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prp mutants with different numbers of octarepeat sequences are more susceptible to the oxidative stress

Science china(Life Sciences) 2008年第7期51卷 630-639页

作者： AN run1 ,2,DONG ChenFang 2,LEI YanJun 1,2,HAN Lu 2,LI ping 2,CHEN JianMing 2,WANG Guirong 2, SHI Qi 2 ,GAO Chen 2 ,JIANG HuiYing 2 ,ZHOU Wei 2 ,HAN Jun 2 ,CHU YongLie 1 ,DONG Xiaoping 2 1School of Medicine,Xi’an JiaoTong University,Xi’an 710061,china 2State Key Laboratory for Infectious disease prevention and control,National Institute for Viral disease control and prevention, Chinese center for disease control and prevention,YingXin rd 100,Beijing 100052,china 1. School of Medicine Xi’an JiaoTong University Xi’an 710061 China 2. State Key Laboratory for Infectious disease prevention and control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention YingXin Rd 100 Beijing 100052 China

One of the physiological functions of cellular prion protein(prp C )is believed to work as a cellular resistance to oxidative stress,in which the octarepeats region within prp plays an important ***,the detailed mechanism is less *** this study,the expressing plasmids of wild-type prp (prp-pG5)and various prp mutants containing 0(prp-pG0),9(prp-pG9)and 12(prp-pG12)octarepeats were generated and prp proteins were expressed both in *** and in mammalian *** aggregation and formation of carbonyl groups were clearly seen in the recombinant prps expressed from *** after treatment of *** and trypan blue staining assays revealed that the cells expressing the mutated prps within octarepeats are less viable than the cells expressing wild-type *** significant high levels of intracellular free radicals and low levels of glutathione peroxidase were observed in the cells transfected with plasmids containing deleted or inserted *** more productions of carbonyl groups were detected in the cells expressing prps with deleted and inserted octarepeats after exposing to ***,cells expressing wild-type prp showed stronger resistant activity to the challenge of H2O2 at certain extent than the mutated prps and mock. These data provided the evidences that the octarepeats number within prp is critical for maintaining its activity of *** of its protective function against oxidative stress may be one of the possible pathways for the mutated prps to involve in the pathogenesis of familial Creutzfeldt-Jacob diseases.

关键词： prion protein oxidative stress glutathione peroxidase reactive oxygen species cell viability

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新城疫病毒分子生物学及其疫苗的研究进展

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草业与畜牧 2011年第9期 7-10页

作者：侯巍四川省动物疫病预防控制中心四川成都610041

新城疫是当今全球范围内最严重的家禽传染病之一,特别是对养鸡业造成重大经济损失。利用分子生物技术已研究出了很多基因工程疫苗用于预防鸡新城疫,比如基因工程活载体疫苗、亚单位疫苗和DNA疫苗等。近年来随着反向遗传操作技术的发展,... 详细信息

新城疫是当今全球范围内最严重的家禽传染病之一,特别是对养鸡业造成重大经济损失。利用分子生物技术已研究出了很多基因工程疫苗用于预防鸡新城疫,比如基因工程活载体疫苗、亚单位疫苗和DNA疫苗等。近年来随着反向遗传操作技术的发展,新城疫病毒已经被开发成病毒载体,可以携带外源基因研制新城疫病毒活载体疫苗,表达IBDV和IBV的新城疫活载体疫苗已经成功研制。本文将从新城疫病毒的分子生物学及疫苗方面进行综述。

关键词：新城疫病毒分子生物学疫苗

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Application of 16S rDNA real-time Fluorescent Quantitate pCr for Detecting Gamma Germ

Application of 16S rDNA Real-time Fluorescent Quantitate PCR...

