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Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites

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Acta Biochimica et Biophysica Sinica 2005年第12期37卷 819-825页

作者： Ting-Qing GUO~(1,2) Jian-Yang WANG~(1,2) Sheng-Peng WANG~(1,2.3) Xiu-Yang GUO~(1,2) Ke-Wei HUANG3~3 Jun-Ting HUANG~3 Chang-De LU~11 institute of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences.chinese academy of sciences.Shanghai 200031.china 2 Graduate School of the chinese academy of sciences.Shanghai 200031.china 3.serictdtu research institute.chinese academy of agricultural sciences,zhenjiang 212018.china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Graduate School of the chinese academy of sciencesShanghai 200031 China Sericultural research institute Chinese Academy of Agricultural SciencesZhenjiang 212018 China

The gene encoding fibroin light chain protein (FibL) is specifically expressed in the posteriorsilk gland of silkworm and repressed in other *** binding sites of several transcription factorsrevolved in the silk gland transcription specificity of fibl promoter have been recognized,including SGFB,PSGF and *** we report the leak expression of the enhanced green fluorescent protein (EGFP)reporter gene in tissues other than the posterior silk gland in vivo when under the control of a shortened fiblpromoter with deletion of the 5′terminal 41 bp sequence,which is located at -650 nt to -610 nt upstream *** transcription starting *** of silk gland specificity of the promoters was performed by observationof green fluorescence in tissues of silkworm larvae following inter-haemocoelic injection of recombinantAutographa californica multiple nuclear polyhedrosis virus carrying the EGFP reporter gene controlled bydifferent lengths of fibl *** results indicated that availability of the binding sites of several knownfactors,including SGFB,PSGF and BMFA,is not sufficient for intact silk gland transcription specificity offibl promoter,and there are possible inhibitor binding sites in the 41 bp sequence (-650 nt to -610 nt)upstream of the transcription starting site which may be required to repress the activity of fibl promoter inother tissues.

关键词： promoter specificity fibroin light chain silkworm Bombyx mori recombinant AcMNPV

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Structural Analysis of Fibroin Heavy Chain Signal Peptide of Silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 507-513页

作者： Sheng-Peng WANG~(1,2)Ting-Qing GUO~1 Xiu-Yang GUO~1 Jun-Ting HUANG~2 Chang-De LU~(1*) 1 institute of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences,chinese academy of sciences,Shanghai 200031,china 2 Sericultural research institute.chinese academy of agricultural sciences,zhenjiang 212018.china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Sericultural research institute Chinese Academy of Agricultural SciencesZhenjiang 212018 China

To study the minimal length required for the secretion of recombinant proteins and silkproteins in posterior silk gland,the signal peptide(SP)of the fibroin heavy chain(FibH)of silkwormBombyx mori was systematically shortened from the *** effect on the secretion of protein wasobserved using enhanced green fluorescent protein(EGFP)as a *** of EGFP fusion proteinswas examined under fluorescence *** SPs with lengths of 20,18,16 and 12 *** directthe secretion of the reporter,yet those with lengths of 11, 10, 9, 8 and 1 a.a. can not. When the FibH SP wasshortened to 12a.a., the secretion efficiency was decreased slightly and cleavage occurred within *** *** the FibH SP were used,the secretion of fusion protein was normal and the cleavage sitewas between the Gly-Ser linker and Met,the starting amino acid of EGFP. These findings are applicable forthe expression of foreign proteins in silkworm silk *** cleavage site of the SP is discussed andcompared with the predictive results of the SignalP 3.0 online prediction program.

关键词： signal peptide fibroin heavy chain silkworm Bombyx mori rAcMNPV

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Refinement modeling and verification of secure operating systems for communication in digital twins

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Digital Communications and Networks 2024年第2期10卷 304-314页

作者： zhenjiang Qian Gaofei Sun Xiaoshuang Xing Gaurav Dhiman School of Computer Science and Engineering Changshu Institute of TechnologySuzhou215500China University Centre for research and Development Department of Computer Science and EngineeringChandigarh UniversityMohali140413India Department of Computer Science and Engineering Graphic Era Deemed to be UniversityDehradun248002India

In traditional digital twin communication system testing,we can apply test cases as completely as possible in order to ensure the correctness of the system implementation,and even then,there is no guarantee that the digital twin communication system implementation is completely *** verification is currently recognized as a method to ensure the correctness of software system for communication in digital twins because it uses rigorous mathematical methods to verify the correctness of systems for communication in digital twins and can effectively help system designers determine whether the system is designed and implemented *** this paper,we use the interactive theorem proving tool Isabelle/HOL to construct the formal model of the X86 architecture,and to model the related assembly *** verification result shows that the system states obtained after the operations of relevant assembly instructions is consistent with the expected states,indicating that the system meets the design expectations.

