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Chemical Characterization and Insecticidal Activity of Ethyl Acetate and Dichloromethane Extracts of Drypetes gossweileri against Sitophilus zeamais, Tribolium castaneum and Rhyzopertha dominica

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Journal of Life Sciences 2013年第10期7卷 1030-1040页

作者： aba toumnou lucie Seck Dogo Lakouetene Didier Ponel Beranger Bolevane Ouantinam Serge Florent Gueye Momar Talla Traore Anna Namkosserena Salomon Noba Kandioura Sembene Mbacke Syssa-Magale Jean-Laurent Regional Centre for Studies on the Improvement of Plant Adaptation to Drought Thies 3320 Senegal Regional Centre for Ecotoxicology Studies and Chemical Environment Security (CERES-Locustox) Dakar 3300 Senegal Department of Natural sciences University of Bangui Bangui 908 Central Africa Republic Institut of Food Technology Dakar 2765 Senegal Regional Pole of Applied Research to Development Farming Systems in Central Africa (PtL4SAC) N 'Djamena 764 Chad Department of Plant Biology Cheikh Anta Diop University Dakar 5005 Senegal Department of Animal Biology Cheikh Anta Diop University Dakar 5005 Senegal

The use of botanical pesticides in pest management during storage against insects is often encouraged because synthetic insecticides produce undesired effects. Bark powder ofDrypetes gossweileri is used by farmers in pest management in Central Africa Republic. Hexane, dichloromethane, ethyl acetate and methanol were used for extraction bark powder by maceration. Insecticidal activity of the extracts was tested on Sitophilus zeamais, Tribolium castaneum and Rhy_-opertha dominica. The mortality rate, the number of surviving insects and the number of emerged insects were measured variables. Calculated mortality was obtained by applying Aboth＇s formula. The variables are submitted to variance analysis, model with four factors （plants, insects, doses and time） in Minitab 14. The results of analysis showed that the mortality rate has a very highly significant variation following extracts, doses, insects and time considered （P 〈 0.001）. Extracts with ethyl acetate and dichloromethane of D. gossweileri are effective against S. zeamais and R. dominica at high doses （1, 0.5, 0.250 g/10 mL of solvent）. These effective extracts were characterized by gas chromatography coupled with mass spectrometry. Molecules such as l-isothiocyanatomethyl, thiocyanic acid, phenylmethyl ester and other were characterizated. These results may consolidate traditional use olD. gossweileri in pest management.

关键词： Control stored grains pests' management Drypetes gossweileri.

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The Efficient Role of Dermestes in Damage of Fish in Bangui (Central African Republic)

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Advances in Entomology 2015年第3期3卷 111-117页

作者： Bolevane-Ouantinam Serge Florent aba-toumnou lucie Gondi Armelle Judith Wango Solange Patricia Zinga Innocent Namkosséréna Salomon Lakouèténé Didier Ponel-Béranger Kongueret Ernest Semballa Silla Laboratory of Applied Animal Biology and Biodiversity University of Bangui Bangui Central African Republic Laboratory of Biological and Agronomical Sciences for Development Bangui Central African Republic Regional Pole of Applied Research to Development Farming Systems in Central Africa (PRASAC) Djamena Chad Central African Republic Institute of Agronomical researches (ICRA) Bangui Central African Republic

A bad conservation of food is recognized as being one of the critical constraints upon food security among resource poor population across Africa. We have evaluated the traditional management of pests in fish in Central African Republic. The data were analyzed by a factor analysis of correspondence. The Group I contained sensitive species of fish to the attacks of Dermetes frishii, Dermetes lardarius and Dermestes carnivorus. There were: Labeocoubie, Mormyrus deliciosus, Polydactylus quadrifilis, Auchenoglanis occidentalis, Synodontis nigrita, Hydrocynus forskalli, Districhodus rostratus, Hydrocynus goliath and Mormyrusrume. The group II contained sensitive species of fishes to the attacks of D. maculatus. There were: Cyprinus carpio, Malapterurus electricus, Oreochromis mossambicus, Barbus occidentalis and Oreochromis mossambicus. Clariasgariecilus is the mostsensitive of dried fish to the attacks of Dermestes. Oreochromis mossambicus, Mormyrusrume and Synodontis nigrita are the last sensitive of dried fish to the attacks of Dermestes. The results showed that the tradictional management of dried fish in Central African Republic couldn’t be efficient for reducing the development of Dermestes.

