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Somatostatin 3 loss of function impairs the innate immune response to intestinal inflammation

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Aquaculture and Fisheries 2021年第6期6卷 548-557页

作者： Jing ma Jie Chen Bruno Louro Rute S.T.martins adelino v.m.canario International Research Center for marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China CCmAR-Centre of marine Sciences University of the AlgarveGambelas Campus8005-139FaroPortugal

Somatostatin is a neuropeptide and a key regulator of the growth *** Sst encoding genes(sst1 to sst6)have been identified in teleost fish genomes but little is known about their *** present study aimed at replicating the context of the inflammatory bowel disease(IBD)and clarifying the involvement of sst3 in the intestine innate defence barrier in zebrafish *** first established a CRISP/Cas9 sst3 deficient line(mT)and analysed the morphological and transcriptomic response to 0.4%dextran sulfate sodium(DSS).Alcian blue staining of larval sections 6 days post fertilization showed an increased in acidic mucins in the intestinal bulb and mid-intestine,with a much stronger response in the mT compared to wild type(WT).The transcriptomic analysis revealed that WT and mT shared enriched gene ontology(GO)terms and pathways linked to catabolism,chondrocyte development,innate immune system,xenobiotic metabolism and oxidative *** contrast,the WT specific response to DSS was enriched in GO terms and pathways linked to transcription and translation,various developmental processes,regulation of biosynthetic processes,apelin signalling and apoptosis while the mT specific response included terms and pathways linked to protein metabolism and catabolic processes,extracellular matrix-receptor interaction and proteasome and chondrocyte ***,this study demonstrated that Sst3 deficiency impairs insulin growth factor and adipocytokine signalling exacerbating the inflammatory response to DSS.

关键词： Somatostatin 3 Zebrafish IBD Inflammation RNA-Seq

SuperSAGE digital expression analysis of differential growth rate in a European sea bass population

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Aquaculture and Fisheries 2019年第1期4卷 17-26页

作者： Bruno Louro Rute S.T.martins Patricia I.S.Pinto Richard Reinhardt Dirk-Jan de Koning adelino v.m.canario Deborah m.Power CCmAR-Centre of marine Sciences University of AlgarveCampus de GambelasFaro 8005-139Portugal max Planck Genome Centre Carl-von-Linne-Weg 10Köln 50829Germany The Roslin Institute and R(D)SvS University of EdinburghEaster BushMidlothian EH259RGScotland UK Department of Animal Breeding and Genetics Swedish University of Agricultural SciencesBox 7023Uppsala 75007Sweden

One of the goals of the aquaculture industry is to understand and control growth associated traits through selective *** the present study the molecular basis of growth heterogeneity in the European sea bass(Dicentrarchus labrax)was *** establish growth heterogeneity in a group of hatchery bred sea bass individuals were tagged and their specific growth rates(SGR)determined at monthly *** expression in the brain,liver and white muscle from fish with the most divergent sustained SGR(6 individuals of the first and last quartile)was assessed using SuperSAGE(Serial Analysis Gene Expression)combined with next generation SOLiD4 sequencing.A total of approx.11 million edited tags(26 bp),on average 2 million tags per SAGE library,that represented 47.071 unique transcripts were *** of transcripts in fish with high and low SGR yielded 344,698 and 601 differently expressed tags(0.01%false discovery rate and 4-fold change)in brain,liver and muscle,*** tags were mapped onto the sea bass genome and approximately one third of the tags could be assigned to annotated *** enrichment analysis revealed in liver,muscle and brain intricate gene expression changes in endocrine regulatory pathways involved in growth,metabolic and the stress axis,underlying divergent SGR in sea bass.