关键词： Theorem proving Isabelle/HOL Formal verification System modeling Correctness verification

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Potential land for plantation of Jatropha curcas as feedstocks for biodiesel in china

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Science china Earth sciences 2010年第1期53卷 120-127页

作者： WU WeiGuang 1,2,3,HUANG JiKun 1,3 & DENG XiangZheng 1,3 1 institute of Geographic sciences and Natural Resources research,chinese academy of sciences,Beijing 100101,china 2 Department of Economics and Management,Zhejiang Forestry University,Linan 311300,china 3 Center for chinese agricultural Policy,chinese academy of sciences,Beijing 100101,china institute of Geographic sciences and Natural Resources research Chinese Academy of Sciences Beijing China Department of Economics and Management Zhejiang Forestry University Linan China Center for chinese agricultural Policy Chinese Academy of Sciences Beijing China

As a renewable energy,biofuel has attracted great attention in china and the rest of *** with the national food security,china recently has shifted its biofuel development priority from grain-based to non-grain-based biofuels,including forest-based biodiesel,since *** curcas is one of major biodiesel ***,there is rising debate on availability of land for expanding Jatropha curcas *** overall goal of this paper is to evaluate potential land for Jatropha curcas used as feedstock for biodiesel in *** on remote sensing data on land use,data on meteorological,soil and land slope,and suitable environment for Jatropha curcas plantation,this study uses Agro Ecological Zone method and considers social-economic constraints to evaluate potential suitable land for Jatropha curcas plantation in china's major Jatropha curcas production region,Southwest *** results show that while there are some potential lands to expand Jatropha curcas areas,amount of these lands will hardly meet the government's target for Jatropha curcas-based biodiesels development in the *** may need to reconsider its long-term targets on the development of Jatropha curcas-based biodiesels.

关键词： biodiesel Jatropha curcas potential land suitability evaluation Southwest china

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Suppressed Internal Intrinsic Stress Engineering in High-Performance Ni-Rich Cathode Via Multi layered In Situ Coating Structure

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Energy & Environmental Materials 2024年第2期7卷 58-66页

作者： Jiachao Yang Yunjiao Li Xiaoming Xi Junchao Zheng Jian Yu zhenjiang He School of Metallurgy and Environment Central South UniversityChangsha 410083China Engineering research Center of the Ministry of Education for Advanced Battery Materials Central South UniversityChangsha 410083China Changsha research institute of Mining and Metallurgy Changsha 410083China Ningbo Ronbay New Energy Technol Co Ltd Tanjialing East Rd 39Ningbo 315400China

LiNi_(x)Co_(y)Al_(z)O_(2)(NCA)cathode materials are drawing widespread attention,but the huge gap between the ideal and present cyclic stability still hinders their further commercial application,especially for the Ni-rich LiNi_(x)Co_(y)Al_(z)O_(2)(x>0.8,x+y+z=1)cathode material,which is owing to the structural degradation and particles'intrinsic *** tackle the problems,Li_(0.5)La_(2)Al_(0.5)O_(4)in situ coated and Mn compensating doped multilayer LiNi_(0.82)Co_(0.14)Al_(0.04)O_(2)was *** refinement indicates that La-Mn co-modifying could realize appropriate Li/Ni disorder *** results and in situ XRD patterns reveal that the LLAO coating layer could effectively restrain crack in secondary particles benefited from the suppressed internal *** further improves as NCA-LM2 has superior mechanical *** SEM,TEM,XPS tests indicate that the cycled cathode with LLAO-Mn modification displays a more complete morphology and less side reaction with *** was used to further investigate cathode-electrolyte interface which was reflected by gas ***-LM2 releases less CO_(2)than NCA-P indexing on a more stable *** modified material presents outstanding capacity retention of 96.2%after 100 cycles in the voltage range of 3.0-4.4 V at 1C,13%higher than that of the pristine and 80.8%at 1 C after 300 *** excellent electrochemical performance could be attributed to the fact that the high chemically stable coating layer of Li_(0.5)La_(2)Al_(0.5)O_(4)(LLAO)could enhance the interface and the Mn doping layer could suppress the influence of the lattice mismatch and *** believe that it can be a useful strategy for the modification of Ni-rich cathode material and other advanced functional material.

