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Introducing nitazoxanide as a promising alternative treatment for symptomatic to metronidazole-resistant giardiasis in clinical isolates

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Asian Pacific Journal of Tropical Medicine 2016年第9期9卷 866-871页

作者： Tahereh Mikaeili Galeh Abdolhassan Kazemi Mahmoud Mahami-Oskouei Behzad Baradaran adel spotin Seddigheh Sarafraz Majid Karamat Immunology Research Center Tabriz University of Medical Sciences Department of Parasitology and Mycology Faculty of MedicineTabriz University of Medical Sciences East Azerbaijan Province Health Center Tabriz Health Center

Objective:To identify the frequencies(F) of ferredoxin and nitroreductase mutations were identified on Iranian clinical isolates of Giardia lamblia in order to predict whether the nitazoxanide can be prescribed as suitable drug for symptomatic to metronidazoleresistant ***:Forty Giardia lamblia isolates as of 38 symptomatic and two metronidazole-resistant patients were collected from *** were extracted and amplified by targeting ferredoxin and Gl NR *** amplicons were directly sequenced to determine gene ***:The various amino acid substitutions(F:20%,Haplotype diversity:0.891,Tajima's D:-0.44013) were identified by analyzing ferredoxin gene in four symptomatic and two resistant ***,two haplotypes(F:5%,HD:0.345; Tajima's D:0.77815) characterized in metronidazole-resistant isolates of Gl NR,however,no point mutations was found in symptomatic ***:Non-synonymous mutations of ferredoxin oxidoreductase gene reduce translational regulatory protein's binding affinity which concludes reduction of ferredoxin expression and its *** leads to decrease in metronidazole drug delivery into the *** in these isolates may lead to their resistance to *** to low synonymous mutations of Gl NR demonstrates that nitazoxanide can be prescribed as promising alternative treatment for symptomatic to metronidazole-resistant giardiasis in Iranian clinical isolates.

关键词： Giardia lamblia Ferredoxin oxidoreductase Nitroreductase Nitazoxanide Metronidazole

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Phylogeography,genetic variability and structure of Acanthamoeba metapopulations in Iran inferred by 18S ribosomal RNA sequences:A systematic review and meta-analysis

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Asian Pacific Journal of Tropical Medicine 2017年第9期10卷 855-863页

作者： adel spotin Hamid Reza Moslemzadeh Mahmoud Mahami-Oskouei Ehsan Ahmadpour Maryam Niyyati Seyed Hossein Hejazi Fatemeh Memari Jafar Noori Immunology Research Center Tabriz University of Medical SciencesTabrizIran Department of Parasitology and Mycology Faculty of MedicineTabriz University of Medical SciencesTabrizIran Department of Medical Parasitology and Mycology School of MedicineShahid Beheshti University of Medical SciencesTehranIran Department of Parasitology and Mycology School of MedicineIsfahan University of Medical SciencesIsfahanIran

Objective: To verify phylogeography and genetic structure of Acanthamoeba populations among the Iranian clinical isolates and natural/artificial environments distributed in various regions of the ***: We searched electronic databases including Medline, Pub Med, Science Direct, Scopus and Google Scholar from 2005 to 2016. To explore the genetic variability of Acanthamoeba sp, 205 sequences were retrieved from keratitis patients, immunosuppressed cases and environmental sources as of various geographies of ***: T4 genotype was the predominant strain in Iran, and the rare genotypes belonged to T2, T3, T5(Acanthamoeba lenticulata), T6, T9, T11, T13 and T15(Acanthamoeba jacobsi).A total of 47 unique haplotypes of T4 were identified. A parsimonious network of the sequence haplotypes demonstrated star-like feature containing haplogroups IR6(34.1%) and IR7(31.2%) as the most common haplotypes. In accordance with the analysis of molecular variance, the high value of haplotype diversity(0.612–0.848) of Acanthamoeba T4 represented genetic variability within populations. Neutrality indices of the 18 S ribosomal RNA demonstrated negative values in all populations which represented a considerable divergence from neutrality. The majority of genetic diversity belonged to the infected contact lens and dust samples in immunodeficiency and ophthalmology wards, which indicated potential routes for exposure to a pathogenic Acanthamoeba sp. in at-risk individuals. A pairwise fixation index(FST) was from low to high values(0.024 33–0.418 92). The statistically FST points out that T4 is genetically differentiated between north-west, north-south and centralsouth metapopulations, but not differentiated between west-central, west-south, centralsouth, and north-central ***: An occurrence of IR6 and IR7 displays that possibly a gene flow of Acanthamoeba T4 occurred after the founder effect or bottleneck experience through ecological changes or host mobility

关键词： Acanthamoeba metapopulations Genetic variability Gene flow Molecular phylogeny Iran

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Microsporidiosis in Iran:A systematic review and meta-analysis

