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Computational analyses for cancer biology based on exhaustive experimental backgrounds

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Cancer Drug Resistance 2019年第3期2卷 419-427页

作者： Jun Koseki Masamitsu Konno Hideshi ishii Department of Medical Data Science Osaka UniversityOsaka565-0871Japan Department of Frontier Science for Cancer and Chemotherapy Osaka UniversityOsaka565-0871Japan

Antitumor drug therapy plays a very important role in cancer ***,resistance to chemotherapy is a serious *** studies have been conducted to understand and verify the cause of chemoresistance from multiple points of view such as oncogenes,tumor suppressor genes,DNA mutations and repairs,autophagy,cancer stemness,and mitochondrial metabolism and ***,not only medical data from hospitals but also public big data exist on internet ***,the importance of computational science has vastly increased in biological and medical *** statistical or mathematical analyses of these medical data with conventional experiments,many researchers have recently shown that there is a strong relationship between the biological metabolism and chemoresistance for cancer *** example,folate metabolism that mediates one-carbon metabolism and polyamine metabolism have garnered attention regarding their association with *** has been suggested that these metabolisms may be involved in causing resistance to chemotherapy.

关键词： In silico drug design trans-omics analysis computational analyses cancer biology

Testing the possibility of horizontal transfer of introduced neomycin phosphotransferase(nptII) gene of transgenic Eucalyptus camaldulensis into soil bacteria

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Forestry Studies in China 2008年第2期10卷 134-136页

作者： Katsuaki ishii LU Meng-zhu Forestry and Forest Products Research Institute Tsukuba Norinkenkyu Danchi-nai Ibaraki 305-8687 Japan Research Institute of Forestry Chinese Academy of Forestry Beijing 100091 P. R. China

The possible horizomal transfer of transgenes is of great concern when the transgenic plants are released imo the field. To test the possible transfer of nptII of transgenic trees into soil bacteria, we have used a stool DNA preparation kit to isolate the DNA from the soils in the rhizospheres of two non- and eight transgenic Eucalyptus camaldulensis trees. All the samples have provided the corresponding PCR products in the amplification with bacterial 16S RNA specific sequences, which indicates that the quality of the isolated DNA is adequate for amplification. The nptⅡ specific band has been amplified in three soil samples from the transgenic trees and even treated with filtration before the DNA isolation. This indicates that nptII DNA exists in the soil, although it is still unclear whether the DNA was in the soil particles, in the soil bacteria or in the Agrobacterium comamination which was used for the E. camaldulensis transformation. Two approaches on isolation of bacterial DNA have been suggested for testing the possibility of this event in the future.

关键词： horizontal transfer nptⅡ soil bacteria transgenic trees

Characterization of the effective cellulose degrading strain CTL-6

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Journal of Environmental Sciences 2011年第4期23卷 649-655页

作者： Yucai Lv Xiaofen Wang Ning Li Xiaojuan Wang Masaharu ishii Yasuo Igarashi Zongjun Cui Alan G. MacDiarmid Research Institute of Renewable Energy China Three Gorges University Yichang 443002 China. College of Agronomy and Biotechnology/Center of Biomass Engineering China Agricultural University Beijing 100193 China Graduate School of Agricultural and Life Sciences The University of Tokyo I-I-I Yayoi Bunkyo-Ku Tokyo 113-8657 Japan

An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot be isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain of effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose （80.9% weight loss after 9 days of culture）. The highest efficiency value of the endocellulase （CMCase activity） was 0.404 μmol/（min·mL）, cellulose degradation efficiency by CTL-6 was remarkably high at 50–65°C with the highest degradation efficiency observed at 60°C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so.

关键词： cellulose-degrading bacterium cellulose degradation capability anaerobic bacterium Clostridium

Ultra-thin, planar, Babinet-inverted plasmonic metalenses

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Light(Science & Applications) 2013年第1期2卷 170-175页

作者： Xingjie Ni Satoshi ishii Alexander V Kildishev Vladimir M Shalaev School of Electrical and Computer Engineering and Birck Nanotechnology Center Purdue UniversityWest LafayetteIN 47907USA

We experimentally demonstrate the focusing of visible light with ultra-thin,planar metasurfaces made of concentrically perforated,30-nm-thick gold *** perforated nano-voids—Babinet-inverted(complementary)nano-antennas—create discrete phase shifts and form a desired wavefront of cross-polarized,scattered *** signal-to-noise ratio in our complementary nano-antenna design is at least one order of magnitude higher than in previous metallic nano-antenna *** first study our proof-of-concept‘metalens’with extremely strong focusing ability:focusing at a distance of only 2.5 mm is achieved experimentally with a 4-mm-diameter lens for light at a wavelength of 676 *** then extend our work with one of these‘metalenses’and achieve a wavelength-controllable focal *** characterization of the lens confirms that switching the incident wavelength from 676 to 476 nm changes the focal length from 7 to 10 mm,which opens up new opportunities for tuning and spatially separating light at different wavelengths within small,micrometer-scale *** the proposed designs can be embedded on-chip or at the end of an optical *** designs also all work for two orthogonal,linear polarizations of incident light.

