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Representation and Adaptive Transformation of Reusable Software Components

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wuhan university Journal of Natural sciences 2001年第Z1期6卷 298-303页

作者： Xu Zheng quan, Palakiyem Wallah college of Computer science and technology,huazhong university of science and technology, wuhan 430074, china college of Computer science and technology Huazhong University of Science and Technology Wuhan 430074 China

The key ingredient for promoting software reuse in a repository base integrated development environment, is to provide support for the software developer who wants to search the repository to locate and retrieve suitable software components for reuse. This paper presents that reusable software components form a base to provide a flexible representation of component adaptability to any repository with a specific classification. As an alternative, it proposes to automatically identify and convert the component source code with all its information into a standard form, so that components can be transferred from one type of library to another.

关键词： reusable software component classification representation adaptability transfer

Deguelin regulates nuclear pore complex proteins Nup98 and Nup88 in U937 cells in vitro1

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Acta Pharmacologica Sinica 2005年第10期26卷 1265-1273页

作者： Hong-li LIU~2, Yan CHEN~(2,3), Guo-hui CUI~2, Qiu-ling WU~2, Jing HE~2, Wei-hua CHEN~2, Jian-feng ZHOU~4 ~2 department of Hematology, union hospital, tongji medical college, huazhong university of science and technology, wuhan 430022 ~4 department of Hematology, tongji hospital, tongji medical college, huazhong university of science and technology, wuhan 430030, china department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022

Aim: To investigate the anticancer effects and the molecular mechanisms of deguelin on human U937 leukemia cells, and to explore the underlying mechanism regulating nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro. Methods: The effects of deguelin on the growth of U937 cells were studied by MTT assay. The effect of deguelin on the cell cycle of U937 cells was studied by using a propidium iodide method. The localization of the nuclear pore complex proteins Nup98 and Nup88 was investigated by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 and Nup88 in U937 cells was investigated by using flow cytometry and Western blot. Results: The prolif eration of U937 cells was inhibited in the deguelin-treated group, with a 24-h IC50value of 21.61 nmol/L and a 36-h IC50value of 17.07 nmol/L. U937 cells treated with deguelin had reduced percentages of cells in the G0/G1phase, whereas cells accumulated in the S and G2/M phases. Nup88 and Nup98 were found on both the nuclar and cytoplasmic sides of the U937 cells by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 was upregulated and that of the Nup88 protein was downregulated in U937 cells treated with deguelin. Conclusion: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle such that cells are arrested at the S and G2/M phases, so that the proportion of cells in the G0/G1phase decreases. The antitumor effects of deguelin are related to upregulating the expression of Nup98 and downregulating the expression of Nup88 protein in U937 cells.

关键词： deguelin U937 cells nucleoporin Nup98 Nup88

Blockade of sonic hedgehog signal pathway enhances antiproliferative effect of EGFR inhibitor in pancreatic cancer cells

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Acta Pharmacologica Sinica 2007年第8期28卷 1224-1230页

作者： Wei-guo HU Tao LIU Jiong-xin XIONG Chun-you WANG~2 Pancreatic surgery Center,union hospital,tongji medical college,huazhong university of science and technology,wuhan 430022.china Pancreatic surgery Center Union HospitalTongji Medical CollegeHuazhongUniversity of Science and TechnologyWuhan 430022China

Aim:To investigate the expression of sonic hedgehog(SHH)and epidermal growthfactor receptor(EGFR)signal molecules in pancreatic cancer cells,and to assessthe inhibitory effects through the blockade of the SHH and EGFR signaling path-ways by cyclopamine and Iressa,***:The expression of SHHand EGFR in pancreatic cancer cell lines(PANC-I,SUIT-2,and ASPC-1)was de-tected by RT-PCR and Western blot *** treatment with different con-centrations of cyclopamine,alone or in combination with Iressa,the antiproliferativeeffect on pancreatic cancer cells was analyzed by methyl thiazolyl tetrazoliumassays.A flow cytometry analysis was used to detect the cellular cycle distribu-tion and apoptosis of pancreatic cancer ***:All of the 3 pancreaticcancer cell lines expressed SHH,Smoothened(SMO),and *** downregulate the expression of EGFR in all cell *** or Iressacould induce a growth inhibitory effect in a dose-dependent ***,the combined use of 2.5μmol/L cyclopamine and 1μmol/L iressa induced anenhanced inhibitory effect and a greater apoptosis rate than any agent *** of the cell population of the G0/G1and sub-G1phases was significantlyincreased along with the increasing dose of cyclopamine and/or ***:The blockade of the sonic hedgehog signal pathway enhances theantiproliferative effect of the EGFR inhibitor through the downregulation of itsexpression in pancreatic cancer *** simultaneous blockade of SHH andEGFR signaling represents possible targets of new treatment strategies for pan-creatic carcinoma.

