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In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of ethyl acetate extract of Mallotus repandus (Willd.) Muell. stem in rat model

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Journal of Coastal Life Medicine 2014年第9期 721-726页

作者： md.Rakib Hasan Nizam Uddin md.Monir Hossain md.Mahadi Hasan md.Emtiaz Yousuf Swagata Sarker Lopa Tasmina Rahman Mohammad Shahabuddin Kabir Choudhuri Department of Pharmacy Jahangirnagar UniversitySavarDhaka-1342Bangladesh

Objective:To investigate the therapeutic effects of ethyl acetate extract of Mallotus repandus stem in α-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Ethyl acetate extract of Mallotus repandus stem was tested for the presence of phytochemical constituents,α-amylase inhibitory activity and hypoglycemic effect in normal rats and glucose induced hyperglycemic ***:Presence of different types of phytochemicals was identified in the *** extract has moderate α-amylase inhibitory activity[IC_(50)=(2.038±0.033)mg/mL]as compared to *** does 1000 mg/kg significantly reduced(Pblood glucose level in normal *** oral glucose tolerance test,both 1000 and 2000 mg/kg doses showed good hypoglycemic activity(P3,48)value=***:These findings suggest that this plant may be a potential source for the development of new oral hypoglycemic agent.

关键词： Mallotus repandus α-Amylase Diabetes mellitus Hypoglycemic Oral glucose tolerance test

Hepatoprotective effects of Nigella sativa seed extract against acetaminophen-induced oxidative stress

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Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 217-222页

作者： Gareeballa Osman Adam md.Mahbubur Rahman Sei-Jin Lee Gi-Beum Kim Hyung-Sub Kang Jin-Shang Kim Shang-Jin Kim Department of Veterinary Medicine and Surgery College of Veterinary Medicine.Sudan University of Science and Technology Department of Veterinary Pharmacology and Toxicology College of Veterinary Medicine.Chonbuk National University Korea Basic Science institute Jeonju Center

Objective:To investigate the protective effects of Nigella sativa seed extract(NSSE) against acetaminophen(APAP)-induced hepaloloxicity in TIB-73 cells and ***:Toxicity in TIB-73 cells was induced with 10 μmol/L APAP and the protective effects of NSSE were evaluated at 25.50.75,100 μg/*** in rim examination,a total of 30 rals were equally divided into five experimental groups:normal control(vehicle),APAP(800 mg/kg body weight single IP injection) as a hepatotoxic control,and three APAP and NS pretreated(2 weeks) groups(APAP+NSSE 100 mg:APAP+NSSE 300 mg and APAP+NSSK 900 mg/kg).Results:TIB-73 cell viability was drastically decreased by(49.0±l.9)%after the 10 μmol/L APAP treatment,which also increased reactive oxygen species ***-treatment with NSSE at 25.50.75,and 100 μg/mL significantly improved cell viability and suppressed reactive oxygen species *** viro the APAP induced alterations in blood lactate levels,pH,anionic gap,and ion levels(HCO_3^-,Mg^(2+) and K^+),which tended to normalize with the NSSE *** NSSE also significantly decreased elevated serum levels of alanine aminotransferase,aspartate aminotransferase,lactate dehydrogenase,and alkaline phosphatase induced by APAP,which correlated with decreased levels of hepatic lipid peroxidation(nialondialdehyde),increased superoxide dismutase levels,and reduced glutathione *** hepatic histology was also found in the treatment groups other than APAP ***:The in vitro and in vim findings of this study demonstrated that the NSSE has protective effects against APAP-induced hepalotoxicity and metabolic disturbances by improving antioxidant activities and suppressing both lipid peroxidation and ROS generation.

