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Sodium nitroprusside as a signal molecule for up-regulating membrane characteristics,antioxidant defense system to improve flax productivity under water stress

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Oil Crop Science 2024年第3期9卷 160-169页

作者： N.M.Al-Ashkar B.A.Bakry H.M.S.El-Bassiouny M.M.S.Abdallah M.S.sadak Botany Department Agricultural and Biological Research InstituteNational Research Centre33 El Bohouth Street P.O.12622DokkiGizaEgypt Field Crops Research Department Agricultural and Biological Research InstituteNational Research Centre33 El Bohouth Street P.O.12622DokkiGizaEgypt

Water stress is a critical environmental adversity that significantly impacts the growth,development,and yield of flax *** this study,flax seeds were cultivated under different water irrigation requirements(WIR)(100%,75%,and 50%)to investigate the effects of exogenously supplied nitric oxide(NO)donor sodium nitroprusside(SNP)as foliar treatments at concentrations of 0.0 mmol/L,0.5 mmol/L,1.0 mmol/L,and 2.0 mmol/*** stress led to a significant decrease in plant growth,photosynthetic pigments,yield components such as oil and total carbohydrate *** also resulted in an increase in leaf H2O2 production,lipid peroxidation levels and activities of enzymatic antioxidants including polyphenol oxidase,superoxide dismutase,and nitrate reductase ***,foliar application of SNP improved photosynthetic pigments and antioxidant defense system which mitigated the negative impact of water stress on growth and yield productivity by reducing oxidative damage caused by reactive oxygen species *** use of SNP also decreased H_(2)O_(2) accumulation levels,lipid peroxidation levels,and improved membrane *** treatment at concentration of 2 mmol/L showed superior results compared to other concentrations with extremely significant increases observed in yield characteristics such as oil content,total carbohydrate percentages,and unsaturated fatty acids to saturated fatty acids ratio.

关键词： Flax Fatty acid Sodium nitroprusside Antioxidant enzymes Yield

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Enhancing Canola Yield and Photosynthesis under Water Stress with Hydrogel Polymers

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1623-1645页

作者： Elham A.Badr Gehan Sh.Bakhoum Mervat Sh.sadak Ibrahim Al-Ashkar Mohammad Sohidul Islam Ayman El Sabagh Magdi T.Abdelhamid Field Crops Research Department National Research CentreCairo12622Egypt Botany Department National Research CentreCairo12622Egypt Plant Production Department College of Food and Agriculture SciencesKing Saud UniversityRiyadh12271Saudi Arabia Department of Agronomy Hajee Mohammad Danesh Science and Technology UniversityDinajpur5200Bangladesh Department of Field Crops Faculty of AgricultureSiirt UniversitySiirt56100Turkey Department of Agronomy Faculty of AgricultureKafrelsheikh UniversityKafrelsheikh33516Egypt Department of Research Centers Montana State UniversityBozemanMT 59717USA

While Egypt’s canola production per unit area has recently grown,productivity remains low,necessitating increased *** are water-absorbent polymer compounds that can optimize irrigation schedules by increasing the soil’s ability to retain ***,twoﬁeld experiments were conducted to examine hydrogel application to sandy soil on canola growth,biochemical aspects,yield,yield traits,and nutritional quality of yielded seeds grown under water deﬁcit stress *** experiments were conducted by arranging a split-plot layout in a randomized complete block design(RCBD)with three times replications of each *** water stress at 75%or 50%of crop evapotranspiration(ETc)lowered chlorophyll a,chlorophyll b,caro-tenoids,and total pigments content,indole-3-acetic acid,plant development,seed yield,and oil and total carbo-hydrates of seed yield,hydrogel treatment enhanced all of the traits mentioned ***,hydrogel enhanced to gather compatible solutes(proline,amino acids,total soluble sugars),phenolics content in leaves,seed protein,and crop water productivity,which increased while the plants were under water *** results revealed that the full irrigation(100%ETc)along with hydrogel compared to water-stressed(50%ETc)led to enhanced seed yield(kg ha^(-1)),Oil(%),and Total carbohydrates(%)of rapeseed by 57.1%,11.1%and 15.7%,***,under water-stressed plots with hydrogel exhibited enhancement by 10.0%,3.2%and 5.1%in seed yield(kg ha^(-1)),oil(%),and total carbohydrates(%)of rapeseed by 57.1%,11.1%and 15.7%,respec-tively compared to *** a result,the use of hydrogel polymer will be a viable and practical solution for increasing agricultural output under water deﬁcit stress situations.

关键词： Hydrogel oil osmolytes rapeseed yield water stress

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Analysis of MIF,FCGR2A and FCGR3A gene polymorphisms with susceptibility to pulmonary tuberculosis in Moroccan population

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Journal of Genetics and Genomics 2010年第4期37卷 257-264页

作者： Khalid Sadki Hoda Lamsyah Blanca Rueda ELmahfoud Akil abderrahim.sadak Javier Martin Rajae El Aouad Human Genomic Unit Laboratory of ImmunologyNational Institute of Hygiene Department of Biology Faculty of SciencesUniversity Mohammed V-Rabat Agdal 10000 Department of Immunology Instituto de Parasitología y Biomedicina López-NeyraCSIC National Reference Laboratory of Tuberculosis National Institute of Hygiene Immunology and Virology Department National Institute of Hygiene

In order to investigate the influence of functional polymorphisms of macrophage migration inhibitory factor （M/F）, Fcg receptors CD16A （FCGR3A） and CD32A （FCGR2A） genes on susceptibility to pulmonary tuberculosis （PTB） in the Moroccan population, we analyzed 123 patients with PTB and 154 healthy controls. The genotyping for M/F-173 （G/C）（rs755622）, FCGR2A- 131H/R （rs 1801274） and FCGR3A-158V/F （rs396991） was carried out using TaqMan SNP Genotyping Assay method. We found a statistically significant in- crease of the MIF-173CC homozygote genotype and M/F-173＂C allele frequencies in PTB patients compared with healthy controls （17.07% versus 5.84%, P = 0.003; and 35.37% versus 26.30%, P = 0.02; respectively）. In contrast, no association was observed between FCGR2A-131H/R and FCGR3A-158V/F polymorphisms and tuberculosis disease. Our finding suggests that M/F-173℃ variant may play an important role in the development of active tuberculosis.

