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Quantitative detection of ING1 mRNA under different gene regulation based on molecular beacon

Chinese Science Bulletin 2006年第17期51卷 2059-2064页

作者： LIU Bin1,2,3,4, WANG Kemin1,2,3,4, XIAO Zhiqiang5.6, WANG Wei1,3,4, TAN Weihong1,2,3,4, SUN Yi5.6, TANG Hongxing1,3,4 & YANG Xiaohai1,3,41. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hu- nan university, changsha 410082, china 2. Life Science and Biotechnology Institute, Hunan university, Chang- sha 410082, china 3. Chemistry and Chemical Engineer College, Hunan university, changsha 410082, china 4. Hunan Engineering research center for Bio-Nanotechnology, Hunan university, changsha 410082, china 5. medical experiment research center, xiangya hospital,{15., changsha 4.0008, china 6. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, changsha 4.0008, china State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha China Life Science and Biotechnology Institute Hunan University Changsha China Chemistry and Chemical Engineer College Hunan University Changsha China Hunan Engineering research center for Bio-Nanotechnology Hunan University Changsha China medical experiment research center Xiangya Hospital Central South University Changsha China Key Laboratory of Cancer Proteomics of Chinese Ministry of Health Changsha China

The effects of drug treatment, gene transfection, and p5. RNA interference (RNAi) on level of ING1 mRNA in tumor cells were quantitatively detected with the help of ING1 molecular beacon (MB)/cRNA standard curve based on MB detection technology. Results showed that the level of ING1 mRNA could be upregulated by 5.FU treatment or ING1 transfection in low expression cell line of MCF-7 and be inhibited by silencing p5. with RNAi technology in normal expression cell line of CNE2. The level of ING1 varied from 7.7×10?16 to 5..4× 10?16 mol/μg total RNA of tumor cells. The results not only provided evidence for the regulation effects of gene expression but also were applied to investigat- ing the interaction of multigenes on signal transduc- tion pathway.

关键词： ING1 RT-PCR 定量分析分子信号 mRNA

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Multiple dose pharmacokinetics of risperidone and 9-hydroxyrisperidone in Chinese female patients with schizophrenia

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Acta Pharmacologica Sinica 2006年第3期27卷 381-386页

作者： Zhi-ling ZHOU~2 Xin LI~3 Huai-yan PENG~4 Xi-yong YU~2 Ming YANG~2 Feng-li SU~1 Feng WANG~1 Rong-hua ZHU~1 Chun-yu DENG~2 Qiu-xiong LIN~2 Chuan-yue WANG~5.Wen-biao LI~5.Shu-guang LIN~2 Huan-de LI~(1,6) ~1Clinical Pharmacy research Institute,xiangya Second hospital,central south {5.,changsha 410011,china ~2research center of medical Science,Guangdong Provincial People's hospital,Guangzhou 5.0080,china ~3Vascular Surgery Department,xiangya Second hospital,central south {5.,changsha 410011,china ~4Department of Clinical Laboratory,Third xiangya hospital,central south {5.,changsha 410013,china ~5.ffiliated Beijing An Ding hospital,Capital {5. of medical Science,Beijing 100088,china ClinicalPharmacyresearchInstitute XiangyaSecondHospitalCentralSouthUniversityChangsha410011China researchcenteroJmedicalScience GuangdongProvincialPeople'sHospitalGuangzhou510080China

