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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(Life sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry science,Beijing 100097,china 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3 college of Life science,Capital Normal university,Beijing 100037,china 4 college of Life science,Zhongkai university of Agriculture and Technology,Guangzhou 510225,china 5 college of grassland science,gansu agricultural university,lanzhou 730070,china 6 Department of Biology,Henan Institute of science and Technology,Xinxiang 453003,china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. college of Life science Capital Normal University Beijing 100037 China 4. college of Life science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. college of grassland science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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D(β)-vertex-distinguishing total coloring of graphs

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science china Mathematics 2006年第10期49卷 1430-1440页

作者： ZHANG Zhongfu,LI Jingwen,CHEN Xiang’en,YAO Bing, WANG Wenjie & QIU Pengxiang Institute of Applied Mathematic, lanzhou Jiaotong university, lanzhou 730070, china college of Mathematics and Information science, Northwest Normal university, lanzhou 730070, china college of Information and Electrical Engineering, lanzhou Jiaotong university, lanzhou 730070, china Institute of Applied Mathematic Lanzhou Jiaotong University Lanzhou 730070 China college of Mathematics Information science Northwest Normal University Lanzhou 730070 China college of Information Electrical Engineering Lanzhou Jiaotong University Lanzhou 730070 China

A new concept of the D(β)-vertex-distinguishing total coloring of graphs, i.e., the proper total coloring such that any two vertices whose distance is not larger than β have different color sets, where the color set of a vertex is the set composed of all colors of the vertex and the edges incident to it, is proposed in this paper. The D(2)-vertex-distinguishing total colorings of some special graphs are discussed, meanwhile, a conjecture and an open problem are presented.

关键词： graph, total coloring, D(β)-vertex-distinguishing total coloring, D(β)-vertexdistinguishing total chromatic number.

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On adjacent-vertex-distinguishing total coloring of graphs

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science china Mathematics 2005年第3期48卷 289-299页

作者： ZHANG Zhongfu, CHEN Xiang’en, LI Jingwen, YAO Bing, LU Xinzhong & WANG Jianfang college of Mathematics and Information science, Northwest Normal university, lanzhou 730070, china Department of Computer, lanzhou Normal college, lanzhou 730070, china Institute of Applied Mathematics, lanzhou Jiaotong university, lanzhou 730070, china college of Information and Electrical Engineering, lanzhou Jiaotong university, lanzhou 730070, china Institute of Applied Mathematics, Chinese Academy of sciences, Beijing 100080, china 1. college of Mathematics and Information science Northwest Normal University 730070 Lanzhou China 2. Department of Computer Lanzhou Normal College 730070 Lanzhou China 3. Institute of Applied Mathematics Lanzhou Jiaotong University 730070 Lanzhou China 4. college of Information and Electrical Engineering Lanzhou Jiaotong University 730070 Lanzhou China 5. Institute of Applied Mathematics Chinese Academy of Sciences 100080 Beijing China

In this paper, we present a new concept of the adjacent-vertex-distinguishing total coloring of graphs (briefly, AVDTC of graphs) and, meanwhile, have obtained the adjacent-vertex-distinguishing total chromatic number... 详细信息

关键词： graph, proper total coloring, adjacent-vertex-distinguishing total coloring, adjacent-vertex-distinguishing total chromatic number.

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Short cycle structures for graphs on surfaces and an open problem of Mohar and Thomassen

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science china Mathematics 2006年第2期49卷 212-224页

作者： REN Han & DENG Mo Department of Mathematics, East china Normal university, Shanghai 200062, china college of Mathematics & Information science, Northwest Normal university, lanzhou 730070, china 1. Department of Mathematics East China Normal University Shanghai 200062 China 2. college of Mathematics & Information science Northwest Normal University Lanzhou 730070 China

In this paper we investigate cycle base structures of a (weighted) graph and show that much information of short cycles is contained in an MCB (i.e., minimum cycle base). After setting up a Hall type theorem for base-transformation, we give a sufficient and necessary condition for a cycle base to be an MCB. Furthermore, we show that the structure of MCB in a (weighted)graph is unique. The property is also true for those having a longest length (although much work has been down in evaluating MCB, little is known for those having a longest length). We use those methods to find out some unknown properties for short cycles sharing particular properties in (unweighted) graphs. As applications, we determine the structures of short cycles in an embedded graph and show that there exist polynomially bounded algorithms in finding a shortest contractible cycle and a shortest two-sided cycle provided such cycles exist. Those answer an open problem of B. Mohar and C. Thomassen.