关键词： metalens nano-antennas plasmonics wavefront shaping

Cyclophosphamide-associated enteritis presenting with severe protein-losing enteropathy in granulomatosis with polyangiitis:A case report

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World Journal of Gastroenterology 2021年第20期27卷 2657-2663页

作者： Hiroko Sato Tsuyoshi Shirai Hiroshi Fujii Tomonori ishii Hideo Harigae Department of Hematology and Rheumatology Tohoku University Graduate School of MedicineSendai 9808574Japan

BACKGROUND Although cyclophosphamide(CPA)is the key drug for the treatment of autoimmune diseases including vasculitides,it has some well-known adverse effects,such as myelosuppression,hemorrhagic cystitis,infertility,and ***,CPA-associated severe enteritis is a rare adverse effect,and only one case with a lethal clinical course has been ***,the appropriate management of patients with CPA-associated severe enteritis is *** SUMMARY We present the case of a 61-year-old woman diagnosed with granulomatosis with polyangiitis based on the presence of symptoms in ear,lung,and,kidney with positive myeloperoxidase-antineutrophil cytoplasmic *** received pulsed methylprednisolone followed by prednisolone 55 mg/d and intravenous CPA at a dose of 500 mg/*** days after the second course of intravenous CPA,she developed nausea,vomiting,and diarrhea,and was admitted to the *** testing revealed hypoalbuminemia,suggesting proteinlosing *** tomography revealed wall thickening of the stomach,small intestine,and colon with contrast enhancement on the lumen *** and immunosuppressive therapy were not effective,and the patient’s enteritis did not improve for>4 *** her condition became seriously exhausted,corticosteroids were tapered and supportive therapies including intravenous hyperalimentation,replenishment of albumin and gamma globulin,plasma exchange,and infection control were *** supportive therapies improved her condition,and her enteritis gradually *** was finally discharged 7 mo *** Immediate discontinuation of CPA and intensive supportive therapy are crucial for the survival of patients with CPA-associated severe enteritis.

关键词： Antineutrophil cytoplasmic antibody Cyclophosphamide Enteritis Granulomatosis with polyangiitis Plasma exchange Vasculitis Case report

Coagulation and ablation of biological soft tissue byquantum cascade laser with peak wavelength of 5.7μm

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Journal of Innovative Optical Health Sciences 2014年第3期7卷 115-123页

作者： Keisuke Hashimura Katsunori ishii Naota Akikusa Tadataka Edamura Harumasa Yoshida Kunio Awazu Medical Beam Physics Laboratory Division of Sustainable Energy and Environmental EngineeringGraduate School of Engineering Osaka UiniversityJapan Hamamatsu Photonics K.K.Japan Graduate School of Frontier Biosciences Osaka UniversityJapan The Center for Advanced Medical Engineering and Informatics Osaka University Japan

Molecules such as water,proteins and lipids that are contained in biological tissue absorb mid-infrared(MR)light,which allows such light to be used in laser surgical ***,amides and water exhibit strong absorption bands in the 5-7μm wavelength range,but at present there are no lasers in clinical use that can emit in this ***,the present study focused on thequantum cascade laser(QCL),which is a new type of semiconductor laser that can emit at MIRwavelengths and has recentiy achieved high output power.A high-power QCL with a peakwavelength of 5.7μm was evaluated for use as a laser scalpel for ablating biological soft *** interaction of the laser beam with chicken breast tissue was compared to a conventional CO_(2) laser,based on surface and cross-sectional *** QCL was found to have sufficient power to ablate soft tissue,and its coagulation,carbonization and ablation effects were similar to those forthe CO_(2) *** QCL also induced comparable photothermal effects because it acted as apseudo-continuous wave laser due to its low peak power.A QCL can therefore be used as an effective laser scalpel,and also offers the possibility of less invasive treatment by targeting specificabsorption bands in t he MIR region.

关键词： Quantum cascade laser mid-infrared wavelength CO,laser biological soft tissue laser-tissue interaction.

