Cryodamage to plasma membrane integrity in head and tail regions of human sperm

作     者：Wei-Jie ZHU Xue-Gao LIU Center for Reproductive Immunology Research, Jinan University, Guangzhou 510632, China 

作者机构：Center for Reproductive Immunology Research Jinan University Guangzhou 510632 China 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2000年第2卷第2期

页      面：135-138页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

主　　题：spermatozoa cryopreservation cell membrane supravital staining hypoosmotic swelling test 

摘      要：Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions ofindividual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-free hamster oocytepenetration rate. Methods: The eosin Y exclusion and the hypoosmotic swelling tests were combined to form a sin-gle test (HOS-EY test) to identify the spermatozoa with four types of membrane integrity. Results: After cryop-reservation, there was a marked decline in the percentage of spermatozoa with Type Ⅳ membrane integrity (head mem-brane intact/tail membrane intact), and a significant increase in those with Type Ⅰ (head membrane damaged/tail mem-brane damaged) and Type Ⅲ (head membrane damaged/tail membrane intact) membrane integrity (n = 50, P 0.05). Conclusion: (1) The HOS-EY test has the advan-tage of showing four patterns of membrane integrity in individual spermatozoon; (2) Cryopreservation causes a signifi-cant membrane rupture in the head and tail regions of spermatozoa; Type Ⅲ is the main transitional state of membranecryodamage; (3) Cryodamage to head and tail membrane may occur independently; the presence of an intact tail mem-brane does not necessarily indicate the intacmess of head membrane. (4) Intact membranes are closely related to post-thaw motility, but do not reflect the fertilizing potential.