检索结果-南通市图书馆

NONLINEAR STABILITY OF NATURAL RUNGE-KUTTA METHODS FOR NEUTRAL DELAY DIFFERENTIAL EQUATIONS

在线全文

学校读者我要写书评

暂无评论

Journal of Computational Mathematics 2002年第6期20卷 583-590页

作者： Cheng-jian Zhang(department of Mathematics, huazhong university of science and technology, wuhan 430074, Hubei, P. R. china) department of Mathematics Huazhong University of Science and Technology Wuhan 430074

Presents the stability analysis of theoretical solutions for a class of nonlinear neutral delay-differential equations (NDDE). Discussion on the numerical analogous results of the natural Runge-Kutta (NRK) methods for the same class of nonlinear NDDE; Review of the related concepts and results on RK methods; Information on the asymptotic stability and global stability of the induced NRK method.

关键词： nonlinear stability neutral delay differential equations natural Runge-Kutta methods

Effects of β-aescin on apoptosis induced by transient focal cerebral ischemia in rats

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第10期 27-35页

作者： Xia-min HU, Yan ZHANG, Fan-dian ZENGInstitute of Clinical Pharmacology department of Anatomy, tongji medical college, huazhong university of science and technology, wuhan 430030, china

ABM: To investigate the effects ofβ-aescin on apoptosis induced by transient focal brain ischemia in rats. METHODS: Rats were pretreated with β-aescin for 7 d and then subjected to brain ischemia/reperfusion (I/R) injury induced by a middle cerebral artery occlusion. After 2 h ischemia and 24 h reperfusion, Hematoxylin-Eosin (HE) staining, in situ end-labeling of nuclear DNA fragmentation (TUNEL) were employed to determine the level of apoptosis. The expressions of caspase-3 and Bcl-2 in the cortex were determined by immunohistochemistry and Western blot. The release of cytochrome c was analyzed by Western blot. RESULTS: The increased numbers of HE- and TUNEL-positive staining cells were significantly observed at 24 h after reperfusion. The immunoreactivity was inhibited by β-aescin (30, 60 mg/kg) (P3 was activated, whereas Bcl-2 expression was inhibited. β-Aescin (30, 60 mg/kg) markedly in

关键词： apoptosis beta-aescin brain ischemia cytochromes c caspases bcl-2 genes

Curcumin,a potent anti-tumor reagent,is a novel histone deacetylase inhibitor regulating B-NHL cell line Raji proliferation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第5期26卷 603-609页

作者： Hong-li LIU~2,Yan CHEN~(2,3),Guo-hui CUI~2,Jian-feng ZHOU~4~2department of Hematology,union hospital,tongji medical college,huazhong university of science and technology,wuhan 430022,china.~4department of Hematology.tongji hospital,tongji medical college,huazhong university of science and technology,wuhan430030,china departmentofHematology UnionHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430022

Aim:To investigate curcumin(diferuloylmethane)induced apoptosis and its mo-lecular mechanism of action in B-NHL cell line Raji ***:Raji cells werecultured in RPMI-1640 medium and treated with curcumin in different concentra-tions.3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium(MTT)assay wasused to detect growth inhibition and Hoechst 33258 staining was used to *** and Western blot were used to detect the ex-pressions of histone deacetylase 1,3,and 8(HDAC 1,HDAC3,and HDACS)andacetylated histone H4(Ac-histone H4)***:Curcumin inhibited theproliferation of B-NHL cell line Raji cells with a 36-h IC50value of 24.1±2.0μmol/*** 33258 staining showed that curcumin could induce Raji cell *** expression levels of HDAC1,HDAC3,and HDAC8 proteins were downregulat-ed following curcumin treatment in Raji cells,whereas Ac-histone H4 protein expres-sion was upregulated after treatment with ***:Curcumin,as anew member of the histone deacetylase inhibitors,can inhibit the expression ofclass I HDACs(HDAC1,HDAC3,and HDAC8),and can increase the expressionof Ac-histone H4 in Raji *** plays an important role in regulatingB-NHL cell line Raji cell proliferation and apoptosis.

