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BIOcELL 2022年第6期46卷 1391-1415页

作者： JUAN cARLOS STOcKERT alfonso blázquez-c.stro Facultad de Medicina Instituto de Oncología“Angel H.Roffo”Universidad de Buenos AiresBuenos AiresC1417DTBArgentina centro Integrativo de Biología y Química Aplicada(cIBQA) Universidad Bernardo O’HigginsSantiago8370854Chile Departamento de Biología Facultad de CienciasUniversidad Autónoma de MadridMadrid28049Spain

Melanins represent one of the most ancient and important group of natural macromolecular *** have multiple biological roles in almost all organisms across the Phyla,examples being photoprotection,anti-oxidative action,radical scavenger activity,and heavy metal *** the biomedical point of view,melanocytes are involved in the origin of melanoma tumors,and the main therapeutic advances for their treatment have been revised in Part 1 of this *** chemical structure of eumelanin is a biological concern of great importance,and therefore,exploring theoretical molecular models and synthesis mechanisms will be here described,as well as molecular orbital features and supramolecular organization,which are responsible for the key properties that make these biological pigments so important,and so ***,this updated overview is devoted to describe present structural models and physico-chemical characteristics of eumelanin,in order to explain and utilize melanin properties on which new photothermal and ultrasonic protocols for melanoma treatment can be devised and applied.

关键词： Eumelanin Melanin models Melanin synthesis Molecular orbitals Supramolecular structure

Biomedical overview of *** melanin biology and chemistry,physico-chemical properties,melanoma tumors,and photothermal therapy

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BIOcELL 2021年第4期45卷 849-862页

作者： alfonso blázquez-c.stro JUAN cARLOS STOcKERT Department of Biology Faculty of SciencesAutonomous University of MadridCantoblancoMadrid28049Spain 2 Centro Integrativo de Biología y Química AplicadaUniversidad Bernardo O’HigginsSantiago8370854Chile 3 Universidad de Buenos AiresFacultad de Ciencias VeterinariasCátedra de Histología y Embriologíae Instituto de Investigación y Tecnología en Reproducción AnimalBuenos AiresC1427CWOArgentina

Melanins(eumelanin,pheomelanin,and allomelanin)represent a very,if not themost,important group of biological *** biological roles are multiple,from photoprotection to antioxidant activity,heavy metal disposal or the myriad uses of color in organisms across all *** the first part of this review,eumelanin biology and some chemical aspects will be presented,as well as key physico-chemical features that make this biological pigment so *** principal characteristics of the melanocyte,the melanin-synthesizing cell in mammals,will also be *** melanocytes are the cause of one of the most devastating known cancers:the malignant *** and molecular signaling aspects will be presented next,as well as the principal advances in promising oncotherapies designed and applied for the treatment of *** particular,on account of the photo-physical properties of melanin,special details will be provided regarding the use of photothermal therapy for melanoma treatment.

关键词： Antitumor therapies Biological pigments Melanin Melanoma Photothermal therapy Skin tumors

In vitro polymerization of the dopamine-borate melanin precursor:A proof-of-concept regarding^(10)boron neutron-capture therapy for melanoma

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BIOcELL 2023年第4期47卷 919-928页

作者： JUAN c.STOcKERT SILVINA A.ROMERO MARcELO N.FELIX-POZZI alfonso blázquez-c.stro Instituto de Oncología“Angel H.Roffo” Área InvestigaciónFacultad de MedicinaUniversidad de Buenos AiresBuenos AiresC1417DTBArgentina centro Integrativo de Biología y Química Aplicada Universidad Bernardo O’HigginsSantiago8370854Chile Departamento de Micro y Nanotecnología Instituto de Nanociencias y NanotecnologíaCentro Atómico ConstituyentesComisión Nacional de Energía AtómicaBuenos AiresCP 1650Argentina Departamento de Biología Universidad Autónoma de MadridMadrid28049Spain

The^(10)boron neutron-capture therapy(BNcT)is an emerging antitumoral method that shows increasing biomedical *** is based on the selective accumulation of the^(10)boron isotope within the tumor,which is then irradiated with low-energy thermal neutrons,generating nuclear fission that produces 7lithium,4helium,andγ*** catechol-borate esters have been rather overlooked as precursors of melanin biosynthesis,and therefore,a proof-of-concept approach for using dopamine-borate(DABO)as a suitable boron-containing candidate for potential BNcT is presented *** can spontaneously oxidize and autopolymerize in vitro,giving a soluble,eumelaninlike brown-black poly-DABO *** melanoma cell cultures treated with 1 mM DABO for 24 and 48 h were viable and showed no signs of damage or cell *** stability and possible trans-esterification of DABO is shortly *** calculations and quantitative structure-activity relationships(QSAR)analysis of DABO and the BNcT agent BPA indicated that they should be cell permeant and accumulate within lysosomes and *** modeling allows visualization of both the DABO precursor and the structure of a borate derivative of the proposed catechol-porphycene model for eumelanin,showing interesting features from molecular orbital *** main difference between DABO and other agents,such as BPA,is that it is not a boronic acid nor a boron *** simple catechol-borate ester(protected from oxidation and blackening)could be administrated to living cells and organisms,in which biosynthesis of boron-melanin in melanoma melanocytes can lead to improved BNcT.

关键词： ^(10)Boron Borate esters catechols Dopamine Eumelanin Melanoma

Entamoeba histolytica acetyl-coA synthetase:biomarker of acute amoebic liver abscess

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Asian Pacific Journal of Tropical Biomedicine 2014年第6期4卷 446-450页

作者： Lim Boon Huat alfonso Olivos Garcia Tan Zi Ning Wong Weng Kin Rahmah Noordin Siti shafiqah Anaqi Azham Lee zhi Jie Guee cher ching Foo Phiaw chong Pim chau Dam Biomedicine Programme.School of Health Sciences Universiti Sains Malaysia Departmento de Medicina Experimental Facultad de MedicinaUniversidad Nacional Autonoma de Mexico Department of Medicine Allianze University College of Medical Sciences Institute for Research in Molecular Medicine 11800 University Sains Malaysia

Objective:To characterize the Entamoeba histolytica(***)antigen(s)recognized by moribound amoebic liver abscess ***:crude soluble antigen of *** was probed with sera of moribund hamsters in 1D-and 2D-Westem blot *** antigenic protein was then sent for tandem mass spectrometry *** corresponding gene was cloned and expressed in Escherichia coli bl21-AI to produce the recombinant *** ADP-forming acetyl-coA synthetase(EhAcS)protein.A customised ELISA was developed to evaluate the sensitivity and specificity of the recombinant ***:A^75 kDa protein band with a pl value of 5.91-6.5 was found to be antigenic;and not detected by sera of hamsters in the control *** mass spectrometry analysis revealed the protein to be the 77 kDa *** ADP-forming acetyl-coA synthetase(EhAcS).The customised ELISA results revealed 100%sensitivity and 100%specificity when tested against infected(n=31)and control group hamsters(n=5)serum samples,***:This rinding suggested the significant role of EhAcS as a biomarker for moribund hamsters with acute amoebic liver abscess(ALA)*** is deemed pertinent that future studies explore the potential roles of EhAcS in better understanding the pathogenesis of ALA;and in the development of vaccine and diagnostic tests to control ALA in human populations.

关键词： Entamoeba histolytica Amoebic liver abscess Biomarker Acetyl-coA synthetase Recombinant EhAcS Western blot