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Urotensin Ⅱ increases endothelin production by vascular smooth muscle cells in rats

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Chinese Science Bulletin 2002年第12期47卷 1007-1010页

作者： Ql Yongfen, BU Dingfang, YANG Jun, ZHANG Zhaokang, NIU Dadi, SHI Yanrong, ZHANG Zhenghao, TANG Chaoshu & DU JunbaoInstitute of Cardiovascular Diseases, The First Hospital of peking university, beijing 100083, China department of {3., health Science Institute of Cardiovascular Diseases First Hospital of Peking University Beijing 100083 China

The aim of this study was to observe the effects of urotensin Ⅱ (UII) on production of endothelin (ET) in rat aortic vascular smooth muscle cells (VSMC). Cultured VSMCs incubated with various concentrations of UII were used to measure the YSMC 3.-TdR incorporation, the amount of ET mRNA and ET production in VSMCs. In this work we found that UII (10-10-10-8 mol/L) promoted VSMC 3.-TdR incorporation (47%-83., P5) to 112.8% (P 5.3. and 7.12 pg/mL (P 3. a specific ETA receptor antagonist, obviously decreased the VSMC DNA synthesis induced by

关键词： urotensin II endothelin mRNA peptide/interaction.

Carbon isotope ratios of C_(4) plants in loess areas of North China

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Science China Earth sciences 2006年第1期49卷 97-102页

作者： WANG Guoan1,2,3. HAN Jiamao 2, ZHOU Liping3. XIONG Xiaogang4, TAN Ming2, WU Zhenhai5 & PENG Jun6 1. department of Plant Nutrition, China Agricultural university, beijing 100094, China 2. Institute of Geology and Geophysics, Chinese Academy of {3., beijing 100029, China 3. College of Environmental {3., MOE Laboratory for Earth Surface Processes, peking university, beijing 100871, China 4. Institute of Botany, Chinese Academy of {3., beijing 100093. China 5. Northwest Sci-Tech university of Agriculture and Forestry, Yangling 712100, China 6. Network center, China Agricultural university, beijing 100094, China 1. department of Plant Nutrition China Agricultural University Beijing 100094 China 2. Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China 3. College of Environmental sciences MOE Laboratory for Earth Surface Processes Peking University Beijing 100871 China 4. Institute of Botany Chinese Academy of Sciences Beijing 100093 China 5. Northwest Sci-Tech university of Agriculture and Forestry Yangling 712100 China 6. Network center China Agricultural University Beijing 100094 China

Carbon isotope ratios (δ^(13.C) of 89 C_4 plant samples were determined fromthe loess area in North China. δ^(13.C values vary between -10.5per thousand and -14.6per thousandwith a mean of -12.6per thousand. Along a precipitation gradient from the semi-moist area to thesemiarid area, then to the arid area, the δ^(13.C values of C_4 plants show a slight decreasingtrend. The δ^(13.C values of C_4 plants in the dry season are found lower than those in the wetseason. These trends are opposite to those observed for C_3.species.

关键词： C4 plants, carbon isotope ratios, loess area.

Interannual variability of Antarctic Oscillation and its influence on East Asian climate during boreal winter and spring

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Science China Earth sciences 2006年第5期49卷 554-560页

作者： FAN Ke1,2,3.& WANG Huijun2 1. Nansen-Zhu International Research center, Institute of Atmospheric Physics, Chinese Academy of sciences, beijing 100029, China 2. {3. of Atmospheric Science, Yunnan university, Kunming 650091, China 3. Graduate university of the Chinese Academy of sciences, beijing 100049, China 1. Nansen-Zhu International Research center Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China 2. department of Atmospheric Science Yunnan University Kunming 650091 China 3. Graduate university of the Chinese Academy of sciences Beijing 100049 China

The interannual variability of Antarctic Oscillation (AAO) and its influence on East Asian climate during both boreal winter and spring are addressed. The results show that the positive AAO anomaly decreases the cold activity over East Asia during both boreal winter and spring. AAO-related barotropic meridional teleconnection from Antarctic to Arctic is found through analysis of mean me- ridional circulations. This meridional teleconnection is remarkable over Eurasia during boreal winter and over the Pacific Ocean during boreal spring. The results also show that zonal mean zonal wind at high latitudes in Southern Hemisphere has well positive correlation with that of Eurasia during boreal winter and has negative correlation with Pacific North American teleconnection (PNA) during boreal spring, which again display the meridional teleconnection. Thus, local meridional teleconnection is a possible linkage for interaction of circulations at mid-high latitudes between both hemispheres.

