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Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens)

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Animal Nutrition 2022年第2期9卷 143-158页

作者： Xing Lu Dong-Fang Deng Fei Huang Fabio Casu Emma Kraco Ryan J.Newton merry Zohn Swee J.Teh aaron m.watson Brian Shepherd Ying ma mahmound A.O.Dawood Lorena m.Rios mendoza School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA South Carolina Department of Natural Resources CharlestonSC 29412USA USDA/ARS/School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA School of Veterinary medicine Department of AnatomyPhysiologyand Cell BiologyUniversity of CaliforniaDavisCA 95616USA Department of Natural Sciences Marine Resources Research InstituteUniversity of WisconsinSuperiorWI 54880USA Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhanChina Fisheries College Jimei UniversityXiamenChina. Faculty of Agriculture Kafrelsheikh UniversityKafr El-SheikhEgypt.

microplastics are emergent contaminants threatening aquatic organisms including aquacultured *** study investigated the effects of high-density polyethylene(HDPE,100 to 125 mm)on yellow perch(Perca flavescens)based on integrative evaluation including growth performance,nutritional status,nutrient metabolism,fish health,and gut microbial *** test diets(0,1,2,4,or 8 g HDPE/100 g diet)containing 41%protein and 10.5%lipid were fed to juvenile perch(average body weight,25.9±0.2 g;n=15)at a feeding rate of 1.5%to 2.0%body weight *** feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per *** mortality or HDPE accumulation in the fish was found in any *** gain and condition factor of fish were not significantly impacted by HDPE(P>0.05).Compared to the control group,fish fed the 8%HDPE diet had significantly decreased levels of protein and ash(Pmatic index value,hepatocyte size,and liver glycogen level were increased,but lipid content was reduced in the liver *** to the control treatment,fish fed the 8%HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis,pyruvate metabolism,and carnitine *** enterocyte necrosis was documented in the foregut of fish fed the 2%or 8%HDPE diet;and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8%HDPE *** fed the 2%HDPE diet harbored different microbiota communities compared to the control *** study demonstrates that HDPE ranging from 100 to 125 mm in feed can be evacuated by yellow perch with no impact on ***,dietary exposure to HDPE decreased whole fish nutrition quality,altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow *** results indicate that extende

关键词： Dietary exposure Intestinal microbiota Liver metabolomics microplastics Nutrient composition Yellow perch

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Evaluation of a hepatic biomarker of nutritional imbalance in juvenile red drum(Sciaenops ocellatus)fed 60%soybean meal-based diets using NmR-based metabolomics

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Animal Nutrition 2024年第1期16卷 45-61页

作者： Fabio Casu aaron m.watson Justin Yost T.Gibson Gaylord Daniel W.Bearden michael R.Denson marine Resources Research Institute South Carolina Department of Natural Resources217 Fort Johnson RoadCharlestonSC 29412USA Bozeman Fish Technology Center United States Fish and Wildlife Service4050 Bridger Canyon RoadBozemanMT 59715USA marine Biochemical Sciences Group Chemical Sciences DivisionNational Institute of Standards and TechnologyHollings Marine Laboratory331 Fort Johnson RoadCharlestonSC 29412USA National Institute of Standards and Technology(NIST) Hollings Marine Laboratory331 Fort Johnson RoadCharlestonSC 29412USA National Oceanic and Atmospheric Administration(NOAA) Hollings Marine Laboratory331 Fort Johnson RoadCharlestonSC 29412USA

A 12-week feeding trial with juvenile red drum(Sciaenops ocellatus) fed high-soybean meal(SBm) diets was conducted to investigate a putative biomarker of nutritional imbalance, N-formimino-L-glutamate(FIGLU). Three fishmeal-free, 60% SBm pelleted diets(named B12, Fol, and met, respectively) were tested to evaluate the effects on growth performance and tissue metabolite profiles of supplementation of vitamin B12(0.012 mg/kg), folate(10 mg/kg), methionine(1 g/kg) respectively, above basal supplementation levels. A fourth SBm-based diet(named B12/Fol/met) was formulated with a combination of B12, folate, and methionine to attain the above-mentioned target concentrations. A fifth 60% SBm diet(named FWS) with methionine supplementation(1 g/kg above basal supplementation levels), enriched with taurine, lysine and threonine as well as minerals, was also tested. This diet contained formulation targets and additives which have allowed for replacing fishmeal with plant proteins in rainbow trout feeds. Control diets included a fishmeal-based diet(named Fm), an unsupplemented basal 60% SBm diet(named SBm60), and a “natural” diet(named N) made up of equal parts of fish(cigar minnows), squid and shrimp as a positive reference for growth performance. Formulated feeds contained approximately 37% total crude protein, approximately 14% total crude lipid and were energetically balanced. Standard growth performance metrics were measured, and tissues(liver, muscle) were collected at week 12 to evaluate diet-induced metabolic changes using nuclear magnetic resonance(NmR)-based *** results show that the FWS diet outperformed all other SBm diets and the Fm diet under all performance metrics(Pm diets and the Fm diet. Fish fed the FWS diet and the met diet showed lower hepatic levels of FIGLU compared with the other SBm-based diets(Pmong the different supplementatio

