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Functional analysis of the novel mitochondrial tRNA^(Trp)and tRNA^(Ser(AGY))variants associated with type 2 diabetes mellitus

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World Journal of Diabetes 2024年第8期15卷 1753-1763页

作者： yu Ding Xue-jiao yu Qin-Xian Guo Jian-Hang Leng Central Laboratory Hangzhou First People’s HospitalHangzhou 310006Zhejiang ProvinceChina Clinical Laboratory Quzhou People’s HospitalQuzhou 324000Zhejiang ProvinceChina

BACKGROUND Mutations in mitochondrial tRNA(mt-tRNA)genes that result in mitochondrial dysfunction play important roles in type 2 diabetes mellitus(T2DM).We previously reported a large Chinese pedigree with maternally inherited T2DM that harbors novel mt-tRNA^(Trp)A5514G and tRNA^(Ser(AGY))C12237T variants,however,the effects of these mt-tRNA variants on T2DM progression are largely *** To assess the potential pathogenicity of T2DM-associated m.A5514G and m.C12237T variants at genetic,molecular,and biochemical *** Cytoplasmic hybrid(cybrid)cells carrying both m.A5514G and m.C12237T variants,and healthy control cells without these mitochondrial DNA(mtDNA)variants were generated using trans-mitochondrial *** features,including mt-tRNA steady-state level,levels of adenosine triphosphate(ATP),mitochondrial membrane potential(MMP),reactive oxygen species(ROS),mtDNA copy number,nicotinamide adenine dinucleotide(NAD+)/NADH ratio,enzymatic activities of respiratory chain complexes(RCCs),8-hydroxy-deoxyguanine(8-OhdG),malondialdehyde(MDA),and superoxide dismutase(SOD)were examined in cell lines with and without these mt-tRNA *** Compared with control cells,the m.A5514G variant caused an approximately 35%reduction in the steady-state level of mt-tRNA^(Trp)(P<0.0001);however,the m.C12237T variant did not affect the mt-tRNA^(Ser(AGY))steady-state level(P=0.5849).Biochemical analysis revealed that cells with both m.A5514G and m.C12237T variants exhibited more severe mitochondrial dysfunctions and elevated oxidative stress than control cells:ATP,MMP,NAD+/NADH ratio,enzyme activities of RCCs and SOD levels were markedly decreased in mutant cells(P<0.05 for all measures).By contrast,the levels of ROS,8-OhdG and MDA were significantly increased(P<0.05 for all measures),but mtDNA copy number was not affected by m.A5514G and m.C12237T variants(P=0.5942).CONCLUSION The m.A5514G variant impaired mt-tRNA^(Trp)metabolism,which subsequent

关键词： Type 2 diabetes mellitus Mitochondrial tRNA genes Novel variants Oxidative stress Mitochondrial dysfunctions

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Development and validation of a nomogram model for predicting the risk of pre-hospital delay in patients with acute myocardial infarction

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World Journal of Cardiology 2024年第2期16卷 80-91页

作者： jiao-yu Cao Li-Xiang Zhang Xiao-Juan Zhou Department of Cardiology The First Affiliated Hospital of USTCDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina

BACKGROUND Acute myocardial infarction(AMI)is a severe cardiovascular disease caused by the blockage of coronary arteries that leads to ischemic necrosis of the *** medical contact is critical for successful AMI treatment,and delays increase the risk of death for ***-hospital delay time(PDT)is a significant challenge for reducing treatment times,as identifying high-risk patients with AMI remains *** study aims to construct a risk prediction model to identify high-risk patients and develop targeted strategies for effective and prompt care,ultimately reducing PDT and improving treatment *** To construct a nomogram model for forecasting pre-hospital delay(PHD)likelihood in patients with AMI and to assess the precision of the nomogram model in predicting PHD *** A retrospective cohort design was employed to investigate predictive factors for PHD in patients with AMI diagnosed between January 2022 and September *** study included 252 patients,with 180 randomly assigned to the development group and the remaining 72 to the validation group in a 7:3 *** risk factors influencing PHD were identified in the development group,leading to the establishment of a nomogram model for predicting PHD in patients with *** model's predictive performance was evaluated using the receiver operating characteristic curve in both the development and validation *** Independent risk factors for PHD in patients with AMI included living alone,hyperlipidemia,age,diabetes mellitus,and digestive system diseases(P<0.05).A characteristic curve analysis indicated area under the receiver operating characteristic curve values of 0.787(95%confidence interval:0.716–0.858)and 0.770(95%confidence interval:0.660-0.879)in the development and validation groups,respectively,demonstrating the model's good discriminatory *** Hosmer–Lemeshow goodness-of-fit test revealed no statistically significant disparity between the an

