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Development of Starfruit Simple Sequence repeat (SSr) Using Next Generation Sequencing

Journal of Food Science and Engineering 2019年第3期9卷 95-121页

作者： Khairun Hisam Nasir Muhammad Fairuz Mohd Yusof Mohd Shahril Firdaus Siti Zainab Jantan Mira Farzana Mohamad Moktar Siti Norsaidah Ibrahim Noor Baiti abdul Aziz Joanna Cho Lee Ying Centre for Molecular Marker Discovery and Validation (CMDV) Agri-Omic dan Bioinformatics Biotechnology and Nanotechnology Research Centre Malaysian Agricultural Research and Development Institute (MARDI) 43400 Serdang Selangor Malaysia Horticulture research Centre Headquarters Malaysian Agricultural Research and Development Institute (MARDI) 43400 Serdang Selangor Malaysia

Starfruit (Averrhoa carambola L.) is an important fruit for Malaysian export and great attention has been made to improve starfruit fruit quality at Malaysian Agricultural research and Development Institute (MArDI). The current study used next generation sequencing (NGS) technologies to develop starfruit simple sequence repeat (SSr) from 2 varieties namely B11 and B17 using Illumina HiSeq. The pre-processed reads were de novo assembled to generate approximately 75,000 and 74,000 scaffolds respectively. Total genome size for B11 and B17 were around 345 Mbp and 342 Mbp based on K-mer distribution analysis. In-silico microsatellite mining of each variety has identified more than 17,000 SSr in B11 and B17 respectively. Dinucleotides were the most abundant, accounting for more than 70% of all SSr and repeat motif GA (49%) was most common. A total of 239 SSr primer pairs were designed from contigs larger than 350 nucleotides and tested for amplification. The 30 polymorphic SSrs were used to DNA fingerprint of 12 starfruit hybrids. Polymorphism information content (PIC) ranged from 0.1411 to 0.6838, with an average of 0.3919. The Unweighted Pair-Group Method for Arithmetic Averages (UPGMA) dendrogram clustered 12 starfruit accessions into 2 groups.

关键词： Starfruit (Averrhoa carambola L.) next generation sequencing molecular marker simple sequence repeat

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relationship between peripheral arterial disease, carotid intima-media thickness and C-reactive protein in elderly diabetic patients

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Advances in Aging research 2013年第4期2卷 115-120页

作者： Moatassem Salah Amer Samia Ahmed abdul-rahman Wafaa Mostafa Abd-El Gawad Ibrahim Ahmad Abdel Aal Ahmad Abdel Khalek Abdel razek Mohamed Wessam El-Huseiny Moustafa Abdel Wahab Clinical Pathology Department Faculty of Medicine Mansoura University Mansoura Egypt Geriatrics and Gerontology Department Faculty of Medicine Ain Shams University Cairo Egypt Geriatrics and Gerontology Department Faculty of Medicine Mansoura University Mansoura Egypt radiodiagnosis Department Faculty of Medicine Mansoura University Mansoura Egypt

Purpose: To study the association between carotid intima media thickness (CIMT) and high sensitivity CrP (hs-CrP) level as markers for PAD in elderly patients with DM. Subjects: A case-control study on 90 participants aged 60 years and older divided into 60 cases (30 patients with DM alone and 30 patients with DM and comorbidities) and 30 healthy controls. All were assessed by measuring CIMT, ankle brachial index (AB), and markers for cardiovascular disease such as high-sensitivity CrP (hs-CrP), total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL), and low density lipoprotein (LDL). results: hs-CrP levels showed statistically significant difference being highest among patients with DM and comorbidities and lowest among controls (P re significantly higher among cases than controls. ABI was able to detect PAD in many asymptomatic patients. Color changes were present in only 43.30% (n = 26) of positive PAD cases while delayed wound healing, claudication pain, rest pain, cold extremities, and trophic changes were present in 23.30% (n = 14), 16.71% (n = 10), 16.71% (n = 10), 45.00% (n = 27), and 21.7% (n = 13). Using logistic regression analysis revealed that DM, CIMT, and hs-CrP were independent predictors for PAD (Or = 4.194, 7.236, 1.003;P value = 0.044, 0.25, 0.031) after adjustment of other coronary risk factors such as sex, smoking, hypertension, TC, and TG. Conclusion: Diabetic elderly have higher prevalence of asymptomatic PAD thannon-diabetics using solely ABI. DM, CIMT, and hs-CrP are independent predictors for the occurrence of PAD. Hs-CrP levels are highest among diabetics with comorbidities.