关键词： compensating doped in situ coating multilayer material Ni-rich cathode materials suppressed internal strain

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Effect of 5'-deletion of Arabi-dopsis profilin2 promoter on its vascular specific expression

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chinese Science Bulletin 2001年第19期46卷 1627-1630页

作者： LIU Yuhui, WANG Zhixing & JIA ShirongBiotechnology research institute, chinese academy of agricultural sciences, Beijing 100081, china Biotechnology research institute Chinese Academy of Agricultural Sciences Beijing China

Based on the published sequence of profilin2 promoter of Arabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5’ -end deletion fragments with length of 1380, 1153, 969 and 597 bp were then fused with gus (uid\) gene respectively. Constructed plant expression vectors were individually transferred into Kalan-choe laciniata and transgenic plants regenerated. GUS his-tochemical assay confirmed that the full-length promoter Pfnl.7 was vascular-specific. Deletion assays showed that profilin2 promoter could be divided into three parts. Deletion of fragment 1 ( -1667 -1380 bp) resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in this region. Fragment 2 located at -1153 - -597 bp strongly inhibited gus gene expression. Fragment 3 ( -597 - -1 bp) is considered as a basic domain of profilin2.

关键词： profilin2 promoter Kalanchoe constitutive expression vascular-specific expression.

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LEAFY HEAD2,which encodes a putative RNA-binding protein,regulates shoot development of rice

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Cell research 2006年第3期16卷 267-276页

作者： Guo Sheng Xiong~(1,3) Xing Ming Hu~2 Yong Qing Jiao~1 Yan Chun Yu~1 Cheng Cai Chu~1 Jia Yang Li~1 Qian Qian~2 Yong Hong Wang~11 State Key Laboratory of Plant Genomics and National Center for Plant Gene research,institute of Genetics and Developmental Biology,chinese academy of sciences,Beijing 100101,china 2 State Key Laboratory of Rice Biology,china National Rice research institute.chinese academy of agricultural sciences,Hangzhou 310006,china 3 Graduate University of chinese academy of sciences,Beijing 100049,china State Key Laboratory of Plant Genomics and National Center for Plant Gene research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China Graduate University of chinese academy of sciences Beijing China State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou China

During vegetative development,higher plants continuously form new leaves in regular spatial and temporal *** with abnormal leaf developmental patterns not only provide a great insight into understanding the regulatorymechanism of plant architecture,but also enrich the ways to its modification by which crop yield could be ***,we reported the characterization of the rice leafy-head2 (lhd2) mutant that exhibits shortened plastochron,dwarfism,reduced tiller number,and failure of phase transition from vegetative to reproductive *** and histologicalstudy revealed that the rapid emergence of leaves in lhd2 was resulted from the rapid initiation ofleafprimordia whereasthe reduced tiller number was a consequence of the suppression of the tiller bud *** molecular and geneticanalysis showed that LHD2 encodes a putative RNA binding protein with 67% similarity to maize *** ofgenome-scale expression profiles between wild-type and lhd2 plants suggested that LHD2 may regulate rice shoot devel-opment through KNOXand hormone-related *** similar phenotypes caused by LHD2 mutation and the conservedexpression pattern of LHD2 indicated a conserved mechanism in controlling the temporal leaf initiation in grass.

关键词： phyllotaxy plastochron LHD2 RNA-binding protein stem elongation plant architecture Oryza sativa L

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Regional differences and determinants of built-up area expansion in china

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Science china Earth sciences 2007年第12期50卷 1835-1843页

作者： HUANG JiKun1,2,ZHU LiFen3,1 & DENG XiangZheng1,2 1 Center for chinese agricultural Policy,chinese academy of sciences,Beijing 100101,china 2 institute of Geographic sciences and Natural Resources research,chinese academy of sciences,Beijing 100101,china 3 research Center of the Economy of the Upper Reaches of the Yangtze River,Chongqing Technology and Business University,Chongqing 400067,china Center for chinese agricultural Policy Chinese Academy of Sciences Beijing China institute of Geographic sciences and Natural Resources research Chinese Academy of Sciences Beijing China research Center of the Economy of the Upper Reaches of the Yangtze River Chongqing Technology and Business University Chongqing China

