Expression of Mesenger RNA for Transforming Growth Factor-β_1 in Bovine Trabecular Meshwork

作     者：Liya Yuan, Houren WeiDepartment of Ophthalmology, Union Hospital, Tongji Medical University, Wuhan 430022,China 

作者机构：Department of Ophthalmology Union Hospital Tongji Medical University Wuhan China. 

出 版 物：《Eye Science》 (眼科学报（英文版）)

年 卷 期：1996年第16卷第1期

页      面：1-4页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：National Natural Science Function  No. 38970755 

主　　题：trabecular meshwork transforming growth factor-β₁ open-angle glaucoma 

摘      要：Purpose: To investigate the relationship between transforming growth factor-β1(TGF-β1) and primary open-angle glaucoma, we have determined whether trabec-ular tissues have the expression of messenger RNA for TGF-β***: Total RNA of 24 newborn bovine trabecular tissue were extracted byGuanidine isothiocyanate method. The TGF-β33 plasmid was brought into E. col-ibacillius HB101 and amplificated. After Bam HI endolase degradation and labelwith a-32p-dATP the RNA was hybridized with the cDNA (complementary DNA)probe and examined by ***: The presence of mRNA for TGF-β1 in bovine trabecular meshwork ***: The TGF-β1 present in normal aqueous humor must be at least partlyderived from the trabecular meshwork. It offered a basis for understanding therelationship between abnormal synthesis, activation and clearance of TGF-β1 andthe pathogenesis of primary open-angle glaucoma (POAG) in molecular *** Science 1996; 12:1-4.