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Isolation and Partial Characterization of a Novel Pollen-specific cDNA with Multiple Polyadenylation Sites from Wheat

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 467-476页

作者： Yong-Feng JIN* and Teng-Fei BIANInstitute of Biochemistry, Zhejiang University, Hangzhou 310029, China Institute of Biochemistry Zhejiang UniversityHangzhou 310029 China

A novel pollen-specific full-length cDNA clone PSG076 was isolated using suppression subtractive hybridization and 5’/3’ RACE techniques. PSG076 was shown to exhibit multi-site polyadenylation by sequencing the 3’ ends of the cDNAs. At least six transcripts with different length were produced from the single gene based on different poly(A) tail attachment sites. However, polyadenylation consensus sequence AAUAAA was not seen at the 3’-untranslated sequence. PSG076 contained a 299 bp 5’ untranslated region and an open reading frame of 663 bp encoding a 221 amino acid peptide with pI of 4.31. A blast search revealed that this sequence did not show a significant similarity to any genes deposited in the public database. Southern blot indicated that PSG076 was a single copy gene. Northern blot and RT-PCR analysis indicated that PSG076 transcripts showed specific expression in mature pollen, and weak or undetectable signals in uninucleate microspore, immature seed, stem, young leave, root and ovary. Further analysis of the expression pattern in gametophyte showed that PSG076 transcripts were undetectable in uninucleate, binucleate microspore and pollen at early stage, and were first detectable and increased rapidly at middle and late stages of pollen development with the maximum level in mature pollen and also expressed in germinating pollen in vivo, suggesting that PSG076might play a role in pollen germination and pollen tube growthin addition to its function in maturation. The evidences gathered in this work indicated that the six different transcripts from the single gene were differentially expressed during pollen development.

关键词： wheat (Triticum aestivum L.) pollen-specific cDNA suppression subtractive hybridization multiple-site polyadenylation differential expression

Identification of a bi-directional promoter from Tomato yellow leaf curl China virus

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Progress in Natural Science:Materials International 2006年第3期16卷 281-286页

作者： GUAN Cuiping and ZHOU Xueping (Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China) Institute of Biotechnology Zhejiang University Hangzhou 310029 China

A bi-directional promoter of Tomato yellow leaf curl China virus (TYLCCNV) was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacterium-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion-sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi-directional promoter revealed that the βC1 protein has no impact on expression of either the virion- or the complementary-sense promoter.

关键词： Tomato yellow leaf curl China virus bi-directional promoter transient expression geminivirus.

Interaction between a Nanovirus-like Component and the Tobacco Curly Shoot Virus/Satellite Complex

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Acta Biochimica et Biophysica Sinica 2005年第1期37卷 25-31页

作者： Pei-Jun WU Xue-Ping ZHOU~* Institute of Biotechnology Zhejiang University,Hangzhou 310029,China Institute of Biotechnology Zhejiang UniversityHangzhou 310029 China

The biological role of DNA1,a nanovirus-like component shown to be associated with thebegomovirus/satellite complex,has not yet been ***,we demonstrated that DNAl of Tobaccocurly shoot virus isolate Y35(TbCSV-Y35)attenuated leaf-curling symptoms induced by TbCSV-Y35 orTbCSV-Y35 plus Y35 DNAβ in the early stage of symptom development and induced leaf cluster at a laterstage of symptom development in Nicotiana benthamiana *** leaf disc assay demonstrated thatTbCSV-Y35 DNA 1 replicated *** blot analysis revealed that TbCSV-Y35 DNA 1 re-duced viral DNA *** DNA accumulation was not reduced when plants were co-inoculatedwith TbCSV-Y35 DNAβ,but the TbCSV-Y35 DNAβ level was dramatically reduced in the presence ofTbCSV-Y35 DNA *** determine whether the interaction between TbCSV/satellite complex and DNA 1 hadisolate specificity,DNAI of TbCSV isolate Y132 was cloned and *** was found to have 75%nucleotide sequence identity with TbCSV-Y35 DNA *** tests showed that TbCSV-Y 132 DNA 1 hadno effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAβ in ***,although Y 132 DNA1 could replicate in these plants.

关键词： Tobacco curly shoot virus DNA1 DNAβ symptom replication interaction

Plant MITEs: Useful Tools for Plant Genetics and Genomics

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Genomics, Proteomics & Bioinformatics 2003年第2期1卷 90-100页

作者： Ying FengAgriculture and Biotechnology College, Zhejiang University, Hangzhou 310029, China Agriculture and Biotechnology College Zhejiang University Hangzhou 310029 China. qzhxue@***

MTTEs (Miniature inverted-repeat transposabie elements) are reminiscence ot non-autonomous DNA (class Ⅱ) elements, which are distinguished from other transposable elements by their small size, short terminal inverted repeats (TIRs), high copy numbers, genie preference, and DNA sequence identity among family members. Although MITEs were first discovered in plants and still actively reshaping genomes, they have been isolated from a wide range of eukaryotic organisms. MITEs can be divided into Tourist-like, Stowaway-like, and pogo-like groups, according to similarities of their TIRs and TSDs (target site duplications). In despite of several models to explain the origin and amplification of MITEs, their mechanisms of transposition and accumulation in eukaryotic genomes remain poorly understood owing to insufficient experimental data. The unique properties of MITEs have been exploited as useful genetic tools for plant genome analysis. Utilization of MITEs as effective and informative genomic markers and potential application of MITEs in plants systematic, phylogenetic, and genetic studies are discussed.