Aim:To study the multiple dose clinical pharmacokinetics of risperidone and itsmain active metabolite,9-hydroxyrisperidone,in Chinese female patients ***:The subjects were 23 Chinese female inpatients aged18-65.years who met the CCMD-Ⅲ（third revision of the Chinese Criteria of Men-tal Disorders）criteria for *** were tested after 17 d of treat-ment with 2 mg risperidone twice *** concentrations of risperidone and9-hydroxy-risperidone were assayed by using validated high performance liquidchromatography-mass spectrometry（HPLC-MS）***:Risperidonewas rapidly absorbed(Tmaxwas 1.6 h)and its T1/2in plasma was short（3.2 h）.9-hydroxy-risperidone was quickly metabolized from the parent drug with a meanTmaxof 2.5.*** had a long half-life of 24.7 *** Cavssof risperidone and 9-hydroxy-risperidone were 36.9±33.1 and 110.6±30.5.μg·h·L-1,respectively,and the AUC0-12sswere 443.2+397.4 and 1327.2±402.3 μg·h·L-1,***/F and V/F ofrisperidone were 8.7±6.2 L/h and 34.1±24.3 L,*** varia-tions for pharmacokinetic parameters were quite large for *** 23 sub-jects experienced high prolactin levels when treated with *** was no correlation between prolactin level and the concentration ofrisperidone,9-hydroxy-risperidone,or the active ***:Risperidoneshowed large interindividual variations in *** ofrisperidone resulted in high serum prolactin *** results indicate that sys-temic exposure to risperidone and 9-hydroxy-risperidone in female Chinese schizo-phrenic patients is higher relative to published data for white Caucasian *** studies regarding the PK/PD relationship may be required to develop areasonable clinical dosage regimen for Chinese female patients.

关键词： pharmacokinetics risperidone 9-hydroxyrisperidone schizophrenia

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Novel ATP7B mutations and found effects in Chinese Han families with Wilson's disease

Novel ATP7B mutations and found effects in Chinese Han famil...

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中国的遗传学研究——遗传学进步推动中国西部经济与社会发展——2011年中国遗传学会大会

作者： Hua-rong Yang~(1,2),Shao-juan Gu~(1,2),Yong Qi~1,Xiong Deng~1,Jing Lei~(1,2),Jing Li~3,Le Zhang~3,Hui Liang~1,Yi Guo~4,Xiao-liu Shi~5.Hao Deng~(1,2,*) 1 center for experimental Medicine,the Third xiangya hospital,central south {5.,changsha,china 2 Department of Neurology,the Third xiangya hospital,central south {5.,changsha,china 3 Department of Neurology,xiangya {15.,central south {5.,changsha,china 4 Department of Physiology,xiangya medical School,central south {5.,changsha,china 5.Department of Gastroenterology,the Second xiangya hospital,central south {5.,changsha,china

Wilson’s disease(WD) is an autosomal recessive disorder of copper metabolism and it is caused by mutations in the ATP7B *** investigate the ATP7B mutation in nine Chinese Han families with WD,direct genomic DNA sequencing was performed to identify the ATP7B *** different mutations,including 4 novel mutations(Vall45.he,Gln388X, Thr498Ser and Gly837X) were identified in all 9 Chinese Han *** patients harbor homozygous or compound heterozygous ATP7B mutations,whereas heterozygous ATP7B mutation carriers were all free of any clinical *** analysis of different families with the same mutations revealed that founder effects for Arg778Leu,Pro992Leu and Ala1295.al mutations exist in these *** results may provide new insights into the cause and diagnosis of WD.

关键词： Wilson’s disease the mutation the ATP7B gene haplotype analysis

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Coenzyme Q10 protects human dopaminergic cell line SHSY-5. against rotenone-induced apoptosis

Coenzyme Q10 protects human dopaminergic cell line SHSY-5Y a...

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中国药理学会临床药理学专业委员会会议暨第十次全国临床药理学学术会议

作者： DENG Sheng~1 ZHANG Wei~2 YIN Tao~1 TANG Ling~1 DENG Hao~3 1 Department of Pharmacy,xiangya hospital,central south university,{5. 4.0008,Hunan,china 2 pharmacogenetics research Institute,Institute of Clinical Pharmacology,central south university,{5. 410078,Hunan,china 3 medical {15.al center,The Third xiangya hospital,central south university,{5. 410013,Hunan,china

