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A simple,azulene-based colorimetric probe for the detection of nitrite in water

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Frontiers of chemical Science and Engineering 2020年第1期14卷 90-96,F0004页

作者： Lloyd c.Murfin carlos M.Lopez-Alled adam c.sedgwick Jannis Wenk Tony D.James Simon E.Lewis Department of chemistry University of BathBath BA27AYUK centre for Sustainable chemical Technologies University of BathBath BA27AYUK Department of chemical Engineering and Water Innovation and Research centre(WIRc@Bath) University of BathBath BA27AYUK

We describe the synthesis and evaluation of an azulene-based chemodosimeter for *** probe was found to undergo two distinct color changes upon introduction of aqueous nitrite ion.A near-instant formation of a grey color provides a qualitative indication of the presence of nitrite,followed by the formation of a deep-yellow/orange color,the endpoint from which quantitative data can be *** azulene probe exhibits 1:1 stoichiometry of reaction with nitrite in water,and is selective for nitrite over other *** azulene probe was applied to determine nitrite content in cured meat,and compared with the British Standard testing procedure(Griess test).The value obtained from the azulene-based probe agreed closely with the standard *** procedure only requires the preparation of one standard solution,instead of the three required for the standard Griess test.

关键词： azulene nitrite diazoquinone

Dual enzyme activated fluorescein based fluorescent probe

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Frontiers of chemical Science and Engineering 2020年第1期14卷 117-121页

作者： Maria L.Odyniec Jordan E.Gardiner adam c.sedgwick Xiao-Peng He Steven D.Bull Tony D.James Department of chemistry University of BathBathBA27AYUK Department of chemistry University of Texas at AustinAustinTX 78712-1224USA Key Laboratory for Advanced Materials&Feringa Nobel Prize Scientist Joint Research center East China University of Science and TechnologyShanghai 200237China

A simple dual analyte fluorescein-based probe(PF3-Glc)was synthesised containingβ-glucosidase(β-glc)and hydrogen peroxide(H2O2)trigger *** presence ofβ-glc,resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein(PF3).Subsequently,in the presence of glucose oxidase(GOx),the released glucose was catalytically converted to D-glucono-δ-lactone,which produced H2O2 as a *** GOx-produced H2O2,resulted in classic H2O2-mediated boronate oxidation and the release of the highly emissive fluorophore,*** unique cascade reaction lead to an 80-fold increase in fluorescence intensity.

关键词： chemosensors dual-activation GOx fluorescence β-glucosidase molecular logic

A boronic acid-based fluorescent hydrogel for monosaccharide detection

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Frontiers of chemical Science and Engineering 2020年第1期14卷 112-116页

作者： Suying Xu adam c.sedgwick Souad A.Elfeky Wenbo chen Ashley S.Jones George T.Williams A.Toby A.Jenkins Steven D.Bull John S.Fossey Tony D.James Department of Biochemistry Faculty of ScienceBeijing University of Chemical TechnologyBeijing 100029China Department of chemistry University of BathBath BA27AYUK Department of chemistry University of Texas at AustinTX 78712-1224USA School of chemistry University of BirminghamBirmingham B152TTUK National Institute of Laser Enhanced Sciences Cairo UniversityGiza 12613Egypt Higher Institute for Optics Technology SheratonCairo 17361Egypt Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power Shanghai University of Electric PowerShanghai 200090China

A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide *** solution,the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose,and an expected trend in apparent binding constants,as judged by a fluorescence response where D-fructose>D-galactose>D-mannose>*** hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of D-fructose resulting in a 10-fold enhancement(≤0.25 mol/L).