关键词： European sea bass Specific growth rate Gene expression SuperSAGE Pathways

somatostatin1基因突变斑马鱼仔鱼转录组分析

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上海海洋大学学报 2021年第5期30卷 777-788页

作者：赵文婷陈洁 LOURO Bruno 曹蕾马静 mARTINS Rute S.T. canario adelino v.m. 上海海洋大学国家海洋生物科学国际联合研究中心上海201306 CCmAR-Centre of marine Sciences University of the AlgarveGambelas CampusFaroPortugal 8005-139

利用CRISPR/Cas9基因编辑技术在斑马鱼中构建了可稳定遗传的生长抑素(somatostatin,SST)基因(sst1)突变体鱼系。通过比较6 dpf(days post fertilization,dpf)的sst1^(-/-)突变体和野生型仔鱼转录组,发现sst1^(-/-)突变体相关生化过程和信号通路发生了显著变化。相比野生型,sst1^(-/-)突变体仔鱼中有354个基因显著上调,504个基因显著下调。GO(gene ontology)富集分析表明,大部分差异基因与氨基酸和蛋白质生物合成相关。KEGG(kyoto encyclopedia of genes and genomes)通路富集显示代谢通路、氨基酸合成、氨基酰基-tRNA合成、核糖体合成和rRNA加工相关通路表达增强。结果表明,sst1^(-/-)突变体蛋白质生物合成和代谢过程更加活跃。RT-qPCR验证结果显示,随机选取的10个基因mRNA表达变化趋势与转录组测序结果一致,说明转录组测序结果真实可靠。6月龄突变体体质量、体长和野生型相比并无显著差异。本研究结果为进一步挖掘生长抑素基因家族的潜在功能奠定基础。

关键词： Somatostatin1 斑马鱼代谢蛋白质合成

Editorial:Aquaculture genomics

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Aquaculture and Fisheries 2019年第1期4卷 1-2页

作者： adelino v.m.Canário CCmAR-CImAR Centre of Marine SciencesUniversity of AlgarveCampus de GambelasFaro 8005-139Portugal International Research Centre for marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai 201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China

What can genomics offer to aquaculture and fisheries?The definition of Genomics,retrieval of biological meaning from large sets of DNA and/or RNA sequences present in organisms,highlights the potential benefits that can be accrued from this burgeoning *** continuous advances in sequencing technologies and bioinformatic tools,in particular in the last decade,enabled the harnessing of large amounts of genome-wide data from nonmodel organisms which can be used to address both fundamental and applied questions of fisheries,aquaculture and ecology(Abdelrahman et al.,2017).Genomics informed management is now considered absolutely essential if aquaculture and fisheries are to achieve their promise of making a significant contribution to sustainable food security,as the world demand for high quality protein continues to increase(Abdelrahman et al.,2017;Bernatchez et al.,2017;Yanez,Newman,&Houston,2015).However,although genomics approaches have become more common their practical use to improve fisheries management and aquaculture production still has not fulfilled its promise,despite overwhelming evidence of its advantages and benefits(Bernatchez et al.,2017;Garner et al.,2016;B.;Louro,Power,&canario,2014).Among the possible solutions to stimulate wider application of genomics knowledge are the need for greater communication among fishery scientists,geneticists,fishery managers,and also better communication of the potential of genomics approaches and the results to stakeholders by adoption of a clear and understandable dialogue(Bernatchez et al.,2017).While this is still a stumbling block that needs to be addressed in multiple contexts,it is equally important that more genomics-based papers are published in journals of a multidisciplinary nature such as Aquaculture and *** special issue is a small but a significant step towards communicating the power of genomics to a wide potential user community.