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Asian Pacific Journal of Tropical Medicine 2017年第4期10卷 322-330页

作者： Roghayeh Ghoyounchi Ehsan Ahmadpour adel spotin Mahmoud Mahami-Oskouei Azim Rezamand Nayyereh Aminisani Morteza Ghojazadeh Reza Berahmat Tahereh Mikaeili-Galeh Pediatric Health Research Center Tabriz University of Medical Sciences Department of Parasitology and Mycology Faculty of MedicineTabriz University of Medical Sciences Immunology Research Center Tabriz University of Medical Sciences Department of Pediatrics Faculty of MedicineTabriz University of Medical Sciences Department of Statistics and Epidemiology Faculty of Health SciencesTabriz University of Medical Sciences Research Center of Evidence Based Medicine Tabriz University of Medical Sciences

Objective:To examine all evidences about Microspondia infection in vertebrate/invertebrate hosts and Iranian populations distributed in different regions of the ***:All published articles up to December 2015,including descriptive and cross-sectional studies related to the prevalence and genotyping of Microspondia infection in Iran,was considered in this systematic *** meta-analysis was done using the random-effects model and Stats Direct statistical *** 5.05 software and maximum likelihood algorithm with Kimura 2-parameter model were used for phylogenetic ***:Of the 1 152 investigated studies,33 eligible studies reported a prevalence of Microspondia infection in vertebrate and invertebrate *** to this systematic review,the overall prevalence rate of Microsporidia infection in immunocompromised patients in Iran was 8.18%.Furthermore,the overall prevalence rate of Microsporidia infection in immunocompromised patients with chronic diarrhoea,patients with non-diarrhoea,gastroenteritis,and patients with CD4(< 200 cells/μL) was 15.4%,4.1%,0.5%,and 12.9%*** highest prevalence rate of human and animal Microsporidia was estimated in Kerman(29%) and Khuzestan(26.5%).The overall prevalence rate of Microsporidia infection in honeybees using the random-effects model was 40%.Furthermore,the highest prevalence rate of nosemosis was described in East Azerbaijan(48.2%).The maximum number of Microsporidia isolates from immunocompromised patients and pigeons in Iran belonged to genotypes D(n=16;50%) and E(n=6;20.6%) of Enterocytozoon ***:This study may be the first systematic review and meta-analysis that provides a broad outlook on the prevalence of microsporidiosis in *** is necessary to investigate Microsporidia infection in vertebrate and invertebrate hosts and environmental resources in Iran.

关键词： Microsporidia Microsporidiosis Genotypes Iran Prevalence Epidemiology

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Diagnosis of Toxoplasma gondii infection in pregnant women using automated chemiluminescence and quantitative real time PCR

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Asian Pacific Journal of Tropical Medicine 2019年第1期12卷 26-31页

作者： Ehsan Ahmadpour Elmira Zargami Mahmoud Mahami-Oskouei adel spotin Abbas Shahbazi Hossein Samadi Kafil Saba Rajabi Paria Alizadeh Yagoob Azadi Reza Bahaj Firouz Shahrivar Aleksandra Barac Infectious and Tropical Disease Research Center Tabriz University of Medical Sciences Students Research Committee Tabriz University of Medical Sciences Department of Parasitology and Mycology Faculty of Medicine Tabriz University of Medical Sciences Drug Applied Research Center Tabriz University of Medical Sciences Clinic for Infectious and Tropical Diseases Clinical Centre of Serbia Faculty of Medicine University of Belgrade

Objective: To identify serodiagnosis and quantification of Toxoplasma gondii(T. gondii) infection among pregnant women in Salmas, northwest of Iran. Methods: In this crosssectional study, 276 blood samples were collected from pregnant women referred to the health care centers in Salmas city. The demographic variables were also recorded. Titers of antiToxoplasma IgM and IgG antibodies(Ab) were determined using the chemiluminescence immunoassay. Quantitative real-time PCR targeting the T. gondii repeated element gene was also performed on the blood sample. Results: Out of all, 19.92%(55/276) and 2.17%(6/276) patients were seropositive for anti-Toxoplasma IgG and IgM Ab, respectively. Moreover, the presence of T. gondii DNA was observed in 12.31%(34/276) blood samples. A significant relationship was observed between the IgG Ab seropositivity and contact with the cat as a risk factor(P=0.022). Conclusions: The seroprevalence rate of T. gondii infection in pregnant women is relatively low. Consequently, the seronegative pregnant women are at risk, and a considerable rate of positive blood samples for the presence of parasite's DNA should not be ignored. Besides, quantitative real-time PCR could be considered as an accurate method for diagnosis of acute toxoplasmosis especially when the precise results are of the most importance in pregnancy. Limiting contact with cats is also suggested for pregnant women.

关键词： Toxoplasmosis Toxoplasma gondii Chemiluminescence immunoassay Real time PCR Pregnancy

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