DNA Binding and Antitumor Activity of a New Fluores cent ProbeEu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety

作     者：Belal H M Hussein Mostafa A Gouda Omer Sakin Abdulla Faluji Mohamed Gomaa Hassan A Azab Walid Fathalla Sherin Arabi Sa wsan Mosa Belal H M Hussein;Mostafa A Gouda;Omer Sakin;Abdulla Faluji;Mohamed Gomaa;Hassan A Azab;Walid Fathalla;Sherin Arabi;Sawsan Mosa

作者机构：Chemistry DepartmentFaculty of Science&ArtsAl UlaTaibah UniversityAl Madina Al MonawarahKSA Department of ChemistryFaculty of ScienceSuez Canal UniversityIsmailiaEgypt Chemistry DepartmentFaculty of ScienceMansoura UniversityAl MansouEgypt Biotechnology Research CenterSuez Canal Universitylsmailia 41522Egypt Medicinal Chemistry DepartmentFaculty of PharmacySuez Canal Universitylsmailia 41522Egypt Department of Mathematical and Physical SciencesFaculty of EngineeringPort-Said UniversityPort-SaidEgypt Department of Mathematical and Physical SciencesFaculty of EngineeringSuez Canal UniversitylsmailiaEgypt Department of ChemistryFaculty of Educationel Arish UniversityEgypt 

出 版 物：《光谱学与光谱分析》 (Spectroscopy and Spectral Analysis)

年 卷 期：2018年第38卷第11期

页      面：3611-3621页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants 4590/1434 by Deanship of Scientific Resea rch at Taibah University in Saudi Arabia for financial assistance 

主　　题：Europium (4-Methyl-2-oxo-2H-chromen-7-yloxy)-acetic acid DNA sensing Spectroscopic properties Antitumor activ ity 

摘      要：A new europium(Ⅲ)complex containing(4-Methyl-2-oxo-2 H-chromen-7-yloxy)-acetic acid moiety(CMMC)was synthesized,characterized,and confirmed as antitumor agent and fluorescent *** spectroscopic measurements of Eu(Ⅲ)in the presence of CMMC were obtained in different *** results show that the strongest Eu(Ⅲ)emission bands were monitored in iso-propyl alcohol while the weakest Eu(Ⅲ)emission band was observed in *** interaction of Eu(Ⅲ)-(CMMC)2 complex with DNA was monitored using absorption and emission *** fluorescence titration measurements,the binding constants of DNA with Eu(Ⅲ)-(CMMC)_2 complex were found to be 1.04×10~5 L·mol^(-1) in Tris-HCl and 1.17×10~7 L·mol^(-1) in DMSO-Tris-HCl buffer(9∶1 V/V).Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Eu(Ⅲ)-complex between the base pair of *** result further confirmed by fluorescent Ethidium bromide displacement *** fluorescence calibration curve was used for the determination of DNA with LOD of 1.2 ng in DMSO-Tris-HCl buffer(9∶1 V/V)and 5 ng in Tris-HCl *** preliminary antitumor investigation shows promising cytotoxicity against MDA-MB-231,MCF-7(mammary cancer),and PC-3(prostate carcinoma)cell lines with IC50 values of 40.63,25.42 and 30.25μmol·L^(-1),respectively.