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Cryodamage to plasma membrane integrity in head and tail regions of human sperm

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Asian Journal of Andrology 2000年第2期2卷 135-138页

作者： Wei-Jie ZHU Xue-Gao LIU center for Reproductive Immunology Research, Jinan university, guangzhou 510632, china center for Reproductive Immunology Research Jinan University Guangzhou 510632 China

Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions ofindividual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-free hamster oocytepenetration rate. Methods: The eosin Y exclusion and the hypoosmotic swelling tests were combined to form a sin-gle test (HOS-EY test) to identify the spermatozoa with four types of membrane integrity. Results: After cryop-reservation, there was a marked decline in the percentage of spermatozoa with Type Ⅳ membrane integrity (head mem-brane intact/tail membrane intact), and a significant increase in those with Type Ⅰ (head membrane damaged/tail mem-brane damaged) and Type Ⅲ (head membrane damaged/tail membrane intact) membrane integrity (n = 50, P 4. P 0.05). Conclusion: (1) The HOS-EY test has the advan-tage of showing four patterns of membrane integrity in individual spermatozoon; (2) Cryopreservation causes a signifi-cant membrane rupture in the head and tail regions of spermatozoa; Type Ⅲ is the main transitional state of membranecryodamage; (3) Cryodamage to head and tail membrane may occur independently; the presence of an intact tail mem-brane does not necessarily indicate the intacmess of head membrane. (4. Intact membranes are closely related to post-thaw motility, but do not reflect the fertilizing potential.

关键词： spermatozoa cryopreservation cell membrane supravital staining hypoosmotic swelling test

Studies on Intramolecular Stacking Interaction of Ternary Complexes M（Ⅱ）（ATP）￣（2－） with Heteroaromatic N－Base Ligands

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Chemical Research in Chinese Universities 1994年第3期10卷 167-174页

作者： WU Fu-liai, SONG Bin, ZHANG Jie and JI Liang-nian (Chemistiy Department , Biotechnology Research center, zhongshan university, guangzhou, 5102.5)

o understand the driving forces leading to mixed-ligand complexes in biologi-cal *** complexes of M (ATP(L￣(2-) type, where M = Cu￣(2+), Ni￣(2+) orCo￣(2+) , and L= pyridine(Py) , 4.picoline, 3, 5-lutidine, isoquinoline (lq) or benzimi-dazole(Bi) in aqueous solution were studied by spectropliotometry, some of them(M=Cu￣(2+), L = isoquinoline or benzimidazole) were also separately studied hy po-tentiometric pH titration. The results show that an intramolecular stacking interac-tion exists between the heteroaromatic ring of the ligands and the purine moiety ofATP.

关键词： Stacking interaction Ternary complexes Heteroaromatic N-base ATP

The Contrast Sensitivity Function in Low Vision

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眼科学报 1992年第1期8卷 45-48页

作者： Chen-jin Jin, De-Zheng Wu, Lezheng Wu zhongshan Ophthalmic center, Sun Yat-sen university of Medical Sciences guangzhou 510060,{4. zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

The contrast sensitivity of 51 low vision patients (95 eyes) showed a substantial decrease in all spatial frequencies. The peak contrast sensitivity was shifted to 1 C/D from the normal 3-4.C/D. The high-frequency cut off is correlated to the visual acuity positively. Of the 33 patients, the preferred eye was the eye with better peak sensitivity in 28 patients (84.), while the preferred eye was the eye with better visual acuity in 22 patients (66%). It seems that the peak sensitivity is more important t...

关键词： CSF RFU low vision.

INHIBITION OF RAT LENS ALDOSE REDUCTASE BY QUERCETAGETIN AND PATULETIN

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眼科学报 1991年第1期7卷 29-30,33页

作者： Shaozhen Li Winifred Mao Xinyuan Cao Shunwei Liang zhongshan Ophthalmic center Sun Yat-sen university of Medical Sciences guangzhou,{4. Zhou Ding Naiming Li Department of Chemistry guangzhou Medical College guangzhou,{4. zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

In this paper the results of inhibition of the Aldose reductase(AR) activity on Wistar rat lens by Quercetagetin extracted from Tagetes erects Linn and by Patuletin extracted from Tagetes patula Linn are *** inhibited AR of the rat lens by 93.9% at 10^(-4.M, 76.0% at 10^(-5)M and 13.3% at 10^(-6)M. Patuletin inhibited AR of the rat lens by 100% at 10^(-1)M, 80% at 10^(-5)M and 22.7% at 10^(-6)M respectively. The results show that these two flavones are lens AR Inhibitors, but further ...