关键词： Traditonal Management Pests Dried Fish

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Pest Management of Storage Pests Using Extracts of Afrostyrax lepidophyllus against Sitophilus zeama＇（s, Tribolium castaneum and Rhyzopertha dominica

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Journal of Agricultural Science and Technology(B) 2014年第1期4卷 58-66页

作者： aba toumnou lucie Gueye Momar Talla Bolevane Ouatinam Serge Florent Traore Anna Gueye Sabelle Lakouetene Didier Ponel Beranger Namkosserena Salomon Ndoye Ousmane Syssa-Magale Jean-Lauren Noba Kandioura Sembene Mbacke Seck Dogo Faculty of Sciences University of Bangui Bangui Central Africa Republic lnstitut of Food Technology Dakar 3300 Senegal Regional Centre for Ecotoxicology Studies and Chemical Environment Security (CERES-Locustox) Dakar 3300 Senegal Regional Pole of Applied Research to Development Farming Systems in Central Africa (PRASAC) Bangui Central Africa Republic West and Central African Council for Agricultural Research and Development Dakar 18523 Senegal Faculty of Sciences Cheikh Anta Diop University Dakar 5005 Senegal

The use of botanical pesticides in pest management during storage against insects is often encouraged because synthetic insecticides produce multiple side-effects on human health and environment. Insecticidal activity of the hexane, dichloromethane, ethyl acetate and methanol extracts of bark ofAfrostyrax lepidophyllus was tested on Sitophilus zeama~：~, Tribolium castaneum and Rhy-opertha dominica. The mortality rate was measured variable. The results of analysis showed that the mortality rate has a very highly significant variation following extracts, doses, insects and time considered （P 〈 0.001）. Extracts with ethyl acetate and hexane of A. lepidophyllus are effective against S. zeamais at the highest doses （1 g/10 mL and 0.5 g/10 mL of solvent）. These effective extracts werc characterized by gas chromatography coupled with mass spectrometry. Molecules such as thiocyanic acid, ethyl ester： dimetbylditbiophosphinic acid and other were characterizated. These results may consolidate traditional use ofA. lepidophylh^s in pest management.

关键词： Control stored grains pests management Afrostyrax lepidophyllus.

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Biodiversity of Edible Caterpillars of Imbrasia and Host Plants in Boukoko (Central African Republic)

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Advances in Entomology 2024年第1期12卷 38-55页

作者： lucie aba-toumnou Solange-Patrica Wango Denis Beina Jean-Pierre Moalbaye Gorgon Igor Touckia Rufin Reo-Ndouba Felix Allah-Barem Olga Diane Yongo Laboratory of Biological and Agronomical Sciences for Development Faculty of Sciences University of Bangui Bangui Central African Republic Laboratory of Applied Animal Biology and Biodiversity University of Bangui Bangui Central African Republic Center of Studies and Research on Pharmacopoeia and Traditional African Medicine (CERPHAMETA) University of Bangui Bangui Central African Republic Plant and Fungal Biodiversity Laboratory Faculty of Science University of Bangui Bangui Central African Republic Higher Institute for Rural Development (ISDR) University of Bangui Mba&iumlki Central African Republic Central African Republic Institute of Agriculture Research Bangui Central African Republic

In Central African Republic, edible caterpillars of Imbrasia gender are income of rural populations, present an opportunity to improve food security and belonging to the category of Non-wood forest products. The goal of this study is to document the ecology of caterpillars of genre Imbrasia in relation to its host plants in Boukoko. The questionnaire was addressed to the actors of the collection and consumption of caterpillars in this Commune, to their local knowledge concerning this food resource as well as the plants of their locality on which caterpillars are collected. The survey took place in September to October 2022 and as a general rule, the 120 respondents were elderly people whose age range varied from 35 to 70 years old. Plant samples are collected in their natural environment. Based on local names;herbaria are mounted, plants photographed and samples collected. The types of edible caterpillars have been inventoried in the field in collaboration with the local populations who consume them. Based on local names, the caterpillars are captured manually from morphological traits and then stored in bottles containing 70% ethanol. The specimens were identified. The data was entered into the Excel spreadsheet which also made it possible to produce histograms with the frequencies of the plant species most valued by the different caterpillars. A Principal Component Analysis (PCA) was then performed using R software to highlight the correlation between caterpillars and host plants. The main economic importance trees hosting edible caterpillars of Imbrasia gender in Boukoko region are: Entandrophragma cylindricum, Pentaclethra macrophylla, Maesopsis eminii, Albizia ferruginea, Macaranga spinosa, Amphimas ferrugineus, Pentaclethra macrophylla, Racosperma auriculiforme, Eribroma oblonga. The Principal Component Analysis (PCA) carried out to assess the variability between the different species of caterpillars according to their host plants shows that the first two axes

