检索结果-南通市图书馆

The Controlled-releasing Drug Implant based on the Three Dimensional Printing technology: Fabrication and Properties of Drug Releasing in vivo

在线全文

学校读者我要写书评

暂无评论

Journal of wuhan university of technology(Materials science) 2009年第6期24卷 977-981页

作者：伍卫刚郑启新 department of orthopedics Union HospitalTongji medical collegeHuazhong University of Science and Technology

Three dimensional （3D） printing technology was utilized to fabricate a new type of drug implant with complicated architectures, employing levofloxacin （LVFX） and rifampicine （RFP） as model drugs. The prepared drug implant prototype consists of a doublelayer structure, of which the upper region is a reservoir system containing RFP and the lower region is a matrix one containing LVFX. The release test in vivo revealed that LVFX was released in the early stage; no RFP was detected until 8th day; both of them continuously released more than 6 weeks. Therefore, 3D printing technology provides a precise and feasible method to fabricate a controlled-releasing drug implant with complicated architectures and this drug implant may present a new strategy for the prophylaxis and treatment of bone diseases such as combined bone infections and bone tuberculosis in the near future.

关键词： rapid prototyping three dimension printing technology drug implants bone in- fection bone tuberculosis

PEGylated PLGA Nanoparticles as Tumor Ecrosis Factor-α Receptor Blocking Peptide Carriers:Preparation,Characterization and Release in vitro

在线全文

学校读者我要写书评

暂无评论

Journal of wuhan university of technology(Materials science) 2007年第1期22卷 112-116页

作者：刘卫杨祥良 college of Life science and technology Huazhong University of Science and Technology

To assess the merits of PEGylated poly （lactic-co-glycolic acid）（PEG-PLGA） nanoparticles as drug carriers for tumor necrosis factor-α receptor blocking peptide （TNFR-BP）, PEG-PLGA copolymer, which could be used to prepare the stealth nanoparticles, was synthesized with methoxypolyethyleneglycol, DL-lactide and glycolide. The structure of PEG-PLGA was confirmed with ^1H-NMR and FT-IR spectroscopy, and the molecular weight （MW） was determined by gel permeation chromatography. Fluorescent FITC-TNFR- BP was chosen as model protein and encapsulated within PEG-PLGA nanoparticles using the double emulsion method. Atomic force microscopy and photon correlation spectroscopy were employed to characterize the stealth nanoparticles fabricated for morphology, size with polydispersity index and zeta potential. Encapsulation efficiency （EE） and the release of FITC-TNFR-BP in nanopartieles in vitro were measured by the fluorescence measurement. The stealth nanoparticles were found to have the mean diameter less than 270 nm and zeta potential less than -20 mV. In all nanoparticle formulations, more than 45% of EE were obtained. FITC-TNFR-BP release from the PEG-PLGA nanoparticles exhibited a biphasic pattern, initial burst release and consequently sustained release. The experimental results show that PEG-PLGA nanoparticles possess the potential to develop as drug carriers for controlled release applications of TNFR-BP.

关键词： tumor necrosis factor-α receptor blocking peptide PEG-PLGA stealth nanoparticles ring-opening polymerization controlled and sustained drug release

Subacute Thyroiditis as Seen Initially in ENT department—— A Reportof30 Cases

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2002年第1期22卷 82-83页

作者： WANG Zhibin (王志斌) department of Otolaryngology Tongji Hospital Tongji Medical College Huazhong University of Science and Technolog y Wuhan 430030

To assess the pharyngeal presentations and the diagnostic value of thyroid SPECT and thyroid fine needle aspiratory biopsy (FNAB) in subacute thyroiditis (SAT) as seen initially in ENT department, 30 patients, during the course of SAT, were examined for pharyngeal symptoms and tested for serum T 3, T 4 level. The thyroid SPECT imaging or thyroid FNAB were performed. Our results showed that, of the 30 patients, 21 had sore throat of various degrees, and 9 had abnormal sensation of throat. Six were diagnosed as having SAT by only SPECT, in the remaining 24, the final diagnoses was established by SPECT combined with FNAB. Two of them were finally diagnosed as having SAT by trial treatment with oral prednisone. It is concluded that sore throat and abnormal sensation of pharynx are the important presentations of SAT, and thyroid SPECT imaging and thyroid FNAB are valuable in diagnosing SAT.

