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Determination of Fatty Acid Composition by GC-MS and Potential Antioxidant and Antibacterial Properties of rumex hastatus and Cytisus scoparius Seeds Grown in Kashmir

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Journal of Pharmacy and Pharmacology 2021年第2期9卷 64-78页

作者： Zubair rehman Nengroo abdul rauf Shayeeb Ahmad Bhat Muzammil Sharief Dar Department of Chemistry Aligarh Muslim UniversityAligarh 202002India Department of Post Harvest Engineering and Technology Aligarh Muslim UniversityAligarh 202002India Department of Microbiology Aligarh Muslim UniversityAligarh 202002Uttar PradeshIndia

rumex hastatus D. Don and Cytisus scoparius Linn. are medicinally important plants used in Ayurveda and food, areenriched with remarkable nervine, diuretic, sedative and various other therapeutic properties. In this study Soxhlet extractionproduces higher yield of seed extracts. Gas chromatography-mass spectrometry was used to determine fatty acid composition ofpetroleum ether extracts (PE). The major fatty acids were Linolenic acid 53.53% in r. hastatus and linoleic acid 59.42% in ***. Palmitic acid was also found in significant amount (16.58% and 19.33%) in r. hastatus and C. scoparius respectively. Thechloroform (rCF, CCF), ethyl acetate (rEA, CEA), acetone (rAT, CAT) and methanol (rMT, CMT) extracts of r. hastaus and *** respectively, were examined for their antioxidant activity using 2,2-di-phenyl-1-picrylhydrazyl (DPPH), nitrobluetetrazolium (NBT), hydrogen peroxide (H2O2), 2,2ˈ-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavengingassay and antibacterial activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus by Agar disc diffusionmethod. All the extracts showed good radical scavenging and antibacterial activities compared to butylated hydroxyltoluene andstreptomycin respectively. However, dominant radical scavenging effect was observed at 100 μg/mL and 200 μg/mL concentrationsof extracts and dominant zone of inhibition was shown by CAT, CMT, rAT and rEA extracts. This study exhibited beneficialproperties of these plants as an ingredient (unsaturated fatty acids) for the formulation of various functional foods and a source ofnatural antioxidant and antibacterial agents for drug, design and development.

关键词： Soxhlet extraction Linoleic acid Linolenic acid Antibacterial activity Antioxidant activity

Unsteady thin film flow of a fourth grade fluid over a vertical moving and oscillating belt

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Propulsion and Power research 2016年第3期5卷 223-235页

作者： Taza Gul Fazle Ghani S.Islam r.A.Shah I.Khan Saleem Nasir S.Sharidand Department of Mathematics Abdul Wali Khan UniversityMardanKPKPakistan Department of Mathematics University Engineering Technology PeshawarKPKPakistan Department of Basic Sciences College of Engineering Majmaah UniversityP.O.Box 66Majmaah 11952Saudi Arabia Department of Mathematical Sciences Faculty of ScienceUniversiti Teknologi MalaysiaUTM Johor Bahru81310 JohorMalaysia

This article studies the unsteady thin film flow of a fourth grade fluid over a moving and oscillating vertical *** problem is modeled in terms of non-nonlinear partial differential equations with some physical *** problems of lift and drainage are *** different techniques namely the adomian decomposition method(ADM)and the optimal homotopy asymptotic method(OHAM)are used for finding the analytical *** solutions are compared and found in excellent *** the physical analysis of the problem,graphical results are provided and discussed for various embedded flow parameters.

关键词： Unsteady flow Thin film Lifting and drainage Fourth grade fluid Adomian decomposition method(ADM) Optimal homotopy asymptotic method(OHAM)

Cassava Groundnut Intercropping: A Sustainable Land Management Practice for Increasing Crop Productivity and Organic Carbon Stock on Smallholder Farms

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Prevalence of epilepsy in the onchocerciasis endemic middle belt of Ghana after 27 years of mass drug administration with ivermectin

Agricultural Sciences（农业科学） 2023年第1期

作者： Keiwoma M. Yila Mohamed S. Lebbie abdul r. Conteh Mohamed S. Kamara Lamin I. Kamara Mathew L. S. Gboku

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Infectious Diseases of Poverty 2023年第4期12卷 106-106页