AIM:To determine whether the use of an antioxidant re- agent coenzyme Q10 can protect human dopaminergic cell line SHSY-5. against rotenone-induced apoptosis. METHODS:Cell viability was quantified by 3-(4,5.dim- ethyhhiazol-2-yl)-2,5.diphenyhetrazolium bromide(MTF) assay and the apoptosis induced by rotenone was observed by Hoechst 33342,propidium iodide,and ealcein AM staining in SHSY-5. cells. RESULTS:Rotenone,a commonly used natural pesti- cide,inhibitor of mitochondrial respiratory chain complex I,was shown to decrease cell viability and induce apopto- sis in SHSY-5. *** with coenzyme Q10,the electron transporter in the mitochondrial respiratory chain, remarkably increased cell viability as well as significantly reduced the percentage of apoptotic SHSY5. cells induced by rotenone. CONCLUSION:Coenzyme QI0 has a beneficial effect in protecting against rotenone-indueed apoptosis in SHSY-SY *** results support the hypothesis that coenzyme QI0 is a component of the antioxidant machinery that pro- tects cell membranes from oxidative damage and decreases *** evaluation is required to determine whether the neuroprotective action of coenzyme Q10 can be used for the prevention and therapy of neurodegenerative diseases.

关键词： Coenzyme Q10 rotenone cell viability apoptosis

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Association between eIF3α Polymorphism and Severe Toxicity Caused by Platinum-based Chemotherapy in Non-Small Cell Lung Cancer Patients

Association between eIF3α Polymorphism and Severe Toxicity ...

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传承与发展，创湖南省生理科学事业的新高——湖南省生理科学会2011年度学术年会

作者： Xiaojing Xu1,Lifang Han1,Li Duan2,Yingchun Zhao3,Huaping Yang4,Boting Zhou5.Honghao Zhou1,Zhaoqian Liu1(1Institute of Clinical Pharmacology,Hunan Key Laboratory of Pharmacogenetics,central south university,changsha,Hunan 410078,china 2 Department of Oncology,Zhongnan {5.,Wuhan university,Wuhan,Hubei 430071,china 3 Osteoporosis research center,Creighton university medical center,Omaha,NE 68124,USA 4 Department of Respiratory Medicine,xiangya {5.,central south university,changsha,Hunan 4.0008,china 5.Department of Pharmacy,xiangya {5.,central south university,Hunan 4.0008,china)

Background Platinum-induced toxicity severely impedes successful chemotherapy in lung cancer *** excision repair(NER)pathway is considered as one of the major factors contributing to platinum side effects. Furthermore... 详细信息

关键词： eIF3α,non-small cell lung cancer(NSCLC),platinum toxicity,ototoxicity,nephrotoxicity,survival analysis

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Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

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Cell research 2003年第4期13卷 251-263页

作者： YING CHEN, ZHI Xu HE, AILIAN LIU, KAI WANG, WEN WEI MAO, JIAN XIN CHU, YONG LU, ZHENG Fu FANG, YING TANG SHI, QING ZHANG YANG, DA YUAN CHEN, MIN RANG WANG, JIN SONG LI, SHAO LIANG HUANG, XIANG YIN KONG, YAO ZHOU SHI, Zm QIANG WANG, JIA HuI XIA, ZHI GAO LONG, ZHI GANG XUE, WEN XIANG DING, HuI ZHEN SHENG center for Developmental Biology, Shanghai Second medical university, Shanghai 200092, china Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 1665.Kong Jiang Road, Shanghai 200092, china center for Stem Cell research, the Second Affiliated hospital, Sun Yat-Sen university, Guangzhou 5.0120,china State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 19 Zhong-guan Cun Road, Haidian District, Beijing 100080, china Health Science center, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences and Shanghai Second medical university, Shanghai 200025. china National Laboratory of medical Genetics, central south university, 88 xiangya Road, changsha 410078, china Xinhua hospital, Shanghai Second medical university, 1665.Kong Jiang Road, Shanghai 200092, china 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 3center for Stem Cell research the Second Affiliated Hospital Sun Yat-Sen University Guangzhou 510120 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 2Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 1665 Kong Jiang Road Shanghai 200092 China. 4State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences 19 Zhong-guan Cun Road Haidian District Beijing 10008

To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5. 42, 5. and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well

关键词： nuclear transfer (NT) somatic cell nuclear transfer (SCNT) embryonic stem cells (ES cell) therapeutic cloning rabbit oocyte.