关键词： Pre-hospital delay Acute myocardial infarction Risk prediction Nomogram

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Two polyoxovanadoborates with over 100 nuclei assembled from a {V_(14)Co_(12)B_(72)} cluster

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Science China Chemistry 2024年第8期67卷 2578-2585页

作者： Shuang-Xue Wu yu-jiao Dong Yang Yang Chao Qin Chun-Yi Sun Wei Guan Xin-Long Wang Zhong-Min Su Key Laboratory of Polyoxometalate and Reticular Material Chemistry of Ministry of Education Northeast Normal UniversityChangchun 130024China School of Chemistry and Environmental Engineering Changchun University of Science and TechnologyChangchun 130013China Key Laboratory of Advanced Materials of Tropical Island Resources Hainan UniversityHaikou 570228China

Combining theoretical prediction and experimental exploration, the first sandwich-type polyoxovanadoborate(POVB) with an odd number of nuclei, [V_(11)B_(26)O_(73)(OH)_(10)]^(8-)(1), was discovered under hydrothermal conditions. Density-functional theory(DFT)gives a rationalization for the existence of this structure and the structural feature is consistent with the {V_NB_(2N+X)}(X = 2, 4, 6, 8)general formula. Excitingly, when Co and 1,10-phenanthroline(phen) are introduced into the synthesis of vanadoborates system,a 100-nuclei [Co_6(phen)_(10)(H_(2)O)_8][V_(14)Co_(12)B_(72)O_(144)(OH)_(20)]·28H_(2)O(2) and a 102-nuclei [Co_6(phen)_(14)(H_(2)O)_(4)][V_(14)Co_(12)B_(72)O_(144)(OH)_(20)]·23H_(2)O(3) with the same core structure of [V_(14)Co_(12)B_(72)]^(12-)are obtained. The vase-shaped [V_(14)Co_(12)B_(72)] polyanion is a multilayered sandwich-type structure containing seven distinct structural layers and is the largest known POVB. Furthermore, 2demonstrates exceptional thermal and chemical stability and serves as an efficient and stable catalyst for the photoreduction of carbon dioxide.

关键词： boronate metallic materials polyoxovanadoborates density functional theory photocatalysis CO_(2)reduction

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Effectiveness of fecal DNA syndecan-2 methylation testing for detection of colorectal cancer in a high-risk Chinese population

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1361-1373页

作者： Wen-Feng Luo yu-Ting jiao Xiao-Ling Lin Ying Zhao Sheng-Bo Wang Jian Shen Jie Deng yu-Feng Ye Ze-Ping Han Fang-Mei Xie Jin-Hua He yu Wan Central Laboratory of Panyu Central Hospital Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina Digestive Disease Center Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina Shunde Vocational and Technical College Foshan 528300Guangdong ProvinceChina Medical Imaging Institute of Panyu Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina

BACKGROUND Colorectal cancer(CRC)is among the most prevalent and life-threatening malignancies ***-2 methylation(mSDC2)testing has emerged as a widely used biomarker for early detection of CRC in stool and serum ***(CRC)is among the most prevalent and life-threatening malignancies ***2 testing has emerged as a widely used biomarker for early detection of CRC in stool and serum *** To validate the effectiveness of fecal DNA mSDC2 testing in the detection of CRC among a high-risk Chinese population to provide evidence-based data for the development of diagnostic and/or screening guidelines for CRC in *** A high-risk Chinese cohort consisting of 1130 individuals aged 40-79 years was selected for evaluation via fecal mSDC2 *** and specificity for CRC,advanced adenoma(AA)and advanced colorectal neoplasia(ACN)were ***-risk factors for the incidence of colorectal lesions were determined and a logistic regression model was constructed to reflect the efficacy of the *** A total of 1035 high-risk individuals were included in this study according to established *** them,16 suffered from CRC(1.55%),65 from AA(6.28%)and 189 from non-AAs(18.26%);150 patients were diagnosed with polyps(14.49%).Diagnoses were established based upon colonoscopic and pathological *** of the mSDC2 test for CRC and AA were 87.50%and 40.00%,respectively;specificities were 95.61%for other *** predictive values of the mSDC2 test for CRC,AA and ACN were 16.09%,29.89%and 45.98%,respectively;the negative predictive value for CRC was 99.79%.After adjusting for other high-risk covariates,mSDC2 test positivity was found to be a significant risk factor for the occurrence of ACN(P<0.001).CONCLUSION Our findings confirmed that offering fecal mSDC2 testing and colonoscopy in combination for CRC screening is effective for earlier detection of malignant colorectal lesions in a high