关键词： Peripheral Arterial Disease Carotid Intima-Media Thickness Ankle Brachial Index C-reactive Protein Elderly

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质量建设中的安全管理

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工程造价管理 2006年第6期 47-51页

作者： Husrul Nizam Husin Syed abdul Haris Mustapa Natasha Khalil 朱兰波（翻译）马拉技术大学（University Technology MArA）马来西亚霹雳州东北财经大学研究生部

通过对马来西亚建筑项目的个体研究显示出其建筑设备机械的安全管理现状以及有可能引发的问题，本文旨在基于此研究结粜，强调建筑设备机械的安全管理的必要性。在过去几年中，政府致力于通过贯彻指导方针来实施安全健康政策，开展现场... 详细信息

通过对马来西亚建筑项目的个体研究显示出其建筑设备机械的安全管理现状以及有可能引发的问题，本文旨在基于此研究结粜，强调建筑设备机械的安全管理的必要性。在过去几年中，政府致力于通过贯彻指导方针来实施安全健康政策，开展现场安全研讨会以及实施项目现场安全认证制度。建筑业作为马来西亚的第二大工业，同时又是工作现场事故发生最多的行业，由于不安全的施工现场引发的事故以及因此损失的利润使其深受影响。统计数据表明建筑设备及机械是造成这些事故的原因之一，因此，本文以“质量建设中的安全管理”为题，意在说明对于建筑设备和机械的安全管理的关系和重要性。同时设计一个可以用来作为管理指导方针的实用模型。“安全管理”对于马来西亚来说是一个新的管理概念，本文指出在马来西亚实施安全管理的范围，同时提出对于建筑设备机械进行安全管理的合理框架，通过研究发现，安全管理对于马来西亚而言仍是一个很新的概念，职业安全与健康法案，尤其是针对于建筑设备机械使用的安全与健康法案在马来西亚仍是空缺。另外，研究表明．在项目现场设立安全委员会是实旅安全管理的主要措施，但除此以外，安全管理的其他方面还未能被清楚阐明。建筑设备机械的安全管理需要一个长时间及过程的调整。这将使其不可能在短时间内得以实施。因此，本文吸取了研究中的发现，利用指示方法建立了一个用以详细说明建筑设备机械安全管理这一基本概念的实用框架模型。模型采用Walker.A（1993）提出的管理实用基础模型作为原型用以突出其关键元素，而其关键节点或关键过程则是基于个体调查得出的结果。

关键词：建筑业安全质量管理建设业质量关系实施模型输入转换过程输出加强

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Probiotic enhanced intestinal immunity in broilers against subclinical necrotic enteritis

Probiotic enhanced intestinal immunity in broilers against s...

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中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Jinge Xin Hesong Wang Xueqin Ni Xiaodan Qing Lei Liu Jing Lai abdul Khalique Guangyao Li Kangcheng Pan Bo Jing Dong Zeng Animal Microecology Institute College of VeterinarySichuan Agricultural University

Along with banning of antibiotics,necrotic enteritis(NE),especially subclinical NE(SNE) whereby no clinical signs are present in chicks,has become one of the most threatening problems in poultry ***,increasing attention has been focused on research and application of effective probiotic strains,as an alternative to antibiotics,to prevent SNE in *** the present study,we evaluated the effects of Lactobacillus johnsonii BS15 on prevention of SNE in ***,assessment determined growth performance and indexes related to intestinal mucosal immunity in the ileum and cecal tonsil of broilers.A total of 300 one-day-old Cobb 500 chicks were randomly distributed into the following five groups:control group(fed with basal diet + de Man,rogosa,and Sharpe liquid medium[normal diet]),SNE group(normal diet),BS15 group(basal diet + 1 × 10 colony-forming units BS15/g as fed[BS15 diet]),treatment group(normal diet[days 1-28]+ BS15 diet[days 29-42]),and prevention group(BS15 diet[days 1-28]+ normal diet[days 29-42]) throughout a 42-day experimental *** infection was treated for all chicks in the SNE,BS15,treatment,and prevention *** present results demonstrated that BS15 supplementation of feeds in BS15 and prevention groups exerted a positive effect on preventing negative influences on growth performance;these negative influences included low body weight gain and increased feed conversion ratio caused by *** no changes were detected in all determined indexes in cecal tonsils,BS15-treated broilers were free from SNE-caused damage in villi in the ***15 inhibited SNE-caused decrease in immunoglobulins in the *** the lamina propria of ileum,T-cell subsets of lymphocytes influenced by SNE were also controlled by ***15 affected antioxidant abilities of the ileum and controlled SNE-induced mitochondrion-mediated apoptosis by positively changing contents and/or mrNA expression levels of apoptosis-related ***

