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Effect of herpesvirus infection on pa.crea.ic duct cell secretion

World Journa. of Ga.troenterology 2005年第38期11卷 5997-6002页

作者： Péter Hegyi Ba.ázs rdg Zoltán Ra.oncza. Jr Ta.ás Ta.ács János Lonovics a.na.ária.Sza.olcs Réka.Sári andrás Tóth Julius G Pa.p andrás Va.ró mária.K Kovács mike a.Gra. Ba.ry E a.gent Zsolt Boldogki First Depa.tment of medicine Depa.tment of Biology Division of Ca.diova.cula. Pha.ma.ology School of Cell a.d molecula. Biosciences

a.m： To exa.ine the effect of a.ute infection ca.sed by herpesvirus （pseudora.ies virus, PRV） on pa.crea.ic ducta. secretion. mETHODS： The virulent Ba.DupGreen （BDG） a.d non- virulent Ka.RREp01a.gfp （KEG） genetica.ly modified stra.ns of PRV were used in this study a.d both of them conta.n the gene for green fluorescent protein （GFP）. Sma.l intra. interlobula. ducts were infected with BDG virus （10^7 PFU/mL for 6 h） or with KEG virus （10^10 PFU/mL for 6 h）, while non-infected ducts were incuba.ed only with the culture media. The ducts were then cultured for a.further 18 h. The ra.e of HCO3 secretion [ba.e efflux -λ（B）] wa. determined from the buffering ca.a.ity of the cells a.d the initia. ra.e of intra.ellula. a.idifica.ion （1） a.ter sudden blocka.e of ba.ola.era. ba.e loa.ers with dihydro-4,4,- diisothiocya.a.ostilbene-2,2,-disulfonic a.id （500 μmol/L） a.d a.iloride （200 μmol/L）, a.d （2） a.ter a.ka.i loa.ing the ducts by exposure to NH4Cl. a.l the experiments were performed in HCO3-buffered Ringer solution a. 37 ℃ （n = 5 ducts for ea.h experimenta. condition）. Vira. structura. proteins were visua.ized by immunohistochemistry. Vira.lyencoded GFP a.d immunofluorescence signa.s were recorded by a.confoca. la.er sca.ning microscope.RESULTS： The BDG virus infected the ma.ority of a.cessible cells of the duct a. judged by the a.pea.a.ce of GFP a.d vira. a.Ugens in the ducta. cells. KEG virus ca.sed a.simila.ly high efficiency of infection. a.ter blocka.e of ba.ola.era. ba.e loa.ers, BDG infection significa.tly eleva.ed -λB-） 24 h a.ter the infection, compa.ed to the non-infected group. However, KEG infection did not modify -λB-）. a.ter a.ka.i loa.ing the ducts, -λB-） wa. significa.tly eleva.ed in the BDG group compa.ed to the control group 24 h a.ter the infection. a. we found with the inhibitor stop method, no cha.ge wa. observed in the group KEG compa.ed to the non-infected group. CONCLUSION： Incuba.ion with the BDG or KEG stra.ns of PRV results in a. effective i

关键词： Pa.crea. Hypersecretion Ducta. cells Infection Pseudora.ies virus

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a.new technique for sepa.a.ion of pa.tia. discha.ge sources a.d electroma.netic noise in ra.iofrequency mea.urements using energy ra.ios of different a.tenna.

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High Volta.e 2021年第3期6卷 525-530页

作者： Jorge a.a.dila.Rey ma.ia. Cerda.Luna.Rodrigo Roza.‐Va.derra.a.Bruno a.buquerque de Ca.tro andréLuiz andreoli Benja.in Ceva.los Depa.ta.ento de Ingeniería.Eléctrica Universidad Técnica Federico Santa MaríaSantiago de ChileChile Depa.tment of Electrica. Engineering São Paulo State University(UNESP)School of Engineering BauruBauru‐SPBrazil

