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Establishment of bovine prion peptide-based monoclonal antibodies for identifying bovine prion

science china(Life sciences) 2009年第8期52卷 754-760页

作者： ZHAO Li1,HOU XinSheng5,JI Rong2,HAN ChunHui2,YU XiuPing3,HONG Tao4,5 1 Department of Laboratory science of Microbiology,school of Public Health,Shandong university,Jinan 250012,china 2 institute of Nutrition and Food Safety,Chinese Center of Disease Prevention and Control,beijing 100021,china 3 Department of Microbiology,school of Medicine,Shandong university,Jinan 250012,china 4 institute of Virus Disease Prevention and Control,Chinese Center for Disease Control and Prevention,beijing 100052,china 5 institute of biological science and technology,school of science,beijing jiaotong university,beijing 100044,china Department of Laboratory science of Microbiology School of Public Health Shandong University Jinan 250012 China institute of Nutrition and Food Safety Chinese Center of Disease Prevention and Control Beijing 100021 China Department of Microbiology School of Medicine Shandong University Jinan 250012 China institute of Virus Disease Prevention and Control Chinese Center for Disease Control and Prevention Beijing 100052 China institute of biological science and technology School of Science Beijing Jiaotong University Beijing 100044 China

To obtain high titer monoclonal antibodies(McAbs) which can react with mammalian prion protein(PrP),Balb/C mice were immunized with bovine(Bo) PrP peptide(BoPrP 209-228 aa) coupled to keyhole limpet hemocyanin(KLH).The hybridoma cell lines secreting monoclonal antibodies against the peptide were established by cell fusion and *** obtained McAbs were applied to detect recombinant human,bovine and hamster PrP,cellular prion protein(PrPc) in normal bovine brain and pathogenic scrapie prion protein(PrPSc) accumulated in the medulla oblongata of bovine spongiform encephalopathy(BSE)specimen with Western blot and immunohistochemical detection,*** current procedure might offer a simple,feasible method to raise high titer antibodies for studying biological features of PrP in mammals,as well as detection of transmissible spongiform encephalopathy(TSE) and diagnosis of BSE,in particular.

关键词： prion monoclonal antibody Western blot immunohistochemistry ELISA

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Stem cells and neurodegenerative diseases

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science china(Life sciences) 2008年第4期51卷 287-294页

作者： HOU LingLing & HONG Tao institute of biological science and technology, beijing jiaotong university, beijing 100044, china 1. institute of biological science and technology Beijing Jiaotong University Beijing 100044 China

Neurodegenerative diseases are characterized by the neurodegenerative changes or apoptosis of neurons involved in networks, which are important to specific physiological functions. With the de-velopment of old-aging society, the incidence of neurodegenerative diseases is on the increase. How-ever, it is difficult to diagnose for most of neurodegenerative diseases. At present, there are too few effective therapies. Advances in stem cell biology have raised the hope and possibility for the therapy of neurodegenerative diseases. Recently, stem cells have been widely attempted to treat neurodegen-erative diseases of animal model. Here we review the progress and prospects of various stem cells, including embryonic stem cells, mesenchymal stem cell and neural stem cells and so on, for the treatments of neurodegenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, Hunt-ington’s disease and Amyotrophic lateral sclerosis/Lou Gehrig’s disease.

关键词： stem cells neurodegenerative diseases therapy

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An efficient algorithm for solving supply chain network equilibria and equivalent supernetwork based traffic network equilibria

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science china(Technological sciences) 2010年第12期53卷 3264-3274页

作者： XU Meng1 & GAO ZiYou2 1 MOE Key Laboratory for Urban Transportation Complex Systems Theory and technology, beijing jiaotong university, beijing 100044, china 2 institute of Systems science, school of Traffic and Transportation, beijing jiaotong university, beijing 100044, china 1. MOE Key Laboratory for Urban Transportation Complex Systems Theory and technology Beijing Jiaotong University Beijing 100044 China2. Institute of Systems Science School of Traffic and Transportation Beijing Jiaotong University Beijing 100044 China