关键词： curcumin histone deacetylase acetylated histone H4 Raji

Experimental Study on Inhibition of Neuronal Toxical Effect of Levodopa by Ginkgo Biloba Extract on Parkinson Disease in Rats

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2003年第2期23卷 151-153页

作者：曹非孙圣刚童萼塘 department of Neurology Xiehe HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430022

In order to observe neuronal toxical effect of Levodopa and investigate if using Levodopa together with Ginkgo Bilobar Extract (EGb) would be an workable method to treat Parkinson disease, rat models of Parkinson disease (PD) were made by injecting 6-OHDA stereotaxically to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). Rotational behavioral observation, TUNEL, immunocytochemistry, Nissl's body staining were performed to measure the difference between group treated by Levodopa (50 mg/kg every day for 3 days, 5 days, 7 days, L-dopa group) and group treated by Levodopa combined with EGb (100 mg/ kg every day, E-D group). The results showed that in the L-dopa group, the numbers of apoptosis of substantial nigra, rings of rotational behavior were more than those in the E-D group (P<0. 05). The numbers of Nissl's cells in L-dopa group were fewer than in E-D group (P<0. 05). The results suggested that Levodopa had neur toxic effect and EGb may decrease the toxicity of levodopa. The combined use of EGb with Levodopa may be a workable method to treat PD and may be better than using Levodopa alone.

关键词： ginkgo biloba extract apoptosis L-dihydroxy-phenylalanine

DYNAMIC SIMULATION OF ROTATING SHELLS COUPLED WITH LIQUID

在线全文

学校读者我要写书评

暂无评论

Acta Mechanica Solida Sinica 2001年第2期14卷 169-175页

作者： Wei Fayuan Li Shiqi Huang Yuying~1 Zhong Yifang (college of Mechanical science and Engineering, huazhong university of science and technology, wuhan 430074, china) (1 department of Mechanics, huazhong university of science and technology, wuhan 430074,china) ***

The dynamic behaviors of rotating shells coupled with liquid areshown. The shell under con- sideration has arbitrary boundaryconditions and a complex shape. A modified boundary element methodand finite strip technique are used to improve the computingefficiency and to guarantee the continuity conditions on theliquid-shell interaction plane. The effects of various parameterssuch as shell's thickness and liquid depth are investigated. Dynamicsimulations are applied to several typical shell-liquid systems, andthe natu- ral frequencies, mode shapes and response of vibration arecalculated numerically.

关键词： rotting shells dynamic simulation fluid-solid interaction

Effect of antisense DNMT3b gene eukaryotic expression plasmid on expression of the DNMT3b gene in human biliary tract carcinoma cells

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2006年第1期5卷 123-128页

作者： Shi Zuo, Jing-Qing Dong, Wei Guo, Min-Feng Liu, Li-Ning Xu, Jian Luo and Sheng-Quan Zou department of general surgery, Affiliated tongji hospital, tongji medical college, huazhong university of science & technology, wuhan 430030, china department of general surgery Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 China.

BACKGROUND: Hypermethylation of the promoter region is one of the major mechanisms of tumor suppressor gene inactivation. DNA methyltransferase 3b (DNMT3b), an enzyme that participates in the establishment of de novo methylation patterns, is highly expressed in many tumor cells and tissues, and it is closely associated with hypermethylation of the promoter of tumor suppressor genes. The aim of this study was to explore the effect of transfection with antisense DNMT3b gene eukaryotic expression plasmid on the expression of the DNMT3b gene in human biliary tract carcinoma cell. METHODS: The constructed antisense DNMT3b gene eukaryotic expression plasmid was transfected into the human biliary tract carcinoma cell line QBC-939 with lipofectamine transfection reagent, and positive cell clones were formed using G418 selection after transfection. The constructed recombinant plasmid was transfected into QBC-939 cells successfully and was confirmed by amplification of the exogenous neo^R gene with the polymerase chain reaction method. The expression of DNMT3b gene mRNA and protein was detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry respectively. RESULTS: Following transfection, the mRNA level of the DNMT3b gene decreased from 0.956±0.053 to 0.209±0.023, and the protein level of the DNMT3b gene also decreased from (75.38±3.22)% to (29.87±3.46)%. Very significant differences were observed both at the transcription and posttranscription levels in the expression of the DNMT3b gene between the non-tranfection group and the antisense DN- MT3b gene eukaryotic expression plasmid transfection group (P3b gene eukaryotic expression plasmid can significantly reduce the expression level of the DNMT3b gene in the human biliary tract carcinoma cell line QBC-939. This study may provide a valid method to investigate the function of the DNMT3b gene and its role in biliary tract c

关键词： DNA methyltransferase 3b antisense RNA transfection gene expression biliary tract carcinoma

Radioimmunoassay of Antiarrhythmic Peptide and Its application

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2003年第1期23卷 44-47页

作者：吴志坚张永学 departmentofNuclearMedicine XieheHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology

