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DNA Binding and Antitumor Activity of a New Fluores cent ProbeEu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety

光谱学与光谱分析 2018年第11期38卷 3611-3621页

作者： Belal H M Hussein Mostafa A Gouda Omer Sakin abdulla faluji Mohamed Gomaa Hassan A Azab Walid Fathalla Sherin Arabi Sa wsan Mosa Chemistry Department Faculty of Science&ArtsAl UlaTaibah UniversityAl Madina Al MonawarahKSA Department of Chemistry Faculty of ScienceSuez Canal UniversityIsmailiaEgypt Chemistry Department Faculty of ScienceMansoura UniversityAl MansouEgypt Biotechnology Research Center Suez Canal Universitylsmailia 41522Egypt Medicinal Chemistry Department Faculty of PharmacySuez Canal Universitylsmailia 41522Egypt Department of Mathematical and Physical Sciences Faculty of EngineeringPort-Said UniversityPort-SaidEgypt Department of Mathematical and Physical Sciences Faculty of EngineeringSuez Canal UniversitylsmailiaEgypt Department of Chemistry Faculty of Educationel Arish UniversityEgypt

A new europium(Ⅲ)complex containing(4-Methyl-2-oxo-2 H-chromen-7-yloxy)-acetic acid moiety(CMMC)was synthesized,characterized,and confirmed as antitumor agent and fluorescent *** spectroscopic measurements of Eu(Ⅲ)in the presence of CMMC were obtained in different *** results show that the strongest Eu(Ⅲ)emission bands were monitored in iso-propyl alcohol while the weakest Eu(Ⅲ)emission band was observed in *** interaction of Eu(Ⅲ)-(CMMC)2 complex with DNA was monitored using absorption and emission *** fluorescence titration measurements,the binding constants of DNA with Eu(Ⅲ)-(CMMC)_2 complex were found to be 1.04×10~5 L·mol^(-1) in Tris-HCl and 1.17×10~7 L·mol^(-1) in DMSO-Tris-HCl buffer(9∶1 V/V).Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Eu(Ⅲ)-complex between the base pair of *** result further confirmed by fluorescent Ethidium bromide displacement *** fluorescence calibration curve was used for the determination of DNA with LOD of 1.2 ng in DMSO-Tris-HCl buffer(9∶1 V/V)and 5 ng in Tris-HCl *** preliminary antitumor investigation shows promising cytotoxicity against MDA-MB-231,MCF-7(mammary cancer),and PC-3(prostate carcinoma)cell lines with IC50 values of 40.63,25.42 and 30.25μmol·L^(-1),respectively.

关键词： Europium (4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid DNA sensing Spectroscopic properties Antitumor activ ity

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