关键词： cycle base facial cycle graph embedding.

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Dusty cloud radiative forcing derived from satellite data for middle latitude regions of East Asia

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Progress in Natural science:Materials International 2006年第10期16卷 1084-1089页

作者： HUANG Jianping , WANG Yujie, WANG Tianhe and YI Yuhong ( college of Atmospheric sciences, lanzhou university, lanzhou 730000, china gansu Provincial Meteorological Bureau, lanzhou 730020, china) [a]college of Atmospheric sciences Lanzhou University Lanzhou China [b]gansu Provincial Meteorological Bureau Lanzhou China

The dusty cloud radiative forcing over the middle latitude regions of East Asia was estimated by using the 2-year (July 2002-June 2004) data of collocated clouds and the Earth's radiant energy system (CERES) scanner and moderate resolution imaging spectroradiometer (MODIS) from Aqua Edition IB SSF (single scanner footprint). The dusty cloud is defined as the cloud in dust storm environment or dust contaminated clouds. For clouds growing in the presence of dust, the instantaneous short-wave (SW) forcing at the top of the atmosphere (TOA) is about - 275. 7 W/m for cloud over dust (COD) region. The clouds developing in no-dust cloud (CLD) regions yield the most negative short-wave (SW) forcing ( - 311.0 W/m2), which is about 12.8% stronger than those in COD regions. For long-wave (LW) radiative forcing, the no-dust cloud (CLD) is around 102.8 W/m2 , which is 20% less than the LW forcing from COD regions. The instantaneous TOA net radiative forcing for the CLD region is about - 208.2 W/m2, which is 42.1 % larger than the values of COD regions. The existence of dust aerosols under clouds significantly reduces the cooling effect of clouds.

关键词： dust storm cloud radiative forcing global warming dust aerosol-cloud-climate interaction.

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Lie comodules and the constructions of Lie bialgebras

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science china Mathematics 2008年第6期51卷 1017-1026页

作者： ZHANG LiangYun college of science,Nanjing agricultural university,Nanjing 210095,china college of science Nanjing Agricultural University Nanjing 210095 China

In this paper,we first give a direct sum decomposition of Lie comodules,and then accord- ing to the Lie comodule theory,construct some(triangular)Lie bialgebras through Lie coalgebras.

关键词： Lie coalgebras Lie comodules Lie bialgebras triangular Lie bialgebras 16W30

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Maschke-type theorem and Morita context over weak Hopf algebras

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science china Mathematics 2006年第5期49卷 587-598页

作者： ZHANG Liangyun college of science, Nanjing agricultural university, Nanjing 210095, china Department of Mathematics, Nanjing university, Nanjing 210008, china 1. college of science Nanjing Agricultural University Nanjing 210095 China 2. Department of Mathematics Nanjing University Nanjing 210008 China

This paper gives a Maschke-type theorem over semisimple weak Hopf algebras,extends the well-known Maschke-type theorem given by Cohen and Fishman and constructs a Morita context over weak Hopf algebras.

关键词： weak Hopf algebra, smash product, Maschke-type theorem, Morita context.