关键词： subacute thyroiditis sore throat abnormal sensation of pharynx

Expression and Clinical Implication of HIF-1α and VEGF-C in Non-small Cell Lung Cancer

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2008年第6期28卷 674-676页

作者：左顺庆季艳梅王建军郭家龙 department of Thoracic surgery Union HospitalTongji Medical CollegeHuazhong University of Science and Technology department of Cardiothoracic surgery Taihe Hospital Affiliated to Yunyang Medical College department of Intensive Care Unit Taihe Hospital Affiliated to Yunyang Medical College

To study the expression and implication of HIF-1α and VEGF-C in non-small cell lung cancer （NSCLC） and its relationship with clinical pathological features of NSCLC, immunohisto-chemical SP was used to detect the expression of HIF-1α and VEGF-C proteins in 48 NSCLC tissues and the same para-cancerous tissues. The positive rates of HIF-1α and VEGF-C were 70.8% （34/48） and 68.8% （33/48） respectively. The expression of HIF-1α protein was detected in a significantly greater proportion in NSCLC carcinoma tissues than that in para-cancerous tissues （12.5% and 16.7%, P〈0.05）. The positive rates of HIF-1α and VEGF-C were correlated with lymph node metastasis and TNM stage. No relationship was found between the two factors and age, sex, pathological subtypes and histological grades. The positive rates between HIF-1α and VEGF-C were correlated （P〈0.05）. HIF-1α and VEGF-C were over-expressed in NSCLC. They may be involved in the carcinogenesis of NSCLC, and play an important role in invasion and metastasis of NSCLC. HIF-1α and VEGF-C work synergically in the process of NSCLC.

关键词： lung neoplasm hypoxia-inducible factor-1α vascular endothelial growth facor-C

A Modified Method for Preparation of Adriamycin Carried by Magnetic Albumin Microspheres

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2001年第3期21卷 233-235页

作者：马建华陈道达田源 department of general surgery Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022

The targeting of antineoplastic agents to restricted anatomic sites and specific target cells have been challenged clinicians all the time in cancer chemotherapy, which resulted in recent efforts to focus the effects of existing antitumor agents and treatments on tumor cells and spare their effects on normal cells. The drug-carrier complex, adriamycin carried by magnetic albumin microspheres (ADM-MAM) was prepared by using our discovered new and modified method. The physical feature of the prepared drug-carrier microspheres was much better than by the traditional method in comparison. The successful preparation of the drug-carrier complex, ADM-MAM, is one of the key steps for our later further researches in the targeted chemotherapy.

关键词： adriamycin targeted chemotherapy magnetic albumin microspheres

Inhibitory Effect of HCPT on Expression of HIF-1α and Downstream Genes in Hypoxic Human Cervical SiHa Cancer Cells

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2007年第5期27卷 586-589页

作者：施薇于世英 Cancer Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

The hypoxic model to simulate hypoxic microenvironment in solid tumors was established and the effect of hydrocamptothecin （HCPT） on the hypoxia-induced over-expression of HIF-1α and VEGF genes was explored. Human cervical cancer SiHa cells were cultured in vitro under hypoxic conditions （37℃, 5% CO2, 1%O2） and treated with different concentrations of HCPT for 24 h. The mRNA and protein expression levels of HIF-1α, VEGF and Glutl in SiHa cells were detected by semi-quantitative RT-PCR and Western blot respectively. Normoxic control groups were exposed to normoxic conditions for 24 h. Under normoxic conditions, HCPT had no obvious effects on the HIF-1α and VEGF gene expression. Hypoxia induced the up-regulation of HIF-1α protein and downstream VEGF gene, and HCPT showed a dose-dependently inhibitory effect on the hypoxia-induced over-expression of HIF-1α protein and VEGF gene expression in SiHa cells, whereas HCPT had no significant effect on the HIF-1α mRNA expression. No difference in HCPT cytotoxic- ity was observed between hypoxic groups and normoxic control groups. It was suggested that HCPT could inhibite the expression of HIF-1α protein and downstream VEGF gene in hypoxic SiHa cells in a dose-dependent manner, and the inhibitory effect was not related with HCPT cytotoxicity.

