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Association of DRD2.polymorphisms and chlorpromazine-induced extrapyramidal syndrome in chinese schizophrenic patients

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Acta Pharmacologica Sinica 2006年第8期27卷 966-970页

作者： Sheng-nan WU~(2.3,5,6) Rui GAO~(2.3,6) Qing-he XING~(2.3,6) Hua-fang LI~4 Yi-feng SHEN~4 Niu-fan GU~4 Guo-yin FENG~4 Lin HE~(2.3,7)2.Bio-X Life Science Research center,Shanghai Jiaotong University,Shanghai 2.0030,china 3 Institute of Nutritional Sciences,SIBS,{2. Academy of Sciences,Shanghai 2.0031,china 4 Shanghai Institute of Mental health,Shanghai 2.0030,china 5 Shanghai Institute of Hypertension,Ruijin Hospital,Medical School of Shanghai Jiaotong University,Shanghai 2.002.,china Bio-X Life Science Research center Shanghai Jiaotong University Shanghai 200030 China

Aim:Extrapyramidal syndrome (EPS) is most commonly affected by typical antip-sychotic drugs that have a high affinity with the D2.***,manyresearch groups have reported on the positive relationship between the geneticvariations in the DRD2.gene and the therapeutic response in schizophrenia pa-tients as a result of the role of variations in the receptor in modulating *** this study,we evaluate the role DRD2.plays in chlorpromazine-induced EPS in schizophrenic ***:We identified seven SNP(singlenucleotide polymorphism) (-141Cins>del,TaqIB,TaqID,Ser311Cys,rs62.5,rs62.7and TaqIA) in the DRD2.gene in 146 schizophrenic inpatients (59 with EPS and 87without EPS according to the Simpson-Angus Scale) treated with chlorpromazineafter 8 *** alleles of all loci were determined by PCR (polymerase chainreaction).Results:Polymorphisms TaqlD,Ser311Cys and rs62.7 were not poly-morphic in the population recruited in the present *** statistical signifi-cance was found in the allele distribution of-141Cins>del,TaqIB,rs62.5 andTaqIA or in the estimated haplotypes (constituted by TaqIB,rs62.5 and TaqIA) inlinkage disequilibrium between the two ***:Our results did notlend strong support to the view that the genetic variation of the DRD2.gene playsa major role in the individually variable adverse effect induced by chlorpromazine,at least in chinese patients with *** results confirmed a previousstudy on the relationship between DRD2.and EPS in Caucasians.

关键词： dopamine receptors basal ganglia disease polymorphism chlorpromazine schizophrenia

Cloning and characterization of two putative seven-transmembrane receptor genes from cotton

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Progress in Natural Science:Materials International 2008年第10期18卷 1225-1231页

作者： Peng Gao Piming Zhao Juan Wang Haiyun Wang Guiling Wang Guixian Xia national Key laboratory of Plant {2.,Institute of Microbiology,chinese Academy of Sciences,Datun Road,Chaoyang District,Beijing 100101,china national center for Plant Gene Research,Beijing 100101,china national Key laboratory of Plant {2. Institute of Microbiology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 China national center for Plant Gene Research Beijing 100101 China

Using rapid amplification of cDNA ends (RACE)-PCR,two full-length cDNAs encoding putative seven-transmembrane receptors (designated Gh7TMpRI and Gh7TMpR2. were cloned from cotton *** blot and an ApaL1 restriction site polymorphism analyses revealed that Gh7TMpRI was derived from the ancestral A diploid genome,while Gh7TMpR2.was from the D subgenome. Northern blot hybridization indicated that both Gh7TMpR1 and GhTTMpR2.were expressed preferentially in the elongation phase of fiber *** of the Gh7TMpRl proteins were located within the membrane structure and displayed a punctuate pattern of *** of Gh7TMpR1 in fission yeast disrupted the polar growth and caused the formation of rounded cells. These results suggest that GhT7MpRl may play a critical role in cotton fiber development,perhaps as a signaling receptor that is involved in controlling fiber elongation.