关键词： miniature inverted-repeat transposable element MITE transposable element retrotransposon evolution plant

Mechanisms of granular activated carbon anaerobic fluidized-bed process for treating phenols wastewater

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Journal of Environmental Sciences 2002年第1期14卷 132-135页

作者： LAO Shan gen (Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China) Department of Environmental Engineering Zhejiang University Hangzhou 310029

Granular activated carbon (GAC) anaerobic fluidized bed reactor was applied to treating phenols wastewater. When influent phenol concentration was 1000 mg/L, volume loadings of phenol and COD Cr were 0 39 kg/(m 3·d) and 0 98 kg/(m 3·d), their removal rates were 99 9% and 96 4% respectively. From analyzing above results, the main mechanisms of the process are that through fluidizing GAC, its adsorption is combined with biodegradation, both activities are brought into full play, and phenol in wastewater is effectively decomposed. Meanwhile problems concerning gas liquid separation and medium plugging are well solved.

关键词： granular activated carbon (GAC) anaerobic fluidized bed phenol removal rate

MIXED MODEL APPROACHES FOR ESTIMATING GENETIC VARIANCES AND COVARIANCES

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生物数学学报 1992年第1期 1-11页

作者： Zhu Jun(Zhejiang Agricultural University, Hangzhou 310029)

The limitations of methods for analysis of variance(ANOVA)in estimating genetic variances are discussed. Among the three methods(maximum likelihood ML, restricted maximum likelihood REML, and minimum norm quadratic unbiased estimation MINQUE)for mixed linear models, MINQUE method is presented with formulae for estimating variance components and covariances components and for predicting genetic effects. Several genetic models, which cannot be appropriately analyzed by ANOVA methods, are introduced in forms of mixed linear models. Genetic models with independent random effects can be analyzed by MINQUE(1)method whieh is a MINQUE method with all prior values setting 1. MINQUE(1)method can give unbiased estimation for variance components and covariance components, and linear unbiased prediction (LUP) for genetic effects. There are more complicate genetic models for plant seeds which involve correlated random effects. MINQUE(0/1)method, which is a MINQUE method with all prior covariances setting 0 and all prior variances setting 1, is suitable for estimating variance and covariance components in these models. Mixed model approaches have advantage over ANOVA methods for the capacity of analyzing unbalanced data and complicated models. Some problems about estimation and hypothesis test by MINQUE method are discussed.

关键词： Mixed linear models MINQUE method yariance and covariance estimation random effect prediction.

Effects　of　alkaline　ameliorator　additions　on　the　acidic　buffering　abilities　of　aluminum－rich　alfisols

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Journal of Environmental Sciences 1996年第2期8卷 235-241页

作者： Wu Jiemin Shao Feng(Department of Environmental Science, Zhejiang Agricultural University, Hangzhou 310029 , China) Department of Environmental Science Zhejiang Agricultural University Hangzhou 310029 China

Lime and coal flying ash applications could mitigate pollution from acid deposition and improve the buffering ability of two acidic soils sampled from Zhejiang Province. The results showed that soil alkalinity and acidified buffering capacities (QpHs ) were significantly increased, especially for liming treatment; whereas, the amounts of SO24- adsorbed by treated soils were decreased to some degrees because net negative charges of soil oxide colloidal surfaces were increased with the decrease in soil acidity, although the soil fixation abilities for adsorbed SO24- were not reduced. These facts indicated that after the alkaline ameliorators were applied, the positive and adverse effects resulted from sulfate-type acid deposition were simultaneously existed in these soil treatments.

关键词： acidic alfisol alkaline ameliorator acidified buffering capacity.

Start-up strategies of UASB reactor for treatment of pharmaceutical wastewater

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Journal of Environmental Sciences 2002年第2期14卷 250-254页

作者： ZHENG Ping, HU Bao lan (Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China. Department of Environmental Engineering Zhejiang University Hangzhou 310029

Two start up strategies of upflow anaerobic sludge blanket(UASB) reactor for treatment of pharmaceutical wastewater were investigated. The results showed that both of them were workable. Compared with the strategy that started up the reactor directly using chloromycetin wastewater, the strategy that started up the reactor first using mixed wastewater and then using chloromycetin wastewater could save time by 23%. When the latter strategy was adopted the development of sludge activity fluctuated more largely and its final activity was lower, but the sludge grew faster in the course of start up.

关键词： start up toxic wastewater UASB

Knowledge-Based Classification in Automated Soil Mapping

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Pedosphere 2003年第3期13卷 209-218页

作者： ZHOU BIN and WANG RENCHAOInstitute of Agricultural Remote Sensing and Information Technology Application, Zhejiang University, Hangzhou 310029 (China) Institute of Agricultural Remote Sensing and Information Technology Application Zhejiang University Hangzhou 310029

A machine-learning approach was developed for automated building of knowledgebases for soil resources mapping by using a classification tree to generate knowledge from trainingdata. With this method, building a knowledge base for automated soil mapping was easier than usingthe conventional knowledge acquisition approach. The knowledge base built by classification tree wasused by the knowledge classifier to perform the soil type classification of Longyou County,Zhejiang Province, China using Landsat TM bi-temporal images and CIS data. To evaluate theperformance of the resultant knowledge bases, the classification results were compared to existingsoil map based on a field survey. The accuracy assessment and analysis of the resultant soil mapssuggested that the knowledge bases built by the machine-learning method was of good quality formapping distribution model of soil classes over the study area.

关键词： classification classification tree knowledge-based rule extracting soilmapping