关键词： Nigella sativa Acetaminophen Antioxidants Oxidative stress TIB-73 cells Rat

CLONAL PROLIFERATION AND LONG-TERM CULTURE OF MALIGNANT LYMPHOMA CELLS UNDER SERUM-FREE CULTURE CONDITIONS

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Chinese Journal of Cancer research 1990年第4期2卷 30-33页

作者：戴育成 Wang XH Wang C Jamal N Biondi A Minden md Messner HA Jiangxi institute of Medical Sciences Nanchang Ontario Cancer institute TorontoOntario.Canada

We developed a serum-free culture system that promoted the growth of B cell colonies in peripheral blood, bone marrow, lymph nodes and cerebrospinal fluid (CSF) from 7 out of 8 patients with non-Hodgkin's lymphomas of B cell type. The culture cells were pretreated with or without galactose oxi-dase (GO) prior to plating. Colony growth was best supported with BCGF. A moderate increment was observed with rIL-3, as well as rIL-1β and even to a lesser degree, by rlL-2, while B cell stimulating factor-2 (rBCSF-2) and rlL-1β did not show significant activity. rGM-CSF and rG-CSF had little effect, while rM-CSF enhanced the formation of lymphoma colonies. The cells from different patients had different requirements for Staphylococcus aureus protein A and GO pretreatment. It reflected the differences in activation and differentiation status and surface properties of lymphoma cells from different patients. The cells from CSF of one patient were successfully maintained in serum-free culture medium supplemented with 10% BCGF or 5% PHA-LCM for more than 4 months. The long-term culture cells were EBV negative, phenotypically consistent with B cells and gene rearrangements for JH, Kappa and myc. This serum-free culture system allowed extensive analysis of the growth requirements for clonogenic precursors.

关键词： NHL CLONAL PROLIFERATION AND LONG-TERM CULTURE OF MALIGNANT LYMPHOMA CELLS UNDER SERUM-FREE CULTURE CONDITIONS CSF

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小青龙汤联合西药内服治疗慢性阻塞性肺疾病发作期临床疗效的系统评价

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世界科学技术-中医药现代化 2013年第3期15卷 599-607页

作者：高振刘莹莹哈木拉提·吾甫尔徐丹荆晶李风森新疆医科大学附属中医医院/新疆·国家中医临床研究基地新疆医科大学中医学院

目的:评价小青龙汤联合西药内服治疗慢性阻塞性肺病发作期的临床疗效。方法:按Cochrane系统评价方法,计算机检索CNKI、VIP、CBM、万方等数据库,检索时间从建库至2012年8月3日,并手工检索相关文献,查找小青龙汤联合治疗慢性阻塞性肺病发... 详细信息

目的:评价小青龙汤联合西药内服治疗慢性阻塞性肺病发作期的临床疗效。方法:按Cochrane系统评价方法,计算机检索CNKI、VIP、CBM、万方等数据库,检索时间从建库至2012年8月3日,并手工检索相关文献,查找小青龙汤联合治疗慢性阻塞性肺病发作期的随机和半随机对照试验。由两位研究者按照纳入排除标准筛选文献,评价质量,并提取资料,采用RevMan 5.0软件进行Meta分析。结果:纳入17个随机对照试验,共1 173例患者,其中试验组608例,对照组565例。Meta分析结果显示:(1)临床疗效:与西药组相比,小青龙汤联合西药内服有统计学意义[md=3.91,95%CI(2.50,6.12),Pmd=3.48,95%CI(1.45,8.32),P=0.01];(2)血气分析:与西药组相比,氧分压(PaO2)测量,小青龙汤联合西药内服有统计学意义[md=7.55,95%CI(1.41,13.68),P=0.05],二氧化碳分压(PaCO2)测量,小青龙汤联合西药内服有统计学意义[md=-7.11,95%CI(-9.89,-4.33),Pmd=-6.66,95%CI(-8.79,-4.54),P3)FEV1:与西药组相比,小青龙汤联合西药内服组有统计学意义[md=6.97,95%CI(3.60,10.34),P<0.01]。结论:小青龙汤内服联合西医手段治疗慢性阻塞性肺疾病可一定程度提高临床疗效。

关键词：慢性阻塞性肺病中医小青龙汤系统评价 Meta分析

合并糖代谢异常的冠心病患者血浆脂联素水平变化及其临床意义

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中南大学学报（医学版） 2012年第2期37卷 179-184页

作者：夏珂郭兰燕赵震宇 Ali Sheikh md Sayed 李非杨天伦中南大学湘雅医院心内科长沙410008 中南大学临床药理研究所长沙410008

目的:检测脂联素(adiponectin,ADPN)在合并糖代谢异常的冠心病患者血浆中浓度的变化,探讨其临床意义。方法:收集2009年8月至2010年4月在湘雅医院心内科经冠状动脉造影(coronary angiography,CAG)检查证实为冠心病的患者87名,根据糖代谢... 详细信息