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第三届第八次全国学术研讨会暨动物微生态企业发展战略论坛

作者： HUANG Yongcheng,CHENG Anchun,WANG Mingshu,YANG Xiaoyan,QI Xuefeng, GE Zhongyuan,GUO Yufei,ZHANG Suhui,HUji,CHEN Xiaoyue (Avian disease research center, College of animal Science and Veterinary Medicine of sichuan Agricultural University Key Laboratory of animal disease and Human Health of sichuan province, Yaan, sichuan, china,625014)

Group-specificity quantitate pCr primers and probe were designed and synthesized ,and method of detecting Bacillus was *** optimal Mg2+ concentration of the real-time fluorescent quantitate pCr was 7.5 mmol/L, and the optimal probe concertration was 0.1 μmol/L;The detecting of Bacillus pumilus with the method was masculine, but to Bifidobacterium bifidus,***, Lactobacillus bulgaricus, Enterococcus faecium,and Staphylococcus aureus those were existed in animal intestinal tract was negative;the lowest detect examine was 8 copies of 16S rDNA;Extracted DNA of the duodenal, jejunal, ileac, cecal ,and rectal contents of ducks at 20,21, 22,23,26,32,41 ,and 56d of age was detected with this method , results indicated these intestinal tract spots has the bacillus to exist, the quantity of Bacillus in different intestinal tract spot has not obvious difference. This method has high sensitivity and specificity, which could detect fast the quantity of Bacillus in the duck intestinal tract.

关键词： Bacillus 16S rDNA real-time fluorescent quantitate pCr

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Analysis of causes for liver function deteriora-tion in patients with HIV/HCV co-infection

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Hepatobiliary & pancreatic diseases International 2004年第4期3卷 538-542页

作者： Yong-Hong Zhang, Xin-Yue Chen, Yan Jiang, Yao Xiao, Zhen Liu, Xiao-Shan Long and Hao Wu Beijing, china Department of Infectious diseases, Beijing You’an Hospital and china center of disease of control and prevention , Beijing 100054, china Department of Infectious diseases You'an Hospital Beijing 100054 China.

BACKGrOUND:Co-infection of hepatitis C virus (HCV) and human immunodeficiency virus type 1 ( HIV-1 ) is common in hemophiliacs and drug abusers. To assess the interaction between HIV and HCV disease progression, we examined 82 HIV/HCV co-infection patients and 62 HCV infection patients. METHODS: Liver function, pathological changes, infec- tion duration, immune function and qualitative HCV-rNA and HCV antibody were compared retrospectively between the two groups of patients. rESULTS: Fourty-eight patients (58.5%) in the HIV/ HCV co-infection group and 53 patients (85.5%) in the HCV infection group showed abnormal liver function. No significant difference was observed in inflammation and fi- brosis in the two groups p =0.187, 0.954). However, liver abnormality in the patients with HIV/HCV co-infection appeared 8 years earlier than in those with HCV infection alone (p8+ T in the HIV/HCV group were (226.35 ± 173.49)×106/L and (914. 40 ±448. 28)×106/L, whereas in the HCV group they were (752.31±251.69)×l06/L and (529.011170.67)×106/L respectively. The difference in the two groups was highly significant (ppratio of the number of people with both HCV-rNA and HCV antibody positive to the number of HCV-rNA positive and HCV antibody negative in the HIV/HCV group was 52:9, whereas in the HCV group it was 44:1 (p = 0.043). CONCLUSION: HIV/HCV co-infection can accelerate de- terioration of hepatitis C, which may be due to the effect of HIV on cellular immunity and humoral immunity of the body.

关键词： HIV virus hepatitis C virus CD4+T cell HCV-rNA

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Effects of Zearalenone on IL-2,IL-6,and IFN-γ mrNA levels in the splenic lymphocytes of chickens

Effects of Zearalenone on IL-2,IL-6,and IFN-γ mRNA levels i...