关键词： INHIBITION OF RAT LENS ALDOSE REDUCTASE BY QUERCETAGETIN AND PATULETIN RAT AR

A Study on the Compliance of the Patients with Ocular Fundus Diseases

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眼科学报 1999年第1期15卷 61-64页

作者： Hongshan Chen, Chenjin Jin, Yuanping LiThe First Affiliated Hospital, Sun Yat-sen university of Medical Science, guangzhou 510089 , china zhongshan Ophthalmic {4., Sun Yat-sen university of Medical Science, guangzhou 510060, china First Affiliated Hospital Sun Yat-sen University of Medical Science Guangzhou 510089 China

Purpose: To investigate the compliance of the patients with ocular fundus diseases with recommendation for follow-up examination after laser treatment, and the underlying reasons for ***: 53 patients with ocular fundus disease were asked to fill in a questionnaire which includes the socio-demographic characteristics, fear of the fundus disease, conception of the laser treatment and the motivation for staying healthy. Variables were compared for the compliers group and the non-compliers group by chi-square test. Result; Of the 53 subjects, 35 were classified as non-compliers and 18 were classified as compliers. There was no statistically significant difference between the two groups on all selected socio-demographic factors, conception of the laser treatment and the motivation for staying healthy. Significant difference was found between the two groups on two of those items concerning the fear of the diseases (P<0. 05). About half of the participants showed the lack of knowledge

关键词：弹性变形眼底疾病病人

Studies on Human Fetal Lens Crystallins Under Oxidative Stress and Protective Effects of Tea Polyphenols

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眼科学报 1998年第3期14卷 170-175页

作者： Hui Xiang, Suhua Pan, Shaozhen LiGuangdong Provincial Corps Hospital, Chinese People’ s Armed Police Forces, guangzhou 510010, china zhongshan Ophthalmic center, Sun Yat-sen {4. of Medical Sciences, guangzhou 510060, china Guangdong Provincial Corps Hospital Chinese People's Armed Police Forces Guangzhou 510010 China

Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4.and H2O2, after 5 or 24.hours, the crystallins were analysed with SDS-PAGE and *** : There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control ***: The results indicate that exposure of water-soluble

关键词：人胎儿晶状体晶体蛋白氧化变性抗氧化作用多酚

The Natural History of Dry Type of Age-related Macular Degeneration

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眼科学报 1993年第1期9卷 31-33页

作者： Feng Wen, De-Zheng Wu, Lezheng Wu National Ophthalmological Laboratories, Ministry of Public Health, china zhongshan Ophthalmic center, Sun Yat-Sen university of Medical Sciences {4. 510060, china National Ophthalmological Laboratories Ministry of Public Health China.

To study the natural history of dry type of age-related macular degeneration (AMD) and search for a sensitive method for detecting the development of the disease, the fundus fluorescein angiography, visual acuity, electroretinogram and FM 100-hue test were used to examine 75 eyes, 14. eyes, 73 eyes, and 94.eyes respectively. These examinations were taken at least twice during the follow-up periods. The average age was 63.2 years (50-80 years ). The average follow-up was 29.8 months with a range of 3-74....

关键词： age-related macular degeneration fundus fluorescein angiography electroretinogram color vision natural history.

THE SIGNIFICANCE OF testING PREOPERATIVE VISUAL FUNCTION IN CATARACT USING LASER INTERFEROMETRIC VISUAL ACUITY AND ERG

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眼科学报 1991年第1期7卷 21-24页

作者： De-Zheng Wu Lezheng Wu Xijing Xu Hui Chen Taiqing Luo zhongshan Ophthalmic center Sun Yat-sen university of Medical Sciences guangzhou 510060,{4. zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

tests of preoperative visual function and prediction of postoperative E chart visual acuity(ECVA) using laser interferometric visual acuity(LIVA) and electroretinogram(ERG) were performed in 16 cases(19 eyes) of cataract. The results showed that the coincident rate between preoperative LIVA and postoperative ECVA was 63.2%, and there was a parallel correlation between preoperative amplitude of photopic ERG b-wave and postoperative ECVA in 79.0% of the eyes. Comparing these two methods, the test of LIVA ...

关键词： THE SIGNIFICANCE OF testING PREOPERATIVE VISUAL FUNCTION IN CATARACT USING LASER INTERFEROMETRIC VISUAL ACUITY AND ERG

Variation of the Multifocal Visual Evoked Potential with Eccentricity in Isometropic Amblyopes

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眼科学报 1997年第3期13卷 148-155页

作者： Minzhong Yu, Brian Brown Marion EdwardsDepartment of Optometry and Radiography, The Hong Kong Polytechnic university, Hong Kong, china zhongshan Ophthalmic center, Sun Yat-sen university of Medical Sciences, and National Ophthalmological Laboratories, Ministry of Public Health, china, guangzhou 510060, china Department of Optometry and Radiography Hong Kong Polytechnic University China

Purpose: To investigate the variation of visual function in different eccentricities of the visual field in isometropic ***: The stimulus matrix containing 61 hexagons was generated on a monitor. The diameter of the entire stimulating field was approximately 13.6 deg of arc; the frame rate of the monitor was 67 Hz. Every hexagon of the display contained a number of black and white small hexagonal patches which reversed during stimulation. These hexagons were simultaneously but independently modulated in time by the controlling computer program. The flashed elements were selected differently on each frame according to a computer-generated binary pseudo-random time series (m-sequence); the response contributions from each of the individual stimulus elements could be extracted from the cross corre-ation function. Subjects were asked to maintain fixation at the center of the stimulus pattern while each of the hexagons of the display reversed. The VERB system extracted the local responses by

关键词：视觉博起电压弱视屈光相等视野离心率 VEP