关键词： FCGR MIF polymorphism susceptibility pulmonary tuberculosis Moroccan

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Selecting accurate post-elimination monitoring tools to prevent reemergence of urogenital schistosomiasis in Morocco:a pilot study

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Infectious Diseases of Poverty 2017年第1期6卷 664-672页

作者： Abdelaali Balahbib Fatima Amarir Paul L.A.M.Corstjens Claudia Jde Dood Govert Jvan Dam Amina Hajli Meryem Belhaddad Bouchra El Mansouri abderrahim.sadak Mohamed Rhajaouim El Bachir Adlaoui National Reference Laboratory for Schistosomiasis and Malacology National Institute of HygieneAgdalRabatMorocco Faculty of Science Laboratory of Zoology and General BiologyAgdalRabatMorocco Laboratory of Parasitology and Malacology Institute of Nursing Professions and Health TechniquesCasablancaMorocco Department of Molecular Cell Biology Leiden University Medical CenterLeidenThe Netherlands Department of Parasitology Leiden University Medical CenterLeidenThe Netherlands

Background:After alleged stop of transmission of schistosomiasis and further down the line in post elimination settings,sensitive tools are required to monitor infection status to prevent potential *** Rahala,where transmission cycle of Schistosoma haematobium is interrupted since 2004 but where 30%of snails are still infected by ***,potential human *** infection can’t be *** methods based on egg-counts do not provide the required sensitivity,antibody or antigen assays are envisaged as the most appropriate tools for this type of ***:In this pilot study,the performances of three assays were compared:two commercially available antibody tests(ELISA and haemagglutination format)indicating exposure,and an antigen test(lateral flow strip format)demonstrating active *** 37 recruited study participants resided in Rahala(Akka,province Tata,Morocco).Participants had been diagnosed and cured from schistosomiasis in the period between 1983 and *** 2015 these asymptomatic participants provided fresh clinical samples(blood and urine)for analysis with the aforementioned diagnostics ***:No eggs were identified in the urine of the 37 *** haemagglutination test indicated 6 antibody positives whereas the ELISA indicated 28 antibody positives,one indecisive and one false *** and haemagglutination results matched for 18 individuals,amongst which 5 out of 6 haemagglutination *** the antigen test(performed on paired serum and urine samples),serum from two participants(cured 21 and 32 years ago)indicated the presence of low levels of the highly specific Schistosoma circulating anodic antigen(CAA),demonstrating low worm level infections(less than 5 pg/ml corresponding to probably single worm pair).One tested also CAA positive with *** indicated the presence of human anti-Schistosoma antibodies in these two CAA positive cases,haemagglutination results were ***:To pre

关键词： Schistosomiasis Transmission stop Elimination Monitoring Antibody test Antigen test Circulating Anodic Antigen(CAA) Active infection

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Alleviation of Adverse Effects of Salt Stress in Wheat Cultivars by Foliar Treatment with Antioxidant 2—Changes in Some Biochemical Aspects, Lipid Peroxidation, Antioxidant Enzymes and Amino Acid Contents

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Agricultural Sciences 2014年第13期5卷 1269-1280页

作者： Ebtihal M. Abd Elhamid Mervat Sh. sadak Medhat M. Tawfik Botany Department Division of Agricultural and Biology National Research Centre Cairo Egypt Field Crops Research Department Division of Agricultural and Biology National Research Centre Cairo Egypt

Ascobin (compound composed of ascorbic acid and citric acid) is considered one of exogenous protectants which may alleviate the harmful effects of salinity stress. Pot experiments were performed at the screen greenhouse of National Research Centre, Cairo, Egypt to study the effect of foliar treatment of two cultivars of wheat plant with different concentrations of ascobin (0, 200, 400 and 600 mg/l) on some biochemical parameters, antioxidant enzymes, element contents and amino acid constituents of two cultivars of wheat plant grown under different salinity levels (0.0, 3000 and 6000 mg/l) in 2011/2012 and 2012/2013. Salinity with different concentrations levels increased phenolic compounds contents of the two wheat cultivars. The activities of antioxidant enzymes (SOD, CAT, POD, PPO, AXP and GR) dramatically increased due to salinity stress. Amino acid content was increased in cultivar Sids 1, while the content was decreased in cultivar Giza 168 in all salinity treatments. Increments in the above mentioned parameters compared to the untreated plants at normal and stressed conditions. The magnitude of increments was much more pronounced in response to 600 mg/l of ascobin. It could be concluded that, foliar treatment of wheat cultivars with ascobin could partially alleviate the harmful effect of salinity especially at the lower levels of salinity of the two cultivars of wheat at most of the studied parameters.

关键词： Amino Acid Antioxidant Enzymes Ascobin Phenolic Contents Salinity Wheat

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