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Six Specific Lysihe Residues are Crucial in Maintaining the Structure and Function of Soluble Manganese Stabilizing Protein

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Acta Biochimica et Biophysica Sinica 2006年第9期 611-619页

作者： Jin-Peng GAO~(1,2#) Feng ZHANG~(1,3#) Li ZHANG~4 Yin-Long GUO~4 Kang-Cheng RUAN~5 De-An JIANG~3* Chun-He XU~1 1 institute of Plant Physiology and Ecology,shanghai institutes for Biological sciences,chinese academy of sciences,shanghai 200032,china 2 Graduate School of the chinese academy of sciences,shanghai 200032,china 3 College of Life sciences,Zhejiang University,Hangzhou 310029,china 4 shanghai mass spectrometry center,shanghai institute of organic chemistry,chinese academy of sciences,shanghai 200031,china 5 Laboratory of Proteomics,institute of Biochemistry and Cell Biology,shanghai institutes for Biological sciences,chinese academy of sciences,shanghai 200031,china

When manganese stabilizing protein （MSP） was treated with 0.5mM N-succinimidyl propionate（NSP）,the rebinding ability and oxygen-releasing capabilities of the modified MSP were not altered,in spiteof changes of MSP surface Lys ***,far-ultraviolet circular dichroism and intrinsicfluorescence spectra analysis revealed that 0.5 mM NSP-modified MSP retained most of its native secondaryand tertiary *** of the sites of NSP modification by Staphylococcus V8protease digestion ofthe modified protein,as well as analysis by matrix-assisted laser desorption ionization-time of flight massspectrometry,indicated that seven Lys residues were *** results suggested that these residues arenot absolutely essential to the structure and function of ***,when the NSP concentration wasincreased to 4mM,the modified MSP was unable to bind photosystem Ⅱ and completely lost its *** far-ultraviolet circular dichroism and intrinsic fluorescence spectra analysis revealed a clearconformational change in MSP after 4 mM NSP treatment,suggesting that some Lys residues are involved inmaintaining the structure and function of *** by matrix-assisted laser desorption ionization-timeof flight mass spectrometry indicated that another six Lys residues,namely Lys20,Lys101,Lys196,Lys207,Lys130 （or Lys137） and Lys66 （or Lys76）,were modified by 4 mM ***,these six Lys residuesare crucial in maintaining the structure and function of soluble MSP.

关键词： chemical modification fluorescence spectrum circular dichroism spectrum manganese stabilizing protein Lys residue

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Synthesis and crystal structures of bis(cyclopentyl)gallium phenoxide dimer and bis (cyclopentyl) gallium naphthoxide dimer

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chinese Journal of chemistry 1994年第6期12卷 542-548页

作者： HAN, Ying TAO, Wen-Tian HUANG, Yao-Zeng JIN, Xiang-Lin SUN, JieLaboratory of Organometallic chemistry, shanghai institute of organic chemistry, chinese academy of sciences, shanghai 200032, china Laboratory of Organometallic chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

Tricyclopentylgallium reacted with phenol, naphthol respectively to yield phenox-ide (or naphthoxide) of biscyclopentylgallium which have been characterized by elemental analysis, IR, 1H NMR and mass spectrometry. X-ray diffraction analysis of compound 1 indicated that it belongs to the monoclinic system, space groups P21/c, with cell constants 0=9.602(3), 6=14.365(7), c=11.256(4)A, and β=97.54(3)°, Z=2(dimers), R=0.0706. Compound 2 assigned to the triclinic system, space groups P1 with cell constants a=9.392(4), 6=9.928(7), c=11.263(7) A, a=112.48(5), β=104.74(4), γ=99.95(5)°, and Z=l(dimers), R=0.0526. The molecule of 1 or 2 contains an oxygen-bridged coplanar Ga2O2 four-membered ring respectively.

关键词： Crystal structure bis(cyclopentyl)gallium aryloxide dimer.

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Investigation of Radical Cation in Electrophilic Fluorination by ESI-MS

Investigation of Radical Cation in Electrophilic Fluorinatio...