关键词： Cotton Seven-transmembrane protein Fiber elongation Alloalleles

The relaxing ori-ter balance of Mycoplasma genomes

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Science china(Life Sciences) 2008年第2期51卷 182-189页

作者： ZHENG Xin1 & LIU ShuLin1,2.1 Department of Microbiology, Peking University health Science center, Beijing 100083, {2. 2.Department of Microbiology and Infectious diseases, University of Calgary, Calgary, T2. 4N1, Canada 1. Department of Microbiology Peking University Health Science Center Beijing 100083 China 2. Department of Microbiology and Infectious diseases University of Calgary Calgary T2N 4N1 Canada

Mycoplasma are wall-less bacteria with small genomes, which are thought to have resulted from massive genome reductive processes, during which the ori-ter balance may be disrupted. For technical difficulties, ori and ter have been located only in a few Mycoplasma strains. Using the Z curve method, we were able to locate turning points on the Mycoplasma genomes, with the minimum and maximum points co-locating with ori or ter in the reference genomes. Assuming Z curve correctly located ori and ter, we calculated the distances from ori to ter in both directions on the circular genome and calculated the ori-ter balance status. The Mycoplasma genomes were not balanced, possibly as a result of close association of Mycoplasma with hosts, where there would be no other microbes for Mycoplasma to compete with for nutrients, so fastest possible growth related to balanced genomes might not be needed by Mycoplasma, leading to a relaxing ori-ter balance.

关键词： Z curve Mycoplasma genome balance ori and ter evolution

Identification and classification of all potential hemolysin encoding genes and their products from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai

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Acta Pharmacologica Sinica 2005年第4期26卷 453-461页

作者： Yi-xuan ZHANG~(2.3,4,5) Yan GENG(2.3,4,5) Bo BI~(2.4) Jian-yong HE~4 Chun-fu WU~4 Xiao-kui GUO~3 Guo-ping ZHAO~(2.4,6,7) 2.Research center of Biotechnology,Shanghai Institutes for Biological Sciences,chinese Academy of Sciences,Shanghai 2.02.3,{2. 3 Department of Microbiology and Parasitology,Shanghai Second Medical University,Shanghai 2.002.,{2. 4 The School of Pharmaceutical Engineering,Shenyang Pharmaceutical University,Shenyang 110016,{2. 6 chinese national human genome center at Shanghai,ZhangJiang High Tech Park,Shanghai 2.12.3,{2. ResearchcenterofBiotechnology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200233

Aim:To identify and classify all potential hemolysin candidates of Leptospirainterrogans serogroup Icterohaemorrhagiae serovar ***:All of thepotential hemolysin encoding genes were characterized in *** geneswere cloned and expressed in Escherichia *** hemolytic activities of theexpressed proteins were assayed observing the hemolysis on sheep blood *** activities of the hemolysin candidates were measuredby thin-layer chromatography(TLC)and HPLC for *** and secretion of the hemolysins in L interrogans were studied byreverse transcription polymerase chain reaction,Western blot,and enzyme-linkedimmunosorbent *** and Conclusion:The hemolytic activities ofhemolysin candidates(LA032.,LA0378,LA102.,LA102.,LAI650,LA3050,LA3937,LA4004)from L interrogans strain Lai were *** were furtherdivided into two groups,sphingomyelinase hemolysins and non-sphingomyelinasehemolysins,based on their ability to hydrolyze *** of thesehemolysins were actually expressed in living L interrogans and some of themwere secreted into the *** study establishes an essential findcomplete basis for further studying the contribution of hemolysins to the patho-genesis of L interrogans.

关键词： hemolysin Leptospira interrogans sphingo myelinase

Fullerenes as unique nanopharmaceuticals for disease treatment

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Science china Chemistry 2010年第11期53卷 2233-2240页

作者： MA HuiLi & LIANG Xing-Jie chinese Academy of Sciences Key laboratory for Biomedical Effects of Nanomaterials and Nanosafety {2. center for Nanoscience and Technology,Beijing 100190,china 1. chinese Academy of Sciences Key laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology Beijing 100190 China

As unique nanoparticles,fullerenes have attracted much attention due to their unparalleled physical,chemical and biological *** functionalized fullerenes with OH,NH2.COOH,and peptide modifications were *** summarized the biological activities of fullerenes derivatives in cancer therapy with high efficiency and low toxicity,as reactive oxygen species scavenger and lipid peroxidation inhibitor,to inhibit human immunodeficiency virus and to suppress bacteria and microbial at low *** addition,the mechanism for fullerene to enter cells and biodistribution of fullerene in vivo was also *** research focuses on the current understanding of fullerenes-based nanomaterials in the potential clinical application as well as biological mechanism of fullerenes and its derivatives in disease therapy.