关键词： hydrocamptothecin HIF-1α VEGF cervical cancer

Effect of amyloid peptides on serum withdrawal-induced cell differentiation and cell viability

在线全文

学校读者我要写书评

暂无评论

Cell Research 2004年第6期14卷 467-472页

作者： Yi Peng WANG, Ze Fen WANG, Ying Chun ZHANG, Qing TIAN, Jian Zhi WANG department of Pathophysiology, tongji medical college, huazhong university of science and technology, wuhan, 430030, china department of Pathophyiiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Abnormal deposition of amyloid-p(Ap) peptides and formation of neuritic plaques are recognized as pathological processes in Alzheimer’s disease (AD) brain. By using amyloid precursor protein (APP) transfected cells, this study aims to investigate the effect of overproduction of Aβ on cell differentiation and cell viability. It was shown that after serum withdrawal, untransfected cell (N2a/Wt) and vector transfected cells (N2a/vector) extended long and branched cell processes, whereas no neurites was induced in wild type APP (N2a/APP695) and Swedish mutant APP (N2a/ APPswe) transfected N2a cells. After differentiation by serum withdrawal, the localization of APP/AP and neurofilament was extended to neurites, whereas those of APP-transfected cells were stillrestricted within the cell body. Levels of both APP and Aβ were significantly higher in N2a/APP695 and N2a/APPswe than in N2a/Wt, as determined by Western blot and Sandwich ELISA, respectively. To further investigate the effect of A0 on the inhibition

关键词： Alzheimer’s disease amyloid β cell differentiation.

Expression of Mesenger RNA for Transforming Growth Factor-β_1 in Bovine Trabecular Meshwork

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Eye science 1996年第1期16卷 1-4页

作者： Liya Yuan, Houren Weidepartment of Ophthalmology, union hospital, tongji medical university, wuhan 430022,china department of Ophthalmology Union Hospital Tongji Medical University Wuhan China.

Purpose: To investigate the relationship between transforming growth factor-β1(TGF-β1) and primary open-angle glaucoma, we have determined whether trabec-ular tissues have the expression of messenger RNA for TGF-β***: Total RNA of 24 newborn bovine trabecular tissue were extracted byGuanidine isothiocyanate method. The TGF-β33 plasmid was brought into E. col-ibacillius HB101 and amplificated. After Bam HI endolase degradation and labelwith a-32p-dATP the RNA was hybridized with the cDNA (complementary DNA)probe and examined by ***: The presence of mRNA for TGF-β1 in bovine trabecular meshwork ***: The TGF-β1 present in normal aqueous humor must be at least partlyderived from the trabecular meshwork. It offered a basis for understanding therelationship between abnormal synthesis, activation and clearance of TGF-β1 andthe pathogenesis of primary open-angle glaucoma (POAG) in molecular *** science 1996; 12:1-4.

关键词： trabecular meshwork transforming growth factor-β1 open-angle glaucoma

Effect and Mechanism of Superantigen Staphylococcal Enterotoxin Therapy for Mouse Gastric Tumor

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2003年第2期23卷 161-162页

作者：李毅清舒晓钢 department of general surgery Xiehe HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430022

The anti-tumor effect and mechanism of the staphylococcal enterotoxin A (SEA) were studied. The mouse gastric tumor model was produced by subcutaneously inoculating gastric tumor ceils (MGC80-3). The experimental group was treated with SEA, and the control group was treated with normal saline. The percentage of tumor generation and tumor mass was measured. The results showed that the percentage of the tumor generation in the SEA-treated mice was lower than in the control group, but there was no significant difference (P>0. 05). However, the tumor mass in the experimental group was significantly lighter than in the control group, with the difference being very significant (P<0. 001). There were more CD4+ T cells and CD8+ T cells in the tumor of the mice treated with SEA than those of the control group. SEA has an obvious anti-tumor effect on mice gastric tumor. The mechanism might be that SEA induces the effect of superantigen-dependent cell mediated cytotoxicity to the tumor cells.

关键词： superantigen staphylococcal enterotoxin A gastric tumor