作者： Kenneth Bentum Otabil Blessing Ankrah Emmanuel John Bart-Plange Emmanuel Sam Donkoh Fiona Amoabil Avarikame Fredrick Obeng Ofori-Appiah Theophilus Nti Babae Prince-Charles Kudzordzi Vera Achiaa Darko Joseph Ameyaw Joseph Gyekye Bamfo raji abdul Sakibu Daniel Antwi-Berko Joseph Nelson Siewe Fodjo María-Gloria Basáñez Henk D.F.H.Schallig robert Colebunders NeTroDis research Group Centre for Research in Applied Biology School of Sciences University of Energy and Natural Resources Sunyani Ghana Department of Biological Science School of Sciences University of Energy and Natural Resources Sunyani Ghana Global Health Institute University of Antwerp Antwerp Belgium Department of Medical Laboratory Science School of Sciences University of Energy and Natural Resources Sunyani Ghana STU Clinic Sunyani Technical University Sunyani Ghana Happy Family Hospital Nkoranza Ghana Tain District Hospital Nsawkaw Ghana Department of Infectious Disease Epidemiology MRC Centre for Global Infectious Disease Analysis (MRC GIDA) and London Centre for Neglected Tropical Disease Research School of Public Health Imperial College London London UK Department of Medical Microbiology Experimental Parasitology Unit Amsterdam University Medical Centres Academic Medical Centre at the University of Amsterdam Amsterdam The Netherlands

BackgroundIn onchocerciasis-endemic areas with high ongoing Onchocerca volvulus transmission,a high prevalence of epilepsy has been *** study aimed to determine the prevalence and clinical characteristics of epilepsy in the Bono region of Ghana following 27 years of implementation of ivermectin mass drug administration(MDA).MethodsBetween October 2020 and August 2021,cross-sectional surveys were conducted in nine communities in the Tain District and Wenchi Municipality of the Bono region of *** the first stage,a random door-to-door approach was used to screen the population for epilepsy using a pre-tested *** suspected of having epilepsy were invited for a second-stage neurological examination for case *** *** microfilarial infection status and Ov16 seropositivity were also ***-five confidence intervals(95%CI)for prevalence values were calculated using the Wilson Score *** the 971 participants,500(51.5%)were females,and the median age(interquartile range)was 26(15‒43)*** participants(1.4%,95%CI:1.0‒2.0)were diagnosed as having epilepsy with generalized seizures being the most frequent seizure type(85.7%,12/14).The overall microfilarial prevalence of *** was 10.3%(November 2020)and 9.9%(August 2021);the Ov16 seroprevalence was 22.2%(June 2021).Only 63.2%took ivermectin in the last round of MDA distribution in March *** 1.4%prevalence of epilepsy in the Bono region is similar to the median epilepsy prevalence in sub-Saharan ***,the persistent microfilarial prevalence and low ivermectin study coverage call for the Ghana Onchocerciasis Elimination Programme to step up its efforts to ensure that the gains achieved are consolidated and improved to achieve the elimination of onchocerciasis by 2030.

关键词： Onchocerca volvulus Microflaria Onchocerciasis-associated epilepsy Seizure Ivermectin Mass drug administration Coverage

Modeling of Sensor Enabled IrrigationManagement for Intelligent Agriculture Using Hybrid Deep Belief Network

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Computer Systems Science & Engineering 2023年第8期46卷 2319-2335页

作者： Saud Yonbawi Sultan Alahmari B.r.S.S.raju Chukka Hari Govinda rao Mohamad Khairi Ishak Hend Khalid Alkahtani JoséVarela-Aldás Samih M.Mostafa Department of Software Engineering College of Computer Science and EngineeringUniversity of JeddahJeddahSaudi Arabia King abdul Aziz City for Science and Technology RiyadhKingdom of Saudi Arabia Aditya Engineering College Affiliated to JNTUK(Kakinada) SurampalemIndia Department of MBA Vignan’s Institute of Information and Technology(A)DuvvadaVisakhapatnamAP530049India School of Electrical and Electronic Engineering Engineering CampusUniversiti Sains Malaysia(USM)Nibong TebalPenang14300Malaysia Department of Information Systems College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityRiyadh11564Saudi Arabia SISAu research Group Universidad Tecnológica IndoaméricaAmbato180103Ecuador Faculty of Computers and Information South Valley UniversityQena83523Egypt