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工作记忆负荷对注意捕获的影响研究

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心理科学 2011年第1期34卷 33-37页

作者：张斌张智君蔡太生中南大学湘雅二医院医学心理研究所长沙410011 浙江大学心理与行为科学系杭州310028

注意捕获研究对于探讨两种控制过程:自上而下的加工与自下而上的加工之间的关系具有重要的意义。本文通过检验不同工作记忆负荷对注意捕获的影响,探讨自上而下的认知控制对注意捕获的影响机制。本研究共包括二个实验:实验一比较了单、... 详细信息

注意捕获研究对于探讨两种控制过程:自上而下的加工与自下而上的加工之间的关系具有重要的意义。本文通过检验不同工作记忆负荷对注意捕获的影响,探讨自上而下的认知控制对注意捕获的影响机制。本研究共包括二个实验:实验一比较了单、双任务条件对颜色干扰子注意捕获的影响;实验二探讨了不同工作记忆负荷对颜色干扰子注意捕获的影响。实验结果表明:工作记忆不仅在选择性注意任务中具有认知控制作用,而且这种作用能扩展到注意捕获现象中。注意捕获不是纯粹的刺激驱动的加工过程,它还受到自上而下的认知控制的调节。具体表现为,随着工作记忆负荷水平的增加,无关干扰子产生的注意捕获的程度增大。

关键词：注意捕获认知控制工作记忆

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The G/A Allele of eIF3a rs37405.6 Predicts Platinum-based Chemotherapy Resistance in Lung Cancer Patients

The G/A Allele of eIF3a rs3740556 Predicts Platinum-based Ch...

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传承与发展，创湖南省生理科学事业的新高——湖南省生理科学会2011年度学术年会

作者： Xiaojing Xu1,Lifang Han1,Ruixia Yuan2,Huaping Yang3,Boting Zhou1,4,Yingchun Zhao5.Honghao Zhou1,Zhaoqian Liu1(1Institute of Clinical Pharmacology,Hunan Key Laboratory of Pharmacogenetics,central south university,changsha,Hunan Province,410078,china 2 Department of Oncology,Zhongnan {5.,Wuhan university,Wuhan,Hubei Province,430071,china 3 Department of Respiratory Medicine,xiangya {5.,central south university,changsha,Hunan Province,410078,china 4Department of Pharmacy,xiangya{5.,central south university,Hunan 410078,china 5.Osteoporosis research center,medicalcenter of Creighton university,Nebraska68131,USA)

Purpose The purpose of this study was to investigate possible mutations in promoter and exon regions of eIF3a gene and to assess whether eIF3a mutation status have prognostic and predictive significance in platinum-ba... 详细信息

关键词： eIF3a,lung cancer,platinum chemotherapy,resistance,survival analysis

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鼻咽癌细胞中p5.相互作用蛋白质的分离和鉴定

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生物化学与生物物理进展 2004年第7期31卷 628-634页

作者：胡巍肖志强陈主初李建玲张鹏飞冯雪萍易红余艳辉唐新科刘清萍梁宋平中南大学湘雅医院医学实验研究中心中南大学湘雅医学院肿瘤研究所长沙410078 湖南师范大学生命科学学院长沙410081

鼻咽癌中p5.3基因突变罕见 ,但绝大部分鼻咽癌中存在p5.3蛋白过表达 /聚集且功能失活 .然而 ,到目前为止p5.3蛋白失活的机制仍然不清楚 .为揭示鼻咽癌中p5.3蛋白功能失活的机制 ,采用免疫共沉淀技术分别富集鼻咽癌细胞系HNE1和HNE2的p5.... 详细信息