关键词： Colorectal cancer Syndecan-2 DNA methylation Chinese population

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Increased Expression of Serum Monocyte Chemoattractant Protein-1,Chemokine(C-C motif)Ligand 3,and Interleukins in Patients With Vitiligo

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International Journal of Dermatology and Venereology 2024年第1期7卷 40-43页

作者： Xiao-Ling Zhao Ya-Lan Li Zi-Han Li Xiao-Chong Zhang Li-Hui An yu-jiao Sun Deng-Xiang Liu Fang Cheng Cancer Laboratory Xingtai People’s HospitalXingtaiHebei 054001China Endocrine Department Xingtai People’s HospitalXingtaiHebei 130500China Dermatological Department Xingtai People’s HospitalXingtaiHebei 054001China

Objective:Vitiligo is a chronic,acquired depigmentation of the skin,which can be divided into active and stable *** stable and active stages are not immutable and often *** of internal markers would help to diagnose the active stage of vitiligo,and the purpose of this study was to find potential active markers in the progression of ***:We compared 30 healthy controls to 60 patients with vitiligo(30 with active disease and 30 with stable disease)regarding the expression of serum monocyte chemoattractant protein(MCP)-1,chemokine(C-C motif)ligand(CCL)3,CCL5,CCL21,interleukin(IL)-6,and *** were collected from May 2017 to December *** detection was carried out by enzyme-linked immunosorbent *** difference between groups was compared by Kruskal-Wallis H test and Mann-Whitney U ***:We found that the serum levels of MCP-1,CCL3,IL-6,and IL-17 were significantly higher in patients with vitiligo than in healthy controls(P<0.01)and that IL-17 was higher in patients with active vitiligo than in those with stable vitiligo(P<0.01).After 3months of treatment,the serum IL-17 level of patients with active vitiligo was not significantly different from that before treatment(P=0.526);after 6months of treatment,however,the serum IL-17 level had significantly decreased(P<0.01).Conclusion:These findings suggest that the serum IL-17 level can be used as a marker of vitiligo disease activity giving it certain guiding significance for clinical treatment.

关键词： clinical marker serum cytokines vitiligo

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Bone Research 2024年第1期12卷 122-136页

作者： Nan-yu Zou Ran Liu Mei Huang yu-Rui jiao Jie Wei Yangzi Jiang Wen-Zhen He Min Huang Yi-Li Xu Ling Liu yu-Chen Sun Mi Yang Qi Guo Yan Huang Tian Su Ye Xiao Wei-Shan Wang Chao Zeng Guang-Hua Lei Xiang-Hang Luo Chang-Jun Li Department of Endocrinology Endocrinology Research CenterXiangya Hospital of Central South UniversityChangshaHunan 410008China Department of Orthopaedics Xiangya Hospital of Central South UniversityChangshaHunan 410008China Department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangshaChina Hunan Key Laboratory of Joint Degeneration and Injury ChangshaHunan 410008China Key Laboratory of Aging-related Bone and Joint Diseases Prevention and Treatment Ministry of EducationXiangya HospitalCentral South UniversityChangshaChina School of Biomedical Sciences Institute for Tissue Engineering and Regenerative MedicineFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Department of Orthopaedics The First Affiliated Hospital of Shihezi UniversityShiheziXinjiangChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Skeletal stem/progenitor cell(SSPC)senescence is a major cause of decreased bone regenerative potential with aging,but the causes of SSPC senescence remain *** this study,we revealed that macrophages in calluses secrete prosenescent factors,including grancalcin(GCA),during aging,which triggers SSPC senescence and impairs fracture *** injection of human rGCA in young mice induced SSPC senescence and delayed fracture *** deletion of Gca in monocytes/macrophages was sufficient to rejuvenate fracture repair in aged mice and alleviate SSPC ***,GCA binds to the plexin-B2 receptor and activates Arg2-mediated mitochondrial dysfunction,resulting in cellular *** of Plxnb2 in SSPCs impaired fracture *** of GCA-neutralizing antibody enhanced fracture healing in aged ***,our study revealed that senescent macrophages within calluses secrete GCA to trigger SSPC secondary senescence,and GCA neutralization represents a promising therapy for nonunion or delayed union in elderly individuals.

