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pH effect of· OH radical induced oxidation of phosphoryl- methionine in aqueous solution

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chinese Science Bulletin 1997年第4期42卷 296-301页

作者： CHUGaosheng, YAO Side, ZHANG Zhicheng, ZHANG Manwei and WANG Wenfeng1.Department of applied Chemistry, University of Science and Technology of china, Hefei 230026, china 2 shanghai institute of Nuclear Research, chinese academy of sciences, shanghai 201800, china Department of applied Chemistry University of Science and Technology of China Hefei China shanghai institute of Nudear Research Chinese Academy of Sciences Shanghai China

STUDIES of radiation chemistry on methionine (Met) were very active in previous years because of its actions of radioprotection to biological systems by quenching·OH radicals. On the other hand, people have paid more and more attention to the researches on the phosphorylation of the biological polymolecules. It is wall known that phosphate ester is regarded as the deposi-

关键词： pulse radiolysis NDM a-amino radical pH effect.

The first protein ever synthesized in vitro—a personal reminiscence of the total synthesis of crystalline insulin

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Science china(Life sciences) 2010年第1期53卷 16-18页

作者： ZHANG YouShang institute of Biochemistry and Cell Biology,shanghai institutes for Biological sciences,chinese academy of sciences shanghai 200031,china 1. institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The total synthesis of crystalline bovine insulin started in 1958 was fully accomplished in 1965. This formidable task was a collaboration of the institute of Biochemistry in shanghai,

关键词： The first protein ever synthesized in vitro a personal reminiscence of the total synthesis of crystalline insulin

An Effective Method for Raising Antisera Against β-defensins: Double-copy Protein Expression of mBin1b in E. coli

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 571-576页

作者： Li-Qing XIAO, Ai-Hua LIU, and Yong-Lian ZHANG State Key Laboratory of Molecular Biology, institute of Biochemistry and Cell Biology, shanghai institutes for Biological sciences, chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 200031, china State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

Bin1b is a rat epididymis specific β-defensin which may have fertility related functions in addition to its antimicrobial activity. β-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new β-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of β-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful tool for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the β-defensin gene family.

关键词： Bin1b epididymis β-defensin double-copy protein polyclonal antiserum

Progress in glial cell studies in some laboratories in china

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Science china(Life sciences) 2010年第3期53卷 330-337页

作者： DUAN ShuMin institute of Neuroscience,State Key Laboratory of Neuroscience,shanghai institutes for Biological sciences,chinese academy of sciences,shanghai china,200031 1. institute of Neuroscience State Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China 200031

Glial cells in the central nervous system(CNS) consist of a heterogeneous population of cell types,each characterized by distinct morphological features,physiological properties,and specific *** contrast to the previous view that glial cells were passive elements in the brain,accumulating evidence suggests that glial cells are active participants in various brain functions and brain *** review summarizes recent progress of glial cell studies from several groups in china,ranging from studies about the mechanisms of neuron-glia crosstalking to investigations on the roles of glial cells in various CNS disorders.

关键词： neuron-glia interaction ATP plasticity pain ischemia neural injury and repair

A pulse radiolysis study on electron affinity of piperonal

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Science china Chemistry 2005年第5期48卷 476-480页

作者： MA Jianhua1, LIN Weizhen2, WANG Wenfeng2 & YAO Side2 1. College of Biology Engineering, Jimei University, Xiamen 361021, china 2. shanghai institute of applied physics, chinese academy of sciences, shanghai 201800, china 1. College of Biology Engineering Jimei University 361021 Xiamen China 2. shanghai institute of applied physics Chinese Academy of Sciences 201800 Shanghai China

The piperonal electron affinity was studied using pulse radiolysis technique. The electron transfer reaction process between piperonal and anthraquinone-2-sulfate was observed in the pH 7 phosphoric acid salt buffer. The transient absorption spectra of electron transfer re-action between piperonal and anthraquinone-2-sulfate were obtained, and the initial proof of the electron transfer between electron donor and acceptor was provided directly. The one-electron reduction potential of piperonal was determined to be ?0.457 V.

关键词： piperonal, pulse radiolysis, one-electron reduction potential.