This paper is concerned with the algorithm of the supply chain network equilibrium model and its equivalent supernetwork based traffic network equilibrium model with elastic demands. Both models are further written as nonlinear complementarity problems. Semismooth least squares reformulations of the complementarity problems are presented and their convergence properties are investigated. Considering the drawbacks of Quasi-Newton method (using the Fischer-Burmeister function), a semi-smooth Levenberg-Marquardt-type method is proposed to solve the problems. Numerical examples show that the Levenberg-Marquardt-type method can solve the supply chain network equilibrium model and its equivalent supernetwork based traffic network equilibrium model significantly, and is more efficient than the Quasi Newton method and the modified projection method. Furthermore, the Levenberg-Marquardt-type method with the equivalent supernetwork based complementarity formulation can be implemented faster than with the supply chain network equilibrium complementarity formulation.

关键词： supply chain networks traffic network equilibrium supernetwork nonlinear complementarity problem Levenberg Marquardt-type method

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A new method for counting trees with vertex partition

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science china Mathematics 2008年第11期51卷 2000-2004页

作者： LIU YanPei institute of Mathematics, beijing jiaotong university, beijing 100044, china institute of Mathematics Beijing Jiaotong University Beijing 100044 China

A direct and elementary method is provided in this paper for counting trees with vertex partition instead of recursion, generating function, functional equation, Lagrange inversion, and matrix methods used before.

关键词： tree planted tree joint tree model vertex partition code 05C30 05C10

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RST transforms resistant image watermarking based on centroid and sector-shaped partition

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science china(Information sciences) 2012年第3期55卷 650-662页

作者： ZHAO Yao1,2*, NI RongRong1,2* & ZHU ZhenFeng1,2* 1institute of Information science, beijing jiaotong university, beijing 100044, china 2beijing Key Laboratory of Advanced Information science and Network technology, beijing 100044, china 1. institute of Information science Beijing Jiaotong University Beijing 100044 China2. Beijing Key Laboratory of Advanced Information Science and Network Technology Beijing 100044 China

A novel digital watermarking scheme featuring centroid-based sectoring is proposed in this paper. To get higher robustness against geometric attacks, such as rotation, scaling, and translation (RST), a delicate synchronization mechanism was developed and incorporated into the proposed approach. During the process of watermark embedding, the original image was partitioned into sectors based on the image centroid. Synchronization information as well as the message bits is then embedded into these sectors. With the help of the centroid-based sectoring and synchronization information, the proposed approach is capable of restoring the correct sectoring even if it has experienced severe geometric distortion. This attribute ensures the correct recovery of embedded watermarks and contributes to the robustness of the proposed scheme. A series of experiments have been conducted to verify the feasibility and effectiveness of the proposed approach. Experimental results show that the proposed scheme possesses good robustness against RST attacks and considerable robustness against other common image processing attacks.

关键词： digital watermarking geometrical transform attacks RST transforms

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Determination of the packing number D_λ(3,W_4^((3)),v)

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science china Mathematics 2009年第11期52卷 2537-2548页

作者： WU Yan & CHANG YanXun institute of Mathematics,beijing jiaotong university,beijing 100044,china 1. institute of Mathematics Beijing Jiaotong University Beijing 100044 China

A t-hyperwheel(t≥3) of length l(or W l(t) for brevity) is a t-uniform hypergraph(V,E) ,where E = {e1,e2,. . .,el} and v1,v2,. . .,vl are distinct vertices of V = il=1 ei such that for i = 1,. . .,l,vi,vi+1 ∈ ei and ei ∩ ej = P,j ∈/{i-1,i,i + 1},where the operation on the subscripts is modulo l and P is a vertex of V which is different from vi,1 i l. In this paper,we investigate the maximum packing problem of MPλ(3,W 4( 3) ,v) . Finally,the packing number Dλ(3,W 4( 3) ,v) is determined for any positive integers v 5 and λ.