关键词： Aquaculture metabolomics Nuclear magnetic resonance Red drum Soybean meal N-Formimino-L-glutamate

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The role of estradiol in male reproductive function

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Asian Journal of Andrology 2016年第3期18卷 435-440,I0009页

作者： michael Schulster aaron m Bernie Ranjith Ramasamy Department of Urology New York-Presbyterian Hospital Weill Cornell Medical College New York USA Department of Urology Miller School of Medicine University of Miami Miami FL USA.

Traditionally, testosterone and estrogen have been considered to be male and female sex hormones, respectively. However, estradiol, the predominant form of estrogen, also plays a critical role in male sexual function. Estradiol in men is essential for modulating libido, erectile function, and spermatogenesis. Estrogen receptors, as well as aromatase, the enzyme that converts testosterone to estrogen, are abundant in brain, penis, and testis, organs important for sexual function. In the brain, estradiol synthesis is increased in areas related to sexual arousal. In addition, in the penis, estrogen receptors are found throughout the corpus cavernosum with high concentration around neurovascular bundles. Low testosterone and elevated estrogen increase the incidence of erectile dysfunction independently of one another. In the testes, spermatogenesis is modulated at every level by estrogen, starting with the hypothalamus-pituitary-gonadal axis, followed by the Leydig, Sertoli, and germ cells, and finishing with the ductal epithelium, epididymis, and mature sperm. Regulation of testicular cells by estradiol shows both an inhibitory and a stimulatory influence, indicating an intricate symphony of dose-dependent and temporally sensitive modulation. Our goal in this review is to elucidate the overall contribution of estradiol to male sexual function by looking at the hormone＇s effects on erectile function, spermatogenesis, and libido.

关键词： estrogen testosterone spermatogenesis erectile function estrogen receptor aromatase

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Experimental Analysis of Atypically Long Finned Oscillating Heat Pipe for Ventilation Waste Heat Recovery Application

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Journal of Thermal Science 2020年第3期29卷 667-675页

作者： mAHAJAN Govinda CHO Heejin SmITH aaron THOmPSON Scott m. Rheem manufacturing MontgomeryAL 36109USA Department of mechanical Engineering Mississippi State UniversityMsissippi StateMS 39762USA Department of mechanical Engineering Auburn UniversityAubumnAL 36849USA

Oscillating heat pipes(OHP)which are constructed from a serpentine-arranged capillary tube possess a desirable aerodynamic form factor and provide for relatively high heat transfer rates via cyclic evaporation and condensation of an encapsulated working fluid with no internal wicking structure *** last two decades,OHP has been extensively investigated for its potential application in thermal management of various *** study presents an experimental investigation on the heat transfer performance of an atypically long finned *** heat transfer performance of the proposed OHP was analyzed and compared with a bare tube OHP with similar overall *** show that a unit row of finned OHP filled with n-pentane with fill ratio of 70%can recover up to(400±40)W of heat from a typical waste exhaust air *** additional pressure drop due to fins was estimated to be(6.8±2)Pa resulting in an increase of 1–2 W of fan power *** average heat recovery rate via finned OHP was found to be almost 80%more than bare tube OHP filled with same working fluid with same fill ratio.