Artificial intelligence(AI)technologies and sensors have recently received significant interest in intellectual *** the application of AI technologies and agriculture sensors in intellectual agriculture is urgently required for the growth of modern agriculture and will help promote smart *** irrigation scheduling systems were highly required in the agricultural field due to their capability to manage and save water deficit irrigation *** learning systems devise an alternative to conventional irrigation management through the automatic elaboration of predictions related to the learning of an *** this motivation,this study develops a modified black widow optimization with a deep belief network-based smart irrigation system(MBWODBN-SIS)for intelligent *** MBWODBN-SIS algorithm primarily enables the Internet of Things(IoT)based sensors to collect data forwarded to the cloud server for examination ***,the MBWODBN-SIS technique applies the deep belief network(DBN)model for different types of irrigation classification:average,high needed,highly not needed,and not *** MBWO algorithm is used for the hyperparameter tuning process.A wideranging experiment was conducted,and the comparison study stated the enhanced outcomes of the MBWODBN-SIS approach to other DL models with maximum accuracy of 95.73%.

关键词： Agriculture smart farming hyperparameter tuning artificial intelligence irrigation management sensors deep learning

实时剪切波弹性成像在肾小球肾炎早期诊断中的应用价值

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医学信息 2020年第9期33卷 90-92页

作者：阿丽米热·阿不都热依木宋涛新疆医科大学第一附属医院腹部超声诊断科

目的初步探讨实时剪切波弹性成像（SWE）对于成人早期慢性肾小球肾炎（CGN）的肾脏纤维化的诊断价值。方法选取我院2018年1～12月经病理确诊的早期CGN患者40例,根据我国改良简化的MDrD方程估测的肾小球滤过率（GFr）分为CGN1期组20例[GF... 详细信息

目的初步探讨实时剪切波弹性成像（SWE）对于成人早期慢性肾小球肾炎（CGN）的肾脏纤维化的诊断价值。方法选取我院2018年1～12月经病理确诊的早期CGN患者40例,根据我国改良简化的MDrD方程估测的肾小球滤过率（GFr）分为CGN1期组20例[GFr≥90 ml/（min·1.73 m2）],CGN2期组20例[GFr 60～89 ml/（min·1.73 m2）],另选取同期健康体检人群30名设为健康对照组,比较两组肾脏杨氏模量、肾脏长径、宽径及肾下极实质厚度。采用Pearson方法对于肾脏杨氏模量与生化指标进行相关性分析,以病理结果为标准,建立rOC曲线确定SWE评价早期CGN的诊断临界点。结果 CGN组与健康对照组肾脏长径、宽径及肾下极实质厚度比较,差异均无统计学意义（Pr呈负相关（r=-0.591、-0.588、-0.579,PrOC曲线下面积为0.781。结论剪切波弹性成像技术可定量评价肾脏组织弹性,反映肾功能的改变,可用于CGN的早期诊断。

关键词：实时剪切波弹性成像纤维化慢性肾小球肾炎杨氏模量

In vitro inhibitory analysis of consensus sirNAs against NS3 gene of hepatitis C virus 1a genotype

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 763-770页

作者： Imran Shahid Waleed Hassan Al Malki Mohammed Wanees Al rabia Mohammed Hasan Mukhtar Shaia Saleh r.Almalki Saad Ahmed Alkahtani Sami S.Ashgar Hani S.Faidah Muhammad Hassan Hafeez Department of Pharmacology and Toxicology College of PharmacyUmm Al Qura University Department of Medical Microbiology College of MedicineKing Abdul Aziz University Department of Biochemistry Faculty of MedicineUmm Al Qura University Department of Laboratory Medicine Faculty of Applied Medical SciencesAlbaha University College of Pharmacy Najran University Department of Microbiology College of MedicineUmm Al Qura University Department of Gastroenterology and Hepatology Fatima Memorial College of Medicine and Dentistry