关键词： boron hydrogel monomer

Fluorescent probes for the detection of disease-associated biomarkers

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Science Bulletin 2022年第8期67卷 853-878,M0004页

作者： Wei-Tao Dou Hai-Hao Han adam c.sedgwick Guo-Biao Zhu Yi Zang Xin-Rong Yang Juyoung Yoon Tony D.James Jia Li Xiao-Peng He Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision chemistry and Molecular Engineering Feringa Nobel Prize Scientist Joint Research CenterFrontiers Center for Materiobiology and Dynamic ChemistrySchool of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghai 200237China National center for Drug Screening State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Department of Liver Surgery&Transplantation Liver Cancer InstituteZhongshan HospitalFudan UniversityKey Laboratory of Carcinogenesis and Cancer InvasionMinistry of EducationShanghai 200032China Department of chemistry University of BathBath BA27AYUK Department of chemistry and Nanoscience Ewha Womans UniversitySeoul 03760Republic of Korea Department of chemistry The University of Texas at AustinAustinTX 78712-1224USA School of chemistry and chemical Engineering Henan Normal UniversityXinxiang 453007China

Fluorescent probes have emerged as indispensable chemical tools to the field of chemical biology and *** ability to detect intracellular species and monitor physiological processes has not only advanced our knowledge in biology but has provided new approaches towards disease *** this review,we detail the design criteria and strategies for some recently reported fluorescent probes that can detect a wide range of biologically important species in cells and in *** doing so,we highlight the importance of each biological species and their role in biological systems and for disease *** then discuss the current problems and challenges of existing technologies and provide our perspective on the future directions of the research ***,we hope this review will provide inspiration for researchers and prove as useful guide for the development of the next generation of fluorescent probes.

关键词： Fluorescent probes Fluorescence imaging Biomarker detection chemical biology

2D-ultrathin MXene/DOXjade platform for iron chelation chemo-photothermal therapy

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Bioactive Materials 2022年第8期7卷 76-85页

作者： Yunjie Xu Yingwei Wang Jusung An adam c.sedgwick Mingle Li Jianlei Xie Weibin Hu Jianlong Kang Sajal Sen Axel Steinbrueck Bin Zhang Lijun Qiao Swelm Wageh Jonathan F.Arambula Liping Liu Han Zhang Jonathan L.Sessler Jong Seung Kim Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationShenzhen Institute of Translational MedicineDepartment of OtolaryngologyShenzhen Second People’s HospitalThe First Affiliated HospitalCollege of Optoelectronic EngineeringShenzhen UniversityShenzhen518060China Department of chemistry Korea UniversitySeoul02841South Korea Department of Hepatobiliary and Pancreatic Surgery Shenzhen People’s HospitalSecond Clinical Medical College of Jinan UniversityShenzhen518020China Department of chemistry The University of Texas at AustinAustinTX78712-1224USA Hunan Key Laboratory of Nanophotonics and Devices School of Physics and ElectronicsCentral South University932 South Lushan RoadChangsha410083China Institute of Translation Medicine Shenzhen Second People’s Hospital First Affiliated Hospital of Shenzhen University Shenzhen518035China Department of Physics Faculty of ScienceKing Abdulaziz UniversityJeddah21589Saudi Arabia

An increased demand for iron is a hallmark of cancer cells and is thought necessary to promote high cell proliferation,tumor progression and *** makes iron metabolism an attractive therapeutic ***,current iron-based therapeutic strategies often lack effectiveness and can elicit off-target *** report here a dual-therapeutic prodrug,DOXjade,that allows for iron chelation chemo-photothermal cancer *** prodrug takes advantage of the clinically approved iron chelator deferasirox(ExJade®)and the topoisomerase 2 inhibitor,doxorubicin(DOX).Loading DOXjade onto ultrathin 2D Ti_(3)c_(2) MXene nanosheets produces a construct,Ti_(3)c_(2)-PVP@DOXjade,that allows the iron chelation and chemotherapeutic functions of DOXjade to be photo-activated at the tumor sites,while potentiating a robust photothermal effect with photothermal conversion efficiencies of up to 40%.Antitumor mechanistic investigations reveal that upon activation,Ti_(3)c_(2)-PVP@DOXjade serves to promote apoptotic cell death and downregulate the iron depletion-induced iron transferrin receptor(TfR).A tumor pH-responsive iron chelation/photothermal/chemotherapy antitumor effect was achieved both in vitro and in *** results of this study highlight what may constitute a promising iron chelation-based phototherapeutic approach to cancer therapy.