关键词： Subclinical necrotic enteritis Lactobacillus johnsonii intestinal immunity probiotic broiler

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An Investigation into Infection Prevention and Control Practices among Close Contacts of COVID-19 Positive Cases Identified during Trace Test and Quarantine Activities at District Quetta (Unmatched Case-Control Study)

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Open Journal of Epidemiology 2021年第4期11卷 360-370页

作者： Muhammad Arif Abid Saeed Muhammad abdullah Ambreen Chaudhary Zakir Hussain Mirza Zeeshan Iqbal Baig Zubair Ahmed Khoso Mir abdul Qadir Sheikh Ahmed Saher Sultan Zahra Gauhar Ayesha Babar Kawish Ehsan Larik Department of Health FELTP Pakistan Quetta Pakistan Department of Health WHO Quetta Pakistan Department of Health FELTP Pakistan Islamabad Pakistan Department of Health IPH Quetta Quetta Pakistan Department of Community & Preventive Dentistry AMC NUMS Rawalpindi Pakistan Department of Health BMC Quetta Pakistan Department of Health PIO Rawalpindi Pakistan

The second wave of COVID-19 pandemic has started globally, right now 220 countries are infected and a total of 71,351,695 confirmed cases and 1,612,372 deaths due to COVID-19 have been reported. Infection Prevention and Control (IPC) measures for COVID-19 all have proved vital in decreasing the transmission rates among the communities. rong>Methodology:rong> Unmatched Case-Control Study was conducted where cases were defined as “every PCr positive contact (symptomatic or asymptomatic) for any index case” similarly controls were defined as “every PCr negative contact (symptomatic or asymptomatic) for any index case who was home quarantined for 14 days based on suspicion by PDSrU team”. A simple random technique was used and 300 individuals were made part of this study. rong>results:rong> The major findings of this study shows that PCr positive contacts poorly adopted certain COVID-19 IPC measures of interest in their daily life hence got infected. The odds for all the variables of interest were found to be statistically significant among cases as compared to controls like the odds for knowingly and intentionally contacted with a COVID-19 positive case was 13.7 times more among the PCr positive contacts as compare to PCr negative contacts (p = 0.00, C.I = 7.62 - 24.90), similarly, the odds of being a family member of the index COVID-19 case was 7.07 times more among the PCr positive contacts as compared to the PCr negative contacts (p = 0.00, C.I = 3.25 - 15.86). rong>Conclusion:rong> Before the development and availability of a vaccine, the only tools that can help prevent the spread of COVID-19 are IPC measures.

关键词： COVID-19 IPC Measures Contact PCr PDSrU

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采用有限元法通过改进结构消除鼓式制动器噪声

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传动技术 2012年第4期26卷 32-41页

作者： Abd rahim Abu—Bakar Mohd reaza Buang roslan abdul rahman Mohd Zam Abu rashid Universiti Teknologi Malaysia Universiti Teknologi Malaysia Johor. Universiti Teknologi Malaysia.

