Effect of telmisartan on expression of protein kinase C-α in kidneys of diabetic mice

作     者：Li-jun YAO~(2,4),Jian-qing WANG~2,Hong ZHAO~3,Jian-she LIU~2,An-guo DENG~2~2 Department of Nephrology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology Wuhan 430030,China,~3 Department of Trauma Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,430030Wuhan.China 

作者机构：Department of Nephrology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Trauma Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2007年第28卷第6期

页      面：829-838页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100602[医学-中西医结合临床] 10[医学] 

基　　金：funds from Union Hospital Tongji Medical College Huazhong University of Science and Technology(№ 2005-21) 

主　　题：diabetic nephropathy protein kinases C-α telmisartan mouse kidney 

摘      要：Aim:To investigate the effects of angiotensin receptor blocker (ARB) telmisartanon the expression and distribution of protein kinase C (PKC)-α in the kidneys ofdiabetic ***:Diabetic mice were induced with streptozotocin and agroup of them were randomly selected for treatment with *** 6weeks,the expression and localization of PKC-α in the renal cortex,and the outerand inner medulla were assessed by immunohistochemistry and semiquantitativeWestern *** addition,expressions of PKC-α,transforming growth factor-β1 (TGF-β1),and vascular endothelial growth factor (VEGF) in glomeruli weremeasured by semiquantitative ***:Diabetic and nor-mal mice showed similar distributions of PKC-α in the *** expression ofPKC-α was found in glomeruli,epithelial cells of proximal tubules,and medullary-collecting duct,while not in the medullary and cortical thick ascending limb,andwas different in the epithelial cells of proximal tubules of diabetic nephropathy(DN) mice,PKC-α was mostly translocated from the basement membrane to theapical membrane,whereas it was largely translocated from the apical membrane tothe basement membrane in epithelial cells of the inner medullary-collecting *** blotting detected increased expression of PKC-α in the renal cortex andouter medulla,but not in the inner medulla of DN *** expressions ofPKC-α,TGF-β1,and VEGF were shown in the glomeruli of DN mice,where PKC-αexhibited a correlation to VEGF,but no correlation to TGF-β*** telmisartanattenuated alterations of PKC-α as mentioned earlier in the DN ***:Our findings suggest that PKC-α may play a role in the pathogenesis of DN,andthat the nephroprotective effects of ARB telmisartan may be partly associatedwith its influence on PKC-α.