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Insecticide susceptibility status and resistance mechanism of Anopheles cracens Sallum and Peyton and Anopheles maculatus Theobald(Family: Culicidae) from knowlesi malaria endemic areas in Peninsular Malaysia

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Asian Pacific Journal of Tropical Medicine 2019年第2期12卷 79-86页

作者： Ahmad Rohani abdul rahman aidil azahary Ke-Xin Yu Mohamed Nor Zurainee Wan Mohamad Ali Wan Najdah Han Lim Lee Medical Entomology Unit Institute for Medical Research(Malaysia) Department of Parasitology University of Malaya Faculty of Health and Life Sciences Management and Science University(Malaysia)

Objective: To assess the insecticide susceptibility status of Anopheles cracens(***) and Anopheles maculatus(***) from knowlesi malaria endemic areas in Peninsular Malaysia towards DDT, malathion and deltamethrin and to determine the resistance mechanism ***: Adult and larval mosquitos were collected for *** status of Anopheles was determined using the standard WHO adult bioassay, larval bioassay and biochemical enzyme ***: WHO adult bioassay results indicated *** collected from Kampung Sungai Ular, Pahang was resistant towards 4% DDT, while *** collected from Kampung Sokor, Kelantan and Kampung Sungai Lui, Selangor exhibited resistance towards 4% ***, the enzyme activity profiles varied according to strains and *** resistance ratio of larval bioassay, showed that all strains and species tested were susceptible to malathion and ***: Since only a few anopheline strains exhibited low level of insecticide resistance towards malathion, DDT and *** insecticides are still considered effective for vector control program towards *** and ***.

关键词： Insecticide susceptibility Vector control WHO adult bioassay Biochemical enzyme assay

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Comparative Human Landing Catch and CDC Light Trap in Mosquito Sampling in Knowlesi Malaria Endemic Areas in Peninsula Malaysia

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Advances in Entomology 2016年第1期4卷 1-10页

作者： Ahmad Rohani Abd rahman aidil azahary Mohamed Nor Zurainee Wan Mohd Ali Wan Najdah Ismail Zamree Mohd Omar Hanif Mohd Majid Ariffin Hamid Zuhaizam Ismail Suzilah Han Lim Lee Medical Entomology Unit Institute for Medical Research Kuala Lumpur Malaysia Department of Parasitology University of Malaya Kuala Lumpur Malaysia School of Quantitative Sciences (SQS) UUM College of Arts and Sciences University Utara Malaysia Sintok Malaysia

Adult mosquito sampling techniques are essential for monitoring transmission of malaria and other mosquito borne infections. Preference for any sampling technique depends on both its field efficiency and the characteristics of local vector populations. Surveys on adult mosquitoes using Human Landing Catch (HLC) and CO2-baited CDC light trap (CDC-LT) techniques were conducted in several knowlesi malaria endemic areas between the months of March to December 2012 in several states of Peninsula Malaysia. These two techniques were relatively compared to determine the preferences of anopheline mosquitoes towards CO2-baited CDC-LT technique using HLC technique as the reference method. Cx. gelidus, An. maculates and An. introlatus were the main three species collected by HLC technique, whereas the species collected by CO2-baited CDC-LT technique were mostly An. cracens, Ar. durhami and Coquillettidia species. Most of the Anopheles species were collected almost exclusively by the human collectors except for An. cracens and An. introlatus which were collected using both techniques. Anopheles cracens was the most dominant species captured using CO2-baited CDC-LT technique. This is the first report showing An. cracens was caught using CO2-baited CDC-LT technique in Malaysia.

关键词： Anopheles cracens Human Landing Catch CDC Light Trap

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