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Immunohistochemical Studies of the Expression of Matrix Metalloproteinase-2 and Metalloproteinase-9 in Human Prostate Cancer

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Journal of huazhong university of science and technology(medical sciences) 2003年第4期23卷 373-374,379页

作者：曾汉青肖亚军鲁功成陈勇 department of Urology

To study the expression of matrix metalloproteinase 2 and 9 in human prostate cancer, matrix metalloproteinase 2 and 9 were immunohistochemically detected in tissues of prostate cancer and benign prostatic hyperplasia (BPH). Our results showed that matrix metalloproteinase 2 and 9 levels in prostate cancer were much higher than those in tissues of BPH, with the cancer invasion being positively correlated with the expression of the metalloproteinases. It is concluded that matrix metalloproteinase 2 and 9 are better molecular markers, which are of help in the diagnosis and prediction of prognosis of prostate cancer.

关键词： prostate cancer matrix metalloproteinase 2 matrix metalloproteinase 9 immunohistochemistry

Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion

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Acta Pharmacologica Sinica 2004年第4期25卷 98-104页

作者： San-hua LENG, Fu-er LU2, Li-jun XU Institute of Integrated Traditional Chinese & Western Medicine, tongji hospital, tongjimedical college, huazhong university of science & technology, wuhan 430030, china Institute of Integrated Traditional Chinese & Western Medicine Tongji Hospital Tongji Medical College Huazhong University of Science & Technology China

AIM: To explore the anti-diabetic effects of berberine and its influence on insulin secretion. METHODS: Impaired glucose tolerance rats induced by iv injection of streptozotocin 30 mg/kgwere treated with berberine 187.5 and 562.5 mg/kg while fed with high fat laboratory chow. After rats were treated for 4 weeks, oral glucose tolerance was determined, and for 8 weeks, the fasting blood glucose, insulin, lipid series were determined. In insulin secretion experiments, berberine 93.75, 187.5, and 562.5 mg/kg was administered orally to BALB/c mice at a bolus. The murine serum was collected 2 h after the berberine administration for insulin determination. Insulin released from HIT-T15 cells and pancreatic islets incubated with berberine 1-100 μmol/L for 12 h was determined. RESULTS: The levels of fasting blood glucose (7.4±1.5 or 7.3±1.3 vs 9.3±1.3 mmol/L), triglycerides (0.61±0.22 or 0.63± 0.17 vs 1.8±0.7 mmol/L), total cholesterol (1.8±0.3 or 1.9±0.3 vs 2.2±0.2 mmol/L), free fatty acid (456±93 or 460±72 vs 550±113 μmol/L) and apolipoprotein B (0.37±0.02 or 0.42±0.05 vs 0.46±0.04 g/L) were reduced greatly in berberine-treated groups at doses of 187.5 and 562.5 mg·kg-1·d-1, respectively as compared with those in control group (P3 or 1.4±0.3vs 1.1±0.1 g/L), apolipoprotein AI (0.80±0.08 or 0.87±0.08 vs 0.71±0.06 g/L) were significantly increased (P3±2.8 pIU/L) was undoubtedly promoted and blood glucose (4.52±0.31 or 4.45±0.29 or 4.30±0.19 vs 4.87±0.21 mmol/L) was reduced after berberine administration at doses of 93.75, 187.5, and 562.5 mg/kg, respectively in the BALB/c mice. CONCLUSION: Berberine possesses anti-diabetic effec

关键词： berberine diabetes mellitus glucose tolerance test insulin HIT-T15 cells pancreatic islets lipids

FRACTAL GEOMETRY STUDY OF CORRELATION BETWEEN IMPACT TOUGHNESS OF STEEL AND PARAMETERS OF FREE-CUTTING PHASE

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Acta Metallurgica Sinica(English Letters) 1992年第4期5卷 250-255页

作者： JIANG Laizhu CUI Kun huazhong university of science and technology,wuhan,china huazhong Univ of science and Technology Wuhan China

Studies were made of the calculation of fractal dimension of transverse impact fracture sur- face,and of the correlation between impact toughness of steel and parameters of free-cutting phase by means of the developed fractal geometry model of crack *** is believed that the area fraction,f,of free-cutting phase is negligibly influential to the longitudinal im- pact toughness,as f1 .While the aspect ratio,saying ratio of length to width,of free-cut. ting phase is inversely influential to the transverse impact *** may .be the reason why the transverse impact toughness of free-cutting steel containing more rare earth contrast to sulphur is even higher than the low sulphur containing steel.