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第四届国际华夏学者分析化学研讨会

作者： Xiang Zhang, Yuanxi Liao, Rong Qian, Haoyang Wang, and Yinlong Guo shanghai mass spectrometry center, shanghai institute of organic chemistry, chinese academy of sciences, shanghai 200032, china

It was well recognized that the presence of fluorine in medicinals and plant-protection compounds could profoundly influence their biological properties. The need for a formation of carbon-fluorine bonds had stimulated the development of new synthetic methods that employ a variety of fluorinating agents. These might be classified as sources of fluoride ion (F-) or fluorine radicals (F) and as compounds that could deliver electrophilic fluorine (F+). In recent years, a number of N-F fluorinating agents, such as selectfluor, had emerged as generally safe, and easy to handle, selective sources of electrophilic fluorine.

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Studies on the mechanism of palladium (Ⅱ)-catalyzed cycloisomerization of trifluoromethyl-substituted enynols

Studies on the mechanism of palladium (Ⅱ)-catalyzed cyclois...

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中国化学会第十四届有机分析及生物分析学术研讨会

作者： QIAN Rong~1 ZHANG Ji-ming~2 GUO Yin-long~(*1) LU Long~(*2) (1.shanghai mass spectrometry center,shanghai institute of organic chemistry,chinese academy of sciences,shanghai 200032 2.Key Laboratory of Organofluorine chemistry,shanghai institute of organic chemistry,chinese academy of sciences,shanghai 200032)

Electrospray ionization mass spectrometry(ESI- MS)has great sensitivity,allowing mass measurement directly from solution,and its soft-ionization nature could minimize fragmentation,thus preserve the species of interest from *** this report,we systematical- ly studied the reaction of trifluoromethyl-substituted eny- nob catalyzed by PdCl

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Chiral Molecular Recognition of Cyclodextrin to Tropin by Electrospray Fourier Transform lon Cyclotron Resonance mass spectrometry

Chiral Molecular Recognition of Cyclodextrin to Tropin by El...

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第二届上海国际分析化学研讨会

作者： Shi-Fang REN Hao-Yang WANG Yin-Long GUOshanghai mass spectrometry center shanghai institute of organic chemistry CAS shanghai 200032 P. R. china Email

＜正＞ Electrospray fourier transform ion cyclotron resonance mass spectrometry was used to investigate chiral molecular recognition of tropin using α-cyclodextrin,β-cyclodextrin,γ-cyclodextrin, as chiral selectors. Chiral recognition ability of three cyclodextrins was revealed by the mass spectra based on the protonated complexes of tropin with cyclodextrins. The ratio of （[CD+A]/[CD]）dl to （[CD+A]/[CD]） in mass spectra of cyclodextrin and tropin indicates chiral recognition of cyclodextrin. Protonated tropin ion was formed when the dimeric complex of tropin with cyclodextrin undergo collision-induced dissociation （CID）. Chiral recognition of cyclodextrin was assessed by changing activation attenuation/activation pulse length.

关键词： cyclodextrin complex electrospray fourier transform ion cyclotron resonance mass spectrometry

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mass spectra of arylhydroxyoximes and their complexes with transition metals

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Acta Chimica Sinica(English Edition) 1987年第4期5卷 299-306页

作者： FU Gui-Xiang CHEN Yao-Huan ZHOU Chen-Ming WU Yuan-Wei XU Xiao-Yun ZENG Ming-Ying SHEN Huai-Yu shanghai institute of organic chemistry,Academia Sinica shanghai shanghai institute of organic chemistry Academia Sinica Shanghai

The EI mass spectra of several arylhydroxyoximes and of their coordinated complexeswith transition metals,Cu,Ni,Co and Fe have been studied using high resolution mass spectrometry,metastable ion measurements and the stable isotopes 15N,63Cu and 65Cu labelling to elucidate thefragmentation *** has been found that the spectra of all compounds 1-24 except Fe-complex 24 show molecular *** relative intensity of molecular ions of the complexes,whichcontained the same kind of metal and the arylhydroxyoximes with different substituents R,is closelyrelated to the electronegativity of *** all metal-complexes 9-22,the main decomposition pathwaysinvolve the successive loss of O,NO and then H2O or *** these spectra the fragmentions of non-metal-containing species resulting from the cleavage of the dioxime ligands can be discriminated.