关键词： oscillating heat pipe waste heat recovery ventilation

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Cytokinin-mediated nuclear shuttling of mYB3R4 regulates cell division

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molecular Plant 2021年第5期14卷 720-721页

作者： m.Arif Ashraf aaron m.Rashotte Department of Biology University of Massachusetts AmherstAmherstMA 01003USA Department of Biological Sciences Auburn UniversityAuburnAL 36849USA

The plant hormone cytokinin,an N6-substituted adenine derivative,was first discovered by Carlos miller and Folke Skoog nearly 70 years ago as being able to promote cell *** then,there have been great advances in our understanding of cytokinin biosynthesis,perception and signaling,and expanded roles across plant growth and development(Schaller et al.,2014).However,the molecular mechanism underlying cytokinin-mediated cell division for which this hormone was first identified is still poorly *** ***-mediated nuclear import of mYB3R4.

关键词： Figure expanded mediated

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Estimating underground mine ventilation friction factors from low density 3D data acquired by a moving LiDAR

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International Journal of mining Science and Technology 2018年第4期28卷 650-655页

作者： Curtis watson Joshua marshall mining Systems Laboratory The Robert M.Buchan Department of Mining Queen's University

Ventilation system analysis for underground mines has remained mostly unchanged since the Atkinson method was made popular by mc Elroy in 1935. Data available to ventilation technicians and engineers is typically limited to the quantity of air moving through any given heading. Because computer-aided modelling, simulation, and ventilation system design tools have improved, it is now important to ensure that developed models have the most accurate information possible. This paper presents a new technique for estimating underground drift friction factors that works by processing 3 D point cloud data obtained by using a mobile Li DAR. Presented are field results that compare the proposed approach with previously published algorithms, as well as with manually acquired measurements.

关键词： mine ventilation mine design mobile mapping Roughness estimation Friction factors LiDAR3D point clouds

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Importance of being Nernst: Synaptic activity and functional relevance in stem cell-derived neurons

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World Journal of Stem Cells 2015年第6期7卷 899-921页

作者： aaron B Bradford Patrick m mcNutt Cell/molecular Biology Branch US Army Medical Research Institute of Chemical DefenseAberdeen Proving Grounds-EAMD 21010United States

Functional synaptogenesis and network emergence are signature endpoints of neurogenesis. These behaviors provide higher-order confirmation that biochemical and cellular processes necessary for neurotransmitter release, post-synaptic detection and network propagationof neuronal activity have been properly expressed and coordinated among cells. The development of synaptic neurotransmission can therefore be considered a defining property of neurons. Although dissociated primary neuron cultures readily form functioning synapses and network behaviors in vitro, continuously cultured neurogenic cell lines have historically failed to meet these criteria. Therefore, in vitro-derived neuron models that develop synaptic transmission are critically needed for a wide array of studies, including molecular neuroscience, developmental neurogenesis, disease research and neurotoxicology. Over the last decade, neurons derived from various stem cell lines have shown varying ability to develop into functionally mature neurons. In this review, we will discuss the neurogenic potential of various stem cells populations, addressing strengths and weaknesses of each, with particular attention to the emergence of functional behaviors. We will propose methods to functionally characterize new stem cell-derived neuron(SCN) platforms to improve their reliability as physiological relevant models. Finally, we will review how synaptically active SCNs can be applied to accelerate research in a variety of areas. Ultimately, emphasizing the critical importance of synaptic activity and network responses as a marker of neuronal maturation is anticipated to result in in vitro findings that better translate to efficacious clinical treatments.

关键词： Synapses Neurotransmission In vitrotechniques Induced pluripotent stem cells Neuronalnetworks Neurogenesis Neural stem cells Embryonicstem cells Induced neurons

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Cross-talk between the thyroid and liver:A new target for nonalcoholic fatty liver disease treatment

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World Journal of Gastroenterology 2013年第45期19卷 8238-8246页

作者： Yue-Ye Huang aaron m Gusdon Shen Qu Department of Endocrinology and metabolism Shanghai 10th People’s Hospital School of Medicine Tongji University Department of Neurology and Neuroscience Weill Cornell Medical College New York NY 10065 United States Department of Endocrinology and metabolism Nanjing Medical University

Nonalcoholic fatty liver disease(NAFLD)has been recognized as the most common liver metabolic disease,and it is also a burgeoning health problem that affects one-third of adults and is associated with obesity and insulin resistance *** hormone(TH)and its receptors play a fundamental role in lipid metabolism and lipid accumulation in the *** is found that thyroid receptor and its isoforms exhibit tissue-specific expression with a variety of ***β1 is predominantly expressed in the brain and adipose tissue and TRβ2 is the major isoform in the liver,kidney and *** have different functions and play important roles in lipid ***,there are many studies on the treatment of NAFLD with TH and its *** review here that thyroid hormone and TR are a potential target for pharmacologic *** metabolism and lipid accumulation can be regulated and reversed by TH and its analogues.