降低制动器噪声已成为制动器工程师们具有挑战性的任务。目前关于处理盘式制动器噪声方面已有许多建议,但过去在鼓式制动器系统方面解决方法很少。本文企图提出一种采用有限元方法通过改进结构,降低鼓式制动器噪声。开发并证实了一个真... 详细信息

降低制动器噪声已成为制动器工程师们具有挑战性的任务。目前关于处理盘式制动器噪声方面已有许多建议,但过去在鼓式制动器系统方面解决方法很少。本文企图提出一种采用有限元方法通过改进结构,降低鼓式制动器噪声。开发并证实了一个真实鼓式制动器的三维有限元(FE)模型。首先用模拟的基本有限元模型预测噪声,然后再提出几个改进结构,最后对选择的改进模型进行动态不稳定过程分析,以便确认由复杂的固有值分析得出的预测结果。

关键词：鼓式制动器有限元结构改进噪声复杂固有值不稳定过程分析

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Pain Efficacy of a Home-Based Low-Intensity Continuous Ultrasound Stimulator for Knee Arthritis: A Single-Arm, Open-Label, Prospective Clinical Trial

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Health 2024年第7期16卷 703-716页

作者： Md. Habibur rahman Yeon-Gyu Jang Johny Bajgai Subham Sharma Kchorng Vira abdul-Nasir Sofian Seong Hoon Goh Yundeok Kim Cheol-Su Kim Doo-Sup Kim Kyu-Jae Lee Department of Convergence Medicine Wonju College of Medicine Yonsei University Wonju Republic of Korea Department of Neurosurgery Wonju Severance Christian Hospital Wonju College of Medicine Yonsei University Wonju Republic of Korea Department of Global Medical Science Wonju College of Medicine Yonsei University Wonju Republic of Korea Department of Internal Medicine Division of Hematology-Oncology Wonju Severance Christian Hospital Wonju Republic of Korea Department of Orthopaedic Surgery Wonju College of Medicine Yonsei University Wonju Republic of Korea

1) Background: Osteoarthritis (OA) is defined as a degenerative joint disease that mainly affects the bone. This study aims to evaluate the effect of low-intensity continuous ultrasound (LICUS) treatment on the knee of osteoarthritis patients through home-based intervention using the LICUS medical device. 2) Methods: The clinical trials were designed in a single-arm, open-label, and intervention study. Thirty-five participants, including those who dropped out (12%), were screened and enrolled. The patients received LICUS (1.1 MHz, 1.5 W/cm2, collimated beams) on the knee by the instructions of the investigator at home (5 min/session, 3 times/day, for four-weeks). Outcome measures were assessed using the Visual Analog Scale (VAS) as a primary endpoint and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) as a secondary endpoint to evaluate pain relief and functional recovery of the knee between pre-treatment (baseline) and post-treatment (four-weeks). 3) results: Knee pain scores measured using the VAS and WOMAC indices were significantly reduced after a four-week treatment with LICUS compared to baseline. Knee stiffness and functional capacity were significantly reduced after the LICUS application. In addition, there were no reports of adverse effects during the study period. 4) Conclusion: Long-term and home-based application of LICUS can be recommended as an alternative option for the treatment of OA patients, as evidenced by the effect of pain relief and knee function recovery.

关键词： Knee Osteoarthritis Low-Intensity Continuous Ultrasound Stimulator Clinical Trial Visual Analogue Scale Western Ontario and McMaster Universities Osteoarthritis Index

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CPPU、GA3处理对藤稔葡萄生物学性状及其生理基础效应的研究

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中国农业科学 1998年第1期31卷 93-95页

作者：哈基姆张上隆陈大明陈履荣陈昆松徐昌杰陈志辉浙江农业大学园艺系

ＣＰＰＵ、ＧＡ３处理对藤稔葡萄生物学性状及其生理基础效应的研究哈基姆＊张上隆陈大明陈履荣陈昆松徐昌杰陈志辉（浙江农业大学园艺系，杭州３１００２９）ＥｆｅｃｔｓｏｆＣＰＰＵ，ＧＡ３ＴｒｅａｔｍｅｎｔｓｏｎＦｒｕｉｔＤｅ... 详细信息

ＣＰＰＵ、ＧＡ３处理对藤稔葡萄生物学性状及其生理基础效应的研究哈基姆＊张上隆陈大明陈履荣陈昆松徐昌杰陈志辉（浙江农业大学园艺系，杭州３１００２９）ＥｆｅｃｔｓｏｆＣＰＰＵ，ＧＡ３ＴｒｅａｔｍｅｎｔｓｏｎＦｒｕｉｔＤｅｖｅｌｏｐｍｅｎｔｏｆＦｕｊｉｍｉ...