关键词： 2D MXene Nanomedicine Prodrug Iron chelation Photothermal therapy

A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+breast cancer cells

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Microsystems & Nanoengineering 2024年第1期10卷 287-299页

作者： Braulio Andrés Ortega Quesada Jonathan cuccia Rachael coates Blake Nassar Ethan Littlefield Elizabeth c.Martin adam T.Melvin cain Department of chemical Engineering Louisiana State UniversityBaton RougeLA 70803USA Department of chemical and Biological Engineering Clemson UniversityClemsonSC 29634USA Biological and Agricultural Engineering Louisiana State UniversityBaton RougeLA 70803USA Department Medicine Section Hematology and Medical OncologyTulane UniversityNew OrleansLA 70118USA

Metastatic breast cancer leads to poor prognoses and worse outcomes in patients due to its invasive behavior and poor response to *** is still unclear what biophysical and biochemical factors drive this more aggressive phenotype in metastatic cancer;however recent studies have suggested that exposure to fluid shear stress in the vasculature could cause *** this study a modular microfluidic platform capable of mimicking the magnitude of fluid shear stress(FSS)found in human vasculature was designed and *** device provides a platform to evaluate the effects of FSS on McF-7 cell line,an estrogen receptor positive(ER+)breast cancer cell line,during circulation in the *** of the effects of FSS on McF-7 cells was carried out utilizing two approaches:single cell analysis and bulk *** single cell analysis,cells were trapped in a microarray after exiting the serpentine channel and followed by immunostaining on the device(on-chip).Bulk analysis was performed after cells were collected in a microtube at the outlet of the microfluidic serpentine channel for western blotting(off-chip).It was found that cells exposed to an FSS magnitude of 10 dyn/cm^(2) with a residence time of 60 s enhanced expression of the proliferation marker Ki67 in the McF-7 cell line at a single cell *** understand possible mechanisms for enhanced Ki67 expression,on-chip and off-chip analyses were performed for pro-growth and survival pathways ERK,AKT,and JAK/*** demonstrated that after shearing the cells phosphorylation of p-AKT,p-mTOR,and p-STAT3 were ***,there was no change in p-ERK1/*** is a mediator of ER rapid signaling,analysis of phosphorylated ERαwas carried out and no significant differences between sheared and non-sheared populations were *** together these results demonstrate that FSS can increase phosphorylation of proteins associated with a more aggressive phenotype in circulating cancer *** findin

关键词： fluid breast estrogen

Degradation and resynthesis of chlorophyll during increased oxidative stress and prolonged darkness differ between annual and perennial flax(Linum L.)

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Oil crop Science 2024年第2期9卷 121-130页

作者： Kenyon J. Nisbett Abida Alokozai Su Hyun Elizabeth Ko G. adam Mott Jason c.L. Browna Department of Biological Sciences University of Toronto ScarboroughTorontoONCanada Department of cell&Systems Biology University of TorontoTorontoONCanada centre for the Analysis of Genome Evolution&Function University of TorontoTorontoONCanada

Among plants,there is considerable variation in lifespan:annuals live less than one year,whereas perennials live for several years,with the longest-living perennial having survived 43,600 *** proposed by the Disposable Soma Theory,this lifespan variation among plants likely reflects differential investment of limited energy and nutrient resources,with perennials investing more energy and nutrients into biomolecular maintenance compared to annuals in order to ensure persistence over multiple *** differential investment may be particularly important during periods of exogenous stress,which are known to accelerate biomolecular *** present study evaluated this hypothesis using annual and perennial flax(Linum L.)subjected to two exogenous stressors—increased oxidative stress(i.e.,foliar H2O2spraying)and complete prolonged *** chlorophyll has been shown to exhibit degradation in response to changes in environmental conditions,we utilized changes in chlorophyll levels during and after periods of exogenous stress to evaluate our *** predicted that i)perennials would exhibit a slower rate of chlorophyll degradation during exposure to exogenous stressors compared to annuals,and ii)perennials would exhibit a faster rate of chlorophyll resynthesis following such exposure compared to *** levels before,during,and after exposure to both exogenous stressors were measured in two separate trails,once using image colour analysis and once using *** chlorophyll degradation rates in response to oxidative stress did not differ between annuals and perennials,contrary to our predictions,chlorophyll resynthesis rates following such exposure were significantly higher in perennials,as *** plants were subjected to complete prolonged darkness,chlorophyll degradation rates were significantly lower in perennials than annuals,as predicted;however,when plants were subsequently reintroduced to natural photoperiod,ch