关键词： Thyroid hormone Thyroid hormone receptor Nonalcoholic fatty liver disease Obesity

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Equine-assisted interventions for veterans with servicerelated health conditions: a systematic mapping review

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military medical Research 2020年第2期7卷 174-189页

作者： Adam R.Kinney aaron m.Eakman Rebecca Lassell Wendy Woo Department of Occupational Therapy Colorado State UniversityFort CollinsCO 80523USA Temple Grandin Equine Center Department of Animal SciencesColorado State UniversityFort CollinsCO 80523USA

Background:Evidence-based treatments for service-related health conditions such as posttraumatic stress disorder(PTSD),depression,and traumatic brain injury(TBI)are not effective for all ***-assisted interventions are emerging as an additional treatment modality,but little is known regarding the safe and effective delivery of these *** study aimed to describe the following features of the body of literature concerning equineassisted interventions among veterans:1)veterans who have participated in equine-assisted interventions;2)specific characteristics of equine-assisted interventions in veterans;and 3)the specific characteristics of research on equineassisted interventions in ***:We conducted a systematic mapping review of peer-reviewed literature reporting on equine-assisted interventions among veterans between 1980 and *** of nine databases yielded 3336 unique records,six of which met the inclusion criteria and were *** relevant to the study aims were extracted and ***:Equine-assisted interventions among veterans disproportionately targeted psychosocial outcomes and yielded promising *** detailed methods of EAI varied in the reported studies,ranging from communicating with the horse to mounted *** was also great diversity in outcome *** state of theoretical development regarding the mechanisms by which equine-assisted interventions benefit the veteran population is currently *** provided insufficient detail with respect to the description of the intervention,reasons for attrition,and the dose-response ***:Scientific development of equine-assisted interventions targeting psychosocial outcomes among veterans is warranted to establish their *** outcomes should be expanded,including outcomes more closely aligned with the nature of polytraumatic *** research must also emphasize the theoretical develo

关键词： Veterans Service-related injuries Equine-assisted interventions Posttraumatic stress disorder Systematic mapping review

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Critical minimum temperature limits xylogenesis and maintains treelines on the southeastern Tibetan Plateau

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Science Bulletin 2017年第11期62卷 804-812页

作者： Xiaoxia Li Eryuan Liang Jozica Gricar Sergio Rossi Katarina Cufar aaron m. Ellison Key Laboratory of Alpine Ecology and Biodiversity Key Laboratory of Tibetan Environment Changes and Land Surface Processes Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100101 China CAS Center for Excellence in Tibetan Plateau Earth Sciences Beijing 100101 China Sloven ian Forestry Institute Department of Yield and Silviculture Vecna pot 2 S1-1000 Ljubljana Slovenia University of Quebec in Chicoutimi Departement des Sciences Fondamentales 555 Boulevard de l'Universite Chicoutimi (QC) G7H2B1 Canada Key Laboratory of Vegetation Restoration and management of Degraded Ecosystems Guangdong Provincial Key Laboratory of Applied Botany South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China University of Ljubljana Biotechnical Faculty Department of Wood Science and Technology Jamnikarjeva 101 S1-1000 Ljubljana Slovenia Harvard Forest Harvard University 324 North Main St. Petersham MA 01366 USA

Physiological and ecological mechanisms that define treelines are still debated. It has been suggested that the absence of trees above the treeline is caused by low temperatures that limit growth. Thus, we hypothesized that there is a critical minimum temperature （CTmin） preventing xylogenesis at treeline. We tested this hypothesis by examining weekly xylogenesis across three and four growing seasons in two natural Smith fir （Abies georgei var. srnithii） treeline sites on the southeastern Tibetan Plateau. Despite differences in the timing of cell differentiation among years, minimum air temperature was the dominant climatic variable associated with xylem growth; the critical minimum temperature （CTmin） for the onset and end of xylogenesis occurred at 0.7 ±0.4 ℃. A process-based modelling chronology of tree-ring formation using this CTmin was consistent with actual tree-ring data. This extremely low CTmin permits Smith fir growing at treeline to complete annual xylem production and maturation and provides both support and a mechanism for treeline formation.

关键词： Cambial activity Critical minimum temperature Timberline Xylem Vaganov-Shashldn model

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