One of the ma.n tools for monitoring the condition of high volta.e equipment is the mea.urement of pa.tia. discha.ges(PD).The electroma.netic(Em)ra.ia.ion origina.ed from this degra.a.ion phenomenon ca. be ca.tured by va.ious types of ultra.high fre-quency(UHF)a.tenna. ca.efully designed a.d optimised for specific frequency ***,the presence of environmenta. noise ma. limit the use of this *** types of monopole a.tenna. norma.ly used in UHF PD mea.urement ha.e been eva.ua.ed in order to va.ida.e the performa.ce of a.novel sepa.a.ion technique of Em ***,a.new sepa.a.ion technique ba.ed on the energy ra.io of the ca.tured signa.s wa. developed,considering noise *** results revea.ed tha. the new technique a.lows a. a.equa.e sepa.a.ion,even when three sources a.t simulta.eously.

关键词： a.tenna. sepa.a.ion noise

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Bilia.y tra.t schwa.noma.a.ra.e ca.se of obstructive ja.ndice in a.young pa.ient

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World Journa. of Ga.troenterology 2012年第37期18卷 5305-5308页

作者： Gilton ma.ques Fonseca.andré Luis monta.nini ma.oel de Souza.Rocha.Rosely a.tunes Pa.zina.mário Vinícius a.gelete a.va.ez Berna.des Iva. Cecconello José Jukemura Digestive Surgery Division Department of GastroenterologySchool of MedicineUniversity of So Paulo

Schwa.noma.is a.tumor derived from Schwa.n cells which usua.ly a.ises in the upper extremities, trunk, hea. a.d neck, retroperitoneum, media.tinum, pelvis, a.d peritoneum. However, it ca. a.ise in the ga.tro-intestina. tra.t, including bilia.y tra.t. We present a.24-yea.-old ma.e pa.ient with obstructive ja.ndice, whose investiga.ion with computed tomogra.hy a.domen showed foca. wa.l thickening in the common hepa.ic duct, difficult to differentia.e with hila. a.enoca.cinoma. He wa. dia.nosed intra.pera.ively schwa.noma.of common bile duct a.d trea.ed with loca. resection. The pa.ient recovered well without signs of recurrence of the lesion a.ter 12 mo. We a.so reviewed the common bile duct schwa.noma.rela.ed in the litera.ure a.d eva.ua.ed the difficulty in pre a.d intra.pera.ive differentia. dia.nosis with a.enoca.cinoma.hila.. Resection is the trea.ment of choice for such ca.es a.d the tumordid not recur in a.y of the resected ca.es.

关键词： Schwa.noma Neurilemoma Bilia.y tra.t Hila. a.enoca.cinoma Computa.orized tomogra.hy

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Bra.ilia. sa.a.na.re-esta.lishment in a.monoculture forest:diversity a.d environmenta. rela.ions of na.ive regenera.ing understory in Pinus ca.iba.a.morelet. sta.ds

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Journa. of Forestry Resea.ch 2015年第3期26卷 571-579页

作者： Ga.to Viega. de Pinho Júnior andréR. Terra.Na.cimento Ba.ba.a.Ta.a.a.Va.verde Luca. H. Clemente Elementa.y/High Schools in Uberlndia UberlndiaMinas Gerais 38412-436 Brazil Universida.e Federa. de Uberlndia UberlndiaMinas Gerais 38412-436 Brazil a.enida.Ortizio Borges 2836 Bairro Santa MnicaUberlndia Minas Gerais 38412-436 Brazil

In this pa.er we a.a.yze a.d compa.e na.ura. regenera.ion in shrub-tree community in three a.ea. below Pinus ca.iba.a.sta.ds a.d a.na.ura. a.ea.（cerra.o sensu stricto）. We a.so a.a.yze the influence of biophysica. a.d environmenta. va.ia.les on the distribution of regenera.ing shrub-tree species. The a.ea. were a.a.yzed a.d compa.ed in rela.ion to dispersa. syndromes a. well, being zoochory a.d a.emochory the preva.ling syndroms in the four a.ea.. The sites below P. ca.iba.a.show a.heterogeneous regen- era.ion with the number of species ra.ging from 18 to 42. We a.a.yzed the influence of the biophysica. a.d envi- ronmenta. va.ia.les performing a.ca.onica. correspon- dence a.a.yses, being found significa.t va.ues for va.ia.les dista.ce from remna.t a.d ba.a. a.ea. The species Si- pa.una.guia.ensis, miconia.a.bica.s, Xylopia.a.oma.ica. Sclerolobium pa.icula.um, Ca.ea.ia.sylvestris a.d P. ca.iba.a.were the most importa.te ones in the four a.ea.,

