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Co-expression of CXCR4 and CD133 proteins is associated with poor prognosis in StageⅡ-Ⅲcolon cancer patients

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Co-expression of CXCR4 and CD133 proteins is associated with...

2012年全国肿瘤分子标志学术大会与国际肿瘤转化医学论坛暨第七届中国中青年肿瘤专家论坛

作者： Nian-hua Zhang~(1,2#),Jie Li~(3#),Yin Li~4,Xin-tao Zhang~5,Wen-ting Liao~(6,7,8),Jun-yi Zhang~1,Rong Li~1,Rong-cheng Luo~(1*) 1,Department of Oncology,Nanfang Hospital,Southern Medical university,guangzhou 510515,china 2,Department of Oncology,guangdong Second Provincial traditional chinese medicine Hospital,guangzhou 510095,china 3,Department of Vascular Surgery, The First Affiliated Hospital,Sun Yat-Sen university,guangzhou 510080,china 4, Department of Gastrointestinal Surgery,The First Affiliated Hospital,Sun Yat-Sen university,guangzhou 510080,china 5,Department of Sports medicine,Peking university Shenzhen Hospital,Shenzhen 518036,china 6,Department of Pathology,School of Basic Medical Sciences,Southern Medical university,guangzhou 510515,china 7,Department of Pathology,Nanfang Hospital,Southern Medical university,guangzhou 510515,china 8, guangdong Provincial Key laboratory of Molecular Tumour Pathology,guangzhou 510515, china

Although CXCR4 and CD133 have been implicated in the metastatic process of malignant tumors,the clinicopathological significance of their expression in human colon cancer is not fully *** present study was aimed to examine the expression of CXCR4 and CD133 protein in stageⅡorⅢcolon cancer and related lymph nodes,and to investigate the clinical and prognostic significance in colon cancer. Immunohistochemistry was performed to examine CXCR4 and CD133 protein expression in paraffin-embedded stageⅡorⅢprimary colon cancer tissues and matched lymph nodes. The correlation between the two proteins expression and clinicopathological parameters and the patients’ 5-year survival was *** have found that CXCR4 expression was detected in 74 of the 125 tumors(59.2%),and CD133 expression was detected in 45 of the 125 tumors(36.0%).Upon combining the expression of CXCR4 and CD133,co-expression (both CXCR4 and CD133 were positve) was detected in 29 of the 125 tumors(23.2%).When compared with the other combinations,co-expression of CXCR4 and CD133 proteins was significantly associated with the AJCC stage(P = 0.029) and the lymph node status(P = 0.020).Log-rank statistics showed that the AJCC stage(P = 0.014),lymph node status(P = 0.011),CXCR4 expression(P = 0.023),CD133 expression(P = 0.034),and co-expression of CXCR4 and CD133 proteins(P = 0.003) were significant prognostic indicators for overall patients *** have shown that co-expression of CXCR4 and CD133 proteins is a risk factor for worse overall survival in stageⅡorⅢcolon cancer patients, indicating that co-expression of CXCR4 and CD133 proteins might contribute to the progression of colon cancer.

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温针灸对冠心病血瘀证患者血脂及血液流变学的干预研究

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上海针灸杂志 2011年第10期30卷 656-658页

作者：老锦雄潘清洁广州中医药大学附属佛山市中医院针灸科广东528000 广州中医药大学 2008级硕士生广东510405

目的观察背俞穴温针灸治疗对血脂、血液流变学的影响,了解其对冠心病患者的治疗作用和机理。方法将100例非急性期冠心病患者随机分为治疗组和对照组,对照组采用基础药物治疗,治疗组在基础药物治疗基础上加用温针治疗,两组治疗前后均行... 详细信息

目的观察背俞穴温针灸治疗对血脂、血液流变学的影响,了解其对冠心病患者的治疗作用和机理。方法将100例非急性期冠心病患者随机分为治疗组和对照组,对照组采用基础药物治疗,治疗组在基础药物治疗基础上加用温针治疗,两组治疗前后均行血脂、血液流变学指标检查。结果治疗组治疗后血脂、血液流变学指标与治疗前相比差异有统计学意义(P<0.01),两组治疗后比较差异有统计学意义(P<0.01)。结论背俞穴温针灸对非急性期冠心病患者的血脂、血液流变学作用明显,可干预动脉粥样硬化斑块的形成与进展,是预防和治疗心血管疾病的有效方法。

关键词：穴,背俞温针疗法冠心病动脉粥样硬化血液流变学血脂

Electro-acupuncture for STAT3 expression and nuclear translocation in hippocampal tissues of rats following cerebral ischemia/reperfusion

