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Methodological characteristics in establishing rat models of poststroke depression

Neural Regeneration Research 2006年第9期1卷 829-832页

作者： Fuyou Liu1, Shi Yang2, Weiyin Chen1, Jinyu Wang1, Yi Tang1, Guanxiang Zhu1 1department of Neurology, Affiliated Hospital, chengdu university of Traditional Chinese Medicine, chengdu 610072,{4. Province,{5. 2department of Neurology, First People’s Hospital of Chongqing City, Chongqing 4.0011,{5. department of Neurology Chengdu University of Traditional Chinese Medicine Chengdu 610072 Sichuan Province China department of Neurology First People's Hospital of Chongqing City Chongqing 400011 China

BACKGROUND: Ideal model of poststroke depression (PSD) may be induced in rats guided by the theoretical evidence that 'primary endogenous mechanism' and 'reactivity mechanism' theories for PSD in human being. OBJECTIVE: To investigate the feasibility of comprehensive methods to induce PSD models in rats. DESIGN: A randomized controlled animal trial. SETTING: department of Neurology, Affiliated Hospital of chengdu university of Traditional Chinese Medicine. MATERIALS: Male SD rats of SPF degree, weighing 35.-5.0 g, were provided by the experimental animal center of chengdu university of Traditional Chinese Medicine. The rats were raised for 1 week adaptively, then screened behaviorally by open-field test and passive avoidance test. Forty-five rats with close scores were randomly divided into {5. control group (n =10), simple stroke group (n =10), stress group (n =10) and PSD group (n =15.. METHODS: The experiments were carried out in the laboratory of chengdu university of Traditional Chinese Medicine from July 2002 to February 2003. ① Rat models of focal cerebral ischemia were induced by thread embolization, then treated with separate raising and unpredictable stress to induce PSD models. ②The neurologic deficit was evaluated by Longa 5.grade standard (the higher the score, the severer the neurologic deficit) and horizontal round rod test ({5. rat could stay on it for at least 3 minutes). ③ The behavioral changes of PSD rats were evaluated by the saccharin water test, open-field text and passive avoidance test, including the changes of interest, spontaneous and exploratory activities, etc. ④ The levels of monoamine neurotransmitters, including norepinephrine (NE), serotonin (5.HT) and dopamine, in brain were determined using fluorospectrophotometry. MAIN OUTCOME MEASURES: ① Score of Longa 5.grade standard; Stayed time in the horizontal round rod test; ② Amount of saccharin water consumption; Open-field text: time stayed in the central square, times wondering a

关键词： Traditional Chinese Medicine focal cerebral ischemia neurologic deficit water consumption internal carotid artery poststroke depression brain stem experimental animal water test rat model

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Effects of multidrug resistance, antisense RNA on the chemosensitivity of hepatocellular carcinoma cells

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Hepatobiliary & Pancreatic Diseases International 2006年第4期5卷 552-559页

作者： Bo Li, Tian Ye, Lei Zhao, De-Hua Li, Xing-Hua Gou, Lan-Ying Zhao, Lei Han, Lin Chen, Lu-Nan Yan and Jian-Ping Gong department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing university of Medical sciences, Chongqing 4.0010, china department of Traditional Chinese Surgery, Second Affiliated Hospital, Luzhou Medical College, Luzhou 64.000, china Genetic Engineering Laboratory, chengdu Di’ao Group Co., Ltd., chengdu 61004., china department of General Surgery, West china Hospital, sichuan university, chengdu 61004., china department of Hepatobiliary Surgery Second Affiliated Hospital Chongqing University of Medical Sciences Chongqing 400010 China.