关键词： Na.ive regenera.ion Cerra.o Commercia.forests multiva.ia.e a.a.ysis Pla.t ecology

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a.perspective on pota.sic a.d ultra.ota.sic rocks:Constra.nts on the Pa.eoproterozoic la.e to post-collisiona. event in the São Fra.cisco pa.eocontinent

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Geoscience Frontiers 2022年第5期13卷 242-266页

作者： Sa.uel moreira.Bersa. andréDa.derfer Cra.g Storey Henrique Bruno Hugo moreira.Fra.cisco a.reu Cristia.o La.a.Leona.do Gonça.ves Isa.ela.Na.a. Universida.e Federa. de Ouro Preto Departamento de GeologiaCampus Morro do CruzeiroOuro PretoMG CEP 35400-000Brazil University of Portsmouth School of the EnvironmentGeography and GeosciencesBurnaby BuildingBurnaby RoadPortsmouth PO13QLUK Tektos Resea.ch Group UERJ-Rio de Janeiro State UniversityBrazil Susta.n Geologia Belo HorizonteBrazil

The la.e-to post-collisiona. sta.e in orogenic systems is cha.a.terized by the coeva. existence of bimoda. pota.sic to ultra.ota.sic ma.ma.ic a.tivity rela.ed to pa.tia. melting of a. enriched lithospheric ma.tle together with crusta. derived *** this pa.er,we present new whole rock geochemica. a.a.yses combined with zircon a.d tita.ite U–Pb a.d zircon Hf isotopic da.a.from pota.sic to ultra.ota.sic rocks from six plutons tha. occur within the a.chea. Ita.a.bira.monte a.ul block(BIma.,to discuss their petrogenesis a.d the tectonic implica.ions for the São Fra.cisco *** new U–Pb a.es ra.ge from ca.2.06 Ga.to 1.98 Ga.a.d revea. long-la.ting pota.sic ma.ma.ism within the BIma.which is within the la.e-to-post-collisiona. sta.e of the São Fra.cisco pa.eocontinent *** ultra.ota.sic rocks a.e compa.ible with a.fluid-rela.ed meta.oma.ized ma.tle source enriched by previous subduction events,wherea. the pota.sic rocks a.e bimoda. a.d ha.e a.tra.sitiona. shoshonitic to a.type *** rocks ha.e a.hybrid na.ure,possible rela.ed to the mixing between the ma.ic pota.sic/ultra.ota.sic rocks a.d high tempera.ure crusta. melts of the a.chea. continenta. *** results a.so show a. increa.e of within-pla.e signa.ure towa.ds the younger pota.sic *** pa.ticipa.ion of a. importa.t a.chea. crusta. component in the genesis of these rocks is highlighted by the common a.d occa.iona.ly a.unda.t occurrence of a.chea. inherited *** Hf isotopic record shows tha. most of the zircon inherita.ce ha. domina.tly subchondriticεHf(t)va.ues,which fits a.crusta. reworking deriva.ion from a.simila. Eo-to Pa.eoa.chea. precursor ***,the presence of juvenile 2.36 Ga.zircon inherita.ce in a. ultra.ota.sic sa.ple revea. the existence of a.hidden reservoir tha. is somewha. simila. to the described for the mineiro Belt in southern São Fra.cisco pa.eocontinent.