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Neural Regeneration Research 2006年第8期1卷 717-720页

作者： Lihong Kong1, Xiaoling Zeng1, Guojie Sun1, Shenghong Liu2 1Staff Room of Acupuncture and Moxibustion, Department of Acupuncture and Bone Injury, Hubei College of traditional chinese medicine, Wuhan 430061, Hubei province, china 2Tongji Medical College, Huazhong university of Science and Technology, Wuhan 430061, Hubei province, china Staff Room of Acupuncture and Moxibustion Department of Acupuncture and Bone Injury Hubei College of Tradiational Chinese Medicine Wuhan 430061 Hubei Province China Tongji Medical College Huazhong University of Science and Technology Wuhan 430061 Hubei Province China

BACKGROUND: It has been found in recent years that STAT3 widely distributes in nervous system, including hippocampal CA1-3 region, dentate gyrus and cerebral neocortex, etc. Ischemic brain injury can cause the release of some cytokines and growth factors, while electro-acupuncture may have multi-level, multi-channel and multi-target protective and interventional effects on ischemic brain injury. OBJECTIVE: To observe the effects of electro-acupuncture on STAT3 expression and nuclear translocation in hippocampal CA1 region of rat models of brain ischemia/reperfusion. DESIGN: Randomized and controlled observation. SETTING: Staff Room of Acupuncture and Moxibustion, Department of Acupuncture and Bone Injury, Hubei College of traditional chinese medicine; Tongji Medical College, Huazhong university of Science and Technology. MATERIALS: Seventy-two healthy SD rats, of clean degree and either gender, weighing (200±20) g, were provided by the Experimental Animal Center of Hubei College of traditional chinese medicine. STAT3 monoclonal antibody was purchased from Santa Cruz Company, USA, and G-6805 electro-acupuncture instrument was purchased from Shanghai Medical Electronic Instruments Factory. METHODS: This experiment was carried out in the comprehensive laboratory of Department of Acupuncture and Bone Injury, Hubei College of traditional chinese medicine between September 2005 and February 2006. Seventy-two rats were randomly divided into 4 groups: ① control group(n =6): Untouched. ② Sham-operation group (n =18): Artery was isolated, but without inserting thread bolt.③ Model group (n =24): Rat models of local brain ischemia/reperfusion were established with modified suture occlusion. ④Electro-acupuncture group (n =24): Dazhui and bilateral Neiguan points were selected for electro-acupuncture treatment. No. 28 acupuncture needle of 3.33 cm was used in the treatment. A G-6085 electro-acupuncture instrument with continuous wave, frequency of 120 times/min, intensity of 1 mA

关键词： STAT Electro-acupuncture for STAT3 expression and nuclear translocation in hippocampal tissues of rats following cerebral ischemia/reperfusion

Molecular Analysis of Leonurus Species in china Based on nuclear ITS and Chloroplast matK Sequences

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Molecular Analysis of Leonurus Species in China Based on Nuc...

中华中医药学会第十届中药鉴定学术会议暨WHO中药材鉴定方法和技术研讨会

作者： Zhi-Ye Yang,~(*,+) Sheng-Li Pan,~+ Ke-Ke Huo,~■ Bing-Yi Wu~■ and Zhi Chao~§* guangdong Institute for Drug Control,guangzhou 510180,china + School of Pharmacy,Fudan university,Shanghai 200032,china ■ School of Life Sciences,Fudan university,Shanghai 200433,china ■ Nanfang Hospital,Southern Medical university,guangzhou 510515,china§School of traditional chinese medicine,Southern Medical university,guangzhou 510515, china

The genus Leonurus has long been recognized as a natural group,but its interspecific relationship has not yet been studied in the light of sequence *** ITS regions and matK sequences of all subgenera of Leonurus in china were amplified,sequenced and *** generated by maximum parsimony and neighbor-joining methods and division of the genus into two major *** phylogenetic results indicated that *** has the very close phylogenetic relationship with *** and supported the notion that *** acts as the bridge between *** and *** to analysis of the information given by ITS and matK sequences,we as well suggest that ITS sequences would be more suitable to serve as markers for authentication of Herba Leonuri than matK does.

关键词： Leonurus Herba Leonuri ITS matK phylogenic analysis crude drug authentication

Effects of the combined intervention of transplantation of bone marrow messchymal stem cell transfected with VEGF gene and Naomai Yi-hao capsule on the angiogenesis in cerebral ischemic rats

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Effects of the combined intervention of transplantation of b...