关键词： Antarctic Oscillation, Eurasian west wind, meridional teleconnection, mean meridional circulation.

Landscape ecology：the theoretical foundation of sustainable agrolandscape planning and design

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Journal of Environmental sciences 1995年第3期7卷 289-296页

作者： Wang Yanglin(department of Geography, peking university, beijing 100871 , China)Fu Bojie(Research {3. for Eco-Environrnental Sceiences, Chinese Acaderny of sciences, beijing 100085, China)

Ｌａｎｄｓｃａｐｅｅｃｏｌｏｇｙ：ｔｈｅｔｈｅｏｒｅｔｉｃａｌｆｏｕｎｄａｔｉｏｎｏｆｓｕｓｔａｉｎａｂｌｅａｇｒｏｌａｎｄｓｃａｐｅｐｌａｎｎｉｎｇａｎｄｄｅｓｉｇｎ．ＷａｎｇＹａｎｇｌｉｎ（Ｄｅｐａｒｔｍｅｎｔｏ... 详细信息

Ｌａｎｄｓｃａｐｅｅｃｏｌｏｇｙ：ｔｈｅｔｈｅｏｒｅｔｉｃａｌｆｏｕｎｄａｔｉｏｎｏｆｓｕｓｔａｉｎａｂｌｅａｇｒｏｌａｎｄｓｃａｐｅｐｌａｎｎｉｎｇａｎｄｄｅｓｉｇｎ．ＷａｎｇＹａｎｇｌｉｎ（ＤｅｐａｒｔｍｅｎｔｏｆＧｅｏｇｒａｐｈｙ，Ｐｅｋｉｎｇ...

关键词： landscape ecology sustainable development agrolandscape sustainabillty.

The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3.3.cells

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Cell Research 2002年第2期12卷 151-156页

作者： MENG SEN LI, PING FENG LI, FBI YI YANG, SHI PENG HE, Guo GUANG DU, GANG LI1 department of Biochemistry and Molecular Biology, 2 department of Biophysics, health Science {3., peking university, beijing 100083, China 1department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100083 China 1department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100083 China 1department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100083 China 2department of Biophysics Health Science Center Peking University Beijing 100083 China 1department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100083 China 1department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100083 China

AIM The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3.3.cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS The effect of AFP on the proliferation of NIH 3.3.cells was measured by incorporation of 3.-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3.3.cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS The proliferation of NIH 3.3.cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722×10-9M (Bmax=12810 sites per cell) and 8.93.× 10-8M (Bmax=119700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by

关键词： Alpha-fetoprotein receptor signal transduction monoclonal antibody gene expression.

Three-dimensional decomposition method of global atmospheric circulation

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Science China Earth sciences 2008年第3期51卷 386-402页

作者： LIU HaiTao1,2,3. HU ShuJuan4, XU Ming5 & CHOU JiFan6,7 1 State Key Laboratory of Numerical Modeling for Atmospheric sciences and Geophysical Fluid Dynamics, Institute of Atmospheric Physics, Chinese Academy of sciences, beijing 100029, China 2 Graduate {3. of Chinese Academy of sciences, beijing 100049, China 3.beijing Climate center, beijing 100089, China 4 department of Mathematics, Lanzhou {3., Lanzhou 73.000, China 5 Shanghai Typhoon Institute, Shanghai 20003., China 6 department of Atmospheric sciences, Lanzhou {3., Lanzhou 73.000, China 7 China Meteorological Administration Training center, beijing 100089, China 1. State Key Laboratory of Numerical Modeling for Atmospheric sciences and Geophysical Fluid Dynamics Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China 2. Graduate university of Chinese Academy of sciences Beijing 100049 China 3. beijing Climate center Beijing 100089 China 4. department of Mathematics Lanzhou University Lanzhou 730000 China 5. Shanghai Typhoon Institute Shanghai 200030 China 6. department of Atmospheric sciences Lanzhou University Lanzhou 730000 China 7. China Meteorological Administration Training center Beijing 100089 China