鼻咽癌中p5.3基因突变罕见 ,但绝大部分鼻咽癌中存在p5.3蛋白过表达 /聚集且功能失活 .然而 ,到目前为止p5.3蛋白失活的机制仍然不清楚 .为揭示鼻咽癌中p5.3蛋白功能失活的机制 ,采用免疫共沉淀技术分别富集鼻咽癌细胞系HNE1和HNE2的p5.3结合蛋白 ,SDS 聚丙烯酰胺凝胶电泳 (SDS PAGE)对免疫沉淀复合物进行分离 ,从胶中切取p5.3结合蛋白条带 ,胶内酶解后进行电喷雾串联质谱 (LC ESI MS/MS)分析 ,得到相应的肽序列标签 (peptidesequencetags ,PST) ,通过搜索数据库在鼻咽癌细胞系中鉴定了 9个p5.3结合蛋白 .分别是热休克蛋白 70 (HSP70 )家族成员GRP 78和GRP 75.HSP90家族成员GRP 94、核纤层蛋白A/C (LaminA/C)、α actinin 4、Ezrin/Cytovillin、DNA复制准许因子 /MCM3蛋白 (DNAreplicationlicensingfactor/minichromosomemaintenance 3protein ,MCM3)、CD98/ 4F2heavychain和蛋白激酶C (PKC) .并用免疫共沉淀和蛋白质印迹分析技术对HNE1细胞蛋白条带 3鉴定的p5.3相互作用蛋白之一HSP78进行了验证 .首次在鼻咽癌细胞中鉴定了 9个p5.3结合蛋白 ,为阐明鼻咽癌中p5.3蛋白聚集及失活的机制提供了重要依据和线索 .

关键词： p5. 鼻咽癌免疫共沉淀液相色谱-电喷雾串联质谱相互作用蛋白蛋白质印迹

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Immunization with chlamydial plasmid protein pORF5.DNA vaccine induces protective immunity against genital chlamydial infection in mice

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Science china(Life Sciences) 2008年第11期51卷 973-980页

作者： LI ZhongYu1,2, WANG ShiPing1, Wu YiMou2, ZHONG GuangMing3 & CHEN Ding3 1 xiangya School of Medicine, central south university, changsha 410078, china 2 Department of Microbiology and Immunology, university of {5. china, Hengyang 421001, china 3 university of Texas Health Science center at San Antonio, TX 78229, USA xiangya School of Medicine Central South University Changsha 410078 Chin

To validate the immune protective efficacy of pORF5.DNA vaccine and to analyze potential mechanisms related to this protection. In this study, pORF5.DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial infection. Groups of BALB/c mice were immunized intranasally with pORF5.DNA vaccine. Humoral and cell mediated immune responses were evaluated. The clearance ability of chlamydial challenge from the genital tract and the chlamy- dia-induced upper genital tract gross pathology and histopathological characterization were also de- tected. The results showed that the total and the IgG2a anti-pORF5.antibody levels in serum were sig- nificantly elevated after pcDNA3.1-pORF5.vaccination, as were the total antibody and IgA levels in vaginal fluids. pcDNA3.1-pORF5.induced a significantly high level of Th1 response as measured by robust gamma interferon (IFN-γ). Minimal IL-4 was produced by immune T cells in response to the re-stimulation with pORF5.protein or the inactive elementary body in vitro. pcDNA3.1-pORF5.vacci- nated mice displayed significantly reduced bacterial shedding upon a chlamydial challenge and an accelerated resolution of infection. 100% of pcDNA3.1-pORF5.vaccinated mice successfully resolved the infection by day 24. pcDNA3.1-pORF5.immunized mice also exhibited protection against patho- logical consequences of chlamydial infection. The stimulated index was significantly higher than that of mice immunized with pcDNA3.1 and PBS (P5.. Together, these results demonstrated that immu- nization with pORF5.DNA vaccine is a promising approach for eliciting a protective immunity against a genital chlamydial challenge.

关键词： Chlamydia trachomatis pORF5 DNA vaccine immune protection Th1 immune response

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