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Studies on Intramolecular Stacking Interaction of Ternary Complexes M（Ⅱ）（ATP）￣（2.） with Heteroaromatic N－Base Ligands

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Chemical Research in Chinese Universities 1994年第3期10卷 167-174页

作者： WU Fu-liai, SONG Bin, ZHANG Jie and JI Liang-nian (Chemistiy Department , Biotechnology Research center, zhongshan university, {2., 5102.5)

o understand the driving forces leading to mixed-ligand complexes in biologi-cal *** complexes of M (ATP(L￣(2.) type, where M = Cu￣(2.), Ni￣(2.) orCo￣(2.) , and L= pyridine(Py) , 4.picoline, 3, 5-lutidine, isoquinoline (lq) or benzimi-dazole(Bi) in aqueous solution were studied by spectropliotometry, some of them(M=Cu￣(2.), L = isoquinoline or benzimidazole) were also separately studied hy po-tentiometric pH titration. The results show that an intramolecular stacking interac-tion exists between the heteroaromatic ring of the ligands and the purine moiety ofATP.

关键词： Stacking interaction Ternary complexes Heteroaromatic N-base ATP

Cryodamage to plasma membrane integrity in head and tail regions of human sperm

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Asian Journal of Andrology 2000年第2期2卷 135-138页

作者： Wei-Jie ZHU Xue-Gao LIU center for Reproductive Immunology Research, Jinan university, {2. 510632. China center for Reproductive Immunology Research Jinan University Guangzhou China

Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions ofindividual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-free hamster oocytepenetration rate. Methods: The eosin Y exclusion and the hypoosmotic swelling tests were combined to form a sin-gle test (HOS-EY test) to identify the spermatozoa with four types of membrane integrity. Results: After cryop-reservation, there was a marked decline in the percentage of spermatozoa with Type Ⅳ membrane integrity (head mem-brane intact/tail membrane intact), and a significant increase in those with Type Ⅰ (head membrane damaged/tail mem-brane damaged) and Type Ⅲ (head membrane damaged/tail membrane intact) membrane integrity (n = 50, P 4. P 2., r = 0.2., P > 0.05). Conclusion: (1) The HOS-EY test has the advan-tage of showing four patterns of membrane integrity in individual spermatozoon; (2. Cryopreservation causes a signifi-cant membrane rupture in the head and tail regions of spermatozoa; Type Ⅲ is the main transitional state of membranecryodamage; (3) Cryodamage to head and tail membrane may occur independently; the presence of an intact tail mem-brane does not necessarily indicate the intacmess of head membrane. (4. Intact membranes are closely related to post-thaw motility, but do not reflect the fertilizing potential.

关键词： spermatozoa cryopreservation cell membrane supravital staining hypoosmotic swelling test

The Contrast Sensitivity Function in Low Vision

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眼科学报 1992年第1期8卷 45-48页

作者： Chen-jin Jin, De-Zheng Wu, Lezheng Wu zhongshan Ophthalmic center, Sun Yat-sen {2. of Medical Sciences guangzhou 510060, China zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

The contrast sensitivity of 51 low vision patients (95 eyes) showed a substantial decrease in all spatial frequencies. The peak contrast sensitivity was shifted to 1 C/D from the normal 3-4.C/D. The high-frequency cut off is correlated to the visual acuity positively. Of the 33 patients, the preferred eye was the eye with better peak sensitivity in 2. patients (84.), while the preferred eye was the eye with better visual acuity in 2. patients (66%). It seems that the peak sensitivity is more important t...

关键词： CSF RFU low vision.

INHIBITION OF RAT LENS ALDOSE REDUCTASE BY QUERCETAGETIN AND PATULETIN

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眼科学报 1991年第1期7卷 29-30,33页

作者： Shaozhen Li Winifred Mao Xinyuan Cao Shunwei Liang zhongshan Ophthalmic center Sun Yat-sen {2. of Medical Sciences guangzhou, China Zhou Ding Naiming Li Department of Chemistry guangzhou Medical College guangzhou, China zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

In this paper the results of inhibition of the Aldose reductase(AR) activity on Wistar rat lens by Quercetagetin extracted from Tagetes erects Linn and by Patuletin extracted from Tagetes patula Linn are *** inhibited AR of the rat lens by 93.9% at 10^(-4.M, 76.0% at 10^(-5)M and 13.3% at 10^(-6)M. Patuletin inhibited AR of the rat lens by 100% at 10^(-1)M, 80% at 10^(-5)M and 2..7% at 10^(-6)M respectively. The results show that these two flavones are lens AR Inhibitors, but further ...