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中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Y.C.Wang~1,J.L.Deng~(1,*),S.W.Xu~(2*),X.peng~3,Z.C.Zuo~4,H. M.Cui~3,Y.Wang~4,Z.H.ren~4 (1 College of Veterinary Medicine,sichuan Agricultural University,Yaan,sichuan 625014,p.r. china,2 Department of Veterinary Medicine,Northeast Agricultural University,Harbin 150030, p.r.china,3sichuan Key Laboratory of animal disease and Human Health,Yaan,sichuan, 625014,p.r.china sichuan 625014,p.r.china,4 sichuan Key Laboratory of environmental hazards disease,Yaan,sichuan 625014,p.r.china)

Zearalenone(ZEN) is an estrogenic mycotoxin produced by several Fusarium species,which can contaminate food and *** compounds elicit a wide spectrum of toxic effects,including the capacity to alter normal immune *** this study,the in vitro effects of the treatment of ConA-stimulated splenic lymphocytes with ZEN(0-25μg/mL) were *** modulates the expression of IL-2,IL-6,and IFN-γ. The IL-2 levels were up to four fold higher(ppared with the levels in the control levels at toxin concentrations of 25μg/mL after 48 h of *** IL-6 levels were critically suppressed at this concentration;these changes were very statistically significant(pr ZEN concentrations(0.***.0.4 and 1.6μg/mL), the IFN-γlevels changed slightly;however at 6.25 and 25μg/mL,the IFN-γresults reached statistical significance compared with the control levels(ppotent effects on the expression of chicken splenic lymphocytes cytokines at the mrNA level.

关键词： zearalenone mycotoxin immunity chicken cytokines

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百日咳防控方案(2024年版)(节选)

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国际流行病学传染病学杂志 2024年第4期51卷 217-220页

作者：国家疾病预防控制局国家卫生健康委员会不详

百日咳(pertussis)是由百日咳鲍特菌(Bordetella pertussis)感染引起的急性呼吸道传染病,是《中华人民共和国传染病防治法》规定的乙类传染病。为进一步科学精准做好百日咳防控工作,及时发现并有效处置百日咳疫情,切实维护人民群众生命... 详细信息

百日咳(pertussis)是由百日咳鲍特菌(Bordetella pertussis)感染引起的急性呼吸道传染病,是《中华人民共和国传染病防治法》规定的乙类传染病。为进一步科学精准做好百日咳防控工作,及时发现并有效处置百日咳疫情,切实维护人民群众生命安全和身体健康,制定本方案。

关键词：百日咳预防控制

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Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue

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Science china(Life Sciences) 2009年第3期52卷 284-295页

作者： LI Mei & ZHAO ChunJiang College of animal Science and Technology,china Agricultural University,Beijing 100193,china 1. College of animal Science and Technology China Agricultural University Beijing 100193 China

Oxygen concentration is essential for appropriate *** can exert a significant impact on physiological alteration of the cell and *** Chicken(Gallus gallus) is a Chinese indigenous breed inhabiting in Tibetan areas,which is also a chicken breed living at high altitude for the longest time in the *** has developed an adaptive mechanism to hypoxia,which is demonstrated by that Tibetan Chicken has much higher hatchability than low-land chicken breeds in high-altitude areas of *** the present study,Tibetan Chicken fertilized full sib eggs were incubated up to Hamburger-Hamilton stage 43 under 13% and 21% oxygen concentration,*** Chicken and Dwarf recessive White Chicken were used as control *** hearts in all of the 3 chicken breeds under hypoxic and normoxic conditions were isolated and hybridized to Genechip Chicken Genome Array to study molecular mechanisms underlying the adaptation to high altitude of Tibetan *** a result,50 transcripts highly expressed in hypoxia are screened *** up-regulated genes,some are involved in the gene ontology(GO) such as cell growth,cell difference,muscle contraction and signal ***,the expression levels of 21 transcripts are lower in hypoxia than those in *** down-regulated genes take part in cell communication,ion transport,protein amino acid phosphorylation and signal ***,gene enrichment analyses of these differential gene expressions are mainly associated with immune system response and ion channel activity in response to ***,the transcriptional expression profiles analyzed by hierarchical clustering and Cpp-SOM software in all of the 3 different chicken breeds revealed that Tibetan Chicken is much closely related to Shouguang Chicken rather than Dwarf recessive White *** addition,12 transcripts of Tibetan Chicken breed-specific expressed genes were identified,which seem to result in a more