关键词： fullerene nanoparticle nanomedicine clinical application biological effect

In vitro and in vivo induction of bone formation based on adeno-associ-ated virus-mediated BMP-7 gene therapy using human adipose-derived mesenchymal stem cells

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Acta Pharmacologica Sinica 2007年第6期28卷 839-849页

作者： Yan KANG~2.Wei-ming LIAO~(2.5),Zhen-hua YUAN~4,Pu-yi SHENG~2.Long-juan ZHANG~3,Xiang-wei YUAN~2.Lei LEI~2.2.Department of Orthopedics and ~3 laboratory of General Surgery,the First Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510080,china,~4 {2. Key laboratory of Viral Genetic Engineering,{2. Institute for Viral disease Control and Prevention,chinese center fordisease Control and Prevention,Beijing 100086,china DepartmentofOrthopedicstheFirstAffiliatedHospital SunYat-SenUniversityGuangzhou510080China laboratoryofGeneralSurgery theFirstAffiliatedHospitalSunYat-SenUniversityGuangzhou510080China

Aim:To determine whether adeno-associated virus (AAV)-2.mediated,bone mor-phogenetic protein (BMP)-7-expressing human adipose-derived mesenchymal stemcells (ADMS) cells would induce bone formation in vitro and in ***:ADMS cells were harvested from patients undergoing selective suction-assistedlipectomy and transduced with AAV carrying the human BMP-7 ***-trans-duced cells and cells transduced with AAV serotype 2.carrying the enhancedgreen fluorescence protein gene served as *** cells were qualita-tively assessed for the production of BMP-7 and osteocalcin,and subjected toalkaline phosphatase (ALP) and {2.lizarin staining.A total of 2.5~ 106 cellsmixed with type Ⅰ collagen were implanted into the hind limb of severe combinedimmune-deficient (SCID) mice and subjected to a histological analysis 3 weekspost ***:Transfection of the ADMS cells achieved an effi-ciency of 99% at d *** with AAV2.BMP-7 induced the expression ofBMP-7 until d 56,which was markedly increased by d *** cells were positivelystained for *** production and matrix mineralization further con-firmed that these cells differentiated into osteoblasts and induced bone formationin vitro.A histological examination demonstrated that implantation of BMP-7-expressing ADMS cells could induce new bone formation in ***:The present in vitro and in vivo study demonstrated that human ADMS cellswould be a promising source of autologous mesenchymal stem cells for BMPgene therapy and tissue engineering.

关键词： adipose-derived mesenchymal stem ceils adeno-associated virus bone morphogenetic protein gene therapy ectopic bone formation

局部晚期非小细胞肺癌放疗靶区勾画和计划设计指南

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中华放射肿瘤学杂志 2024年第10期33卷 877-892页

作者：国家癌症中心/国家肿瘤质控中心朱广迎毕楠不详中日友好医院放射肿瘤科北京100029 国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院放疗科北京100021

局部晚期非小细胞肺癌(NSCLC)占所有NSCLC患者总数的1/3,包括ⅢA期(可手术切除和不可手术切除)、ⅢB期、ⅢC期患者。放射治疗是局部晚期NSCLC的主要治疗之一。对于不可切除局部晚期NSCLC,以放疗为基础的综合治疗使得患者5年生存率达到42...

