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Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid

Science china(Life Sciences) 2008年第3期51卷 280-285页

作者： LI Ang1,2, CHEN LiangLiang1,3, REN HaiYun3, WANG XueChen2, ZHANG HaiWen1,4 & HUANG Rong- Feng1,4 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, beijing 100081, china 2 national Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, china Agricultural University, beijing 100094, china 3 College of Life Sciences, beijing Normal University, beijing 100875, china 4 national key facility of crop gene resources and genetic improvement, beijing 100081, china Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China national Laboratory of Plant Physiology Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China College of Life Sciences Beijing Normal University Beijing 100875 China national key facility of crop gene resources genetic improvement Beijing 100081 China

In rice, the characterization of OsEBP-89 is inducible by various stress- or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further de- termine the crucial sequences responsible for MeJA induction, we generated a series of deletion pro- moters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is po- sitioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (?1127), which is distinct from the essential region containing ERE (?562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.

关键词： OsEBP-89 essential DNA region methyl jasmonic acid transient assay promoter tobacco leaves

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Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses

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Science china(Life Sciences) 2010年第11期53卷 1315-1321页

作者： GAO Fei1,2, SU Qi1, FAN YunLiu1& WANG Lei1 1Biotechnology Research Institute/national key facility for crop gene resources and genetic improvement, Chinese Academy of Agricultural Science, beijing 100081, china 2College of Life and Environmental Sciences, Minzu University of china, beijing 100081, china 1. Biotechnology Research Institute/national key facility for crop gene resources and genetic improvement Chinese Academy of Agricultural Science Beijing 100081 China2. College of Life and Environmental Sciences Minzu University of China Beijing 100081 China

The protein kinase AtMPK3,a component of the MAP kinase cascade,plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3 is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3 gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3 promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3 promoter, and introduced the intact and truncated promoter sequences fused to the β-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between-188 and-62 upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3 promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli.

关键词： Arabidopsis thaliana AtMPK3 promoter stress

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The optimization of soybean genetic transformation system mediated by Agrobacterium

The optimization of soybean genetic transformation system me...

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第十三届全国植物基因组学大会

作者： Bingfu Guo,Lijuan Qiu~* The national key facility for crop gene resources and genetic improvement(NFCRI), beijing 100081,china

Soybean[Glycine max(L).Merr]is one of the most important oil crops and the main source of edible vegetable oil and *** has played an important part in soybean breeding and more than 70%of soybean production in the wor...

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QTL for grain yield and yield parameters in winter wheat(Triticum aestivum L.)

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湖南农业大学学报（自然科学版） 2007年第S1期33卷 86-91页

作者： LIN Hai,GUO Hui-jun,ZHANG Xiu-ying,WANG Rui-xia,XIAO Shi-he(The national key facility for crop gene resources and genetic improvement,Institute of crop Science,12 Zhongguancun South Street,beijing,100081,P.R)

Field trials with a set of 108 doubled haploid lines(DHs) derived from a cross between the Chinese winter wheat ***9613 and H1488 were run at beijing(china).Phenotypic data were recorded for major agronomic yield traits,*** weight per ear,grain number per ear and thousand grain weight(Tgw) in two field trials at *** on the phenotypic data and a genetic map comprising 168 SSR markers,an analysis of quantitative trait loci(QTL) was carried out for yield and yield parameters using the composite interval mapping(CIM) approach.A total of 14 QTL were detected for these traits across two *** of these QTL located on chromosomes 1A and 2B,respectively,exhibited pleiotropic *** showing pleiotropic effects will be very useful for understanding the homeologous relationships of QTL and designing an appropriate marker-assisted selection programme by multi-trait selection in order to accumulate("pyramide") favorable alleles at different loci.

关键词： Doubled haploids Quantitative trait loci(QTL) Grain yield wheat(Triticum aestivum L.)

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MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

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Science Bulletin 2012年第Z2期 3804-3810页

作者： LUO ZhongQin,JIN LongGuo & QIU LiJuan * The national key facility for crop gene resources and genetic improvement(NFCRI),Institute of crop Science,Chinese Academy of Agricultural Sciences,beijing 100081,china

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5’RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词： soybean miR1511 miR1511 * GmRPL4a

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Novel alleles among soybean Bowman-Birk proteinase inhibitor gene families

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Science china(Life Sciences) 2008年第8期51卷 687-692页

作者： WANG YuePing1,2, CHEN XiongTing2 & QIU LiJuan1 1 national key facility for crop gene resources and genetic improvement, key Laboratory of crop Germplasm & Biotechnology(MOA)/Institute of crop Science, Chinese Academy of Agricultural Sciences, beijing 10008, china 2 Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, china 1. national key facility for crop gene resources and genetic improvement Key Laboratory of Crop Germplasm & Biotechnology (MOA)/Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 10008 China 2. Institute of Tropical Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China