关键词： INHIBITION OF RAT LENS ALDOSE REDUCTASE BY QUERCETAGETIN AND PATULETIN RAT AR

A Study on the Compliance of the Patients with Ocular Fundus Diseases

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眼科学报 1999年第1期15卷 61-64页

作者： Hongshan Chen, Chenjin Jin, Yuanping LiThe First Affiliated Hospital, Sun Yat-sen university of Medical Science, guangzhou 510089 , China {2. Ophthalmic center, Sun Yat-sen university of Medical Science, guangzhou 510060, China First Affiliated Hospital Sun Yat-sen University of Medical Science Guangzhou 510089 China

Purpose: To investigate the compliance of the patients with ocular fundus diseases with recommendation for follow-up examination after laser treatment, and the underlying reasons for ***: 53 patients with ocular fundus disease were asked to fill in a questionnaire which includes the socio-demographic characteristics, fear of the fundus disease, conception of the laser treatment and the motivation for staying healthy. Variables were compared for the compliers group and the non-compliers group by chi-square test. Result; Of the 53 subjects, 35 were classified as non-compliers and 18 were classified as compliers. There was no statistically significant difference between the two groups on all selected socio-demographic factors, conception of the laser treatment and the motivation for staying healthy. Significant difference was found between the two groups on two of those items concerning the fear of the diseases (P<0. 05). About half of the participants showed the lack of knowledge

关键词：弹性变形眼底疾病病人

Prevention of pathological change and cognitive degeneration of Tg2.76 mice by inoculating Aβ_(1-15) vaccine

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Science China(Life Sciences) 2008年第8期51卷 743-750页

作者： HU JinJia1, LI GuoYing2. WANG HuaQiao3, LIN Xian3 & YAO ZhiBin3 1 Department of Anatomy, Medical College of Shanghai Jiao Tong university, Shanghai 2.002., China 2.Department of Anatomy, Preclinical College, Guangdong university of Pharmacy, {2. 510006, China 3 Department of Anatomy, zhongshan School of Medicine, Sun Yat-sen university, {2. 510080, China 1. Department of Anatomy Medical College of Shanghai Jiao Tong University Shanghai 200025 China 2. Department of Anatomy Preclinical College Guangdong University of Pharmacy Guangzhou 510006 China 3. Department of Anatomy Zhongshan School of Medicine Sun Yat-sen University Guangzhou 510080 China

This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2.76 mice by inoculating the subunit fragment of Aβ ***-two Tg2.76 mice were randomly divided into four groups, each having eight mice:Group I, the control group, inoculated with adjuvants;Group II, the Aβ4. group, inoculated with Aβ4. vaccine;Group Ⅲ, the Aβ1―15 group, inoculated with Aβ1―15 vaccine;and Group IV, the Aβ36―4. group, inoculated with Aβ36―4. *** titer of the serum anti-body against Aβ4.(Group II) was significantly higher than that of the control group(Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated *** water maze test showed that the Aβ4. group, Aβ1―15 group and Aβ36―4. group were obviously im-proved compared with the control *** cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control *** the Aβ4. group, IL2.and IFN-γ were relatively low and IL4.and IL10 were relatively *** contrast, IL4.and IL10 were much higher in the Aβ1―15 group and IL2.and IFN-γ were much higher in the Aβ36―4. *** immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the con-trol group, no senile plaque in the brain of the Aβ1―15 group and Aβ4. group mice, and a small number of senile plaques in the brain of the Aβ36―4. group *** results suggest that the subunit fragment of Aβ1―15 vaccine could prevent not only cognitive and behavioral degeneration but also Aβ deposition and formation of senile plaques in Tg2.76 mice.