Objective: To explore inhibitory effects of genome-specific, chemically synthesized sirNAs(small interference rNA) against NS3 gene of hepatitis C virus(HCV) 1a genotype in stable Huh-7(human hepatoma) cells as well as against viral replication in serum-inoculated Huh-7 cells. Methods: Stable Huh-7 cells persistently expressing NS3 gene were produced under antibiotic gentamycin(G418) selection. The cell clones resistant to 1 000 μg antibiotic concentration(G418) were picked as stable cell clones. The NS3 gene expression in stable cell clone was confirmed by rT-PCr and Western blotting. sirNA cell cytotoxicity was determined by MTT cell proliferation assay. Stable cell lines were transfected with sequence specific sirNAs and their inhibitory effects were determined by rT-PCr, real-time PCr and Western blotting. The viral replication inhibition by sirNAs in serum inoculated Huh-7 cells was determined by real-time PCr. results: rT-PCr and Western blot analysis confirmed NS3 gene and protein expression in stable cell lines on day 10, 20 and 30 post transfection. MTT cell proliferation assay revealed that at most concentrated dose tested(50 nmol/L), sirNA had no cytotoxic effects on Huh-7 cells and cell proliferation remained unaffected. As demonstrated by the sirNA time-dependent inhibitory analysis, sirNA NS3-is44 showed maximum inhibition of NS3 gene in stable Huh-7 cell clones at 24(80%, P=0.013) and 48 h(75%, P=0.002) post transfection. The impact of sirNAs on virus replication in serum inoculated Huh-7 cells also demonstrated significant decrease in viral copy number, where sirNA NS3-is44 exhibited 70%(Pral rNA reduction as compared to NS3-is33, which showed a 64%(Prease in viral copy number. sirNA synergism(NS3-is33 + NS3-is44) decreased viral load by 84%(Pred to individual inhibition by each sirNA(i.e., 64%–70%(Prum-inoculated cells. Synthetic sirNAs mixture(NS5Bis88 + NS3-is33) targeting different region of HCV genom

关键词： Hepatitis C virus NS3 protein Stable Huh-7 cell culture system rNA interference NS5B HCV therapeutics

响应面法优化红景天多酚提取工艺及其体外α-葡萄糖苷酶抑制活性

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食品工业科技 2022年第6期43卷 213-220页

作者：任卫合罗龙龙王丽萍高彩雯余金凤皮尔·穆罕默德·阿卜杜勒丁功涛陈士恩西北民族大学生命科学与工程学院甘肃兰州730124 西北民族大学生物研究中心中国-马来西亚国家联合实验室甘肃兰州730124 马来西亚国立大学工程与建筑环境学院雪兰莪邦吉43600

该研究首先采用单因素及Box-Behnken响应面试验优化红景天多酚提取工艺,然后构建体外α-葡萄糖苷酶抑制体系,研究红景天多酚α-葡萄糖苷酶抑制活性,同时通过酶抑制动力学,判断其抑制类型。单因素及响应面结果表明,最佳提取工艺条件为:... 详细信息

该研究首先采用单因素及Box-Behnken响应面试验优化红景天多酚提取工艺,然后构建体外α-葡萄糖苷酶抑制体系,研究红景天多酚α-葡萄糖苷酶抑制活性,同时通过酶抑制动力学,判断其抑制类型。单因素及响应面结果表明,最佳提取工艺条件为:乙醇浓度71%、料液比1:40、超声功率320 W、超声温度55℃,此条件下红景天多酚提取得率可达11.45%;红景天多酚对α-葡萄糖苷酶具有一定的抑制活性,且抑制能力呈量效关系,半数抑制浓度(IC_(50))为2.83 mg/mL,低于阳性对照阿卡波糖(IC_(50)为3.36 mg/mL),当红景天多酚浓度为50 mg/mL时,抑制率可达97.86%;酶抑制动力学研究表明,红景天多酚对α-葡萄糖苷酶为可逆混合型抑制类型,随着抑制剂浓度增大,其最大反应速率Vmax减小、米氏常数Km增大,属于竞争与非竞争性混合类型。此优化试验有效可行,且提取的红景天多酚在体外对α-葡萄糖苷酶具有较好抑制活性。

关键词：红景天多酚响应面 α-葡萄糖苷酶酶抑制动力学

Physico-chemical parameters for the assembly of moxifloxacin hydrochloride and cetyltrimethylammonium chloride mixture in aqueous and alcoholic media

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