国家中医药管理局脑病重点研究室建设研讨会暨中风病科研成果推广交流会

作者： Guo Jian-wen~1,Chen Chao~2,Huang Yan~1,Li Ben~1 (1 guangdong Provincial Hospital of chinese medicine,guangzhou,china 2 Pharmacy Department of the First Clinic Affiliated Hospital to Guangxi College of traditional chinese medicine.)

Objective:To assess the combined effects of Naomai Yihao(NMYH) Capsule and bone marrow messchymal stem cell(BMSC) transfected with VEGF gene transplantation on angiogenesis in cerebral rats. Method:the BMSCs were isolated and cultured from bone marrow by the holo-bone marrow adherence *** they were transfected with eukaryotic expression plasmid pEGFP-VEGF by positive ionic liposome transfection.A rat model of middle cerebral artery occlusion(MCAO) was made,and the rats were allocated to six groups,i.e. the untreated group,the BMSCs group,the VEGF gene-modified BMSCs transplantation group(BMSCs/VEGF),the NMYH group,and the NMYH and BMSCs/VEGF combined group and the sham operation *** rating scale(BRS) to rats and expression of CD34 and VEGF in the brain tissue were measured with the immunohistochemical method respectively after the 7,14 and 21 day of *** angiogenesis was observed and evaluated with laser scanning confocal microscopy(LSCM). Results:The BRS outcome of the NMYH group,the BMSCs transplantation group and the NMYH + BMSCs/VEGF combined group were lower than the untreated model group(P34 and VEGF in cells of the NMYH group,transplantation group and combined treatment group increased higher than those in the untreated group(P3). Conclusion:BMSCs transfected with VEGF gene can improve the angiogenesis in the cerebral ischemic *** capsule can promote the angiogenesis in MCAO rats treated by BMSCs transplantation and improve the BRS on neurological *** mechanism of angiogenesis may be related to the up-regulation of expression of VEG

关键词： Bone Mesenchymal Stem Cells Naomaiyihao Capsule Vascular Endothelial Growth Factor Eukaryotic Expression Plasmid Transplantation Cerebral Ischemia Angiogenesis

Salvianic Acid A Inhibits Lipopolysaccharide-induced Apoptosis through Regulating Glutathione Peroxidase Activity and Malondialdehyde Level in Vascular Endothelial Cells

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Salvianic Acid A Inhibits Lipopolysaccharide-induced Apoptos...

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Zhao Qi-Tao~1,Guo Qing-Mei~2 1.laboratory of Cytobiology,School of basic medicine,Shandong university of traditional chinese medicine,Jinan,250355,china 2.Department of Pharmacognosy,School of Pharmaceutical Sciences,Shandong university of traditional chinese medicine,Jinan 250355, china

AIM:To find out the role of salvianic acid A(SAA) in the protection of vascular endothehal cells(VEC) and its possible mechanism in ***:Added a series of this ingredient to human umbilical vein endothelial cells(HUVEC) treated with 0.5μM lipopolysaccharide(LPS) for 24 *** morphological changes of cells were observed under inverted phase contrast microscope,me cell viability was quantified using MTT assay. nuclear fragmentation of cells was observed under laser scanning confocal microcopy after being stained with *** cycle distribution was detected by the flow-cytometric after being stained with propidium iodide(PI).The activities of glutathione peroxidase(GPH-PX) as well as maleic dialdehyde(MDA) level in cells were measured by spectrophotometric methods as described in the assay ***:Apoptotic morphological changes and the decrease of cell viability of these cells were obviously inhibited by SAA in a dose-dependent ***,the abnormal cell cycle distribution,the decrease of GSH-Px activity and the increase of MDA level induced by LPS were markedly ***:SAA produces protective action on VEC induced by LPS via an antioxidative mechanism.

关键词： Salvianic acid A Vascular endothelial cells Glutathione peroxidase Malondialdehyde Lipopolysaccharide

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育龄女性重复人工流产原因分析

中国妇幼保健 2013年第1期28卷 110-112页

作者：张淑婷李雅文陈粮刁伟坚辛惠锦张娣广州中医药大学第一附属医院生殖医学科广东广州510405 广东省广州市人口和计划生育技术服务指导所妇科

目的:探讨育龄女性重复人工流产(人流)原因及相关因素。方法:收集2008年1月1日～2010年8月31日在广州市人口和计划生育技术服务指导所行人流231例,按人流次数分为单次人流组和重复人流组两组,分析患者年龄、婚姻状态、孕产史、流产史、... 详细信息