BACKGROUND: Multidrug resistance is a major obstacle in cancer chemotherapy. We examined whether the antisense RNA of multidrug resistance gene 1 (mdr1) could reverse multidrug resistance in the human hepatocellular carcinoma (HCC) cell line SMMC7721/ADM. METHODS: The recombinant adenoviruses pAdEasy- GFP-ASmdr1 product was produced by the adenoviral vector AdEasy system, which can express antisense RNA against the mdr1 gene. Following that, the recombinant adenovirus was transfected into the P-glycoprotein- producing multidrug resistance cell line, SMMC7721/ADM human HCC cells resistant to adriamycin (ADM) and daunorubicin (DNR). In order to investigate the reversal of multidrug resistance phenotype, we measured the expression of mdr1 mRNA by RT-PCR and the production of P-glycoprotein by flow cytometry. The sensitivities for ADM and DNR SMMC7721/ADM cells were examined by [3-(4. 5.dimethylthi-azol-2-yl)-2,5.diphenyl-terazolium bromide] (MTT) analysis. RESULTS: The low-level expression of mdr1 mRNA and P-glycoprotein production were observed in parental sensitive cells SMMC/7721 in addition to the overexpressionof mdr1 mRNA and P-glycoprotein in SMMC7721/ADM cells. The transfection of antisense-RNA into SMMC7721/ ADM cells resulted in decreases of mdr1 mRNA and P-glycoprotein, but increase of drug sensitivities. The sensitivities of transfected SMMC7721/ADM cells to ADM and DNR in IC5. reduced by 31.25. and 62.96% respectively. CONCLUSIONS: Mdr1 antisense RNA can increase the sensitivities of SMMC7721/ADM cells to anticancer drug by decreasing the expression of the mdr1 gene and inhibiting P-glycoprotein expression. This strategy may be applicable to cancer patients with P-glycoportein mediated multidrug resistance.

关键词： antisense nucleic acid multiple drug resistance P-glycoprotein

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试论我国社会分配中的按“资”分配方式

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四川师范大学学报（社会科学版） 2000年第2期27卷 36-41页

作者：彭久麒四川师范大学计财处四川成都610068

个人收益分配中包含按生产要素分配或按“资”分配的内容是我国分配制度改革的重大理论突破和理论创新。物化资本和人力资本是“资”所内含的两大主要和本质构成要素。社会财富分配方式中引入按“资”分配因素对我国现阶段有效利用社会... 详细信息

个人收益分配中包含按生产要素分配或按“资”分配的内容是我国分配制度改革的重大理论突破和理论创新。物化资本和人力资本是“资”所内含的两大主要和本质构成要素。社会财富分配方式中引入按“资”分配因素对我国现阶段有效利用社会经济资源、推动经济发展和科技进步具有不可估量的积极效用。在重新审视社会财富公平分配含义的同时 ,应关注按“资”分配的消极成分并进行制度化的政策调控。

关键词：按资分配方式社会分配物化资本生产要素中国

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A Design of Controller for a Class of Networked Control Systems

A Design of Controller for a Class of Networked Control Syst...

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第二十四届中国控制会议

作者： Xiaomei Zhang~(1,2), Yufan Zheng~(3,2), Jianqiang Xu~(4.2) and Guoping Lu~5.(1. School of science, Nantong university, Nantong, 226007, china) (2. department of Computer science and Technology, East china normal university, Shanghai 200062, china) (3. department of Electrical and Electronics Engineering, The university of Melbourne, Victoria 3010, Australia) (4. department of Mathematics and Physics Teaching, Shanghai Institute of Technology, Shanghai 200233, china) (5. School of Electronic Engineering, Nantong university, Nantong, 226007, china)

＜正＞ This paper deals with the controller design of a networked control system with the presence of network-induced delay and transmitted data dropout. In view of data loss and network-induced delay, the networked control system is modelled as a stochastic switching discrete-time linear system with delay. By means of Lyapunov method, a novel delaydependent condition for the stability of the networked control system is given. Based on this condition, the desired controller is constructed through solving a set of linear matrix inequalities. An example is presented to illustrate the effectiveness of the proposed method.

关键词： networked control systems network-induced delay data dropout Lyapunov method linear matrix inequalities

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Possible Effect of 30K Proteins in Embryonic Development of Silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 355-361页

作者： Bo-Xiong ZHONG~1 Jian-Ke LI~(1.2) Jian-Rong LIN~3 Jian-She LIANG~4.Song-Kun SU~1 Hai-Sheng XU~1 Hai-Yan YAN~1 Ping-Bo ZHANG~5.Hiroshi FUJII~5. 1 Laboratory of Molecular Biology of Silkworm and Honeybee, department of Bioresource science, College of Animal science,Zhejiang university, Hangzhau 310029,{4. 2 Institute of Apicultural Research, Chinese Academy of Agricultural science, Beijing 100093,{4. 3 department of Sericulture, South{4. Agricultural university.Guangzhou 5.064.,{4. 4.Biochemistry Co. Ltd. of Zhejiang university, Hangzhou 310029.{4. 5.Faculty of Agriculture,Kyushu university, Fukuoka 812-81. Japan LaboratoryofMolecularBiologyofSilkwormandHoneybee DepartmentofBioresourceScienceCollegeofAnimalScienceZhej