关键词： healing union senescence

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优化算法中均值信息利用研究

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东北大学学报（自然科学版） 2024年第1期45卷 49-57页

作者：王方王鹏焦育威中国科学院成都计算机应用研究所四川成都610041 中国科学院大学北京100049 西南民族大学计算机科学与工程学院四川成都610225

研究了启发式优化算法中种群向均值点迁移的策略,并发现该策略对于提升算法性能具有重要影响,同时具备物理和数学含义.通过极大似然估计方法对基态波函数进行参数估计,建立了量子系统达到基态时最优解概率密度函数与种群均值点之间的联... 详细信息

研究了启发式优化算法中种群向均值点迁移的策略,并发现该策略对于提升算法性能具有重要影响,同时具备物理和数学含义.通过极大似然估计方法对基态波函数进行参数估计,建立了量子系统达到基态时最优解概率密度函数与种群均值点之间的联系,并从动力学的角度解释了种群均值点的物理意义.通过在几种经典优化算法上添加利用均值点位置信息的操作,在CEC2013测试集与摄像机布局优化的工程应用上进行对照实验,实验结果表明合理利用均值点位置信息可以有效提升算法的性能.

关键词：量子动力学优化问题均值信息动力学方程极大似然估计

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Study on the expression and prognostic relationship of MYL6B in liver cancer based on bioinformatics

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World Journal of Clinical Oncology 2024年第9期15卷 1188-1197页

作者： Hai-Bing Lv Qing-yun Wu yu-jiao Zhang Sheng-Wei Quan Ning Ma yu-Qing Dai Yan Sun Department of General Surgery Beidahuang Group General HospitalHarbin 150000Heilongjiang ProvinceChina Department of General Surgery Xianning Central HospitalXianning 437000Hubei ProvinceChina Department of Medical oncology Beidahuang Group General HospitalHarbin 150000Heilongjiang ProvinceChina Department of General Surgery Daqing Oilfield General HospitalDaqing 163000Heilongjiang ProvinceChina College of Clinical Medicine Bengbu Medical UniversityBengbu 233000Anhui ProvinceChina Department of General Surgery Second Affiliated Hospital of Harbin Medical UniversityHarbin 150000Heilongjiang ProvinceChina

BACKGROUND Primary liver cancer is a prevalent and deadly cancer *** treatment advances,prognosis remains poor,with high recurrence *** detection is crucial but challenging due to the disease’s insidious *** proteins play significant roles in cancer development,influencing cell migration,invasion,and tumor ***6B,a myosin light chain,is involved in various cellular processes and has been associated with poor prognosis in colorectal adenocarcinoma and potential as a biomarker in breast *** To investigate the expression of MYL6B in liver hepatocellular carcinoma(LIHC)and its impact on prognosis and potential mechanisms of action using bioinformatics *** The expression of MYL6B in pan-cancer and normal tissues was analyzed using the gene expression profiling interactive analysis 2 and tumor immune estimation resource *** expression level of MYL6B in LIHC tissues and its relationship with prognosis were analyzed,immunohistochemical analysis of MYL6B and its effect on immune cell infiltration,and the protein network were further *** MYL6B was highly expressed in diffuse large b-cell lymphoma,LIHC,pancreatic adenocarcinoma,skin cutaneous melanoma,thymoma,uterine corpus endometrial carcinoma,uterine carcinosarcoma,and lowly expressed in kidney chromophobe,acute myeloid leukemia,testicular germ cell *** expression level of MYL6B was significantly different between cancer and normal *** had a significant impact on both overall survival and disease-free ***6B is highly expressed in hepatocellular carcinoma and its expression level increases with cancer *** MYL6B expression is associated with poor prognosis in terms of overall survival and recurrence-free *** immunohistochemical level of MYL6B is high in hepatocellular carcinoma tissues,and MYL6B has a high level of immune infiltration *** protein network analysis,MYL6B is correlated with MYL2

关键词： MYL6B Liver cancer Bioinformatics Prognosis Expression

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超高效液相色谱-四极杆/静电场轨道阱高分辨质谱探究4-HO-MiPT染毒斑马鱼脑部代谢组学变化