关键词： fractal geometry impact toughness free-cutting phase

Prediction of Trend between Water Environment Pollution of D Lake and Death Rate of Malignancy in Population

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Journal of huazhong university of science and technology(medical sciences) 2003年第4期23卷 420-422页

作者：李龙吴春松 Institute of Environmental Medicine department of Mathematics

Grey system analysis method was used to study the correlation between water pollution in D Lake area and death rate of malignancy with death rate of malignancy as effect sequence and a variety of water pollution index as factor sequence. On the basis of grey correlation analysis, grey system predication model was established for death rate of malignancy in population in D Lake area including GM (1, N) model for death rate of malignancy [ MR (t+1) =(9.9987E 1+5.0001E 2 +10.8994E 3+1.1114E 4+165.1029) ·e -0.0070t -9.9987E 1-5.0001E 2-10.8994E 3-1.1114E 4 ] and GM (1, 1) model for related factors [ E 1(t+1) =52.1214-46.9468e -0.0058t , E 2(t+1) =4.6114-4.5664e 0.0015t, E 3(t+1) =1.1389-1.1212e 0.0065t , E 4(t+1) = 554.5867-549.8006e 0.0016t ], and the trend of death rate of malignancy from 2000 to 2010 was predicted.

关键词： death rate of malignancy grey system water environment pollution

Inhibition of Inositol-1-phosphate Synthetase in Mycobacterium Tuberculosis by Chitosan-antisense Nanoparticles

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Journal of wuhan university of technology(Materials science) 2009年第1期24卷 87-90页

作者：李媛媛徐顺清 MOE Key Laboratory School of Public Health Tongji Medical College Huazhong University of Science and Technology

Oligodeoxynucleotides （ODNs） were combined with the biodegradable polymer chitosan to form chitosan-ODN nanoparticles by complex coacervation, in order to improve the sta-bility and intracellular penetration. The diameter of the nanoparticles was light strength size and ranged between 60 and 219 nm with a mean value of 132 nm, while zeta potential was between ＋12 and ＋20 mV at pH 5.5. The chitosan-ODN nanoparticles could partially protect the encapsulated ODN from nuclease degradation. Moreover, chitosan-ODN nanoparticles were much more effective in inhibiting the proliferation of *** than free ODN.

关键词： chitosan antisense oligodeoxynucleotides nanoparticle tuberculosis

Preparation and Preservation of Hypoxia UW Solution

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Journal of huazhong university of science and technology(medical sciences) 2007年第5期27卷 554-556页

作者：万赤丹王春友刘涛程锐杨智勇 department of general surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology

In order to explore the method to prepare hypoxia UW solution and the stability and preservation of hypoxia UW solution, UW solution was purged by argon or air for 15 min or 60 at a flow rate of 0.8 or 2 L/min, and the oxygen partial pressure of UW solution was detected. The hypoxia UW solution was exposed to the air or sealed up to preserve by using different methods, and the changes of oxygen partial pressure was tested. The results showed that oxygen partial pressure of 50 mL UW solution, purged by argon for 15 min at a flow rate of 2 L/min, was declined from 242±6 mmHg to 83±10 mmHg. After exposure to the air, oxygen partial pressure of hypoxia UW solution was gradually increased to 160±7 mmHg at 48 h. After sealed up by the centrifuge tube and plastic bad filled with argon, oxygen partial pressure of hypoxia UW solution was stable, about 88±13 mmHg at 72 h. It was concluded that oxygen of UW solution could be purged by argon efficiently. Sealed up by the centrifuge tube and plastic bag filled with argon, oxygen partial pressure of UW solution could be stabilized.