The silkworm Bombyx mori possesses a 30K protein family of 3×10～4.Da,the biologicalfunctions of which have not been fully *** relationship between the 30K protein family and theembryonic development of temperature sensitive sex-linked mutant strain of silkworm was investigated bytwo dimensional polyacrylamide gel electrophoresis(2D-PAGE)and Matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS).The results show that protein spots 1-5.of the 30Kprotein family,mainly existing in normal strain,are possibly related to embryonic *** earlyconsumption of a 30K protein named 6G1-30K-1 and the accumulation of 30K proteins named 6G1-30K-3and 6G1-30K-4.are likely caused by the destruction of physiological balance in normal embryonic development,which may lead to lower hatchability of the temperature sensitive *** results suggest that reasonablemetabolism of 30K proteins is a prerequisite for the embryo's normal development.

关键词： silkworm Bombyx mori 30K protein embryonic development 2D-PAGE MALDI-TOF MS

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Role of arachidonic acid in hyposmotic membrane stretch-induced increase in calcium-activated potassium currents in gastric myocytes

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Acta Pharmacologica Sinica 2005年第10期26卷 1233-1242页

作者： Meng YANG~2, Xing-lan LI~3, Hui-ying XU~3, Jia-bin SUN~3, Bin MEI~4. Hai-feng ZHENG~3, Lian-hua PIAO~3, De-gang XING~3 Zhai-liu LI~3, Wen-xie XU~(2,5. ~2 department of Physiology, Shanghai Jiaotong university School of Medicine, Shanghai 200030 ~3 department of Physiology Yanbian university College of Medicine Yanji 133000 ~4.East{4. normal university School of Life science, Shanghai 200062,{4. departmentofPhysiology ShanghaiJiaotongUniversitySchoolofMedicineShanghai200030 departmentofPhysiology YanbianUniversityCollegeofMedicineYanji133000 EastchinanormaluniversitySchoolofLifescience Shanghai200062

Aim: To study effects of arachidonic acid （AA） and its metabolites on the hyposmotic membrane stretch-induced increase in calcium-activated potassium currents (IKCa) in gastric myocytes. Methods: Membrane currents were recorded by using a conventional whole cell patch-clamp technique in gastric myocytes isolated with collagenase. Results: Hyposmotic membrane stretch and AA increased both IK（Ca）and spontaneous transient outward currents significantly. Exogenous AA could potentiate the hyposmotic membrane stretch-induced increase in IK（Ca）The hyposmotic membrane stretch-induced increase inK（Ca）was significantly suppressed by dimethyleicosadienoic acid （100 μmol/L in pipette solution）, an inhibitor of phospholipase A2. Nordihydroguaiaretic acid, a lipoxygenase inhibitor, significantly suppressed AA and hyposmotic membrane stretch-induced increases in IK（Ca）. External calcium-free or gadolinium chloride, a blocker of stretch-activated channels, blocked the AA-induced increase in IK（Ca）significantly, but it was not blocked by nicardipine, an L-type calcium channel blocker. Ryanodine, a calcium-induced calcium release agonist, completely blocked the AA-induced increase inK（Ca）; however, heparin, a potent inhibitor of inositol triphosphate receptor, did not block the AA-induced increase in IK（Ca）. Conclusion: Hyposmotic membrane stretch may activate phospholipase A2, which hydrolyzes membrane phospholipids to ultimately produce AA; AA as a second messenger mediates Ca2+influx, which triggers Ca2+-induced Ca2+release and elicits activation of IK（Ca）in gastric antral circular myocytes of the guinea pig.

关键词： gastric myocyte arachidonic acid hyposmotic membrane stretch calcium-activated potassium current.

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土壤中价态砷的浸取方法研究

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四川师范大学学报（自然科学版） 1995年第6期18卷 45-51页

作者：赵仕林四川师范大学化学系

土壤砷的价态分析一直是个比较困难的问题，其主要原因是尚未找到行之有效的、不改变砷价态的土壤样品预处理方法。本文针对土壤砷主要以Ｆｅ、Ａｌ、Ｃａ、Ｍｇ盐的沉积物形态吸附于土壤中，选取柠檬酸铵－ＨＣｌ（ｐＨ＝５）的混合体... 详细信息