关键词：藤稔葡萄 CPPU GA3 果实生长发育

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中国对阿拉伯世界人文外交的历史回顾及现实挑战

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阿拉伯世界研究 2011年第6期 53-59页

作者：闵捷马云蔚上海外国语大学中东研究所沙特-中国商务理事会

人文外交作为一种崭新的外交形式,是对当今国际关系领域中国家、市场与社会三者互动的有效应对,也是对文化外交和公共外交的有机整合。人文外交以软实力理论、国家形象理论、建构主义理论和文明对话理论为依托,在当今中国的外交政策中... 详细信息

人文外交作为一种崭新的外交形式,是对当今国际关系领域中国家、市场与社会三者互动的有效应对,也是对文化外交和公共外交的有机整合。人文外交以软实力理论、国家形象理论、建构主义理论和文明对话理论为依托,在当今中国的外交政策中占有重要地位。新中国对阿拉伯世界的外交实践则为人文外交的理论创新积累了宝贵经验。在对阿关系中,人文外交在各时期都促进了中国国家利益的拓展。当下,国际体系处于转型期,尤其是2010年年底以来阿拉伯世界所发生的社会和政治动荡,使中国对阿拉伯世界的外交政策面临挑战。只有将人文外交继续作为我国对阿外交政策的重点,才能在政治动荡的阿拉伯世界始终维护中国的国家利益。

关键词：中国对外政策阿拉伯世界人文外交国家利益公共外交

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Serological Investigation of Laboratory-Confirmed and Suspected Ebola Virus Disease Patients During the Late Phase of the Ebola Outbreak in Sierra Leone

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Virologica Sinica 2018年第4期33卷 323-334页

作者： Yang Liu YuLan Sun Wei Wu AQian Li XianDa Yang Shuo Zhang Chuan Li QiuDong Su ShaoJian Cai DaPeng Sun HaiYang Hu Zhe Zhang XiuXu Yang Idrissa Kamara Sheku Koroma Gerald Bangura Alie Tia abdul Kamara Matt Lebby Brima Kargbo Jiandong Li Shiwen Wang XiaoPing Dong YueLong Shu WenBo Xu George F. Gao GuiZhen Wu DeXin Li William J. Liu MiFang Liang Key Laboratory of Medical Virology and Viral Diseases Ministry of Health National Institute for Viral Disease Control and Prevention (IVDC) Chinese Center for Disease Control and Prevention (China CDC) Beijing 102206 China Beijing Center for Disease Control and Prevention (Beijing CDC China) Beijing 100013 China Jilin Provincial Center for Disease Control and Prevention (Jilin CDC China) Jilin 130021 China Fujian Provincial Center for Disease Control and Prevention (Fujian CDC China) Fuzhou 350001 China Shandong Provincial Center for Disease Control and Prevention (Shandong CDC China) Jinan 250014 China Jiangsu Provincial Center for Disease Control and Prevention (Jiangsu CDC China) Nanjing 210009 China Beijing Institute of Biotechnology Beijing 100071 China Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Bio-safety Lab) Freetown 999127 Sierra Leone The Ministry of Health and Sanitation (MoHS SL) Freetown 999127 Sierra Leone CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China

This study aimed to investigate the serological characteristics of Ebola virus(EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease(EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction(rT-PCr) for viral rNA and enzyme-linked immunosorbent assay(ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015. Viral rNA load decreased rapidly at earlier stages after disease onset, while EBOV-specific IgM and IgG still persisted in 58.1%(18/31) and 93.5%(29/31) of the confirmed EVD patients and in 3.8%(25/663) and 17.8%(118/663) of the rNA-negative suspected patients in the later phase, respectively. Dynamic analysis of longitudinally collected samples from eight EVD patients revealed typically reversed trends of declining viral load and increasing IgM and/or IgG titers in response to the EBOV *** present results indicate that certain populations of Sierra Leone developed immunity to an EBOV infection in the late phase of the outbreak, providing novel insights into the risk assessment of EBOV infections among human populations.

关键词： Ebola virus (EBOV) Late phase Serologic investigation IgM IgG

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