关键词： Alzheimer’s disease β-amyloid peptide vaccine Tg2.76 mice senile plaque

THE SIGNIFICANCE OF testING PREOPERATIVE VISUAL FUNCTION IN CATARACT USING LASER INTERFEROMETRIC VISUAL ACUITY AND ERG

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眼科学报 1991年第1期7卷 21-24页

作者： De-Zheng Wu Lezheng Wu Xijing Xu Hui Chen Taiqing Luo zhongshan Ophthalmic center Sun Yat-sen {2. of Medical Sciences guangzhou 510060, China zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

tests of preoperative visual function and prediction of postoperative E chart visual acuity(ECVA) using laser interferometric visual acuity(LIVA) and electroretinogram(ERG) were performed in 16 cases(19 eyes) of cataract. The results showed that the coincident rate between preoperative LIVA and postoperative ECVA was 63.2., and there was a parallel correlation between preoperative amplitude of photopic ERG b-wave and postoperative ECVA in 79.0% of the eyes. Comparing these two methods, the test of LIVA ...

关键词： THE SIGNIFICANCE OF testING PREOPERATIVE VISUAL FUNCTION IN CATARACT USING LASER INTERFEROMETRIC VISUAL ACUITY AND ERG

Pluripotent Embryonic Stem Cells Developed into Medulloepithelioma in Nude Mice Eyes

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Eye Science 2002年第1X期18卷 37-44页

作者： Yongping Li , Xiufeng Zhong , Jianhua Yan , Jianxian Lin, Song Tan Xuan Wu, Shulong Li, Guanguang Feng, Yuzhen Yi zhongshan Ophthalmic center, Sun Yat-sen {2., guangzhou 510060, China Department of Pathophysiology, Sun Yat-sen {2., guangzhou zhongshan Ophthalmic center Sun Yat-sen University Guangzhou 510060 China

Purpose: The pluripotent embryonic stem cells can differentiate into various kinds offormal tissues. There is no previous report on the differentiation of embryonic stem cellin the intraocular environment. In this paper, the authors tried to investigate theintraocular growth character of mice embryonic stem cells in nude ***: Murine embryonic stem cells were cultured and maintained in anundifferentiated state in vitro. They were transplanted into the right eyes of 2. nude miceby microinjection under operating microscope. Animal eye observation, light microscopeand immunohistochemical examinations were ***: Two to three days after transplantation, small pieces of gray-white materialcould be viewed in the vitreous cavity. Between the 15th and 2.th day, the gray-whitemass grew into the anterior chamber in 4.nude mice eyes. Then, the mass at the anteriorchamber extended extraocularly. On the 30th day, a remarkable proptosis was observedin two of the four nude mice. In 6 to 4. days, the mice were executed for morphologicalexamination which showed the following typical structures: (1) Undifferentiated cellswith prominent nucleolius. (2. Flexner-Wintersteiner-like rosettes. (3) Medulloepithe-lioma-like structure: the cells were arranged in sheets, cords, tubes, and cysts. (4. Large,spindle-or astrocyte-like cells. (5) Cartilage-like structure. Immunohistochemically, mostof the cells were highly positive in NSE staining and a few cells were moderately positivein GFAP ***: Both animal eye findings and morphologic examinations certificated thatthe transplanted embryonic stem cells could grow in the eyes of nude mice anddifferentiate into intraocular medulloepithelioma.

关键词：胚胎干细胞移植髓上皮瘤眼肿瘤

Studies on Human Fetal Lens Crystallins Under Oxidative Stress and Protective Effects of Tea Polyphenols

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眼科学报 1998年第3期14卷 170-175页

作者： Hui Xiang, Suhua Pan, Shaozhen LiGuangdong Provincial Corps Hospital, Chinese People’ s Armed Police Forces, guangzhou 510010, China zhongshan Ophthalmic {2., Sun Yat-sen university of Medical Sciences, guangzhou 510060, China Guangdong Provincial Corps Hospital Chinese People's Armed Police Forces Guangzhou 510010 China

Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2.2.and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2.2. Then the TP was added to the solution with CuSO4.and H2.2. after 5 or 2.1}hours, the crystallins were analysed with SDS-PAGE and *** : There were marked oxidative modifications of lens protein in H2.2.and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control ***: The results indicate that exposure of water-soluble

关键词：人胎儿晶状体晶体蛋白氧化变性抗氧化作用多酚