关键词： Forest Degradation Losing Biodiversity of Consumed Insects Local Population

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Microsphere-assisted quantitative phase microscopy:a review

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Light(Advanced Manufacturing) 2024年第1期5卷 131-150页

作者： Vahid Abbasian Tobias Pahl lucie Hüser Sylvain Lecler Paul Montgomery Peter Lehmann Arash Darafsheh Department of Radiation Oncology Washington University School of Medicine in St.LouisSt.LouisMO 63110USA Imaging Science Program McKelvey School of EngineeringWashington University in St.LouisSt.LouisMO 63130USA Measurement Technology Group Faculty of Electrical Engineering and Computer ScienceUniversity of KasselWilhelmshöher Allee 7134121 KasselGermany ICube Research Institute INSA StrasbourgUniversitéde StrasbourgCNRS67000 StrasbourgFrance

Light microscopes are the most widely used devices in life and material sciences that allow the study of the interaction of light with matter at a resolution better than that of the naked *** microscopes translate the spatial differences in the intensity of the reflected or transmitted light from an object to pixel brightness differences in the digital ***,a phase microscope converts the spatial differences in the phase of the light from or through an object to differences in pixel *** microscopy,a phase-based approach,has found application in various *** interferometry has brought nanometric axial resolution,the lateral resolution in quantitative phase microscopy(QPM)has still remained limited by diffraction,similar to other traditional microscopy *** the resolution has been the subject of intense investigation since the invention of the microscope in the 17th *** the past decade,microsphere-assisted microscopy(MAM)has emerged as a simple and effective approach to enhance the resolution in light *** can be integrated with QPM for 3D label-free imaging with enhanced ***,we review the integration of microspheres with coherence scanning interference and digital holographic microscopies,discussing the associated open questions,challenges,and opportunities.

关键词： Phase microscopy Digital holographic microscopy Microsphere Resolution

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Distribution of atrial cardiomyopathy markers and association with atrial fibrillation detected after ischaemic stroke in the SAFAS study

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Stroke & Vascular Neurology 2024年第2期9卷 165-173,I0426,I0427页

作者： Romain Didier lucie Garnier Gauthier Duloquin Alexandre Meloux Audrey Sagnard Mathilde Graber Geoffrey Dogon Karim Benali Thibaut Pommier Gabriel Laurent Catherine Vergely Yannick Bejot Charles Guenancia Cardiology CHU Dijon BourgogneDijonFrance Dijon Stroke Registry Department of NeurologyUniversity Hospital Centre DijonDijonFrance PEC 2 Universitéde BourgogneDijonFrance Cardiology CHU Saint EtienneSaint EtienneFrance

Background Atrial cardiomyopathy(AC)is an emerging concept explaining the pathophysiology of cardioembolic strokes in absence of atrial fibrillation(AF).A definition based on the presence of electrical abnormality(P-wave terminal force in lead V1(PTFV1)>5000µV×ms),N-Terminal pro-B-type natriuretic peptide(NT pro BNP)>250pg/mL and/or indexed left atrial diameter(LADI)>3cm/m^(2)is currently tested in the ARCADIA(AtRial Cardiopathy and Antithrombotic Drugs In prevention After cryptogenic stroke)*** set out to estimate the prevalence of AC as defined in the ARCADIA trial,its determinants and its association with AF detected after stroke(AFDAS).Methods Stepwise screening for silent Atrial Fibrillation After Stroke(SAFAS)study prospectively included 240 ischaemic stroke *** markers were complete for 192 of them and 9 were not included in this analysis because AF had been diagnosed on *** A total of 183 patients were analysed,of whom 57%(104 patients)met the AC criteria(79 NT-proBNP,47 PTFV1,4 LADI).In the multivariate logistic regression,C reactive protein>3mg/L(OR(95%CI)2.60(1.30 to 5.21),p=0.007)and age(OR(95%CI)1.07(1.04 to 1.10),p34mL/m^(2),OR 2.35(CI 1.09 to 5.06)p=0029).Conclusion AC as defined in ARCADIA is mostly based on NT pro BNP elevation(76%of patients)and is associated with age and ***,AC was not independently associated with AFDAS at *** ARCADIA trial,which compares aspirin to apixaban in patients with embolic strokes of undetermined source with AC markers and must,therefore be analysed in the light of these limitations.