关键词： pancreas cancer sonic hedgehog epidermal growth factor receptor inhibitor cyclopa-mine

Effect of telmisartan on expression of protein kinase C-α in kidneys of diabetic mice

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Acta Pharmacologica Sinica 2007年第6期28卷 829-838页

作者： Li-jun YAO~(2,4),Jian-qing WANG~2,Hong ZHAO~3,Jian-she LIU~2,An-guo DENG~2~2 department of Nephrology,union hospital,tongji medical college,huazhong university of science and technology wuhan 430030,china,~3 department of Trauma surgery,tongji hospital,tongji medical college,huazhong university of science and technology,430030wuhan.china department of Nephrology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China department of Trauma surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology 430030 Wuhan China

Aim:To investigate the effects of angiotensin receptor blocker (ARB) telmisartanon the expression and distribution of protein kinase C (PKC)-α in the kidneys ofdiabetic ***:Diabetic mice were induced with streptozotocin and agroup of them were randomly selected for treatment with *** 6weeks,the expression and localization of PKC-α in the renal cortex,and the outerand inner medulla were assessed by immunohistochemistry and semiquantitativeWestern *** addition,expressions of PKC-α,transforming growth factor-β1 (TGF-β1),and vascular endothelial growth factor (VEGF) in glomeruli weremeasured by semiquantitative ***:Diabetic and nor-mal mice showed similar distributions of PKC-α in the *** expression ofPKC-α was found in glomeruli,epithelial cells of proximal tubules,and medullary-collecting duct,while not in the medullary and cortical thick ascending limb,andwas different in the epithelial cells of proximal tubules of diabetic nephropathy(DN) mice,PKC-α was mostly translocated from the basement membrane to theapical membrane,whereas it was largely translocated from the apical membrane tothe basement membrane in epithelial cells of the inner medullary-collecting *** blotting detected increased expression of PKC-α in the renal cortex andouter medulla,but not in the inner medulla of DN *** expressions ofPKC-α,TGF-β1,and VEGF were shown in the glomeruli of DN mice,where PKC-αexhibited a correlation to VEGF,but no correlation to TGF-β*** telmisartanattenuated alterations of PKC-α as mentioned earlier in the DN ***:Our findings suggest that PKC-α may play a role in the pathogenesis of DN,andthat the nephroprotective effects of ARB telmisartan may be partly associatedwith its influence on PKC-α.

关键词： diabetic nephropathy protein kinases C-α telmisartan mouse kidney

Comparison of Arrhythmias among Different Left Ventricular Geometric Patterns in Essential Hypertension

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Journal of huazhong university of science and technology(medical sciences) 2001年第2期21卷 93-96页

作者：郑智周荣梁黔生 department of Emergency Medicine Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030

The differences of arrhythmias among distinct left ventricular geometric patterns in the patients with essential hypertension were studied. 179 patients with essential hypertension received 24 h dynamic ECG recording, ambulatory blood pressure monitoring, echocardiography examination, etc. According to the examinations, left ventricular geometric patterns and arrhythmias were identified. The comparison of morbidity of arrhythmias between the left ventricular remodeling group and the normal geometric pattern group was performed. The multiple stepwise regression analysis was carried out to identify the independent determinants of arrhythmias. After these predictors were controlled or adjusted, the severity of arrhythmias among different left ventricular geometric patterns was compared. It was found that the morbidity of atrial arrhythmia, ventricular arrhythmia and complex ventricular arrhythmias in the left ventricular remodeling group was significantly higher than in the normal geometric pattern group respectively. There were many independent factors influencing on arrhythmias in essential hypertension. Of all these factors, some indices of left ventricular anatomic structure, grade of hypertension, left atrial inner dimension, E/A, diastolic blood pressure load value at night and day average heart rate and so on were very important. After the above mentioned factors were adjusted, the differences of the orders of arrhythmias between partial geometric patterns were reserved, which resulted from the differences of the geometric patterns. Many factors contributed to arrhythmias of essential hypertension, such as grade of hypertension, LVMI, LA, PWT and so on. The severity of arrhythmias was different in different left ventricular geometric patterns.

关键词： essential hypertension left ventricular remodeling arrhythmia echocardiography

Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion

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Acta Pharmacologica Sinica 2004年第5期25卷 81-87页

作者： Jun-qiang SI2, Zhi-qin ZHANG, Chun-xia LI, Li-feng WANG, Yun-lei YANG3, Zhi-wang LI3department of Physiology, medical college Shihezi university, Shihezi 832002, china3 Now in tongji medical college, huazhong university of Sci-ence and technology, wuhan 430030, china. department of Physiology Medical College Shihezi University