关键词： Cu mass spectra of arylhydroxyoximes and their complexes with transition metals

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Study on the mechanism of Schiemann reaction by mass spectrometry

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Acta Chimica Sinica English Edition 1989年第5期7卷 422-430页

作者： DENG Yan shanghai institute of organic chemistry,Academia Sinica,shanghai shanghai institute of organic chemistry Academia Sinica Shanghai

Schiemann reaction of 12 aryl diazonium fluoborates was studied by mass spectrometry. The products and by-products of the reactions were identified and the possible pathways were explored. It is shown that Schiemann r... 详细信息

关键词： Study on the mechanism of Schiemann reaction by mass spectrometry

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LC-electrolyte effects in combination with pulse gradient chromatography enhance overall method performance of LC-MS-MS bioanalytical assays in supporting pharmacokinetic study for drug discovery

LC-electrolyte effects in combination with pulse gradient ch...

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中国药理学会临床药理学专业委员会会议暨第十次全国临床药理学学术会议

作者： LI Chuan NIU Wei SUN Yan WANG Li shanghai center for DMPK Research,shanghai institute of Materia Medica,chinese academy of sciences,shanghai 201203,china

Drug discovery calls for faster method development and high-throughput analysis in supporting drug metabolism and pharmacokinetic(PK)studies,whereas the rapid, sensitive,and accurate analysis of biological samples re- mains a significant *** analysis of complex bi- omatrices(***),liquid chromatography(LC) interfaced to mass spectrometry(MS)or tandem mass spectrometry(MS/MS)has been hampered by adverse ma- trix *** rapid assay development,it would be ben- eficial to improve the time-consuming method comparison and optimization steps by using generic procedures that work for a variety of ***,injudiciously combining the generic procedures including protein precip- itation for sample clean-up and electrospray ionization (ESI)for detection,as well as using conventional short- time isocratic or gradient LC,yield fast assay develop- ment,but often at the cost of decreased assay accuracy and increased risk of assay failure. We previously reported that the use of a mobile phase containing an extremely low concentration of ammonium formate(HCOONH)or formic acid(HCOOH)increased analyte ESi response and controlled against matrix effects. We designated these favorable effects‘LC-electrolyte ef- fects’.These favorable effects can be achieved in either the positive or the negative ion ESI mode,but not for at- mospheric-pressure chemical ionization(APCI).The mag- nitude of the LC-electroiyte effect on the analyte response depends on both the concentration of the electrolyte modifi- er added into the mobile phase and its identity,which is also *** addition,LC is often optimized with more emphasis on improving the analytical sensitivity by concentrating the analyte on the LC column leading to a narrow and symmetric band and achieving sufficient sepa- ration between analytes and polar matrix components to avoid adverse ion suppression or enhancement of MS-MS *** these reasons,we proposed the so-called ‘pulse gradient sy

关键词： LC-electrolyte effects pulse gradient chromatography LC/MS/MS discovery bioanalytical assay matrix effects

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香樟树种子中两种Ⅱ型核糖体失活蛋白凝集素活性的研究

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生物化学与生物物理进展 2004年第7期31卷 650-654页

作者：杨海燕李荣秀刘望夷中国科学院上海生命科学研究院生物化学与细胞生物学研究所中国科学院上海生命科学研究院生物工程研究中心

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖... 详细信息

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖于B链的糖结合活性和特异性 .对辛纳毒蛋白和新丰毒蛋白B链的凝集素活性进行了测定和比较 .红细胞凝集实验显示辛纳毒蛋白和新丰毒蛋白具有相同的细胞凝集活性 ,半抗原抑制实验发现它们都属于半乳糖型核糖体失活蛋白 ,荧光光谱法显示它们的糖结合常数也相同 .

关键词：新丰毒蛋白辛纳毒蛋白凝集素荧光光谱法

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静态顶空与顶空-SPME-气质联用法在烟用香料分析中的比较

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分析测试学报 2004年第3期23卷 115-117页

作者：王昊阳郭寅龙张正行安登魁中国科学院上海有机化学研究所上海质谱中心上海200032 中国药科大学药学院江苏南京210009

对静态顶空和顶空 -SPME -气相色谱 -质谱联用法在烟用香料分析中的应用进行了比较。通过比较发现顶空 -SPME法更适合于挥发性成分含量较低的香料的研究和质量控制。

关键词：顶空气相色谱-质谱固相微萃取(SPME) 香料

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