Trypsin inhibitors have been found in various animals, plants and *** were two types of trypsin inhibitors in soybean including Bowman-Birk protease inhibitors(BBI) and Kunitz in-hibitors(KTI).The different BBI genes from wild soybean(***) and cultivated soybean(***) formed a multigene *** constructed a cDNA library of cultivar 'SuiNong 14' seed at the R7 growth stage using the SMART *** contigs or singletons were highly homologous to soy-bean protease *** of 5, 35, 8 and 9 were highly homologous to BBI family members BBI-A1, BBI-A2, BBI-C and BBI-D, *** analyses showed there were novel allelic varia-tions among the 4 BBI members in SuiNong *** on the comparison of soybean seed cDNA li-braries from different developmental stages, it was apparent that the expression of trypsin inhibitors increased during seed development in *** analysis of BBI gene sequences among dicotyledonous and monocotyledonous plants demonstrated that these genes shared a common pro-genitor.

关键词： soybean trypsin inhibitor Bowman-Birk proteinase inhibitor Kunitz trypsin inhibitor allelic variation

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Identification of genes contributing to quantitative disease resistance in rice

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Science china(Life Sciences) 2010年第11期53卷 1263-1273页

作者： KOU YanJun, LI XiangHua, XIAO JingHua & WANG ShiPing national key Laboratory of crop genetic improvement, national Center of Plant gene Research (Wuhan),Huazhong Agricultural University, Wuhan 430070, china 1. national key Laboratory of crop genetic improvement National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China

Despite the importance of quantitative disease resistance during a plant’s life, little is known about the molecular basis of this type of host-pathogen interaction, because most of the genes underlying resistance quantitative trait loci (QTLs) are unknown. To identify genes contributing to resistance QTLs in rice, we analyzed the colocalization of a set of characterized rice defense-responsive genes and resistance QTLs against different pathogens. We also examined the expression patterns of these genes in response to pathogen infection in the parents of the mapping populations, based on the strategy of validation and functional analysis of the QTLs. The results suggest that defense-responsive genes are important resources of resistance QTLs in rice. OsWRKY45-1 is the gene contributing to a major resistance ***,OsGH3-1,and OsGLP members on chromosome 8 contribute alone or collectively to different minor resistance QTLs. These genes function in a basal resistance pathway or in major disease resistance gene-mediated race-specific pathways.

关键词： quantitative disease resistance QTL defense-responsive gene rice

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Analysis of differential expression proteins of a dwarf soybean mutant by proteomlcs

Analysis of differential expression proteins of a dwarf soyb...

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第十三届全国植物基因组学大会

作者： Wei He,Lijuan Qiu~* The national key facility for crop gene resources and genetic improvement(NFCRI), Institute of crop Science,Chinese Academy of Agricultural Sciences,100081 beijing,china

Plant height is an important trait for crops and dwarf plant becomes one of important objectives of soybean ***808 is a dwarf mutant derived from Ethylmethylsulfonate(EMS) mutation of soybean cultivar *** this study d...

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Evolution of plant microRNA gene families

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Cell Research 2007年第3期17卷 212-218页

作者： Aili Li~1 Long Mao~(1,2) ~1Institute of crop Sciences,Chinese Academy of Agricultural Sciences and The national key facility for crop gene resources and genetic improvement (NFCRI),Beoing 100081,china ~2Department of Biological Sciences,Northern Illinois University,DeKalb,IL 60115,USA Institute of crop Sciences Chinese Academy of Agricultural Sciences and The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Beijing 100081 China.

MicroRNAs(miRNAs)are important post-transcriptional regulators of their target genes in plants and animals,miR-NAs are usually 20-24 nucleotides *** their unusually small sizes,the evolutionary history of miRNA genefamilies seems to be similar to their protein-coding *** contrast to the small but abundant miRNA familiesin the animal genomes,plants have fewer but larger miRNA gene *** of plant miRNA gene familiesare often highly similar,suggesting recent expansion via tandem gene duplication and segmental duplication *** many miRNA genes are conserved across plant species,the same gene family varies significantly in size andgenomic organization in different species,which may cause dosage effects and spatial and temporal differences in targetgene *** this review,we summarize the current progress in understanding the evolution of plant miRNA genefamilies.

关键词： microRNAs post-transcriptional regulation tandem duplication segmental duplication

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Proteomics Investigation of Wheat-Stripe Rust Fungus Interactions

Proteomics Investigation of Wheat-Stripe Rust Fungus Interac...

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第三届全国植物蛋白质组学大会

作者： Pan Yinghong Institute of crop Science,Chinese Academy of Agricultural Sciences(CAAS) /The national key facility for crop gene resources and genetic improvement(NFCRI)

Stripe rust of wheat is one of the most important diseases of wheat worldwide,but the mechanism of interaction between wheat and stripe rust fungus is *** using proteomics technologies,we identified some different pro...

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