Based on remote sensing data on land use provided by the chinese academy of sciences and socio-economic data collected by the authors,this paper analyzes the trends and regional differences in built-up area(BUA) expansion in china from the late 1980s to 2000,and empirically estimates the major determinants of BUA expansion in different regions in 1996―*** 1989―2000,although china's overall BUA expansion accelerated,the trends differed significantly among *** expansion in the central and western regions accelerated significantly,but it slowed down considerably in the east-ern *** estimation results from our econometric analysis reveal that BUA expansion in the eastern region reached a period when economic growth had no further significant impact on per capita BUA,the land utilization in this region has become more intensive with further expansion of the *** the central and western regions,the BUA has expanded remarkably due to the relatively more flexible land development policies and the relatively cheap land ***,as the econ-omy continues to grow rapidly,policies relating to BUA expansion and cultivated land reductions may face more serious challenges in the central and western regions.

关键词： built-up area, land use, regional difference, determinants, china

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Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 589-596页

作者： Jian-Zhong YI1, Ming-Qiu LIU1, Cai-Zhu ZHU2, Qiang ZHANG1, Zu-Tian SHENG3, Qing-Yun DU3, Wei-Yao YAN1, and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai 200433, china 1The Veterinary institute of chinese academy of agricultural sciences, Lanzhou 730046, china 2The Virus research institute of Zhejiang academy of agricultural sciences, Hangzhou 310021, china The Veterinary institute of chinese academy of agricultural sciencesLanzhou 730046 China The Virus research institute of Zhejiang academy of agricultural sciencesHangzhou 310021 China State Key Laboratory of Genetics Engineering Life Science School Fudan UniversityShanghai 200433 China

In this study, two DNA fragments encoding amino acid (141–160)-(21–40)-(141–160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138–160)-(21–40)-(138–160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine- IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 μg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.

关键词： foot-and-mouth disease FMDV serotype O FMDV serotype A VP1 protein bivalent vaccine guinea pig

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Analysis of Developmental Proteome at Egg Stage of Drone Honeybees (A. m. ligustica)

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agricultural sciences in china 2010年第3期9卷 392-400页

作者： FANG Yu and LI Jian-ke institute of Apicultural research, chinese academy of agricultural sciences/Key Laboratory of Pollinating Insect Biology, Beijing 100093, P.R.china institute of Apicultural research Chinese Academy of Agricultural Sciences/Key Laboratory of Pollinating Insect Biology Beijing 100093 P.R.China

An investigation on the proteome of drone egg development of native Italian bee （Apis mellifera ligustica Spinola,1806） was carried out in order to prove up the characteristics in protein expression and regulation at egg stage and open out the molecular mechanism of the development. The experiment was carried out by two-dimensional gel electrophoresis. The results showed that there were 200, 242 and 233 proteins in a wide rang of molecular weight （12.42-169.60 kDa） and in a relatively narrow scope of pI （4.50-9.00） detected on day 1, day 2 and day 3, respectively, during the developmental process of the drone egg. Meanwhile, 164 protein spots were resolved at all the images （i.e., the protein was consistently expressed） along with the egg development, among which 7 were significantly up-expressed （P 〈 0.05） and 4 were significantly down-expressed （P 〈 0.05） while 79 had no significant differences （P 〉 0.05）. In addition, the specific proteins expressing proteins on day 1, day 2 and day 3 were 11, 18 and 18, respectively. Besides, 17 proteins expressed both on day 1 and day 2 but silenced on day 3, and 43 proteins expressed both on day 2 and day 3 but silenced on day 1, while only 8 proteins expressed both on day 1 and day 3 but silenced on day 2. The results indicate that 2-d-old eggs are at the most active expressional stage in the development of drone egg. The protein expressing at all images suggests that it should be indispensable for drone egg development, but their expression pattern is different. The proteins expressing at a specific age of egg suggest that specific proteins are needed in different developmental stages to regulate. And there are more house-keeping proteins in the developmental process of the drone egg than that of worker egg, and it will provide more targets for gene improvement.

关键词： honeybees drone egg two-dimensional gel electrophoresis proteome

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