关键词： t-(v Γ λ) packing leave packing number group divisible(Γ t) -design candelabra(Γ t) -system

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Performances of carbon nanotube field effect transistors with altered channel length

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science china(Technological sciences) 2010年第1期53卷 253-256页

作者： FENG Ying,HUANG ShiHua,KANG Kai & FENG YuGuang institute of Optoelectronic technology,beijing jiaotong university,beijing 100044,china 1. institute of Optoelectronic technology Beijing Jiaotong University Beijing 100044 China

The influence of channel length on the performances of carbon nanotube field effect transistors(CNT-FETs) has been *** oxide etching was used to remove approximately a 60 nm layer from the original 100 nm silicon dioxide layer,to thin the dielectric layer of the back *** length of the CNT-FETs was changed along with the etching *** dependence of drain-source current on gate voltage was measured to analyze the performance of the CNT-FETs,including the transconductance,carrier mobility,current ON/OFF ratio,*** results indicate that the devices still keep good quality.

关键词： carbon nanotube field effect transistors mobility buffered oxide etching

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The spectrum of （gv, g, 3, λ）-DF in Zgv

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science china Mathematics 2009年第5期52卷 1004-1016页

作者： WANG XiaoMiao & CHANG YanXun institute of Mathematics, beijing jiaotong university, beijing 100044, china 1. institute of Mathematics Beijing Jiaotong University Beijing 100044 China

In this paper, the necessary and sufficient conditions for the existence of a (gv, g, 3, λ)- difference family in Zgv are established. As a consequence, the existence spectrum of a cyclic (3, λ)-GDD of type gv witho... 详细信息

关键词： difference family incomplete difference matrix perfect difference family

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Stability Test for 2-D Continuous-Discrete Systems

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Journal of Systems Engineering and Electronics 2002年第2期13卷 22-27页

作者： Xiao Yanginstitute of Information science, Northern jiaotong university, beijing 100044, P.R. china(Received July 25, 2001) institute of Information science Northern Jiaotong University Beijing 100044 P. R. China

Models of 2-D continuous-discree system are introduced, which can be used to describe some complex systems. Different from classical 2-D continuous systems of 2-D discrete systems, the asymptotic stability of the continuous-discrete systems is determined by Hurwitz-Schur stability (hybrid one) of 2-D characteristic polynomials of the systems. An algebraic algorithm with simpler test procedure for Hurwitz-Schur stability test of 2-D polynomials is developed. An example to illustrate the applications of the test approach is provided.

关键词： Asymptotic stability Polynomials Two dimensional

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Bursting of Morris-Lecar neuronal model with current-feedback control

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science china(Technological sciences) 2009年第3期52卷 771-781页

作者： DUAN LiXia1, LU QiShao2 & CHENG DaiZhan3 1 College of science, North china university of technology, beijing 100144, china 2 school of science, beijing university of Aeronautics and Astronautics, beijing 100191, china 3 institute of Systems science, Chinese Academy of sciences, beijing 100190, china College of science North China University of Technology Beijing 100144 China school of science Beijing University of Aeronautics and Astronautics Beijing 100191 China institute of Systems science Chinese Academy of Sciences Beijing 100190 China

The Morris-Lecar (ML) neuronal model with current-feedback control is considered as a typical fast-slow dynamical system to study the combined influences of the reversal potential VCa of Ca2+ and the feedback current I on the generation and transition of different bursting oscillations. Two-parameter bifurcation analysis of the fast subsystem is performed in the parameter (I, VCa)-plane at first. Three important codimension-2 bifurcation points and some codimension-1 bifurcation curves are obtained which enable one to determine the parameter regions for different types of bursting. Next, we further divide the control parameter (V0, VCa)-plane into five different bursting regions, namely, the "fold/fold" bursting region R1, the "fold/Hopf" bursting region R2, the "fold/homoclinic" bursting region R3, the "subHopf/homoclinic" bursting region R4 and the "subHopf/subHopf" bursting region R5, as well as a silence region R6. Codimension-1 and -2 bifurcations are responsible for explanation of transition mechanisms between different types of bursting. The results are instructive for further understanding the dynamical behavior and mechanisms of complex firing activities and information processing in biological nervous systems.

关键词： neuron bursting bifurcation fast-slow dynamics analysis codimension

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