SuperSAGE digital expression analysis of differential growth rate in a European sea bass population
作者机构：CCMAR-Centre of Marine SciencesUniversity of AlgarveCampus de GambelasFaro 8005-139Portugal Max Planck Genome CentreCarl-von-Linne-Weg 10Köln 50829Germany The Roslin Institute and R(D)SVSUniversity of EdinburghEaster BushMidlothian EH259RGScotland UK Department of Animal Breeding and GeneticsSwedish University of Agricultural SciencesBox 7023Uppsala 75007Sweden
出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)
年 卷 期：2019年第4卷第1期
页 面：17-26页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：The authors acknowledge funding by the European Commission of the European Union through the Network of Excellence Marine Genomics Europe(contract GOCE-CT-2004-505403) by the Portuguese Foundation for Science and Technology(FCT)through project CMAR/Multi/04326/2013 grants to BL(SFRH/BD/29171/2006) PISP(SFRH/BPD/25247/2005) RSTM(SFRH/BPD/66742/2009) BL benefited from a SABRETRAIN Marie Curie EST fellowship
主 题：European sea bass Specific growth rate Gene expression SuperSAGE Pathways
摘 要：One of the goals of the aquaculture industry is to understand and control growth associated traits through selective *** the present study the molecular basis of growth heterogeneity in the European sea bass(Dicentrarchus labrax)was *** establish growth heterogeneity in a group of hatchery bred sea bass individuals were tagged and their specific growth rates(SGR)determined at monthly *** expression in the brain,liver and white muscle from fish with the most divergent sustained SGR(6 individuals of the first and last quartile)was assessed using SuperSAGE(Serial Analysis Gene Expression)combined with next generation SOLiD4 sequencing.A total of approx.11 million edited tags(26 bp),on average 2 million tags per SAGE library,that represented 47.071 unique transcripts were *** of transcripts in fish with high and low SGR yielded 344,698 and 601 differently expressed tags(0.01%false discovery rate and 4-fold change)in brain,liver and muscle,*** tags were mapped onto the sea bass genome and approximately one third of the tags could be assigned to annotated *** enrichment analysis revealed in liver,muscle and brain intricate gene expression changes in endocrine regulatory pathways involved in growth,metabolic and the stress axis,underlying divergent SGR in sea bass.
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