检索结果-南通市图书馆

Role of inositol 1,4,5-trisphosphate receptors in α1-adrenergic receptorinduced cardiomyocyte hypertrophy

Acta Pharmacologica Sinica 2006年第7期27卷 895-900页

作者： Da-li LUO~(2,3,5) Jian GAO~2 Xiao-mei LAN~3 Gang WANG~4 Sheng WEI~4 Rui-ping XIAO~4 Qi-de HAN~32 Department of Pharmacology,School of Chemical Biology & Pharmaceutical sciences,Capital university of Medical sciences,beijing 100069,china 3 institute of Cardiovascular science at Health science Center,peking university beijing 100083,china 4 institute of molecular medicine and college of life science,peking university,beijing 100871,china Department of Pharmacology School of Chemical Biology & Pharmaceutical Sciences Capital University of Medical Sciences Beijing China institute of Cardiovascular science at Health science Center Peking University Beijing China institute of molecular medicine and college of life science Peking University Beijing China

Aim:Intracellular Ca2+plays pivotal roles in diverse cellular functions,includinggene transcription that underlies cardiac remodeling during stress ***,the role of inositol 1,4,5-trisphosphate receptors（IP3Rs）in the media-tion of cardiac intracellular Ca2+and hypertrophic growth remains *** with neonatal rat ventricular myocytes suggests that activation of IP3Rsmay be linked to α1adrenergic receptor（α1AR）increased stereotyped Ca2+sparkoccurrence and global Ca2+***,we hypothesized that Ca2+releasethrough IP3Rs was necessary for α1AR-stimulated cardiac ***:We used myoinositol 1,4,5-trisphosphate hexakis（butyryloxymethyl）ester（IP3BM）,a membrane-permeant ester of IP3,to activate IP3Rs directly,and Fluo 4/AM tomeasure intracellular Ca2+***:IP3BM(10μmol·L-1)mimicked theeffects of phenylephrine,a selective agonist of α1AR,in increments in local Ca2+spark release（especially in the perinuclear area）and global Ca2+*** importantly,IP3R inhibitors,2-aminoethoxydiphenyl borateand Xestospongin C,abolished the IP3BM-induced Ca2+responses,and signifi-cantly suppressed α1AR-induced cardiomyocyte hypertrophy assayed by cellsize,[3H]leucine incorporation and atrial natriuretic factor gene expression,dur-ing sustained（48 h）phenylephrine ***:These results,therefore,provide cellular mechanisms that link IP3R signaling to α1AR-stimulatedgene expression and cardiomyocyte hypertrophy.

关键词： inositol 1,4,5-trisphosphate receptors Ca2+sparks α1 adrenergic stimulation cardiac hypertrophy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

In vitro reassembly of nuclear envelopes and organelles in Xenopus egg extracts

引用

Cell Research 2006年第7期16卷 632-640页

作者： Ping Lu~1 Hui Zheng~2 Zhonghe Zhai ~1college of life sciences ~2institute of molecular medicine,peking university beijing 100871,china college of life sciences Peking University Beijing China institute of molecular medicine Peking University Beijing China

We reconstituted bilayer nuclear membranes,multilayer membranes,and organelles from mixtures of Xenopus laevis eggextracts and demembranated Xenopus sperm *** proportions of the cytosolic and vesicular fractions from theeggs were used in the reconstitution mixtures.A cytosol:vesicle ratio of 10:1 promoted reassembly of the normal bilayernuclear membrane with inserted nuclear pore complexes around the decondensed Xenopus sperm chromatin.A cytosol:vesicle ratio of 5:1 caused decondensed and dispersed sperm chromatin to be either surrounded by or divided by unusualmultilayer membrane structures with inlaid pore complexes.A cytosol:vesicle ratio of 2.5:1 promoted reconstitution ofmitochondria,endoplasmic reticulum networks,and Golgi *** reassembly of the endoplasmic reticulumand Golgi apparatus,vesicular fragments of the corresponding organelles fused together and changed their shape to formflattened cisternae,which were then stacked one on top of another.

关键词： cell-free system endoplasmic reticulum Golgi apparatus mitochondria nuclear reconstitution Xenopus egg extracts

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A New Competitive Inhibitory Monoclonal Antibody to Carboxypcptidase A(CPA)

引用

Chinese Chemical Letters 1999年第2期10卷 121-122页

作者： Mei DU Sheng JIN Zhen Quan GUO(college of Chemistry and molecular Engineering. peking university. 100871)(college of life science, peking university, 100871) peking Univ Coll Chem & Mol Engn Beijing 100871 Peoples R China peking Univ Coll Life Sci Beijing 100871 Peoples R China

The monoclonal antibody ID11D7 to carboxypeptidase A (CPA) was prepared. Theinhibitory effect of McAb ID11D7 on the peptidase activity of CPA was measured. The result reflectsthat the McAb ID11D7 can competitively inhibit the peptidase activity of CPA with an apparentinhibitory constant of 6.3 × 10-9M. Based on this work. the McAb ID11D7 can be used as idiotypicantigen to prepare anti-idiotypic catalytic antibody with the peptidase activity similar to CPA.