关键词： atrial cardiomyopathy prevention

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Sustaining forest biodiversity:Exploring the effect of long-term natural disturbance dynamics on contemporary lichen communities in primary forest ecosystems

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Forest Ecosystems 2024年第4期11卷 556-567页

作者： Rhiannon Gloor Marek Svitok Martin Mikolas Jenyk Hofmeister Josef Halda Pavel Janda Francesco Maria Sabatini lucie Zemanova Arne Buechling Daniel Kozak Matej Ferencik Michal Frankovic Martin Dusatko Miroslav Svoboda Department of Forest Ecology Faculty of Forestry and Wood SciencesCzech University of Life Sciences PragueKamycka 129Praha 6–Suchdol16500Czech Republic Faculty of Ecology and Environmental Sciences Technical University in ZvolenT.G.Masaryka 2496001ZvolenSlovakia Institute of Botany Plant Science and Biodiversity CenterSlovak Academy of SciencesDúbravska cesta 984523BratislavaSlovakia PRALES Odtrnovie 56301322RosinaSlovakia Faculty of Science University of Hradec KraloveHradecka 1285Hradec KraloveⅢ50003Czech Republic BIOME–Biogeography and Macroecology Department of BiologicalGeological and Environmental Sciences(BIGEA)Alma Mater Studiorum-University of Bolognavia Irnerio 42BolognaItaly

In this era of biodiversity loss and climate change,quantifying the impacts of natural disturbance on forest communities is imperative to improve biodiversity conservation *** and epixylic lichens are effective forest quality bioindicators,as they are generally long-lived organisms supported by continuity of specific forest structures and their associated microclimatic ***,how lichen communities respond to the effects of fluctuating historical disturbances remains *** a dendrochronological approach,this study investigates how natural disturbance dynamics indirectly influence various lichen community metrics in some of Europe's best-preserved primary mixed-beech *** modelling revealed that natural historical disturbance processes have decades-long effects on forest structural attributes,which had both congruent and divergent impacts on lichen community richness and *** species richness indirectly benefited from both historical and recent higher-severity disturbances via increased standing dead tree basal area and canopy openness respectively-likely through the presence of both pioneer and late-successional species associated with these ***-listed species richness showed a dependence on habitat continuity(old trees),and increased with disturbance-related structures(standing dead trees)whilst simultaneously benefiting from periods without severe disturbance events(old trees and reduced deadwood volume).However,if the disturbance occurred over a century in the past,no substantial effect on forest structure was ***,while disturbance-mediated forest structures can promote overall richness,threatened species appear vulnerable to more severe disturbance events-a concern,as disturbances are predicted to intensify with climate ***,the high number of threatened species found reinforce the critical role of primary forest structural attributes for biodiversity ***

关键词： Mixed-beech forests Species richness Old-growth forests Carpathian mountains Biodiversity conservation Dendroecology Fagus sylvatica

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Theoretical Study of Benzothiazole and Its Derivatives: Molecular Structure, Spectroscopic Properties, NBO, MEP and TD-DFT Analyses

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Journal of Materials Science and Chemical Engineering 2024年第3期12卷 31-50页

作者： Konaté Abdoulaye Bédé Affoué lucie Ouattara Lamoussa Koné Soleymane Bamba Kafoumba Laboratory of Constitution and Reaction of Matter University Félix Houphou&#235t-Boigny Abidjan Ivory Coast Department of Marine Sciences University of San-Pedro San-Pedro Ivory Coast Laboratory of Thermodynamics and Physico-Chemistry of Environment University Nangui Abrogoua Abidjan Ivory Coast

Benzothiazole (BTH) and its derivatives are organic molecules with biologic actions. Because of their many applications, they are produced on a massive scale and used in a number of environmental compartments. Their discharge into water produces environmental problems, exposing our environment to public health problems. A solution that can contribute to their deterioration is becoming a necessity. For this reason, a conceptual analysis of the reactivity of benzothiazole and four of its compounds was undertaken in order to investigate certain aspects of their biodegradability. A theoretical investigations of the compounds studied were conducted in the gas and water phases with the most widely used density functional theory method, Becke-3-Parameter-Lee-Yang-Parr (B3LYP) with 6-31G+ (d, p) basis. Reactivity study calculated global indices of reactivity revealed that 2-SCH3_BTH is the most reactive. Dipole moment values analysis reveals that 2-NH2_BTH is the most soluble in water, while the lipophilicity shows that 2-NH2_BTH is the most hydrophilic compound. Thermodynamic parameters values reflect that reactions are respectively exothermic and spontaneous. By analyzing an Electrostatic Molecular Potential (EMP) map, researchers can pinpoint reactive sites on a molecule and anticipate its reactivity. This assessment is further enhanced by incorporating global and local reactivity descriptors. Additionally, an exploration of frontier molecular orbitals offers valuable insights into the molecule’s charge transfer characteristics. Moreover, a combined examination of internal and external molecular interactions unveils hyperconjugative interactions arising from charge delocalization, as elucidated through natural bond orbital (NBO) analysis.