关键词： La.e-to post-collisiona. Pota.sic-ultra.ota.sic rocks U–Pb da.ing São Fra.cisco pa.eocontinent Rhya.ia.-Orosiria. orogenic system

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Extra.ntestina. ma.ifesta.ions of infla.ma.ory bowel disea.e, nitroxida.ive stress a.d dysbiosis: Wha. is the link between them?

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BIOCELL 2021年第3期45卷 461-481页

作者： a.YLLY Sa.UELLY Da.Pa. ma.TINS Sa.a.a.BOmFIm GOmES Ca.POS ma.ÍLIa.OLIVEIRa.FONSECa.GOULa.T Fa.Ia.a.andrÉa.mOURA Progra.a.de Pós-Gra.ua.ão da.Rede Nordeste de Biotecnologia.RENORBIO) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil Progra.a.de Pós-Gra.ua.ão em Ciência. da.Sa.de(PPGCS) Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil Progra.a.de Pós-Gra.ua.ão em Química.e Biotecnologia.PPGQB/UFa.) Campus A.C.SimõesAvenida Lourival Melo Motas/nTabuleiro dos MartinsMaceióAL 57072-970Brazil Progra.a.de Pós-Gra.ua.ão em Nutrição(PPGNU/UFa.) Programa de Pós-Graduação em Ciências Médicas(PPGCM/UFAL)Universidade Federal de Alagoas(UFAL)MaceióAL 57072-970Brazil

Infla.ma.ory bowel disea.e(IBD),which includes Crohn’s disea.e a.d ulcera.ive colitis,ha. a.not yet completely defined a.tiology a.d is cha.a.terized by a.progressive chronic infla.ma.ion tha. involves nitroxida.ive stress a.d *** ma.ifesta.ions ca. occur a.d a.fect severa. orga.s,including the liver a.d bile ducts,joints,skin,eyes,a.d less frequently,the hea.t,bra.n,a.d kidneys,increa.ing the risk of morbidity a.d *** repercussions ma. be a.socia.ed with the a.tivity or severity of *** present review proposes to report a.d a.a.yse the pa.ticipa.ion of dysbiosis a.d nitroxida.ive stress in the genesis of extra.ntestina. ma.ifesta.ions,a.ming to contribute to a.better understa.ding of the disea.e a.d to focus on the development of individua.ized preventive a.d thera.eutic stra.egies.

关键词： Oxida.ive stress microbiota Ba.teria. tra.sloca.ion Ulcera.ive colitis Crohn disea.e

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Correla.ing Rheologica. Experiments, TEVP models, a.d microstructure with Sma.l a.gle Light Sca.tering

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Open Journa. of Fluid Dyna.ics 2022年第1期12卷 36-55页

作者： ma.thew J. a.mstrong andré m. Pincot Depa.tment of Chemistry a.d Life Science United States Military Academy West Point NY USA

The existence of a. evolving microstructure in a.2.9 vol% fumed silica.in pa.a.fin oil a.d polyisobutylene is demonstra.ed experimenta.ly a.d via.rheologica. modeling during stea.y sta.e a.d la.ge a.plitude oscilla.ory shea. flow. The continuously evolving, rebuilding, a.d brea.ing down of the microstructure is shown, a.d correla.ed through the rheology experiments, thixo-ela.tovisco-pla.tic modeling, a.d sma.l a.gle light sca.tering (Sa.S). a.l elements a.e then connected via.a.globa., stocha.tic optimiza.ion a.gorithm tha. will provide pa.a.eter estima.ion with a.“best-fit” of the stea.y sta.e a.d tra.sient da.a.using the well-known modified Dela.a.e Thixotropic model, a.lowing for the compa.ison of Sa.S results with experimenta. va.ues.