目的:检测脂联素(adiponectin,ADPN)在合并糖代谢异常的冠心病患者血浆中浓度的变化,探讨其临床意义。方法:收集2009年8月至2010年4月在湘雅医院心内科经冠状动脉造影(coronary angiography,CAG)检查证实为冠心病的患者87名,根据糖代谢情况分为单纯冠心病组(coronary heart disease group,CHD组,n=31)、冠心病合并糖耐量异常组(CHD combine with impaired glucose tolerance group,CHD+IGT组,n=28)和冠心病合并糖尿病组(CHDcombine with diabetes mellitus group,CHD+DM组,n=28),另选健康体检者31例为正常对照组(normal control group,NC组)。采用酶联免疫吸附法检测血浆ADPN水平;为所有受试对象测身高、体质量、腰围、血压;测空腹血糖、胰岛素、血脂、高敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)、游离脂肪酸(free fatty acid,FFA)和肝、肾功能等,计算体质量指数(body mass index,BMI)和胰岛素抵抗指数(homeostasis model assessment for insulin resistance,HOMA-IR)。结果:1)CHD组、CHD+IGT组、CHD+DM组患者血浆ADPN水平均低于NC组(P3)ADPN与HDL-C呈正相关(r=0.483,P<0.01),与hs-CRP和冠脉Gensini积分呈负相关(r=–0.489,P<0.05;r=–0.252,P<0.05)。结论:冠心病患者血浆ADPN浓度降低;合并糖代谢异常的冠心病患者血浆ADPN浓度更低,且随着糖代谢异常程度加重降低更显著;冠心病与糖代谢异常是影响血浆ADPN浓度改变的两个重要因素,血浆ADPN水平显著降低反映了上述两种疾病状态的叠加作用。ADPN联合HDL-C,hs-CRP,Gensini积分等血清学指标可为判断合并糖代谢异常的冠心病患者的疾病严重程度提供一定参考。

关键词：脂联素冠心病糖代谢异常糖耐量异常糖尿病

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血小板减少时纤维蛋白原水平对血栓弹力测定参数的影响

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麻醉与镇痛 2011年第3期7卷 39-47页

作者： Thomas Lang md Kai Johanning, md Helfried Metzler, md Siegfried Piepenbrock, md Cristina Solomon, md NielsRahe-Meyer, md, PhD Kenichi A. Tanaka, md, MSc 杨静(译) Department of Anesthesiology Medical School Hannover Germany Emory University School of Medicine Atlanta GA Werlhof Institut Hannover GermanyEmory University School of Medicine Atlanta GA Department of Anesthesiology University Medical School Graz Austria Emory University School of Medicine Atlanta GA Department of Anesthesiology Emory University School of Medicine Atlanta GA 不详

背景纤维蛋白原及纤维蛋白与血小板的结合，在正常的止血过程中发挥着重要作用。血小板一纤维蛋白相互作用的程度可通过旋转式血栓弹力测定法（ROTEM）测量血凝块粘弹性的强度进行评估。在本试验中，我们研究了纤维蛋白原浓度对ROTEM测... 详细信息

背景纤维蛋白原及纤维蛋白与血小板的结合，在正常的止血过程中发挥着重要作用。血小板一纤维蛋白相互作用的程度可通过旋转式血栓弹力测定法（ROTEM）测量血凝块粘弹性的强度进行评估。在本试验中，我们研究了纤维蛋白原浓度对ROTEM测得整体血凝强度的影响及其发挥的作用。方法采集健康志愿者的血样作为研究样品。通过ROTEM检测一系列用同源血浆调配为含不同血小板数量的富含血小板血浆（PRP）的最大凝固程度（MCE），以此评估血小板数量对血凝强度的影响。经调配后PRPs的血小板数量分别为10×10^3mm^-3、50×10^3mm^-3和100×10^3mm^-3，并各加入浓度为550和780mg／dl的纤维蛋白原浓缩液。纤维蛋白的聚合作用可不依赖于血小板的附着对血凝强度产生影响，细胞松弛素D－修正的血栓弹力测定法（FIBTEM）可对以上影响进行分析。此外，本研究还回顾性分析了两组血小板减少患者的血凝强度（MCE）。结果血小板低于100×10^3mm^-3时血凝强度（MCE）有所降低，而当血小板数量为400×10^3mm^-3时MCE上升达峰值并延伸为一平台。PRP中渐增的纤维蛋白原浓度以浓度依赖的方式逐渐增加血凝强度，即使在血小板数量较低时（10×10^3mm^-3）仍有同样表现。904例血小板减少患者的资料分析也证实血凝强度和血浆纤维蛋白原水平之间存在正相关。结论这些体外研究和临床资料的ROTEM分析结果提示，血凝强度以纤维蛋白原浓度依赖的方式增加，而且不依赖血小板的数量。血小板减少时维持纤维蛋白原的浓度至关重要。外源性激活（EXTEM）和FIBTEM对于指导纤维蛋白原的补充治疗具有一定应用价值。