土壤砷的价态分析一直是个比较困难的问题，其主要原因是尚未找到行之有效的、不改变砷价态的土壤样品预处理方法。本文针对土壤砷主要以Ｆｅ、Ａｌ、Ｃａ、Ｍｇ盐的沉积物形态吸附于土壤中，选取柠檬酸铵－ＨＣｌ（ｐＨ＝５）的混合体系作浸取剂，利用络合和离子交换原理转换出沉积物中的金属阳离子，从而释放出Ａｓ（Ⅴ）．用超声波作震荡、萃取温度４０℃、时间５ｈ，即可达到９０％以上的萃取率。在此条件下，经各价态砷的回收试验，浸取方法的精密度试验，均取得了满意的结果。无论是浸取率、浸取时间，还是价态影响上都大大优于已有的类似研究工作。

关键词：土壤价态砷浸取

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环境样品中砷的价态分离方法研究

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四川师范大学学报（自然科学版） 1996年第3期19卷 82-87页

作者：赵仕林四川师范大学化学系

环境样品中砷的价态分离方法尚存在着一些操作繁复、仪器昂贵、或所用试剂有毒等方面的缺点，难于适应大批环境样品的监测．本文提出了用ＮＨ４ＭｇＰＯ４６Ｈ２Ｏ与ＮＨ４ＭｇＡｓＯ４６Ｈ２Ｏ生成共沉淀来分离Ａｓ（Ⅲ）与Ａｓ（Ⅴ）... 详细信息

环境样品中砷的价态分离方法尚存在着一些操作繁复、仪器昂贵、或所用试剂有毒等方面的缺点，难于适应大批环境样品的监测．本文提出了用ＮＨ４ＭｇＰＯ４６Ｈ２Ｏ与ＮＨ４ＭｇＡｓＯ４６Ｈ２Ｏ生成共沉淀来分离Ａｓ（Ⅲ）与Ａｓ（Ⅴ），经实验研究证明这种新方法具有操作简便、快速可靠、应用范围广和仪器、试剂易得等特点，能适用于土壤。

关键词：环境样品砷分离共沉淀价态分离法

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Assessment of candidate plant DNA barcodes using the Rutaceae family

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science china(Life sciences) 2010年第6期53卷 701-708页

作者： LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3,PANG XiaoHui1,YU Hua1,LI XiWen1,4.& LIU Zhen2 1Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical sciences,Beijing 100193,china 2College of Pharmacy,Hubei university of Chinese Medicine,Wuhan 4.0061,china 3School of Bioscience and Engineering,Southwest Jiaotong university,chengdu 610031,china 4.8} of Chemistry,Tsinghua university,Beijing 100084.china 1. Institute of Medicinal Plant Development Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100193 China2. College of Pharmacy Hubei University of Chinese Medicine Wuhan 430061 China3. School of Bioscience and Engineering Southwest Jiaotong University Chengdu 610031 China4. Department of Chemistry Tsinghua University Beijing 100084 China

DNA barcoding is a rapidly developing frontier technology that is gaining worldwide ***,seven regions (psbA-trnH,matK,ycf5.rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family *** evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were *** found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample *** ITS2 locus had the highest identification efficiency among all tested *** a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related ***,we propose that ITS2 is a promising candidate barcode for plant species identification.

关键词： DNA barcoding ITS2 Rutaceae identification

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Studies of Photochemical Immobilization of Urease onto Poly(ethylene terephthalate) Film Surface

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Chinese Journal of Biomedical Engineering(English Edition) 2002年第4期11卷 171-176页

作者： LI Sai 1,FU Hao 2, LUO Xiang Lin 2,ZHONG Yin Ping 21 chengdu Institute of Organic Chemistry, Chinese Academy of sciences, chengdu 61004., china 2 {4. of polymer materials, sichuan university, chengdu 610065. china

A photoactivetable 4.azidobenzoic acid was synthesized to immobilized urease onto Polyethyleneterephthalate(PET) film surface. The effects of photoactive enzyme solution concentration, temperature, PHs and time of irradiation on the relative activity of immobilized urease were investigated. The effect of reuse on the activity of immobilized urease was also studied. The chemical immobilized of urease was verified with surface elemental analysis by ESCA. The surface concentration of urease immobilized on PET surface was about 0.3 mg/cm 2. When the irradiation time was 5.minutes, the immobilized urease had the highest relative activity and the activity increased with the increase in the concentration of photoactive urease solution. The optimum pH and temperature of immobilized urease were 7 and 5.℃ respectively. The relative activity of immobilized urease was stable (5.%) after 12 times reused at 5.℃.

关键词： immobilization urease surface modification

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