Development of uncut crop edge detection system based on laser rangefinder for combine harvesters

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International Journal of Agricultural and Biological Engineering 2016年第2期9卷 21-28页

作者： Zhao Teng Noboru Noguchi Yang Liangliang Kazunobu ishii Chen Jun College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100China Vehicle Robotics Laboratory Graduate School of AgricultureHokkaido UniversitySapporoJapan

The objective of this research was to develop an uncut crop edge detection system for a combine harvester.A laser rangefinder(LF)was selected as a primary sensor,combined with a pan-tilt unit(PTU)and an inertial measurement unit(IMU).Three-dimensional field information can be obtained when the PTU rotates the laser rangefinder in the vertical plane.A field profile was modeled by analyzing range ***’s method was used to detect the crop edge position on each scanning profile,and the least squares method was applied to fit the uncut crop *** performance of the system was first evaluated under laboratory ***,validation experiments were conducted under both static and dynamic conditions in a wheat field during harvesting *** verify the error of the detection system,the real position of the edge was measured by GPS for accuracy *** results showed an average lateral error of±12 cm,with a Root-Mean-Square Error(RMSE)of 3.01 cm for the static test,and an average lateral error of±25 cm,with an RMSE of 10.15 cm for the dynamic *** proposed laser rangefinder-based uncut crop edge detection system exhibited a satisfactory performance for edge detection under different conditions in the field,and can provide reliable information for further study.

关键词： laser rangefinder technology crop edge detection combine harvester navigation field profile modeling

Osmotic pressure of serum and cerebrospinal fluid in patients with suspected neurological conditions

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Neural Regeneration Research 2020年第5期15卷 944-947页

作者： Tetsuya Akaishi Toshiyuki Takahashi Ichiro Nakashima Michiaki Abe Masashi Aoki Tadashi ishii Department of Education and Support for Regional Medicine Tohoku University HospitalSendaiJapan Department of Neurology Tohoku University Graduate School of MedicineSendaiJapan Department of Neurology National Hospital Organization Yonezawa National HospitalYonezawaJapan Department of Neurology Tohoku Medical and Pharmaceutical UniversitySendaiJapan

Interstitial fluid movement in the brain parenchyma has been suggested to contribute to sustaining the metabolism in brain parenchyma and maintaining the function of neurons and glial *** pulsatile hydrostatic pressure gradient may be one of the driving forces of this bulk ***,osmotic pressure- related factors have not been studied until *** this prospective observational study,to elucidate the relationship between osmolality (mOsm/kg) in the serum and that in the cerebrospinal fluid (CSF),we simultaneously measured the serum and CSF osmolality of 179 subjects with suspected neurological *** osmolality was 283.6 ± 6.5 mOsm/kg and CSF osmolality was 289.5 ± 6.6 mOsm/*** the specific gravity of serum and CSF is known to be 1.024–1.028 and 1.004–1.007,respectively,the estimated average of osmolarity (mOsm/L) in the serum and CSF covered exactly the same range (i.e.,290.5–291.5 mOsm/L).There was strong correlation between CSF osmolality and serum osmolality,but the difference in osmolality between serum and CSF was not correlated with serum osmolality,serum electrolyte levels,protein levels,or quotient of *** conclusion,CSF osmolarity was suggested to be equal to serum *** is not one of the driving forces of this bulk *** factors such as hydrostatic pressure gradient should be used to explain the mechanism of bulk flow in the brain *** study was approved by the Institutional Review Board of the Tohoku University Hospital (approval *** No.2015-1-257) on July 29,2015.

关键词： brain parenchyma bulk flow cerebrospinal fluid hydrostatic pressure interstitial fluid osmolarity osmotic pressure

Clinical outcomes of endoscopic ultrasonography-guided transmural drainage using plastic stent and nasocystic drain for pancreatic and peripancreatic collections

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Hepatobiliary & Pancreatic Diseases International 2019年第1期18卷 96-99页

作者： Katsuya Kitamura Akira Yamamiya Yu ishii Yuta Mitsui Hitoshi Yoshida Division of Gastroenterology Department of Medicine Showa University School of Medicine

To the Editor:Pancreatic and peripancreatic collections(PCs)develop from acute pancreatitis(AP),chronic pancreatitis,surgery,or *** 2012 revised Atlanta classification[1]of AP classified local complications into the following 4 PC types:acute peripancreatic fluid collection(<4 weeks after the onset of acute interstitial edematous pancreatitis),acute necrotic collection(<4 weeks after the onset of acute necrotizing pancreatitis),pancreatic pseudocyst(PPC;≥4 weeks after the onset of acute interstitial edematous pancreatitis),and walled-off necrosis(WON;≥4 weeks after the onset of acute necrotizing pancreatitis).Endoscopic ultrasonographyguided transmural drainage(EUS-TD)has been reported to be a minimally invasive procedure for patients with PCs[2–4].This study aimed to investigate the clinical outcomes of EUS-TD for PCs.

关键词： peripancreatic EUS-TD