关键词： Benzothiazole Reactivity DFT/B3LYP Stability TD-DFT

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Prevalence of Enteric Pathogens Associated with Infections among Table Egg Consumers in Some Primary Health Establishments in the Center Region of Cameroon

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Advances in Infectious Diseases 2024年第3期14卷 606-619页

作者： Mariane lucie Ngueguim Bernard Viban Tangwa Victor Ngu Ngwa Albert Ngakou Abdoulmoumini Mamoudou School of Veterinary Medicine and Sciences University of Ngaoundere Ngaoundere Cameroon Department of Veterinary Medicine University of Buea Buea Cameroon Faculty of Sciences University of Ngaoundere Ngaoundere Cameroon

Method: In Cameroon limited data are available regarding the prevalence of enteric bacteria associated with table egg consuming infections. As such, a situational-based study was performed in patients with complains of stomach disorders after egg consumption. Data related to sociodemographic characteristics and other factors were collected using a structured based questionnaire. Stool culture of utmost importance in stomach disorders patients and serum were collected for typhoid serological test. Results: A total of 207 participants took part in the survey, Results indicated nontyphoidal Salmonella infections were highest in the 3 areas of study with Mfoundi (73.44%) having the highest level of infection compared to other bacterial infection. other enteric bacteria associated to this infection were E. coli serotype 157, Aeromonas, Citrobacter freundii, Enterobacter cloaca and typhi salmonella. Meanwhile salmonelosis caused by typhic salmonella had highest prevalence in the Lekie Division (13.11%) as a result of poor hygienic practices associated with the conservation and preparation of eggs, Stool culture was observed to detect more positive cases in the diagnosis of typhoid fever than Widal test, but with no statistically significant (p > 0.05) difference between the stool culture and Widal test in the 3 areas of study. Conclusion: this study revealed that egg consumers are pruned to enteric bacterial and salmonella infections depending on how and where egg is consumed.

关键词： Enteric Pathogens Table Eggs Consumption Center Region Cameroon

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Comparative Study of the Widal Test against Stool Culture in the Diagnosis of Suspected Cases of Typhoid Fever in Some Low Income Communities in the Adamawa Region of Cameroon

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Journal of Biosciences and Medicines 2024年第6期12卷 178-190页

作者： Mariane lucie Ngueguim Bernard Viban Tangwa Victor Ngu Ngwa Jules Romain Ngueguim Abdoulmoumini Mamoudou School of Veterinary Medicine and Sciences University of Ngaoundere Ngaoundere Cameroon Department of Veterinary Medicine University of Buea Buea Cameroon Institute de Recherhe Agricole pour le Development (IRAD) Ngaoundere Cameroun

Introduction: Infectious diseases constitute a major concern of public health in developing countries. Facilities and well trained staff have been shown to be one of the major obstacles in the rapid and quality diagnosis of these diseases. As such, we carried out an analysis to compare the Widal test and stool culture to identify febrile patients with Salmonella infection. Method: A cross sectional study was conducted to diagnose salmonella infection with out-patients who demonstrated signs of salmonella infection. Serum was harvested from blood collected from 368 (Vina = 234, Mayo Banyo 65, and Djerem = 69) patients accompanied by stool, Widal test was conducted on the spot and stool was taken to a reference laboratory for culture using standard microbiological methods, sociological set up was calculated in percentages, prevalence was calculated using excel while statistical difference was calculated using SPSS. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated to compare the Widal test against stool culture. Results: A total of 368 (50.8% females and 49.2% males) participants took part in the survey. Salmonella prevalence (66.24%) in stool culture in the Vina division was significantly different (p 0.05). The sensitivity,specificity, PPV, and NPV of slide agglutination test against stool culture varied from different areas (Vina: 51.6%, 73.62%, 79.21% and 43.61%;Mayo Banyo: 60.53%, 77.78%, 79.31% and 58.33%;Djerem: 53.18%, 83.73% 73.91% and 67.39%) respectively. Slide agglutination test has a fair agreement with the stool culture (kappa, Vina = 0.202;Mayo Banyo = 0.37 and Djerem = 0.38). Conclusion: Generally, in the three areas of study, the Widal test had a fair correlation with the stool culture;This means the Widal test should not be used alone but in combination with stool culture in the detection of salmonella infections.

关键词： Salmonella Infections Stool Culture Widal Test Adamawa Region Cameroon

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