关键词：纤维蛋白原水平血小板减少血小板数量测定弹力血栓纤维蛋白原浓度富含血小板血浆

Hyponatremic hypertensive syndrome-a retrospective cohort study

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World Journal of Nephrology 2017年第1期6卷 41-44页

作者： Devdeep Mukherjee Rajiv Sinha md Shakil Akhtar Agni Sekhar Saha Department of Pediatric Medicine Institute of Child HealthKolkata 700017India Fortis Hospitals Kolkata 700017India

AIM To ascertain the frequency of hyponatremic hypertensive syndrome （HHS） in a cohort of children with hypertensive emergency in a tertiary pediatric *** A retrospective review was undertaken among children with hypertensive emergency admitted in our tertiary children hospital between June 2014 and December 2015 with an aim to identify any children with HHS. Three children with HHS were identifed during this *** The 3 patients with HHS presented with hypertensive emergency. They were initially managed with Labetalol infusion and thereafter switched to oral anti-hypertensives （combination of Nifedipine sustained release, Hydralazine and Beta Blocker）. All 3 were diagnosed to have unilateral renal artery stenosis. One child was lost to follow up, whereas the other 2 underwent renal angioplasty which was followed with normalization of blood *** Despite activation of renin angiotensin axis secondary to renal artery stenosis, these groups of children have significant hyponatremia. Renal re-vascularisation pro-duces excellent results in most of them.

关键词： Hypertension Hyponatremia Renovascular Encephalopathy Paediatric

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针灸刺激和光疗的微循环反应

麻醉与镇痛 2010年第3期6卷 95-100页

作者： Makiko Komori, md Katsumi Takada, md Yasuko Tomizawa, md Keiko Nishiyama, md Izumi Kondo, md Miwako Kawamata, md Makoto Ozaki, md 谢珺田(译) 宋文阁(校) Department of Anesthesiology Medical Center East Tokyo Women's Medical University Nishiogu Arakawa-ku Tokyo Japan Departments of Anesthesiology Cardiovascular Surgery School of Medicine Tokyo Women's Medical University I(awadacho Shinjuku-ku Tokyo Japan 不详

背景据报道针灸和光照疗法有镇痛和改善微循环的作用。然而，尚未见直接研究周围血管变化的报道，包括客观评估或定量研究。我们以直视下观察小动脉血流量对针灸刺激和光疗的反应对这些方法的效果进行评估。方法用40只家兔，将兔耳室置... 详细信息

背景据报道针灸和光照疗法有镇痛和改善微循环的作用。然而，尚未见直接研究周围血管变化的报道，包括客观评估或定量研究。我们以直视下观察小动脉血流量对针灸刺激和光疗的反应对这些方法的效果进行评估。方法用40只家兔，将兔耳室置入兔耳廓内，固定于解剖显微镜下。以显微镜摄像头选定小动脉进行观察。静脉注射戊巴比妥，行气管插管并保持自主呼吸。将家兔随机分为针灸组（n=10）、近红外线灯照射组（即灯照组，n=10）、低功率近红外激光照射组（即激光组，n=10）和无照射组（即对照组，n=10）。针灸组将针灸针于耳廓留置20分钟；灯照组以1540mW红外线灯照射1秒继之停顿4秒，再重复操作；激光组以60mW激光连续照射。灯照组和激光组，均用接触探头照射耳廓（取与针灸相同部位）10分钟。针灸或照射之前和之后60分钟测定小动脉直径和血流速度。以血流速度乘以血管横截面积计算出血流速率。结果与治疗前（100％）比较，针灸组小动脉直径增大至131％±14％（P〈0．005），灯照组增大至129％±19％（P〈0．005），激光组增大至128％±11％（P〈0．005）。针灸刺激结束后20分钟，光照和激光照射结束后10分钟达到最大值。这3组与对照组比较后显示小动脉直径显著增加（P〈0．005）。血流速度和血流量变化与小动脉直径变化相似。治疗效果在刺激和照射结束后持续40～50分钟。结论针灸刺激和光疗直接增加外周小动脉直径并加快血流速度。全身和局部副作用最小的针灸刺激和光照治疗能改善微循环，可成为外周循环不良所致疾病的有效辅助治疗方法。