关键词： Ageing Life history Maximum lifespan Oxidative stress Somatic maintenance

Multinuclear MR and MRI study of lithium-ion cells using a variable field magnet and a fixed frequency RF probe

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Magnetic Resonance Letters 2024年第1期4卷 10-20页

作者： Andrés Ramírez Aguilera Florin Marica Kevin J.Sanders Md Al Raihan c.adam Dyker Gillian R.Goward Bruce *** UNB MRI centre Department of PhysicsUniversity of New BrunswickFrederictonNew BrunswickE3B 5A3Canada Department of chemistry McMaster UniversityHamiltonOntarioL8S 4L8Canada Department of chemistry University of New BrunswickFrederictonNew BrunswickE3B 5A3Canada

An exploratory multinuclear magnetic resonance(MR)and magnetic resonance imaging(MRI)study was performed on lithium-ion battery cells with ^(7)Li,^(19)F,and ^(1)H measurements.A variable field superconducting magnet with a fixed frequency parallel-plate radiofrequency(RF)probe was employed in the *** magnetic field was changed to set the resonance frequency of each nucleus to the fixed RF probe frequency of 33.7 *** cartridge-like lithium-ion cells,with graphite anodes and LiNi_(0.5)Mn_(0.3)co_(0.2)O_(2)(NMc)cathodes,were *** cell was pristine,and one was charged to a cell voltage of 4.2 *** results presented demonstrate the great potential of the variable field magnet approach in multinuclear measurement of lithium-ion *** methods open the door for developing faster and simpler methods for detecting,quantifying,and interpreting MR and MRI data from lithium-ion and other batteries.

关键词： Multinuclear MR/MRI Variable field magnet Lithium-ion battery Parallel-plate rf probe

Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing

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cellular & Molecular Immunology 2024年第4期21卷 362-373页

作者： Zeguang Wu Qiezhong Lamao Meichao Gu Xuanxuan Jin Ying Liu Feng Tian Ying Yu Pengfei Yuan Shuaixin Gao Thomas S.Fulford adam P.Uldrich catherine c.L Wong Wensheng Wei Biomedical Pioneering Innovation center Peking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University100871BeijingChina EdiGene Inc. Life Science ParkChangping District102206BeijingChina center for Precision Medicine Multi-Omics Research Peking University Health Science CenterPeking University100191BeijingChina Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity The University of MelbourneParkvilleVIC3010Australia cancer Immunology Program Peter MacCallum Cancer CentreMelbourneVICAustralia State Key Laboratory for complex Severe and Rare DiseasesClinical Research InstitutePeking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegePeking-Tsinghua Center for Life Sciences100871BeijingChina

Vγ9Vδ2 T cells are specialized effector cells that have gained prominence as immunotherapy agents due to their ability to target and kill cells with altered pyrophosphate *** our effort to understand how cancer cells evade the cell-killing activity of Vγ9Vδ2 T cells,we performed a comprehensive genome-scale cRISPR screening of cancer *** found that four molecules belonging to the butyrophilin(BTN)family,specifically BTN2A1,BTN3A1,BTN3A2,and BTN3A3,are critically important and play unique,nonoverlapping roles in facilitating the destruction of cancer cells by primary Vγ9Vδ2 T *** coordinated function of these BTN molecules was driven by synchronized gene expression,which was regulated by IFN-γsignaling and the RFX ***,an enzyme called QPcTL was shown to play a key role in modifying the N-terminal glutamine of these BTN proteins and was found to be a crucial factor in Vγ9Vδ2 T cell killing of cancer *** our research,we offer a detailed overview of the functional genomic mechanisms that underlie how cancer cells escape Vγ9Vδ2 T ***,our findings shed light on the importance of the harmonized expression and function of gene family members in modulating T-cell activity.

关键词： Butyrophilin Vγ9Vδ2 T-cell cancer-specific immune evasion Glutaminyl-peptide cyclotransferase-like Pyrophosphate metabolite Immunotherapy