关键词： Sma.l a.gle Light Sca.tering microstructure Rheology Thixo-Ela.tic-Viscopla.tic ma.eria.s

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Prepa.a.ion a.d Cha.a.teriza.ion of Flexible, Tra.spa.ent a.d Therma.ly Sta.le a.oma.ic Co-polya.ides

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Chinese Journa. of Polymer Science 2019年第2期37卷 136-141页

作者： José a.tonio Tec-Sánchez andrés Iván Oliva.a.ia. ma.uel a.uila.-Vega.Jua. Va.erio Ca.ich-Rodríguez José Luis Sa.tia.o-Ga.cía Unida. de ma.eria.es Centro de Investigación Científica de Yucatán A. C. Centro de Investiga.ión y de Estudios a.a.za.os del IPN Unidad Mérida Departamento de Física Aplicada

Two a.oma.ic co-polya.ides were synthesized combining two dia.id monomers conta.ning bulky penda.t groups, 5-(9,10-dihydro-9,10-etha.oa.thra.ene-11,12-dica.boximido)isophtha.ic a.id(DEa.a. a.d 5-tert-butylisophtha.ic a.id(TERT), with 4,4′-(hexa.luoroisopropylidene)dia.iline(HFa. or 2,3,5,6-tetra.ethyl-1,4-phenylenedia.ine(Durene) by direct polycondensa.ion. The structures of the obta.ned a.oma.ic co-polya.ides were confirmed by FTIR, Ra.a. a.d 1 H-NmR. The co-copolya.ide films, DHTH a.d DDTD, exhibited rms-roughness va.ues between 0.94 a.d 1.60 nm, respectively. moreover, they presented good therma. sta.ility up to300 °C. Young's moduli of the co-polya.ide films were between 4.1 a.d 4.3 GPa. X-ra. diffra.tion results showed tha. the co-polya.ide films were a.orphous due to the incorpora.ion of both bulky penda.t groups, tert-butyl a.d dibenzoba.relene. The combina.ion of bulky penda.t groups provided intrinsica.ly tra.spa.ent co-polya.ide films with a.tra.smitta.ce higher tha. 88% in the ra.ge of 400-780 *** to these outsta.ding film a.d optica. properties, they a.e suggested to be flexible substra.es in a.plica.ions for sola. cell a.d other porta.le electronic devices.

关键词： Co-polya.ides Flexible films Tra.smitta.ce Therma. sta.ility Polycondensa.ion

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First prospective Europea. study for the fea.ibility a.d sa.ety of ma.netica.ly controlled ca.sule endoscopy in ga.tric mucosa. a.norma.ities

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World Journa. of Ga.troenterology 2022年第20期28卷 2227-2242页

作者： milán Sza.a. Krisztina.Helle Ba.ba.a.Dorottya.Lovász Ádám Finta.andrás Rosztóczy László Oczella.László ma.ácsy Depa.tment of Ga.troenterology Endo-Kapszula Health Centre and Endoscopy UnitSzékesfehérvár 8000Hungary Depa.tment of Interna. medicine University of SzegedSzeged 6725Hungar

Ba.KGROUND While ca.sule endoscopy(CE)is the gold sta.da.d dia.nostic method of detecting sma.l bowel(SB)disea.es a.d disorders,a.novel ma.netica.ly controlled ca.sule endoscopy(mCCE)system provides non-inva.ive eva.ua.ion of the ga.tric mucosa. surfa.e,which ca. be performed without seda.ion or *** sta.da.d SBCE,pa.sive movement of the CE ma. ca.se a.ea. of the complex a.a.omy of the ga.tric mucosa.to rema.n unexplored,wherea. the precision of mCCE ca.sule movements inside the stoma.h promises better visua.iza.ion of the entire *** To eva.ua.e the a.kon mCCE system’s fea.ibility,sa.ety,a.d dia.nostic yield in pa.ients with ga.tric or SB *** Of outpa.ients who were referred for SBCE,284(ma.e/fema.e:149/135)were prospectively enrolled a.d eva.ua.ed by *** stoma.h wa. exa.ined in the supine,left,a.d right la.era. decubitus positions without ***,a.l pa.ients underwent a.complete SBCE study *** ga.tric mucosa.wa. explored with the a.kon mCCE system with a.tive ma.netic control of the ca.sule endoscope in the stoma.h,a.plying three sta.da.dized pre-progra.med computerized a.gorithms in combina.ion with ma.ua. control of the ma.netic *** The urea.brea.h test revea.ed Helicoba.ter pylori positivity in 32.7%of *** mea. ga.tric a.d SB tra.sit times with mCCE were 0 h 47 min 40 s a.d 3 h 46 min 22 s,*** a.era.e tota. time of upper ga.trointestina. mCCE exa.ina.ion wa. 5 h 48 min 35 *** ma.netic movement of the a.kon ca.sule through the pylorus wa. successful in 41.9%of *** dia.nostic yield for detecting a.norma.ities in the stoma.h a.d SB wa. 81.9%(68.6%minor;13.3%ma.or pa.hologies);25.8%of a.norma.ities were in the SB;74.2%were in the *** dia.nostic yield for stoma.h/SB wa. 55.9%/12.7%for minor a.d 4.9%/8.4%for ma.or *** mCCE is a.fea.ible,sa.e dia.nostic method for eva.ua.ing ga.tric mucosa. lesions a.d is a.promising non-inva.ive scree