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Comparison of sensible and latent heat fluxes during the transition season over the western Tibetan Plateau from reanalysis datasets

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Progress in Natural science:Materials International 2009年第6期19卷 719-726页

作者： Yang Cui, Chenghai Wang Disaster Reduction Key Laboratory of gansu Province, college of Atmospheric sciences, lanzhou university, lanzhou 730000, china [a]Disaster Reduction Key Laboratory of gansu Province College of Atmospheric Sciences Lanzhou University Lanzhou 730000 China

The sensible and latent heat fluxes during the transition season over the western Tibetan Plateau from the NCEP-1 (NCEP/NCAR Reanalysis 1), AR-Ⅱ (NCEP-DOE Reanalysis 2) and ERA40 reanalysis datasets were compared and analyzed. The results show that the phase change in soil moisture has a significant effect on the sensible and latent heat fluxes over the western Tibetan Plateau (TP) due to the freezing-thawing processes during the transition from the dry to the wet period. The uncertainties in the sensible and latent heat fluxes over the western TP are quite high in the reanalysis data, and depend largely on the success of the soil moisture simulations in the models. Improving the hydrological process simulations in the land-surface models in seasonally frozen ground and in the active frozen soil layer may be an effective way of enhancing the reliability of the surface heat fluxes from the reanalysis data over the Tibetan Plateau.

关键词： Western Tibetan Plateau Surface heat fluxes Reanalysis data Transition season Freezing-thawing process

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Study on Tibetan Chicken embryonic adaptability to chronic hypoxia by revealing differential gene expression in heart tissue

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science china(Life sciences) 2009年第3期52卷 284-295页

作者： LI Mei & ZHAO ChunJiang college of Animal science and Technology,china agricultural university,Beijing 100193,china 1. college of Animal science and Technology China Agricultural University Beijing 100193 China

Oxygen concentration is essential for appropriate *** can exert a significant impact on physiological alteration of the cell and *** Chicken(Gallus gallus) is a Chinese indigenous breed inhabiting in Tibetan areas,which is also a chicken breed living at high altitude for the longest time in the *** has developed an adaptive mechanism to hypoxia,which is demonstrated by that Tibetan Chicken has much higher hatchability than low-land chicken breeds in high-altitude areas of *** the present study,Tibetan Chicken fertilized full sib eggs were incubated up to Hamburger-Hamilton stage 43 under 13% and 21% oxygen concentration,*** Chicken and Dwarf Recessive White Chicken were used as control *** hearts in all of the 3 chicken breeds under hypoxic and normoxic conditions were isolated and hybridized to Genechip Chicken Genome Array to study molecular mechanisms underlying the adaptation to high altitude of Tibetan *** a result,50 transcripts highly expressed in hypoxia are screened *** up-regulated genes,some are involved in the gene ontology(GO) such as cell growth,cell difference,muscle contraction and signal ***,the expression levels of 21 transcripts are lower in hypoxia than those in *** down-regulated genes take part in cell communication,ion transport,protein amino acid phosphorylation and signal ***,gene enrichment analyses of these differential gene expressions are mainly associated with immune system response and ion channel activity in response to ***,the transcriptional expression profiles analyzed by hierarchical clustering and CPP-SOM software in all of the 3 different chicken breeds revealed that Tibetan Chicken is much closely related to Shouguang Chicken rather than Dwarf Recessive White *** addition,12 transcripts of Tibetan Chicken breed-specific expressed genes were identified,which seem to result in a more

关键词： adaptation chronic hypoxia gene chip chicken genome array Tibetan Chicken(Gallus gallus) differential gene expression

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Models for the peak value of mixed grasshopper population density in Ganjia of gansu,china

Models for the peak value of mixed grasshopper population de...

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ⅩⅪ International grassland Congress and the Ⅷ International Rangeland Congress

作者： Liu X N,Zhang D G,Jiang W L grassland science college,gansu agricultural university,lanzhou 730070,china

＜正＞Introduction The Ganjia high mountain grasslands of Xiahe County are an important pastoral area of gansu Province in the People’s Republic of *** outbreaks of serious proportion occur here almost every year,and these grasslands are already fragile due to degradation caused mainly by *** study used 1993-2003 sarvey data to re-evaluate the hypothesis for developing an accurate grasshopper predication model and analyzed the relationships between grasshopper occurrence and meteorological *** models have been used for estimating peak values of mixed grasshopper population density.

关键词： Xiahe Ganjia grasshopper population density peak value model

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