AIM: To explore the modulatory effect of substance P (SP) on GABA-activated current of dorsal root ganglion(DRG) neurons in rat. METHODS: The whole-cell patch-clamp technique was used to record SP- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons. Drugs were applied by rapid solutionexchange. RESULTS: Application of SP (28/41, 68.5 %) and GABA (36/41, 88.2 %) could induce concentration-dependent inward current in some cells. SP-(10 mol/L) and GABA (100 mol/L)-activated inward currents were(24483) pA (n=9) and (1.80.5) nA (n=13), respectively. The majority of GABA-activated current had obviousthree processes, the peak value (Ip), the steady state (Iss) and the desensitization (Id). The desensitization of GABA-activated current was a biphasic process, including fast and slow desensitization. However, pre-application of SP(0.0011 mol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects were concentration-dependent. The inhibitory effect of SP on the peakvalue of GABA-activated current was more than the steady state of GABA-activated current. The inhibition ofGABA-activated current by SP (0.1 mol/L) was related to the time after application of SP, the inhibition of GABA-activated currents by SP reached the peak at about 4 min (49.8 %7.2 %, n=7, P<0.01) and took about 12 min toget a full recovery. The inhibition of GABA-activated currents by SP was almost completely removed after block-ade of PKC by H-7 with the re-patch clamp. CONCLUSION: Pre-application of SP exerts a more strong inhibitoryeffect on the peak value of GABA-activated current than the steady state of GABA-activated current.

关键词： patch-clamp techniques spinal ganglia substance P GABA protein kinase C

SOLUTION PROPERTIES OF ANTITUMOR CARBOXYMETHYLATED DERIVATIVES OF α-(1→3)-D-GLUCAN FROM GANODERMA LUCIDUM

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Chinese Journal of Polymer science 2001年第3期19卷 283-289页

作者： Li-na Zhang Mei Zhang Jing-hua Chen Fan-bo Zeng department of Chemistry wuhan university, wuhan 430072, china Pharmacy college, tongji medical university, wuhan 430072, china wuhan Univ Dept Chem Wuhan 430072 Peoples R China tongji Med Univ Coll Pharm Wuhan 430072 Peoples R China

Fractions of a water insoluble α-(1→3)-D-glucan (GL) extracted from Ganoderma lucidum were carboxy-methylated (CM) to obtain water-soluble carboxymethylated derivatives (CM-GL) having a degree of substitution (DS) of0.38～0.51. Weight-average molecular weight M_w and intrinsic viscosity [η] of the samples CM-GL were measured by gelpermeation chromatography combined with laser light scattering (GPC-LLS) and viscometry. The CM-GL exhibits a stifferchain in aqueous solution at 25℃ than the original glucan, The antitumor activities against Ehrlich ascites carcinoma (EAC,5×10~6) of the carboxymethylated derivatives from the α-glucan and curdlan, a β-glucan, are significantly higher than thoseof the original glucans. The effects of the relatively low molecular weight, expanded chains and better water-solubility of theCM-GL on the enhancement of antitumor activity could not be neglected. The chain stiffness decreased speedily withincrease of temperature from 40 to 60℃ or NaOH concentration from 0.1 to 0.4 in the solution, respectively, and the changeof the chain stiffness is reversible.

关键词： Solution properties Antitumor activity Carboxymethylated derivative α-D-glucan Ganoderma lucidum

Hepatectomy for patients with huge primary liver cancer in Hubei Province of china

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Hepatobiliary & Pancreatic Diseases International 2002年第1期1卷 46-51页

作者： Xiao-Ping Chen Bi-Xiang Zhang Zai-De Wu Fa-Zu Qiu From the Hepatic surgery Center, tongji hospital, tongji medical college, huazhong university of science and technology, wuhan 430030, china Hepatic surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China.

Objective: To discuss the safety and feasibility of hep-atectomy for huge primary liver cancer (PLC).Methods: The effect of resection of huge PLC was ex-amined retrospectively. Some problems in resection ofhuge PLC were ***: Of 375 patients with huge PLC undergoinghepatectomy, 11 (2.9%) died in one month after op-eration. The 1-, 2-, 3-, 5- and 10-year survivalrates of the patients were 63.3%, 45.6%, 34.7%,16.5% and 1.8%, respectively. The effect of prolong-ing survival time was ***: Hepatectomy for huge PLC is safe, feasi-ble, and effective.

关键词： primary liver cancer hepatectomy

Relationship between the Abnormal Expression of FasL on Human First Trimester Trophoblast and Spontaneous Abortion

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Journal of huazhong university of science and technology(medical sciences) 2003年第1期23卷 57-59页

作者：邱红玉孙永玉 departmentofObstetricsandGynecology XieheHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology

In order to study the molecular immune-pathological mechanism of spontaneous abortion (SA), immunohistochemistry techniques were used to detect the FasL expression of first trimester trophoblast in the SA patients and normal controls. High precise color-image measure system for immuno-histochemistry (HPIS) was used to determine the quantity of FasL expression. The results showed that the scale and intensity of FasL expression on the trophoblasts in SA group were significantly lower than in the control group. It is indicated that abnormal expression of FasL on trophoblasts, which damages the immunological tolerance between mother and fetus, may be one of the important mechanisms of development of SA. To induce the expression of FasL or to regulate the immunological tolerance will be a new way to treat SA.

关键词： FasL spontaneous abortion immunology