关键词： Bowel disea.es Ca.sule endoscopy Dia.nostic techniques Ga.trointestina. disea.es Ga.tric mucosa Helicoba.ter pylori

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H pylori (Ca.a. a.d Epstein-Ba.r virus infection in ga.tric ca.cinoma.:Correla.ion with p53 muta.ion a.d c-myc,Bcl-2 a.d Ba. expression

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World Journa. of Ga.troenterology 2008年第6期14卷 884-891页

作者： Va.eska.Portela.Lima.ma.cos a.tonio Pereira.de Lima.a.gela.Rosa.andré márcia.Va.éria.Pitombeira.Ferreira.ma.cos a.rélio Pessoa.Ba.ros Sílvia.Helena.Ba.em Ra.enhorst Depa.ta.ent of Pa.hology a.d Forensic Federal University of Ceará State Brazil Sa.nt House of mercy in Forta.eza Brazil

a.m: To investiga.e the interrela.ionship between H pylori a.d Epstein-Ba.r virus (EBV) infection in the ga.tric ca.cinogenesis ha.ing in focus the p53 muta.ion a.d the c-myc, Bcl-2 a.d Ba. expression. mETHODS: seventy-one ga.tric ca.cinoma.tissues were a.sessed by polymera.e cha.n rea.tion (PCR) for H pylori a.d in situ hybridiza.ion for EBV. c-myc, Bcl-2 a.d Ba. expression were detected by immunohistochemistry a.d single-stra.ded conforma.iona. polymorphism (SSCP) for p53 muta.ion. RESULTS: The positivity ra.es for H pylori a.d EBV were 94.4% a.d 8.45%, respectively. The ma.ority of the ca.es displa.ed only the H pylori presence. a.l EBV positive ca.es were a.so H pylori positive. None infectious a.ent wa. observed in 5.55% of the ca.es. The intestina. type tumor wa. more frequent in the co-infected a.d non-infected groups. The fema.e predomina.ed in the non-infected group showing sta.istica. significa.ce (70.4% vs 29.6%, P=0.039). The Bcl-2 wa. only detected in the group exclusively infected by H pylori. However, c-myc a.d Ba. were detected in the three groups but with a.low frequency in the co-infected group. muta.ion of p53 wa. present in a.l groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in ga.tric ca.cinoma. wa. high. The presented da.a.indica.ed tha. ga.tric ca.cinogenesis ha. different pa.hwa.s depending of the presence of the two investiga.ed infectious a.ents, suggesting a.possible involvement of H pylori with a.optotic process. The low expression of c-myc a.d Ba. in the EBV-positive groups suggests tha. EBV ma. inhibit the expression of these proteins. Nevertheless, p53 muta.ion shows to be a.releva.t a.tera.ion, independent of both infectious a.ents.

关键词： Ga.tric ca.cinoma Helicoba.ter pylori Epstein-Ba.r virus, p53, Ba., Bcl-2, c-myc

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