关键词： absolutely benefits promise

Sex steroids of black scabbardfish,Aphanopus carbo,in relation to reproductive and migratory dynamics

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Aquaculture and Fisheries 2021年第1期6卷 42-50页

作者： Inês Farias Elsa Couto Neide Lagarto Joāo Delgado adelino v.m.Canário Ivone Figueiredo Department of Sea and marine Resources Portuguese Institute for Sea and Atmosphere(IPMA)Avenida Doutor Alfredo Magalhāes Ramalho 6Algés1495-165Portugal mediterranean Institute for Advanced Studies(ImEDAE) Esporles07190Spain Centre of marine Sciences(CCmAR-CImAR) University of AlgarveCampus de GambelasFaro8005-139Portugal Regional Fisheries Directorate Research Service(DSI-DRP)Estrada da Pontinha S/NFunchal9004-562Portugal Interdisciplinary Centre of marine and Environmental Research(CIImAR-CImAR) Rua Dos BragasPorto4050-123Portugal

Black scabbardfish,Aphanopus carbo,is a commercially important species that takes distant migrations throughout its life *** steroids were measured by radioimmunoassay in the blood plasma of specimens caught off the madeira Archipelago and mainland Portugal to link this species migratory path with its reproductive ***,a pilot study using mozambique tilapia(Oreochromis mossambicus)was designed to evaluate the effect of sample freshness on steroid levels because black scabbardfish blood was collected at separate times after specimens were *** changes in T and 11-KT concentrations between the time of blood extraction and the time after preservation did not statistically differ among the different methods ***,measured black scabbardfish steroid concentrations were directly used in the subsequent data *** females,E2 and in T concentrations peaked at a late stage of vitellogenesis.E2 concentration was significantly different between females caught off each *** E2 and T concentrations from all developing females resulted in the separation of two distinct groups,independently of their geographical *** males,T and 11-KT were not significantly different between maturity *** hepatosomatic index of males caught off mainland Portugal was relatively *** may reflect a mechanism for storing energy that will later be consumed during migration to the spawning *** trend of sex steroids concentrations throughout the sexual maturation of the species is consistent with the morphological indicators and shows evidence of the reproductive and migratory pattern hypothesised for the black scabbardfish in NE Atlantic.

关键词： Black scabbardfish Deep-sea madeira mainland Portugal migration Sex steroids

Somatostatin 4 regulates growth and modulates gametogenesis in zebrafish

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Aquaculture and Fisheries 2019年第6期4卷 239-246页

作者： Chenchao Sui Jie Chen Jing ma Wenting Zhao adelino v.m.Canário Rute S.T.martins International Research Center for marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China CCmAR/CImAR Centre of marine Sciences University of the AlgarveGambelas Campus8005-139FaroPortugal

Somatostatin(SST)plays important roles in growth and *** teleost fishes six SST encoding genes(sst1 to sst6)have been identified although few studies have addressed their *** we aim to determine the function of the teleost specific sst4 in the zebrafish.A CRISPR/Cas9 sst4 zebrafish mutant with loss of function(sst4−/−)was produced which grew significantly faster and was heavier at the onset of gonadal maturation than the wild type(WT).Consistent with their faster growth,liver igf1,igf2a and igf2b expression was significantly upregulated in the sst4−/−fish compared to the *** examination of the ovaries and testis indicated that sst4−/−fish had slightly delayed testicular gametogenesis compared to the *** lower expression of igf3,amh,insl3,hsd17b3,hsd11b2,hsd20b,cyp11b and cyp17 was consistently observed in the sst4−/−*** contrast,the ovaries had lower expression of igf1,igf2a and cyp19a1a but increased expression of igf2b and *** gonadotrophin beta subunits(fshb and lhb)in the brain were downregulated indicating the brain-pituitary-gonadal axis was downregulated in the sst4−/−fish and suggesting that the steroid production is compromised in the maturing *** addition,analysis of sst1 and sst3 mRNA levels in sst4−/−fish suggests a dosage compensation effect of sst1 in the brain and ***,the results from the zebrafish sst4−/−line support the idea that sst4 is involved in the regulation of igf signalling,somatic growth and reproduction since steroidogenesis and gametogenesis at pubertal onset were compromised.

关键词： Somatostatin 4 Gametogenesis Zebrafish Gonadotrophin Puberty