control interventions in urban settings when assessing current and future needs,with a view to setting policy priorities that promote inclusive and equitable urban health *** should produce knowledge about policies and interventions that are intended to control and prevent VBDs at the population level and to reduce *** interventions include policy,program,and resource distribution approaches that address the social determinants of health and exert influence at organizational and system levels.

关键词： Vector-borne diseases Urban health Intervention

Strategies for MSME’s When Facing Pandemic Event: A Research of Restaurants in Tlaxcala during the COVID-19 Outbreak

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Strategies for MSME’s When Facing Pandemic Event: A Researc...

作者： Jesus adolfo Rodriguez Lozano 北京第二外国语学院

学位级别：硕士

Near to 95% of Mexican restaurants had to close during the quarantine(（Mendoza,2020）meanwhile65% of restaurants in Mexico will ‘die’ or have ‘high risk of bankruptcy’ due to,directly affecting two million 187 thousand jobs coronavirus（Forbes,2020）and,with an accelerated spreading of coronavirus,the sector has seen an unprecedented drop in demand because of the progressively loss of confidence in the *** Tlaxcala the industry was also severely affected,and it has been difficult for restaurants in this region to face the crisis without a certain way to face it,strategically *** paper argues that micro,small and medium enterprise（MSME’s）owners and managers could aim for a more proactive approach to incorporate strategies that be helpful to counter the negative effects caused by the crisis caused by the Covid-19 pandemic in the restaurant *** carry out the study,statistical data on Tlaxcala’s city restaurants was retrieved and analyzed in order to verify if the business owners and managers of these eateries were aware of strategies recommended for crisis,especially crisis management,business continuity plan and business process ***,semi-structured interviews were applied with these subjects to identify similarities between the theory of these strategic management concepts and the strategies taken during the crisis in order to visualize the potential opportunities of incorporating these concepts to their operativity,while also incorporating to these study successful procedures used by these businesses that could help other restaurants during these critical *** findings of these research show that there is room for improvement in the restaurant of Tlaxcala when it comes to strategies related to endure the crisis and,while chain restaurants possess a more structured plan to cope against the negative effects of the pandemic due to the assistance of their respective corporates,a small part of independent restaurants shown th

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非裔厄瓜多尔文学中的黑人性——对阿达贝托·奥尔蒂斯小说《朱云戈》的批判性跨文化分析

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非裔厄瓜多尔文学中的黑人性——对阿达贝托·奥尔蒂斯小说《朱云...

作者： Licoa Campos adolfo Fabricio 上海外国语大学

学位级别：硕士

阿达伯托·奥尔蒂斯是厄瓜多尔最伟大的作家之一,他的作品也是拉丁美洲非裔西班牙文学的杰出代表。作为黑白混血儿,他在两种文化和身份之间成长,切身体会了种族主义和阶级歧视。在写作中,他重现了厄瓜多尔的黑人性及其演变,特别是他的... 详细信息

阿达伯托·奥尔蒂斯是厄瓜多尔最伟大的作家之一,他的作品也是拉丁美洲非裔西班牙文学的杰出代表。作为黑白混血儿,他在两种文化和身份之间成长,切身体会了种族主义和阶级歧视。在写作中,他重现了厄瓜多尔的黑人性及其演变,特别是他的小说《朱云戈》。该小说获得了1942年厄瓜多尔“国家最佳小说奖”,讲述了大约三十年来非裔厄瓜多尔人的生活以及其与不同种族的关系。本文运用跨文化交际批评理论,分析了小说《朱云戈》中的权力关系如何影响跨文化交流以及非裔厄瓜多尔黑人性的建构、觉醒和解读。基于此,本文提出厄瓜多尔黑人性是一个长期的文化适应、转型和丰富的过程,是跨文化接触受到权力的种族、政治和社会结构影响的结果。本文还认为,权力差异导致了从属关系、种族歧视和社会不公,厄瓜多尔黑人性是对此的一种抵制。该论文得出的结论是,厄瓜多尔黑人性是一种极为复杂的文化建构,它来自受权力结构影响的跨文化人际交往中,原始非洲文化被修改的过程。最后本论文认为,非裔厄瓜多尔人通过保护非洲遗产,珍重并维护了他们的黑人性。

关键词：厄瓜多尔文学中的黑人性流散非州性阿达伯托·奥尔蒂斯《朱云戈》跨文化交际批评