By adopting the idea of three-dimensional Walker, Hadley and Rossby stream functions, the global atmospheric circulation can be considered as the sum of three stream functions from a global perspective. Therefore, a m... 详细信息

关键词： three-dimensional decomposition of the global atmospheric circulation generalized Walker Hadley and Rossby stream functions existence and uniqueness vertical motion

DIFFERENT RELEASE OF OPIOID PEPTIDES IN THE HUMAN SPINAL CORD BY PERIPHERAL STIMULATION OF DIFFERENT FREQUENCIES

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Chinese Science Bulletin 1992年第6期37卷 527-528页

作者：陈晓红康妹娟赵淳department of physiology beijing {3. Unviersity (BMU) beijing 100083 PRC) 韩济生department of physiology beijing {3. Unviersity (BMU) beijing 100083 PRC) 袁毓严尚诚 TERENIUS LARS department of physiology Beijing Medical Unviersity (BMU) Beijing 100083 PRC) department of Orthopedics The First Hospital of BMU department of Pharmacology University of Uppsala Sweden.

We have shown previously that 2 Hz- and 100 Hz-electroacupuncture (EA) could induce the release of Met-enkephalin (ME) and dynorphin respectively from the spinal cord of the rat to produce analgesia. It remains to be proved whether the same principle is applicable to humans. In the present study, transcutaneous electrical nerve stimulation (TENS)

关键词： analgesia TENS stimulation lumbar applicable stimulator rubber nerves 万卿 underlying

The generation of IPSP was antagonized by injection of 6-OHDA

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Acta Pharmacologica Sinica 1999年第5期 92-93页

作者： LI Ju-Xiang, FAN Shao-Guang (department of physiology, beijing {3. university, beijing 100083, China)

In the present study 6 hydroxydopamine (6-OHDA) was used for chemical sympathectomy and the effect of 6-OHDA on generation of immune suppressive protein of stress (ISPS) was *** was found that the generation of ISPS was significantly *** maximal suppression was found on d 5 after 6-OHDA injection and it recovered gradually to normal on d *** order to assess the effectiveness of the 6-OHDA injection, the contents of norepinephrine (NE) in spleen and lymph node were assayed by high performance liquid chromatography-electrochemical detector (HPLC-ED).NE was

关键词： The generation of IPSP was antagonized by injection of 6-OHDA

A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes

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Journal of Integrative Plant Biology 2009年第8期51卷 751-761页

作者： Liming Zhou1,2, Ying Fu1,2 and Zhenbiao Yang2,3.(1State Key Laboratory of Plant physiology and Biochemistry, department of Plant sciences, College of Biological sciences, China Agricultural university, beijing 100193. China 2China Agricultural university (CAU)-university of California, Riverside (UCR) Joint center for Biological sciences and Biotechnology, College of Biological sciences, China Agricultural university, beijing 100193. China 3.8} for Plant Cell Biology and department of Botany and Plant sciences, university of California, Riverside, CA 92521, USA) ( State Key Laboratory of Plant physiology and Biochemistry Department of Plant Sciences College of Biological Sciences China Agricultural University Beijing 100193 China China Agricultural university (CAU)-university of California Riverside (UCR) Joint Center for Biological Sciences and Biotechnology College of Biological Sciences China Agricultural University Beijing 100193 China center for Plant Cell Biology and department of Botany and Plant sciences University of California Riverside CA 92521 USA)

Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. *** members of calrnodulin （CAM）, CaM-like （CML）, calcium-dependent protein kinase （CDPK） and calcineurin B-like protein （CBL） families were tagged with green fluorescence protein （GFP） and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK3.-GFP caused severe growth depolarization. CBL2-GFP and CBL3.GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane （PM）, and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes.

关键词： Arabidopsis calcium sensor calcium signaling Rho-related GTPase from plants subcellular localization tip growth