To establish a specific and sensitive radioimmunoassay (RIA) for antiarrhythmic peptide (AAP) and study serum AAP levels in normal Sprague-Dawley rats (NR) and spontaneous hypertension rats (SHR), AAP-bovine serum albumin (BSA) conjugate was prepared by ammonium bicarbonate method. The New Zealand rabbits were immunized by administering intradermally the conjugate. Then the rabbit anti-AAP serum was produced and iodinated AAP was made by Bolton-Hunter method. The RIA for serum AAP was set up and serum AAP levels in NR and SHR were determined. The minimal detectable range of the AAP RIA was (0.45±0.06) μg/L. The affinity constant for antiserum was 1.05×10 9 L/mol, and the rate of cross-reactivity with atrial peptide (AP) and human growth hormone (hGH) were 0.12 % and 0.20 %, respectively. The mean recovery rate of high, medium and low doses was 97.6 %, and the mean coefficients of variation for intra- and interbath-assay were (6.43±0.85) % and (9.62±1.04) %, respectively. The mean levels of AAP in NR with different age (3 months, 8-10 months and 18-20 months) were (1.75±0.13) μg/L, (1.74±0.11) μg/L and (1.79±0.15) μg/L, respectively, while those in SHR with different age (3 months, 8-10 months and 18-20 months) were (2.38±0.35) μg/L, (2.54±0.25) μg/L and (2.83±0.21) μg/L, respectively. The levels of serum AAP showed a positive correlation with blood pressure (r=0.8667, P<0.05). It was indicated that this AAP RIA had high specificity, high accuracy and good reproducibility. The levels of serum AAP had a close relation with blood pressure.

关键词： antiarrhythmic peptide radioimmunoassay blood pressure spontaneous hypertension rats

Effect of Pressure on Intracardiac Backscatter from Microbubbles

在线全文

学校读者我要写书评

暂无评论

Journal of huazhong university of science and technology(medical sciences) 2001年第3期21卷 246-248页

作者：邓又斌黎春蕾常青 department of medical Ultrasound Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

The backscatter from sonicated albumin microbubbles (Albunex) was analyzed using acoustic densitometry in an in vitro pulsatile heart model to evaluate the effects of pressure on the backscatter from Albunex, and the cardiac cyclic changes of intracardiac backscatter from sonicated albumin microbubbles in 16 healthy persons were analyzed. It was found that the Albunex microbubbles were compressed in systole and decompressed in diastole, causing corresponding changes of backscatter in cardiac cycle. Although the intensities of backscatter in diastole and systole were related to the concentration of microbubbles, the concentration of microbubbles had no effect on the difference of end-diastolic and end-systolic backscatter. The difference of the backscatter was highly correlated with end-systolic pressure (r=0.96, P=0.001). In human studies, we also observed same intracardiac cyclic changes of backscatter from sonicated albumin microbubbles. Our study indicates that it is possible to evaluate the intracardiac pressure non-invasively by analyzing the intracardiac backscatter from the microbubbles with acoustic densitometry.

关键词： ultrasound backscatter microbubbles intracardiac pressure

Cell proliferation in the endolymphatic sac in situ during the immune response of inner ear

在线全文

学校读者我要写书评

暂无评论

Journal of Otology 2006年第2期1卷 124-127页

作者： XIAO Hong-jun, HUANG Xiao-wen, WANG Ji-bao, HUANG Xiang department of Otorhinolaryngology, union hospital, tongji medical college, huazhong university of science & technology, wuhan 430030, People’s Republic china department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan 430030 People’s Republic China

Background Normally, few immunocompetent cell are present in the endolymphatic sac (ES). During an active immune response in the inner ear, large amount of inflammatory cells, including immunocompetent cells, are seen in the ES. The current study aimed at assessing cellular proliferation within the ES during induced immune response in the inner ear. Methods Fifteen healthy, female SD rats were sensitized systemically with keyhole limpet hemocyanin (KLH), followed by local inoculation in the cochlea through basal turn fenestration with the same antigen. On Days 3, 7 and 14 following inoculation, the animal was sacrificed after intraperitoneal administration of 5-bromo-2’-deoxyuridine (BrdUrd), and the temporal bone harvested. Following decalcification, infiltration by BrdUrd- and IgG-positive cells in the ES was studied on frozen sections with H & E and immunohistochemical staining. Results During the secondary immune response in the inner ear against T-dependent antigens, there is increased cellular proliferation in the ES. The proliferated cells may differentiate into immunocompetent cells at the same location. Conclusions These findings indicate that the ES plays an important role in immune response of inner ear.

关键词： Endolymphatic sac Cell proliferation Immune response