局部晚期非小细胞肺癌(NSCLC)占所有NSCLC患者总数的1/3,包括ⅢA期(可手术切除和不可手术切除)、ⅢB期、ⅢC期患者。放射治疗是局部晚期NSCLC的主要治疗之一。对于不可切除局部晚期NSCLC,以放疗为基础的综合治疗使得患者5年生存率达到42.9%。对于可切除ⅢA期患者,放疗的价值受到挑战,但多项回顾性研究和真实世界研究的部分高危患者依然可以从术后放疗中获益,提高生存率。整体上该期患者由于异质性较大,预后的个体差异较大。中国不同放射治疗中心的NSCLC诊疗常规、靶区勾画规范、计划设计规范仍有较大差异,医生、物理师的具体工作标准也可能存在差异。精准靶区勾画是放疗获益的重要前提,为了促进肺癌临床放疗的规范化、便于开展多中心临床研究,国家肿瘤质控中心参考国内外已发布的相关标准制定此精准放疗技术下局部晚期NSCLC根治性同步放化疗和术后放疗路径、适应证、靶区勾画和计划评价指南,旨在为国内各放疗单位提供参考。

关键词：癌,非小细胞肺局部晚期术后放射疗法靶区勾画计划设计

Progress in the development of therapeutic antibodies targeting prion proteins and β-amyloid peptides

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Science china(Life Sciences) 2010年第8期53卷 959-963页

作者： HE JinSheng1, ZHANG Ying1 & HONG Tao1,2.1College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, china 2.nstitute for Viral disease Control and Prevention, {2. center for disease Control and Prevention, Beijing 100052. china 1. College of Life Sciences and Bioengineering School of Science Beijing Jiaotong University Beijing 100044 China2. Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Prion diseases and Alzheimer’s disease (AD) are characterized by protein misfolding, and can lead to dementia. However, prion diseases are infectious and transmissible, while AD is not. The similarities and differences between these diseases have led researchers to perform comparative studies. In the last 2.decades, progress has been made in immunotherapy using anti-prion protein and anti-β-amyloid antibodies. In this study, we review new ideas and strategies for therapeutic antibodies targeting prion diseases and AD through conformation dependence.

关键词： prion Alzheimer’s disease immunotherapy antibody conformation

Characterization of codon usage bias in the dUTPase gene of duck enteritis virus

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Progress in Natural Science:Materials International 2008年第9期18卷 1069-1076页

作者： Lichan Zhao a, Anchun Cheng a,b,, Mingshu Wang a,b,, Guiping Yuan c, Mingsheng Caia a Avian disease Research center, College of Veterinary Medicine of Sichuan Agricultural University, Xinkang Road 46, Ya’an, Sichuan 62.014, {2. b Key laboratory of Animal disease and human health of Sichuan Province, Ya’an 62.014, {2. c Analytical & Testing center, Sichuan University, Chengdu 610064, {2. Avian disease Research center College of Veterinary Medicine of Sichuan Agricultural University Xinkang Road 46 Ya’an Sichuan 625014 China Key laboratory of Animal disease and {2. health of Sichuan Province Ya’an 625014 China Analytical & Testing center Sichuan University Chengdu 610064 China

A comparative analysis of the codon usage bias in the newly discovered dUTPase gene (Assigned Accession No.:DQ486149) of the duck enteritis virus (DEV) and the dUTPase gene of 32.reference herpesviruses was *** results indicated that the DEV dUT-Pase gene encodes a protein of 477 amino acids, which includes five conserved motifs with a 3-1-2.4-5 *** codon adap-tation index (CAI), efiective number of codons (ENC), and GC3S values indicated synonymous codon usage bias in the dUTPase gene of herpesviruses, and this synonymous bias was correlated with host *** codon usage patterns of the DEV dUTPase gene were phylogenetically conserved and similar to that of the dUTPase genes of the avian *** codon usage in each micro-organism was difierent, there were no strain-specific difierences among ***-one codons in the predicted polypeptide, with a strong bias towards A and T at the third codon position, were *** of the codon usage in the dUTPase gene of difierent organisms revealed that there were 19 codons showing distinct codon usage difierences between the DEV and Escherichia coli dUTPase genes;16 between the DEV and yeast dUTPase genes;and 15 between the DEV and human dUTPase *** of variance (ANOVA) showed significant difierences between the DEV and yeast dUTPase genes (r = 0.536, P < 0.01).The extent of codon usage bias in the DEV dUTPase gene was highly correlated with the gene expression level, therefore the results may provide useful information for gene classification and functional studies.

关键词： DEV dUTPase Codon usage bias EMBOSS