关键词： organ preservation solution oxygen partial pressure hypoxia

Low and Maternal-specific Expression of p57^(KIP2) in Hydatidiform Mole and Its Clinical Implication

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Journal of huazhong university of science and technology(medical sciences) 2002年第2期22卷 121-122,157页

作者：熊雅丽曹阳李红发 department of Obstetrics and Gynecology Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Department of Ophthalmology Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Department of Obstetrics and Gynecology Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022

Summary: In situ hybridization was applied to locate and detect the expression of p57 KIP2 in hydatidiform mole (5 cases of partial hydatidiform mole and 18 cases of complete hydatidiform mole) and normal villi (23 cases). The positive signals of p57 KIP2 expression were analyzed by HPIAS-1000 Image-Analysis System. p57 KIP2 was highly expressed in normal villi but showed distinct low expression in hydatidiform mole (P<0.01). Furthermore, the locus of low expression of p57 KIP2 accorded with the place where lesion of trophoblast occurred. Detection of p57 KIP2 made it possible to study the genetics of hydatidiform mole at the transcriptional level. Low expression of p57 KIP2 could be a molecular marker in hydatidiform mole and a target for therapy.

关键词： p57 KIP2 hydatidiform mole in situ hybridization

Down-regulation of the gax gene in smooth muscle cells of the splenic vein of portal hypertension patients

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 242-245页

作者： Jin-Hua Zeng, Zhen Yang, Jun Xu, Ming-Lian Qiu and Ke-Can Lin department of surgery, First Affiliated hospital of Fu-jian medical university, Fuzhou 350004 , china department of surgery, tongji hospital, tongji medical college, huazhong university of science and technology, wuhan 430030, china department of surgery First Affiliated Hospital of Fujian Medical University Fuzhou 350004 China. snake4311@***

BACKGROUND: The expression of gax, an anti-prolifera-tive homeobox gene, is rapidly down-regulated in vascular smooth muscle cells (VSMCs) following arterial injury. Whether the down-regulation of gax is involved in modulating the proliferation of smooth muscle cells of the splenic vein in patients with portal hypertension has not yet been elucidated. The aim of this study was to investigate the expression of the mRNA of the gax gene in smooth muscle cells of the splenic vein in patients with portal hypertension. METHODS: Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of gax mRNA and immunohistochemistry staining was performed to demonstrate the expression of PCNA protein in the splenic veins of 28 patients with portal hypertension and those of 12 normal controls. This study was approved by the Institutional Ethics Committee and informed consent was obtained from all participants. RESULTS: RT-PCR showed that the expression of gax mRNA was PCNA-positive and negative in the splenic vein of patients with portal hypertension (1.08 ± 0.04 and 1.39 ± 0.27, respectively). There was a significant difference in the 28 patients compared with the 12 normal controls ( P < 0.01). The relative expression of PCNA protein in the vascular tissues was significantly higher in the experimental group than in the control group. CONCLUSIONS: Down-regulation of gax mRNA and the overexpression of PCNA protein were seen in smooth muscle cells of the splenic vein in patients with portal hypertension, regulating the proliferation, migration and phenoty-pic change and resulting in remodelling of the splenic vein, which may play an important role in the pathogenesis and maintenance of portal hypertension.

关键词： hypertension portal vein vascular smooth muscle cell proliferation gene expression

Effect of Transforming Growth Factor-β_2 on Phagocytosis in Cultured Bovine Trabecular Meshwork Cells

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Journal of huazhong university of science and technology(medical sciences) 2001年第4期21卷 318-320页

作者：曹阳魏厚仁笪邦红黄毅 department of Ophthalmology Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022

The effect of transforming growth factor β 2 (TGF β 2) on phagocytosis in bovine trabecular meshwork cells in vitro was investigated. After the cultured bovine trabecular meshwork cells were treated with 0 ng/ml, 0.32 ng/ml, 1 ng/ml, 3.2 ng/ml TGF β 2 for 24 h, latex beads were added into the incubation medium, and the numbers of the latex beads in 20 adjacent cells were counted under a microscope 24 h later, after treatment with Wright's stain. Our results showed that the average numbers of the latex beads in the trabecular meshwork cells treated with TGF β 2 of different concentrations were 53.1±1.7 beads/cell, 56.4±2.9 beads/cell and 77.9±6.5 beads/cell respectinvely, in comparison with 45.5±3.3 beads/cell of the control group. TGF β 2 significantly increased the number of the latex beads phagocytosed by cultured bovine trabecular meshwork cells in a dose dependent manner. TGF β 2 could promote the phagocytosis of bovine trabecular meshwork cells in vitro . It may be involved in the cellularity decrease of the trabecular meshwork in the patients of primary open angle glaucoma through promoting the phagocytosis of trabecular meshwork cells.

关键词： transforming growth factor trabecular meshwork cultured cells phagocytosis