关键词：循环反应灸刺激光疗红外线灯照射激光照射动脉直径改善微循环血流速度

应用全血阻抗法和传统透光聚集度评估体外循环所致血小板聚集功能改变的试验性研究

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麻醉与镇痛 2011年第3期7卷 48-56页

作者： Corinna Velik-Salchner, md Stephan Maier, md Petra Innerhofer, md Christian Kolbitsch, md Werner Streif, md Markus Mittermayr, md Michael Praxmarer, MSc Dietmar Fries, md 冯雪辛(译) Departments of Anesthesiology and Intensive Care Medicine Innsbrudk Medical University Innsbruck Austria Pediatrics Innsbruck Medical University Innsbrudk Medical University Innsbruck Austria Assign Data Management and biostatistics lnnsbruck Austria Innsbrudk Medical University Innsbruck Austria Department of General and Surgical Critical Care Medicine Innsbrudk Medical University Innsbruck Austria 不详

背景本研究分别采用全血阻抗法与传统透光聚集度（LTA）测定血小板聚集率，以探讨抗血小板治疗药物是否会降低心脏手术或体外循环（CPB）后的血小板聚集功能。方法选择70例拟行择期冠状动脉旁路移植术的患者，分别于麻醉诱导前、诱导中... 详细信息

背景本研究分别采用全血阻抗法与传统透光聚集度（LTA）测定血小板聚集率，以探讨抗血小板治疗药物是否会降低心脏手术或体外循环（CPB）后的血小板聚集功能。方法选择70例拟行择期冠状动脉旁路移植术的患者，分别于麻醉诱导前、诱导中和肝素化后15分钟及3小时采取动脉血，应用Multiplate（M）测定随时间变化的电阻值作为聚集单位，同时使用以胶原（COL）、二磷酸腺苷（ADP）或花生四烯酸（AA）为激活剂的LTA（％聚集率）法进行检测。A组（n=48）为对照组，手术前停用抗血小板药物至少7天，B组（n=11）和C组（n=11）患者分别服用阿司匹林100mg／d或阿司匹林100mg／d与氯吡格雷75mg／d（双重抗血小板治疗）至手术前一天。结果未行抗血小板治疗的患者，给予鱼精蛋白15分钟及3小时后，应用3种激活剂及两种聚集方法均可观察到血小板聚集率明显降低。单纯使用阿司匹林的患者，LTA—COL、LTA—ADP及M—ADP随时间发生显著改变；接受双重抗血小板治疗的患者，两种方法的ADP检测均提示给予鱼精蛋白15分钟后血小板聚集显著减弱。通过计算受试者工作特征曲线（receiver-operatingcharacteristiccurves，ROC曲线）下面积来区分抗血小板药物，LTA-COL可以在CPB后15分钟和3小时区分出对照组与阿司匹林组或双重抗血小板治疗组，M—ADP则可在鱼精蛋白拮抗后3小时区分对照组和双重抗血小板治疗组。结论对于未经抗血小板治疗的患者，全血电阻抗法及使用了常用激活剂的传统LTA法均可检测出CPB所致的血小板聚集功能改变。而对于接受了抗血小板治疗的患者，ADP激活的抗血小板检测方法能更好地检测出CPB所致的血小板聚集功能减退。

关键词：血小板聚集功能电阻抗法体外循环试验性研究全血应用抗血小板药物抗血小板治疗