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质谱学报 2024年第5期45卷 699-711页

作者：陈宇彬于交远孟梁刘猛赵森浙江警察学院浙江省毒品防控技术研究重点实验室浙江杭州310053 呼伦贝尔市公安局刑事侦查支队内蒙古呼伦贝尔021001 福建警察学院福建福州350007

为评价色胺类新精神活性物质4-羟基-N-甲基-N-异丙基色胺(4-HO-MiPT)对大脑的毒性效应,本实验以斑马鱼为模式生物进行自发活动行为记录分析,采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLCQ/Orbitrap HRMS)分析斑马鱼大脑长... 详细信息

为评价色胺类新精神活性物质4-羟基-N-甲基-N-异丙基色胺(4-HO-MiPT)对大脑的毒性效应,本实验以斑马鱼为模式生物进行自发活动行为记录分析,采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLCQ/Orbitrap HRMS)分析斑马鱼大脑长期染毒后的代谢产物,并在转录水平上检测斑马鱼脑部目标基因的变化。结果表明,注射药物4-HO-MiPT后,斑马鱼运动速度明显减慢,运动范围明显缩小。从斑马鱼大脑检测出37种差异代谢产物,其中7种升高,30种降低,并导致6条代谢通路改变。脑部基因转录水平检测发现,4个神经系统的基因转录水平下降,对大脑细胞产生影响。本实验证明了4-HO-MiPT对斑马鱼大脑产生毒性效应,对神经系统、免疫功能造成损害并影响体内代谢,对斑马鱼行为产生明显的抑制效果,同时从4-HO-MiPT造成的内源性影响推测其可能致癌。

关键词： 4-羟基-N-甲基-N-异丙基色胺(4-HO-MiPT) 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UHPLC-Q/Orbitrap HRMS) 斑马鱼行为模型代谢组学

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黄河内蒙段开河期细菌群落结构特征研究

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中国环境科学 2024年第3期44卷 1487-1496页

作者：史玉娇李文宝郭鑫内蒙古农业大学水资源利用与保护自治区重点实验室内蒙古呼和浩特010018 黄河流域内蒙段水资源与水环境综合治理协同创新中心内蒙古呼和浩特010018

为探索黄河内蒙段开河初期水-土介质中细菌群落组成情况及生态网络结构,于2021年4月中旬进行取样,共获得黄河水样6个和土样4个.利用16S rRNA基因高通量测序方法对水、土样品进行测定,并从菌门及菌属水平对二者的细菌群落组成进行了分析... 详细信息

为探索黄河内蒙段开河初期水-土介质中细菌群落组成情况及生态网络结构,于2021年4月中旬进行取样,共获得黄河水样6个和土样4个.利用16S rRNA基因高通量测序方法对水、土样品进行测定,并从菌门及菌属水平对二者的细菌群落组成进行了分析.结果显示:河漫滩土样细菌群落结构的分布较水样更为分散,且土样中细菌群落丰富度、均匀度及多样性均高于水体,二者多样性存在显著的组间差异;在菌门水平上,变形菌门、放线菌门和酸杆菌门等是构成水-土样品的优势细菌门类,其中变形菌门在河水中相对丰度均值约为36.57%,在土样中则下降到25.39%,而酸杆菌门主要存在土样中,相对丰度约为14.17%;在菌属水平上, hgcI_clade(6.19%~12.75%)、CL500-29_marine_group(2.97%~5.05%)和Acinetobacter(0.74%~12.24%)是水体中的优势菌属,而norank_f__norank_o__norank_c__KD4-96(1.95%~4.91%)、 norank_f__norank_o__Vicinamibacterales(2.35%~4.67%)和norank_f__Gemmatimonadaceae (2.20%~4.73%)是土样中的优势菌属;不同菌属对水-土细菌群落结构的响应并不一致,其中分别归属于酸杆菌门和芽单胞菌门的norank_f__norank_o__Vicinamibacterales及norank_f__Gemmatimonadaceae等是联系水-土细菌群落的主要菌属,而归属于放线菌门的hgcI_clade和CL500-29_marine_group等是造成水、土优势菌门存在差异的主要菌属;虽然土样较水样拥有更大的生态网络结构,且Delftia、Candidatus_Methylopumilus、Arthrobacter和Rb41等是维持黄河内蒙段水、土介质生态网络结构的关键菌属,但相较于土样网络结构,水体网络结构中不同模块关键菌门及菌属间的生态功能较为一致.

关键词：细菌群落水土介质网络结构开河期黄河内蒙段

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