Molecular Dynamics Simulation of Zero-Fluence and Low-Bombarding-Energy ^(63)Cu-^(65)Cu Sputtering

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Journal of Materials Science & Technology 1999年第2期15卷 184-186页

作者： Liping ZHENG Shi QIU and Yukui ZHOU (shanghai institute of Nuclear Research, chinese academy of sciences, P.O.Box800-204, shanghai 201800,china)Hisheng LI(Lab. of Atomic Imaging of Solids, institute of Metal Research chinese academy of sciences, Shenyang shanghai institute of Nuclear Research Chinese Academy of Sciences P.O.Box800-204 Shanghai 201800 China Lab. of Atomic Imaging of Solids Institute of Metal Research Chinese Academy of Sciences Shenyang 110015 China

A molecuIar dynamics simulation has been used to study zero-fluence and low- bombarding63Cu-65Cu sputtering. Calculations show that the isotopic ratios at both θ≤35° and energy 63Cu-65Cu sputtering. Calculations show that the isotopic ratios at both θ≤35° and θ >35°, and the total isotopic ratio increase when the bombarding energy decreases. This result might impty the existence of the bombarding-energy-dependent momentum asymmetry.

关键词： Simulation Cu Sputtering Molecular Dynamics Simulation of Zero-Fluence and Low-Bombarding-Energy

mad-overexpression down regulates the malignant growth and p53 mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells

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Cell Research 1999年第1期9卷 51-59页

作者： ZHAO HUA YONG HUA XU (shanghai institute of Cell Biology, chinese academy of sciences, 320 yue-yang Road, shanghai 200031, china) 1shanghai institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1shanghai institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Mad protein has been shown as an antagonist of cMyc protein in some cell lines. The effect of Mad protein to the malignant phenotype of human hepatoma BEL7404 cell line was investigated experimentally. An eukarryotic vector pCDNA Ⅲ containing full ORF fragmentof mad cDNA was transfected into targeted cells. Under G418 selection, stable Mad-overexpressed cells were *** on the effect of Mad over-expression in cell proliferation and cell cycle revealed that cell morphology of the Mad-overexpressed BEL-7404-M1 cells was significantly different from the parent and control vector transfected cells. DNA synthesis, cell proliferation and anchorage-independent growth in soft-agar of the madtransfected cel1s were partially inhibited in comparison to control *** Cytometry analysis indicated that mad over-expression might block more transfectant cells at G0/G1 phase, resulting in the retardation of cell proliferation. RT-PCR detected a marked inhibition of the expression of cdc25A, an important regulator gene of G0/G1to S phase in cell cycle. It was also found that Mad protein overexpression could greatly suppress p53-mediated apoptosis in BEL-7404-M1 cells in the absence of ***, Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL7404 *** and regulation of cell growth and apoptosis

关键词： Mad c-Myc cell growth apoptosis human hepatoma cells

Tissue Distribution and Purification of Prophenoloxidase in Larvae of Asian Corn Borer, Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 360-364页

作者： Cong-Jing FENG1,2 and Wen-Jun FU1* 1institute of Plant Physiology and Ecology, shanghai institute for Biological sciences, the chinese academy of sciences, shanghai 200025, china 2The GraduateSchool of the chinese academy of sciences, Beijing 100039, china institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences the Chinese Academy of Sciences The Graduate School of the chinese academy of sciences

Using ammonium sulphate precipitation, Blue-Sepharose CL-6B, Phenyl-Sepharose CL-4B, prophenoloxidase (PPO) was isolated and purified from hemolymph of Ostrinia furnacalis larvae. This zy- mogen was a heterodimer, and composed of two subunits with the relative molecular mass ranging from 66.2 kD to 97.4 kD determined by SDS-PAGE. Western blotting and indirect immunofluorescence test showed that PPO was present in integument, hemolymph plasma and cell membrane of granular hemocytes and oenocytoids of O. furnacalis larvae.

关键词： Ostrinia furnacalis prophenoloxidase tissue distribution purification

A Novel Single-beam Magneto-optical Atom Trap

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chinese Journal of Lasers 2000年第3期9卷 64-71页

作者： GAO Wei-1 YIN Jianping- 1,2 WANG Yuzhu-1 ( -1 Laboratory for Quantum Optics, shanghai institute of Optics and Fine Mechanics, The chinese academy of sciences, shanghai 201800, china -2 Department of physics, Suzhou University, Suzhou 215006, Ch 中科院上海光学精密机械所量子光学开放实验室上海

A novel single-Gaussian-beam magneto-optical trap with a lens and spherical mirror is proposed. Our analysis and calculations show that it can trap as many as 10-6 atoms with a temperature of ～330 μK. Because of its extraordinary simplicity and much efficaciousness, the proposed single-beam trapping scheme will have many applications in the experiments of atom optics with cold and dense atoms.

关键词： laser cooling and trapping single-beam magneto-optical trap Gaussian laser beam