Radioimmunoassay of Antiarrhythmic Peptide and Its application

作     者：吴志坚 张永学 WU Zhijian , ZHANG Yongxue Department of Nuclear Medicine, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022

作者机构：DepartmentofNuclearMedicineXieheHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2003年第23卷第1期

页      面：44-47页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 100207[医学-影像医学与核医学] 1006[医学-中西医结合] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 1010[医学-医学技术（可授医学、理学学位）] 100106[医学-放射医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：antiarrhythmic peptide radioimmunoassay blood pressure spontaneous hypertension rats 

摘      要：To establish a specific and sensitive radioimmunoassay (RIA) for antiarrhythmic peptide (AAP) and study serum AAP levels in normal Sprague-Dawley rats (NR) and spontaneous hypertension rats (SHR), AAP-bovine serum albumin (BSA) conjugate was prepared by ammonium bicarbonate method. The New Zealand rabbits were immunized by administering intradermally the conjugate. Then the rabbit anti-AAP serum was produced and iodinated AAP was made by Bolton-Hunter method. The RIA for serum AAP was set up and serum AAP levels in NR and SHR were determined. The minimal detectable range of the AAP RIA was (0.45±0.06) μg/L. The affinity constant for antiserum was 1.05×10 9 L/mol, and the rate of cross-reactivity with atrial peptide (AP) and human growth hormone (hGH) were 0.12 % and 0.20 %, respectively. The mean recovery rate of high, medium and low doses was 97.6 %, and the mean coefficients of variation for intra- and interbath-assay were (6.43±0.85) % and (9.62±1.04) %, respectively. The mean levels of AAP in NR with different age (3 months, 8-10 months and 18-20 months) were (1.75±0.13) μg/L, (1.74±0.11) μg/L and (1.79±0.15) μg/L, respectively, while those in SHR with different age (3 months, 8-10 months and 18-20 months) were (2.38±0.35) μg/L, (2.54±0.25) μg/L and (2.83±0.21) μg/L, respectively. The levels of serum AAP showed a positive correlation with blood pressure (r=0.8667, P0.05). It was indicated that this AAP RIA had high specificity, high accuracy and good reproducibility. The levels of serum AAP had a close relation with blood pressure.