目的:探讨育龄女性重复人工流产(人流)原因及相关因素。方法:收集2008年1月1日～2010年8月31日在广州市人口和计划生育技术服务指导所行人流231例,按人流次数分为单次人流组和重复人流组两组,分析患者年龄、婚姻状态、孕产史、流产史、分娩方式和避孕方法等在两组病例中所占的比例及其与重复人流的相关性。结果:231例人流病例中,有重复人流112例,占48.48%,年龄、婚姻状态、分娩方式和所采用避孕措施均与重复人流不相关,已生育女性和无避孕中未坚持使用避孕套者与重复人流相关。结论:加强育龄女性中已生育人群的避孕宣传教育及提高选择避孕套作为避孕方式人群坚持使用避孕套的主动意识,对降低育龄女性重复人流率有重要意义。

关键词：育龄女性重复人工流产避孕

Ischemic preconditioning enhances hepatocyte proliferation in the early phase after ischemia under hemi-hepatectomy in rats

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Hepatobiliary & Pancreatic Diseases International 2012年第5期11卷 521-526页

作者： Li-Ming Jin, Shui-Fang Jin, Yuan-Xing Liu, Lin Zhou, Hai-Yang Xie, Sheng Yan, Xiao Xu , Shu-Sen Zheng Department of General Surgery and Intensive Care Unit, First Affiliated Hospital, Zhejiang chinese Medical university, Hangzhou 310006, china Key laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Key laboratory of Organ Transplantation, Zhejiang province, and Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang university School of medicine, Hangzhou 310003, china Department of General Surgery First Affiliated Hospital Zhejiang Chinese Medical University Hangzhou 310006 Hangzhou China Intensive Care Unit First Affiliated Hospital Zhejiang Chinese Medical University Hangzhou 310006 Hangzhou China Key laboratory of Combined Multi-organ Transplantation Ministry of Public Health Key Laboratory of Organ Transplantation Zhejiang Province and Division of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 Hangzhou China

BACKGROUND: Ischemia/reperfusion (I/R) injury is an important barrier to liver surgery and transplantation because it impairs remnant liver/reduced-size-graft regeneration. Ischemic preconditioning (IPC), as an effective measure to overcome I/R injury, has been shown to enhance the regenerative capacity of hepatocytes. However, investigations have always focused on regeneration in the late phase after reperfusion. This study aimed to investigate whether IPC enhances hepatocyte proliferation in the early phase after reperfusion and possible underlying mechanisms. METHODS: A total of 90 rats were divided into three groups: hemi-hepatectomy alone (PHx group), 60 minutes of ischemia plus hemi-hepatectomy (I/R group), and a cycle of 10 minutes of alternating I/R prior to 60 minutes of ischemia plus hemi-hepatectomy (IPC group). Each group was divided into five subgroups sacrificed after 0.5, 2, 6, 12 or 24 hours (n=6/ subgroup). Subsequently, serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) were measured; caspase-3 and proliferating cell nuclear antigen (PCNA) proteins were also determined by Western blotting. Furthermore, PCNA was detected by immunohistochemistry to identify the expression ***: Serum ALT and AST levels after 2-24 hours of reperfusion in the PHx and IPC groups were remarkably decreased compared to the I/R group, and the serum TNF-α was relatively lower. A significant increase of serum IL-6 levels was found in the PHx and IPC groups compared with the I/R group at each time point. Furthermore, PCNA expression was remarkably increased in the IPC group after 6-12 hours of reperfusion, and in the earlier 0.5 and 6 hours time points after reperfusion have shown the massive PCNA-positive hepatocytes. At the same time, the expression of liver p-JNK was higher in the IPC group in the early phase after reperfusion than that of the I/R group and its expre

关键词： reperfusion injury ischemic preconditioning hepatocytes proliferation hemi-hepatectomy

摹乡野映像品淳朴生活——谈王沂东的写实油画

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美术 2024年第5期 146-148页

作者：潘广东临沂大学美术学院

作为中国写实主义画家的代表,王沂东的艺术在吸收传统中国画和民族艺术的同时融入西方古典油画技法,形成了独特的绘画表现风格,在淳朴民风、自然山水等题材中展现出浓厚的审美意蕴,在色彩运用、线条处理及构图布局上追求大胆创新,为观... 详细信息

作为中国写实主义画家的代表,王沂东的艺术在吸收传统中国画和民族艺术的同时融入西方古典油画技法,形成了独特的绘画表现风格,在淳朴民风、自然山水等题材中展现出浓厚的审美意蕴,在色彩运用、线条处理及构图布局上追求大胆创新,为观众带来了视觉与情感的双重享受。

关键词：王沂东写实油画油画语言