关键词： Monoclonal antibody carboxypeptidase A (CPA) competitive inhibition peptidaseactivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

PROPERTIES OF POLYURETHANE ELASTOMERS BASED ON POLY (OXYETHYLENE-co-OXYTETRAMETHYLENE) DIOL

引用

Chinese Journal of Polymer science 2001年第4期19卷 371-376页

作者： Chen-yu Su Guang-li Zhang Hong-zhi Zhang Department of Polymer science and Engineering college of Chemistry and molecular Engineering peking university beijing 100871, china 北京大学北京

Polyurethane elastomers derived from 4,4 ' -methylene bis(phenyl isocyanate), butylene glycol and the low oxyethylene moiety content copolyether of tetrahydrofuran and ethylene oxide, obtained by copolymerization using heteropolyacid-ethylene oxide initiator system, were prepared. The polyurethanes exhibited an increased water absorption and much better low temperature resilience, which remained high even at a temperature of -3 degreesC, while that of polyurethane based upon poly(tetramethylene ether) glycol of the 'same molecular weight, i.e. being 2000, and molecular weight distribution, i.e, in the range of 1.4-1.5, starts to decrease dramatically at 5 degreesC.

关键词： polyurethane poly(oxyethylene-co-oxytetramethylene) diol low temperature resilience heteropolyacid-ethylene oxide initiator system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibitory effect of picroside Ⅱ on hepatocyte apoptosis

引用

Acta Pharmacologica Sinica 2005年第6期26卷 729-736页

作者： Hua GAO, Ya-wei ZHOU~2 college of Chemistry and molecular Engineering, peking university, beijing 100871, china college of Chemistry and molecular Engineering Peking University Beijing China

Aim: To investigate the influence of picroside on hepatocyte apoptosis and its mechanism. Methods: Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis. Semi-quantitative reverse transcription-PCR (RT-PCR) was used to analyze the expression of bcl-2 and bax genes. The content of manganese-superoxide dismutase (SOD) in liver mitochondria was detected by the Marland method. The content of malonic aldehyde (MDA) and the protein level in liver tissue were determined by thiobarbituric acid colorimetry and Lowry method. Results: Picroside decreased the levels of alanine aminotransferase and aspartate aminotransferase in the serum resulting from acute-liver injured mice induced with D-GalN and LPS; it also reduced the content of MDA, and thus, enhanced the activity of SOD. Picroside 0 mg/kg was found to protect hepatocytes against apoptosis in a dose-dependent manner; it up-regulated the expression of bcl-2 genes, thus increased the bcl-2/bax ratio. Conclusion: Picroside 鈪an protect hepatocytes against injury and prevent hepatocytes from apoptosis. It might by upregulating the bcl-2 gene expression and antioxidation.

关键词： picroside Ⅱ hepatocytes apoptosis oxidative stress bcl-2 genes bax genes

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A NOVEL PHOTO-INITIATING SYSTEM FOR ATOM TRANSFER RADICAL POLYMERIZATION OF STYRENE

引用

Chinese Journal of Polymer science 2001年第5期19卷 441-445页

作者： Shu-hui Qin Dong-qi Qin Kun-yuan Qiu Department of Polymer science and Engineering college of Chemistry and molecular Engineering peking university beijing 100871, china 北京大学北京

A novel photo-induced initiating system, 2,2-dimethoxy-2-phenylacetophenone (DMPA)/ferric tri(NN-diethyldithiocarbamate) [Fe(DC)(3)], was developed and used for the atom transfer radical polymerization (ATRP) of styrene in toluene. The polymerization proceeds with DMPA as photo-initiator, Fe(DC)(3) as catalyst and DC as a reversible transfer group, while the halogen and ligands are free. Well-defined PSt was prepared and the polymerization mechanism revealed by end group analysis belongs to a reverse ATRP. Block copolymer was prepared by using thus obtained PSt as macroinitiator and Fe(DC)(2) as catalyst under UV light irradiation via a conventional ATRP process.

关键词： photo atom transfer radical polymerization living radical polymerization well-defined polystyrene block copolymer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SYNTHESIS AND CHARACTERIZATION OF A NOVEL BLUE LIGHT EMITTING ROD-COIL DIBLOCK OLIGOMER

引用

Chinese Journal of Polymer science 2002年第5期20卷 401-407页

作者： Lifang Yang Haifeng He Hongqing Cao Xinhua Wan Qi-feng Zhou Department of Polymer science & Engineering college of Chemistry & molecular Engineering peking university beijing 100871, china Department of Polymer science & Engineering College of Chemistry & Molecular Engineering Peking University 北京 100871 Department of Polymer science & Engineering College of Chemistry & Molecular Engineering Peking University 北京 100871

Four new rod-coil diblock molecules, which had well-defined para-phenylene oligomers (PPP, from biphenyl to quinquephenyl) as rigid segments and poly(ethylene oxide) (PEO, M-w ca. 750) as flexible segments, were synthesized. The chemical structure and the number of repeating unit in PEO coil and PPP rod were characterized by H-1-NMR and MALDI-TOF mass spectrometry. The diblock oligomers showed good solubility in common organic solvents and aggregated in water as characterized by a blue shift in UV-vis absorption spectra, a red shift in emission spectra, quenching of the fluorescence and formation of the microfibrilla and fractal-like structures. The formation of different supramolecular structures meant that this effort might lead to commercial important highly organized functional materials.