关键词： adaptation chronic hypoxia gene chip chicken genome array Tibetan Chicken(Gallus gallus) differential gene expression

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传染病疫情应急预案管理办法

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国际流行病学传染病学杂志 2024年第3期51卷 145-147页

作者：国家疾病预防控制局国家卫生健康委员会不详

第一条为构建分级分类、高效实用的传染病疫情应急预案体系, 规范传染病疫情应急预案管理工作, 增强传染病疫情应急预案的专业性、针对性和实用性, 依据《中华人民共和国突发事件应对法》《中华人民共和国传染病防治法》《突发公共卫... 详细信息

第一条为构建分级分类、高效实用的传染病疫情应急预案体系, 规范传染病疫情应急预案管理工作, 增强传染病疫情应急预案的专业性、针对性和实用性, 依据《中华人民共和国突发事件应对法》《中华人民共和国传染病防治法》《突发公共卫生事件应急条例》《突发事件应急预案管理办法》《国家疾病预防控制局职能配置、内设机构和人员编制规定》等法律法规和工作制度, 制定本办法。

关键词：传染病暴发应急管理

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Studies on adaptation of new type gosling viral enteritis virus in duck embryo fibroblasts

Studies on adaptation of new type gosling viral enteritis vi...

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The 15th World Veterinary poultry Congress

作者： Shun Chen Anchun Cheng Ming-shu Wang Avian disease research center Key Laboratory of sichuan provincial animal disease and Human Health College of animal Science and Veterinary Medicine of sichuan Agricultural University

＜正＞The essential prerequisite for the experimental investigation of a virus it causes is the production in some laboratory host because virus can’t replicate or multiply without any host *** far,there is a lack of...

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ESTABLISHMENT OF IMMUNOGLOBULIN M(IgM)-IMMUNOSOrBENT AGGLUTINATION ASSAY(ISAGA) FOr DIAGNOSIS OF TOXOpLASMOSIS

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中国寄生虫学与寄生虫病杂志 1999年第4期 35-37页

作者： ZHANG Shuyi, WEI Meixiong, ZHAO Huifen, SHI Gongfen Shanghai Detecting center for Toxoplasmosis Shanghai Institute of parasitic disease control and rsesarch,Shanghai 200336

AIM∶To establish an immunosorbent agglutination assay (ISAGA)for detection of IgM antibodies against Toxoplasma gondii. METHODS∶In the ISAGA,wells of microtiter plates were coated with anti\|human IgM antibodies and sealed with 1% bovine serum *** the test sera were added and incubated,the plates were washed, *** tachyzoite antigen suspension was added, and incubated overnight at 37℃. The ISAGA results were evaluated by comparing with those detected by Danish ISAGA and ELISA and those detected by slide enzyme immunoassay (S\|EIA). rESULTS∶ Forty\|four sera from Danish pregnant women were tested by the IgM\|ISAGA,41(93\^2%)were consistent with the Danish results. Sixty\|seven sera from Danish and Shanghai pregnant women were detected by IgM\|IgM\|ISAGA and S\|EIA, the total consistency rate was 92\^5%.A significant correlation was found between the titers of the ISAGA and S\|EIA,with a pearson correlation coefficient of 0\^589( p rs of ISAGA were eighteen times higher than those of S\|*** method enables the detection of IgM antibodies as low as approximately 0\^08 IU/***∶The IgM\|ISAGA is therefore sensitive,specific, easy to perform, and is useful for mass screening and diagnosing recent Toxoplasma infection or reactivation.

关键词： Toxoplasma gondii immunodiagnosis IgM\|ISAGA

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