关键词： rod-coil diblock oligomer MALDI-TOF mass spectrometry microfibrilla aggregate fractal-like pattern

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Structural Analysis and Identification of Cis-Elements of Rice osRACD Gene

引用

Acta Biochimica et Biophysica Sinica 2004年第3期36卷 191-198页

作者： Yun-Zhe XU, Rui-Lin YOU and Nai-Hu WU institute of Genetics and Developmental Biology, Chinese Academy of sciences, beijing 100080, china 2college of life sciences, peking university, beijing 100871, china institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100080 China college of life sciences Peking University Beijing 100871 China

The osRACD gene correlated with fertility transformation in the photoperiod sensitive genic male sterile rice (PGMR), Nongken 58S, encoded a rice (Oryza sativa L. ssp. japonica) small GTPase belonging to the Rac/Rho family. Inverse PCR was performed to amplify a fragment about 1.4 kb in 5' upstream region of the osRACD promoter. Deletion mutation and gel mobility shift assay characterized two fragments (–799 to –686 nt, and –686 to –431 nt) in the osRACD promoter that could be involved in its transcriptional regulation. When these two deletion fragments were used as probe respectively, a retarded band appeared in the nuclear extracts of fertile 58S rice under short day (58S-SD). Whereas no retarded band was shown in the nuclear extracts of sterile 58S rice under long day (58S-LD). Competition assay indicated that the factors in the retarded bands binding to these two fragments were the same trans-acting factor (termed rice factor, RF). The binding affinity of RF was affected by phosphorylation and was higher in SD-growth rice than that of LD-growth rice.

关键词： photoperiod-sensitive male genic sterile rice osRACD gene photoperiod fertility transformation cis-element

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

“LIVING”/CONTROLLED RADICAL POLYMERIZATION OF ETHYL METHYLACRYLATE WITH 2,2’-AZOBISISOBUTYRONITRILE/FERRIC CHLORIDE/TRIPHENYLPHOSPHINE INITIATION SYSTEM

引用

Chinese Journal of Polymer science 2000年第2期18卷 149-153页

作者： Xiao-ping Chen Kun-yuan Qiu Department of Polymer science and Engineering, college of Chemistry, peking university, beijing 100871, china 北京大学北京

'Living'/controlled radical polymerization of ethyl methacrylate (EMA) was carried out with a 2,2'-azobisisobutyronitrile (AIBN)/ferric chloride (FeCl_3)/triphenylphosphine (PPh_3) initiation system at 85℃. Thc numberaverage molecular weight (M_n) increases linearly with monomer conversion and the rate of polymerization is first order withrespect to monomer concentration. The M_w of PEMA ranges from 3900 to 17600 and the polydispersity indices are quitenarrow (1.09～1.22). The conversion can reach up to～100% and M_w of the polymers obtained is close to that designed. Thepolymerization mechanism belongs to the reverse atom transfer radical polymerization (ATRP). The polymer was end-functionalized by chlorine atom, which acts as a macroinitiator to proceed extension polymerization in the presence ofCuBr/bipy catalyst system via an ATRP process. The presence of ω-chlorine in the PEMA obtained was identified by ~1H-NMR spectrum.

关键词： 'Living'/controlled radical polymerization Reverse ATRP Ethyl methacrylate AIBN/FeCl_3/PPh_3 Bulk polymerization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Antiviral compounds and one new iridoid glycoside from Cornus officinalis

引用

Progress in Natural science:Materials International 2006年第2期16卷 142-146页

作者： WANG Yue, LI Zhengquan, CHEN Lirong and XU Xiaojie (college of Chemistry and molecular Engineering, peking university, beijing 100871, china) college of Chemistry and molecular Engineering Peking University Beijing 100871 China

From Cornus officinalis Sieb. et Zucc., bioassay-guided fractionation led to the isolation of four active tannin compounds with high effectiveness of inhibiting Hepatitis C virus NS3 serine protease in vitro. The compounds are: 1,2,3, 6-tetragalloyl-β-D-glucopyranose (1), 1,2,3,4,6- pentagalloyl-β-D-glucopyranose (2), Tellimagrandin I (3) and Tellimagrandin II(4). The four compounds could inhibit HCV NS3 protease in vitro with IC50 values of 6.98, 5.11, 7.0 and 4.8μmol/L respectively. In addition, a new iridoid glycoside (5) was also isolated from Cornus officinalis Sieb. et Zucc., which was assigned to be 7-O-butyl morroniside by spec-troscopic analysis.

关键词： inhibitor HCV NS3 protease iridoid glycoside Cornus officinalis Sieb. et Zucc.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：